Standardized Ileal Digestibility Of Amino Acids Research Articles

Standardized ileal digestibility of amino acids differs among sources of bakery meal when fed to growing pigs.

A multistate experiment involving universities in IL, IN, KY, and MN was conducted as a part of the research efforts by the North-Central Coordinating Committee-42 on swine nutrition. The null hypothesis that there are no differences in the standardized ileal digestibility (SID) of amino acids (AA) among different sources of bakery meal was tested. Eleven sources of bakery meal were procured from swine-producing states in the United States and each source was included in one diet as the sole source of AA. A N-free diet was prepared as well. Diets were prepared in one batch and divided into four sub-batches that were subsequently distributed to the four participating universities. At each university, diets were fed to 12 pigs that had a T- cannula installed in the distal ileum. Pigs were allotted to incomplete Latin square designs with 12 pigs and 4, 5, or 6 periods for a total of 21 replicate pigs per diet. Each period lasted 7 d with ileal digesta being collected from the cannulas on days 6 and 7. Samples were analyzed for AA and the SID of each AA was calculated. Results indicated that there were differences (P < 0.001) in the SID of all AA except Pro among the 11 sources of bakery meal. The differences in SID of AA observed in this experiment were greater than what is usually observed among sources of the same ingredient, indicating that there is more variability among sources of bakery meal than among different sources of other ingredients. This is likely a consequence of different raw materials being used in the production of different sources of bakery meal. Regardless of source of bakery meal, the AA with the least SID was Lys indicating that some of the raw materials in the product streams used to generate the bakery meals may have been overheated. Additionally, the Lys:crude protein ratio in each source of bakery meal was not a good predictor of the SID of Lys, which likely reflects the different raw materials being included in the different meals. In conclusion, the SID of AA varies among different sources of bakery meal and the SID of Lys is less than the SID of all other indispensable AA.

Comparison of standardized ileal digestibility of amino acids in faba beans and field peas in broiler chickens and pigs.

An experiment, using the same set of five diets was conducted to determine and compare the standardized ileal digestibility (SID) of amino acids (AA) in faba beans and three cultivars of field peas in broiler chickens and pigs. Four test diets were prepared to contain faba beans, DS-Admiral field peas, Hampton field peas, or 4010 field peas as the sole source of nitrogen. A nitrogen-free diet (NFD), as the fifth diet, was prepared to estimate the basal endogenous losses of AA to determine the SID of AA in the test ingredients. A total of 416 male broiler chickens with an initial body weight (BW) of 951 ± 111 g were allotted to five diets in a randomized complete block design with BW as a blocking factor on day 21 posthatching. There were 8 replicate cages with 10 birds per cage for diets containing test ingredients and 12 birds per cage for NFD. All birds were allowed ad-libitum access to feed for 5 days. On day 26 posthatching, all birds were euthanized by CO2 asphyxiation and digesta contents were collected from the distal two-thirds of the ileum. Twenty barrows with an initial BW of 30.2 ± 1.58 kg, surgically fitted with T-cannulas in the distal ileum, were divided into four blocks according to BW and each block was assigned to a 5 × 2 incomplete Latin Square design that consisted of five dietary treatments and two experimental periods. For each experimental period, there was a 5-day adaptation period followed by a 2-day collection of ileal digesta samples. The data were analyzed as a 2 × 4 factorial treatment arrangement with the effect of species (i.e., broiler chickens and pigs) and test diets (i.e., four test ingredients). For broiler chickens, the SID of Lys in faba beans, DS-Admiral field peas, and Hampton field peas were above 90% but in 4010 field peas, it was 85.1%. The SID of Lys in faba beans, DS-Admiral field peas, and Hampton field peas for pigs was above 80% but was 78.9% in 4010 field peas. The respective SID of Met in faba beans, DS-Admiral field peas, Hampton field peas, and 4010 field peas were 84.1%, 87.3%, 89.8%, and 72.1% for broiler chickens, and 71.5%, 80.4%, 81.8%, and 68.1% for pigs. The SID of AA in 4010 field peas was the least (P < 0.05) for chickens, but in pigs, it was comparable with faba beans. In conclusion, the SID of AA in faba beans and field peas was greater in broiler chickens compared with pigs and there was a cultivar effect.

