Background: Pseudocyst formations around the pancreatic stump may occur at late-onset after distal pancreatectomy with staple closure. Although this finding may be associated with pancreatic fistula, its clinical significance is not clear. Object: Between January 2006 and June 2017, distal pancreatectomy was performed on 154 patients. Among them, 82 patients who underwent pancreatectomy with staple closure were retrospectively studied. Result: Eighty patients (97.6%) were followed with CT and MRI postoperatively and subjected to be analyzed. 20 cases (25%) showed pseudocyst formation at the pancreatic stump. On the other hand, 7 (9.7%) of 72 cases in which the pancreas was closed with hand sewing during the same period showed pseudocyst formation. There was a significantly higher rate of pseudocyst formation in the group with staple closure (P=0.013). The postoperative drain amylase values (POD 1, 3, 4) were significantly higher (8154 vs 2714, 1380 vs 542, 848 vs 358, respectively) in the pseudocyst formation group than the non-formation group. Univariate analysis for the prediction of pseudocyst formation revealed that there were no significant differences in pancreatic thickness or hardness, the diameter of the main pancreatic duct, ISGPF grade, or the number of days for drain removal. Conclusion: Distal pancreatectomy with staple closure may induce the formation of pseudocysts at the pancreatic stump. Although there was no significant association with pancreatic fistula, the value of amylase in drains was associated with pseudocyst formation.