Nutritional value of high protein ingredients fed to growing pigs in comparison to commonly used protein sources in swine diets.

Two experiments were conducted to test the hypothesis that 2 high protein dried distillers with solubles and yeast mass added (GDDY) products fed to growing pigs had comparable amino acid (AA) digestibility and metabolizable energy (ME) to feeds commonly used in swine diets. In Exp. 1, 7 barrows with initial body weight (BW) of 25 ± 0.8kg were fitted with a simple T-cannula at the distal ileum to allow for digesta collection. Experimental diets were N-free diet and 6 cornstarch-based diets containing 6 ingredients as the sole source of AA: spray dried GDDY, ring dried GDDY, corn distillers dried grains with solubles (DDGS), soybean meal (SBM), enzymatically treated soybean meal (ESBM), and Fish meal (FM) provided at 4% of BW. The experiment was conducted as a 7 × 7 Latin square design with 7 collection periods of 7d (5d adaptation and 2d ileal digesta collection). In Exp. 2, a total of 28 barrows (28.8 ± 1.4kg BW) were used in a 2-period switch back design with 7 diets and 4 replicate pigs in each period (n=8 reps/diet). Experimental diets were a corn-based basal diet and 6 corn-based diets containing spray dried GDDY, ring dried GDDY, DDGS, SBM, ESBM and FM. Fecal and urine samples were collected using the marker-to-marker approach for 5d after 7d of adaptation to determine ME concentration. Overall, SID values were within the mean +/- SD of NRC (2012) values for all ingredients evaluated. The SID of AA was greater (P < 0.05) in ESBM than the other protein feedstuffs (90.09% vs. 78.71 - 81.51%). There were not significant differences in SID of AA (P > 0.05) in SBM, FM, spray dried GDDY and ring dried GDDY (81.49, 78.71, 81.52, and 79.20 %). With respect to the most common first limiting AA for swine, the SID of Lys was greater (P < 0.05) in spray dried GDDY than ring dried GDDY and DDGS (83.56 vs. 77.33 and 68.53 %, respectively). There were not significant differences (P > 0.05) for ME in corn (3313 Kcal/kg), ESBM (3323 Kcal/kg), and FM (3454 Kcal/kg) when compared with spray dried GDDY and ring dried GDDY (3995 Kcal/kg and 3442 Kcal/kg respectively). However, spray dried GDDY had greater DE and ME when compared to SBM. Collectively, this study demonstrates that GDDY products have an AA profile and digestibility comparable to SBM; ME in GDDY products is not different than corn. Therefore, GDDY has potential as a feed ingredient for pigs, which could provide an alternative source of protein and energy in swine diets.

Effects of corn hardness and drying temperature on digestibility of energy and nutrients in diets fed to growing pigs.

Two experiments were conducted to test the hypothesis that corn kernel hardness and drying temperature influence the ileal digestibility of starch and amino acids (AA), as well as apparent total tract digestibility (ATTD) of gross energy (GE) and total dietary fiber (TDF) in diets for growing pigs. Two corn varieties with average or hard endosperm were grown and harvested under similar conditions, and after harvest each variety was divided into 2 batches that were dried at 35 oC and 120 oC, respectively. Therefore, four batches of corn were used. In experiment 1, ten pigs (67.00 ± 2.98kg) with a T-cannula installed in the distal ileum were allotted to a replicated 5 × 5 Latin square design with five diets and five periods giving ten replicates per diet. A nitrogen-free diet and four diets containing each source of corn as the only AA source were formulated. Results indicated that neither variety of corn nor drying temperature influenced apparent ileal digestibility of starch in the grain. The standardized ileal digestibility of most AA was less (P < 0.05) in corn dried at 120 oC compared with corn dried at 35 oC resulting in concentrations of most standardized ileal digestible AA being less (P < 0.05) in corn dried at 120 oC than in corn dried at 35 oC. In experiment 2, 40 pigs (20.82 ± 1.74kg) were housed in metabolism crates and allotted to four diets with ten replicate pigs per diet. The four corn-based diets used in experiment 1 were also used in experiment 2. Feces and urine were collected using the marker-to-marker approach with 5-day adaptation and 4-day collection periods. Results indicated that diets containing hard endosperm corn had greater (P < 0.05) ATTD of TDF than diets containing average endosperm corn. The ATTD of GE in hard endosperm corn was also greater (P < 0.05), and concentrations of digestible energy and metabolizable energy in hard endosperm corn were greater (P < 0.01) than in average endosperm corn. Diets containing corn dried at 120 oC had greater (P < 0.05) ATTD of TDF compared with diets containing corn dried at 35 oC; however, drying temperature did not influence the ATTD of GE. In conclusion, endosperm hardness did not influence the digestibility of AA and starch; however, drying corn at 120 oC reduced digestible AA concentrations. Hard endosperm corn had greater ATTD of GE and TDF, but drying temperature did not influence energy digestibility.

Enhancing the nutritive value of canola meal for broiler chickens through enzymatic modifications.

Enzymatic modification of canola meal (CM) is a potential method to enhance its nutritional value as it can depolymerize nonstarch polysaccharides (NSP) and mitigate its potential antinutritive properties. Based on the previous studies, pectinase A (PA), pectinase B (PB), xylanase B (XB), and invertase (Inv) were used for the enzymatic modifications. The highest NSP depolymerization ratio was obtained when 4 g/kg of each PA, PB, and XB, and 0.2 g/kg of Inv were used during 48 h incubation at 40 °C. In the current study, changes in pH, simple sugars, sucrose, oligosaccharides, and NSP contents during the enzymatic modification (CM+E) of CM were measured and compared to Control (CM) without enzymes addition or with the addition of bacteriostat sodium azide (CM+E+NaN3). The results showed that spontaneous fermentation occurred during incubation. After incubation, the pH of the slurry decreased, lactic acid was produced, phytate disappeared, and the concentration of simple sugars decreased substantially. The NSP of the slurry was progressively depolymerized by the enzyme blend. The chemical composition and nutritive value of enzymatically-modified CM (ECM) were evaluated. Ross 308 broilers were randomly assigned to 18 cages of six birds each for the standardized ileal amino acid digestibility (SIAAD) and nitrogen-corrected apparent metabolizable energy (AMEn) assay. A corn/soybean meal-based basal diet formulated to meet Ross 308 breeder recommendations and two test diets contained 70% of the basal diet and 30% of CM or ECM, respectively, were fed to Ross 308 from 13 to 17 d of age. No difference was observed between SIAAD of CM and ECM. The AMEn value of ECM was 2118.0 kcal/kg on a dry matter basis which was 30.9% greater (P < 0.05) than the CM.

Effects of feeding levels on ileal amino acid digestibility of extruded full fat soybeans in nongestating sows.

The objectives of this study were to investigate the effect of feeding levels on amino acid (AA) digestibility of extruded full fat soybeans (EFSB) fed to nongestating sows and to provide a reference for setting feed intake level when evaluating the quality of nutrients in the feed ingested by sows. Twelve nongestating sows (parity 3 to 5) were fitted with a T-cannula at the distal ileum. After recovery, sows were assigned to a replicated 6 × 3 incomplete Latin square design using two diets (nitrogen-free and EFSB) and three levels of feed intake (1.3, 2.0, and 3.4 times the maintenance requirement for metabolizable energy (ME)). The design included six dietary treatments and three periods, and each period contained two replicates for a total of six replicate sows per treatment. All diets contained 0.3% chromic oxide as an indigestible marker. In each period, ileal digesta samples were collected continuously for 12 h on days 6 and 7 after 5 d of acclimation to the experimental diet. Results of the experiment indicated that different feeding levels (1.3, 2.0, and 3.4 times the maintenance requirement for ME) had no effects on apparent ileal digestibility (AID) of AA and standardized ileal digestibility (SID) of AA, but feeding level did affect the endogenous AA loss estimated using the nitrogen-free diet method. Endogenous phenylalanine excretion was greater with 1.3 times than with 3.4 times the maintenance requirement for ME (P = 0.03), and endogenous tyrosine excretion was greater with 1.3 and 2.0 times than with 3.4 times the maintenance requirement for ME (P = 0.01). Increasing feed consumption resulted in greater loss of total endogenous AAs and crude protein (CP). In conclusion, feeding levels of 1.3 to 3.4 times the maintenance requirement for ME did not affect the AID and SID of AA of EFSB fed to nongestating sows.

Nutritional value of a new source of cheese coproduct fed to weanling pigs.

Three experiments were conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) and metabolizable energy (ME) in a the cheese coproduct are greater than in fish meal or enzyme-treated soybean meal (ESBM). The second objective was to test the hypothesis that pigs fed a diet containing cheese coproduct will have growth performance that is not different from that of pigs fed other sources of protein. In experiment 1, eight ileal-cannulated barrows (11.0 ± 0.4 kg) were allotted to a replicated 4 × 4 Latin square design with four diets and four periods and two pigs per diet in each period. The four diets included an N-free diet and three diets that contained ESBM, fish meal, or the cheese coproduct as the source of AA. Results indicated that the cheese coproduct had greater (P < 0.05) SID of most AA compared with ESBM and fish meal. In experiment 2, 32 weanling barrows (14.0 ± 1.1 kg) were housed individually in metabolism crates and randomly allotted to one of four diets. A corn-based diet and three diets that contained corn and ESBM, fish meal, or cheese coproduct were formulated. Feces and urine were collected quantitatively. The ME in cheese coproduct was greater (P < 0.05) than in ESBM and fish meal. In experiment 3, 128 weaned pigs (6.2 ± 0.6 kg) were allotted to a randomized complete block design with four treatments and 8 replicate pens per diet. Phase 1 diets that contained 0%, 6.65%, 7.35%, or 14% cheese coproduct were fed from days 1 to 14 and a common phase 2 diet without cheese coproduct was fed from days 15 to 28. Individual pig weights were recorded at the beginning of the experiment, on days 14 and 28, and daily feed allotments were also recorded. Two blood samples were collected from 1 pig per pen on day 14 to analyze for blood urea N, albumin, total plasma protein, peptide YY, immunoglobulin G, tumor necrosis factor-α, interleukin-6, and interleukin-10. No differences were observed in average daily gain among treatments, but there was a tendency (P < 0.10) for total protein on day 14 to increase as cheese coproduct increased in the diets. In conclusion, the cheese coproduct used in this experiment has a greater SID of AA and greater ME than ESBM and fish meal and the cheese coproduct may be included in prestarter diets for weanling pigs without negatively impacting growth performance or indicators of intestinal health.

Effects of Aspergillus oryzae prebiotic on dietary energy and nutrient digestibility of growing pigs.

The objective of this study was to determine the effects of Aspergillus oryzae prebiotic (AOP) on nutrient digestibility in growing pigs fed high-fiber diets. Eighteen growing barrows (initial body weight = 50.6 ± 4.9 kg) were surgically equipped with a T-cannula at the distal ileum. Corn and soybean meal-based diets were formulated with fiber from cereal grain byproducts corn (distillers dried grains with solubles, DDGS), rice (rice bran, RB), or wheat (wheat middlings, WM) to meet or exceed all nutrient requirements for 50 to 75 kg growing pigs. Three additional diets were formulated to contain 0.05% AOP supplemented at the expense of corn in the DDGS diet (DDGS + AOP), RB diet (RB + AOP), and WM diet (WM + AOP). All diets contained 0.5% of titanium dioxide as an indigestible marker. Pigs were allotted randomly to a triplicated 6 × 2 Youden square design with six diets and two successive periods. Ileal digesta and fecal samples were collected for 2 d after a 21-d adaptation period, and dry matter (DM), gross energy (GE), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), and ash were analyzed to calculate apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD). Standardized ileal digestibility (SID) of amino acids (AA) was calculated by correcting AID with basal endogenous AA losses from the same set of pigs. Pigs fed the DDGS+AOP diet had greater (P < 0.05) AID of EE compared with those fed the DDGS diet. However, supplementation of AOP did not (P > 0.05) affect AID of GE, DM, CP, NDF, ash or SID of AA of any high-fiber diet. Supplementation of 0.05% AOP increased (P < 0.05) ATTD of DM, GE, CP, NDF, and ash in DDGS, RB, and WM diets. Diet digestible energy was 35 kcal/kg greater (P < 0.05) in pigs fed AOP supplemented diets compared with those fed diets without AOP. In conclusion, supplementation of AOP increased ATTD of nutrients and energy value in high-fiber diets containing DDGS, RB, or WM.

Determination and prediction of standardized ileal amino acid digestibility of wheat in broilers

This experiment evaluated the standard ileal digestibility (SID) of amino acids (AA) in 8 different sources of wheat fed to broilers and established prediction equations based on the chemical properties of wheat. A total of five hundred forty 1-day-old broilers were tested in 2 stages (from d 10 to 13 and from d 25 to 28). On d 13, 324 broilers were randomly assigned to 9 diets (6 replicate cages, 6 broilers per cage); on d 28, 216 broilers were randomly assigned to 9 diets (6 replicate cages, 4 broilers per cage). The 9 experimental diets included 8 test diets and 1 nitrogen-free diet. Titanium dioxide was added as an exogenous indicator at 0.5% of the diet. In 8 wheat samples, the mean values of total amino acids (TAA), dispensable amino acids (DAA), and indispensable amino acids (IAA) were 12.16% (CV 13.70%), 7.97% (CV 15.49%), and 4.20% (CV 11.47%). On d 13, the lowest SID of AA was Lys (86.71%), and the highest was Pro (97.98%). On d 28, the lowest SID of AA was His (81.31%), and the highest was Pro (96.83%). There was an effect of wheat source on the SID of AA except for Trp (P < 0.05); the broiler age had an effect on the SID of AA except for Tyr (P < 0.05); the SID of most AA were higher at d 13 compared to d 28. At d 13, the SID of AA was correlated with CP, NDF, and ST (P < 0.05). At d 28, the SID of AA was correlated with EE, Ash, ADF, and NDF (P < 0.05). The R2 value of stepwise regression equations to predict the SID of AA at d 13 was highest for Leu (R2=0.972), lowest for Asp (R2=0.785); at d 28 was highest for Gly (R2=0.995), lowest for His (R2=0.678). In conclusion, this experiment showed that the chemical properties of wheat can be used to establish accurate equations for predicting the SID of AA. This made it more efficient to obtain the SID of AA for wheat.

Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.

An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs. A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA). Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets. Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods. There were three pigs per diet in each period; therefore, there were nine replicate pigs per diet. The initial 5 d of each period was considered an adaptation period to the diet. For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures. Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets. Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7% to 79.8%. Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets. Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as the inclusion level of phytase increased in the diets. Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets. A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets was observed as the concentration of microbial phytase increased in the diets. The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased. In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.

Apparent and standardized ileal amino acid digestibilities of corn, wheat, soybean meal, and corn gluten meal in quail chicks

Two experiments were conducted to measure the apparent and standardized ileal digestibilities (AID and SID) of amino acid (AA) of corn, wheat, soybean meal (SBM), and corn gluten meal (CGM) in growing Japanese quail from 14 to 18 (Exp. 1) and 28 to 32 (Exp. 2) d of age. The basal endogenous losses of amino acids were measured by the use of N-free diet. The birds were fed on standard diet before the use of experimental diets. The experimental diets (four ingredients) and N-free diet were randomly assigned to 5 replicate pens (30 birds per pen) and fed for 5 consecutive days. The ileal digesta were collected on d 18 and 32 for the Exp. 1 and Exp. 2, respectively. AID of lysine (Lys) in corn (P=0.047), SBM (P < 0.001), and CGM (P < 0.001); AID of threonine (Thr) in corn (P < 0.001), SBM (P < 0.001), and CGM (P=0.075); and AID of isoleucine (Ile) in wheat (P < 0.001), SBM (P=0.002), and CGM (P < 0.001) were increased as the birds aged. However, AID of methionine (Met) in corn (P < 0.001) and CGM (P < 0.001), AID of arginine (Arg; P < 0.001) and valine (Val; P < 0.001) in CGM were lower in younger quails. Among indispensable amino acids, the basal endogenous losses of Thr, Val, and Arg decreased by age (P < 0.001). The average of SID of Lys, Ile, Met, Val, Thr, Arg, leucine (Leu), and histidine (His) for corn, wheat, SBM, and CGM were estimated as 83, ND, 89.4, 89.4, 92.1, 90.2, 91.9, and 90.8%; 92.7, ND, 89.1, 93.9, 87.4, 90.2, 89.8, and 88.1%; 90.3, 91.8, 94.3, 90.4, 86.5, 94.0, 84.3, and 95.0%; 82.6, ND, 74.1, 79.6, 84.4, 90.6, 85.2, and 82.4%, respectively. Based on the present study, the AID and SID coefficients of indispensable AA should be adjusted for age classes in Japanese quail during the growing period.

Effects of Fat Pre-Emulsification on the Growth Performance, Serum Biochemical Index, Digestive Enzyme Activities, Nutrient Utilization, and Standardized Ileal Digestibility of Amino Acids in Pekin Ducks Fed Diets with Different Fat Sources.

Simple SummaryEmulsifiers, which can reduce fat chylomicrons and improve fat emulsification, are currently widely used in animal feed to increase the utilization of fats and improve animal growth performance. However, the traditional method of adding emulsifiers directly to feed has shown inconsistent effects on livestock and poultry. Moreover, China is the largest duck-producing country, and it can, therefore, be anticipated that many duck processing enterprises will produce a large amount of duck fat. However, there is no information about the effects of duck fat as a fat source for Pekin duck. Considering the increase in oil prices, this study was conducted to investigate the effects of fat pre-emulsification (preE), which is a new method to improve the utilization of dietary oil, on Pekin ducks fed diets with different fat sources. The obtained results revealed that fat preE contributed to the utilization of dietary nutrients, serum lipid metabolism, intestinal digestive enzyme activities, and liver health, thereby improving the growth performance of ducks; duck fat has higher bioavailability for ducks based on dietary ether extract (EE) utilization.This experiment was conducted to evaluate the effects of fat pre-emulsification on growth performance, the serum biochemical index, intestinal digestive enzyme activities, nutrient utilization, and the standardized ileal digestibility of amino acids (SIDAA) in Pekin ducks fed diets containing different fat sources. Three hundred and twenty healthy ten-day-old Pekin male ducks (409 ± 27 g) were assigned to a 2 × 2 factorial design and given one of two types of poultry fat (duck fat or a mixed type of fat composed of chicken fat and duck fat in a 1:1 ratio) that had been pre-emulsified or not. This resulted in four treatments of eight replicates, with each replicate having ten ducks. The results showed that fat pre-emulsification (preE) significantly increased (p < 0.05) body weight and body weight gain and decreased (p < 0.05) the feed-to-gain ratio, the liver index, the activity of aspartate aminotransferase (AST) and the concentration of total cholesterol (TC) in the serum. Fat preE also tended to decrease the activity of lipase (p = 0.07) and significantly reduced (p < 0.05) the activity of trypsin in the duodenum. The utilization of dietary dry matter, ether extract (EE), energy, and total phosphorus, as well as apparent metabolizable energy (AME) and the SID of serine (p = 0.090), were improved by fat preE. Duck fat markedly increased (p < 0.05) the serum TC concentration and the utilization of dietary EE; however, it also tended to decrease the serum triglyceride (TG) concentration (p = 0.09) and markedly decreased (p < 0.05) the activity of trypsin in the jejunum and duodenum. These results indicated that fat preE contributed to the utilization of dietary nutrients, serum lipid metabolism, intestinal digestive enzyme activities, and liver health, thereby improving the growth performance of ducks. Duck fat has higher bioavailability for ducks based on dietary EE utilization.

Partial Replacement of Soybean Meal with Canola Meal or Corn DDGS in Low-Protein Diets Supplemented with Crystalline Amino Acids-Effect on Growth Performance, Whole-Body Composition, and Litter Characteristics.

Simple SummaryDietary protein reduction with amino acid supplementation is a nutritional approach to reducing environmental pollution. Soybean meal is nutritionally superior to alternative protein feedstuffs, such as corn distillers dried grain with solubles (cDDGS) and canola meal (CM), because of its relatively good balance of amino acids. This study investigated the usage of CM or cDDGS compared to SBM in low-protein broiler diets and their effects on growth performance, carcass yield, whole-body composition, and litter characteristics. The results demonstrated that replacing soybean meal with cDDGS or CM negatively affected the performance of the birds, whereas reducing dietary protein reduced the litter surface ammonia, irrespective of protein feedstuff. Therefore, complete replacement of SBM with cDDGS or CM in low-protein diets is not feasible for optimum performance in broiler chickens.A 42-day study was conducted to explore the application of supplemental amino acids (AA) in low-protein diets with soybean meal (SBM), canola meal (CM) or corn distillers dried grain with solubles (cDDGS) as the main protein feedstuffs. The responses of interest were growth performance, carcass yield, whole-body composition, litter ammonia and litter N. On d 0, a total of 540 Cobb 500 (off-sex) male broilers were allocated to 36 floor pens. All the birds received one starter diet that met nutrient requirements during the first 10d. Thereafter, six experimental diets were provided in grower and finisher phases. The diets included a positive control (PC): a corn–SBM diet with adequate protein. The protein level of the negative control (NC) was decreased by 45 g/kg relative to the PC. The next two diets had the same protein levels as the NC but with cDDGS added at 50 or 125 g/kg. The last two diets had the same CP as the NC but with CM added at 50 or 100 g/kg. All the low-protein diets had the same level of standardized ileal digestible indispensable AA according to Cobb 500 recommended level. Gly and Ser were added as sources of non-specific N. The dietary protein reduction in corn–SBM diets at both phases decreased (p < 0.05) weight gain and increased (p < 0.05) feed conversion ratio (FCR). Increasing levels of cDDGS or CM, at a constant CP level, linearly decreased (p < 0.05) the weight gain and feed intake, whereas increasing CM level linearly increased (p < 0.05) FCR in the grower and finisher phases. The eviscerated and carcass yields decreased, whereas the fat yield increased (p < 0.05) with reduced protein in corn–SBM diet. Increasing levels of cDDGS and CM at a constant CP level quadratically decreased (p < 0.05) the eviscerated weight, whereas the fat weight linearly decreased (p < 0.05) with increasing levels of cDDGS and CM. The birds receiving the PC diet had a lower (p < 0.05) lean muscle (%) and a higher fat (%) compared to birds receiving the NC diet at d 21. However, on d42, birds receiving the PC diet had decreased (p < 0.05) bone mineral density, bone mineral content and lean weight compared to those receiving the NC diet. The litter ammonia increased (p < 0.05) with the increasing levels of protein in the SBM diets. In conclusion, 50 g/kg inclusion levels of CM and cDDGS at the same low-protein levels as SBM produced a similar growth response to the NC, whereas higher levels were detrimental. Hence under the conditions of the current experiment, complete replacement of SBM with DDGS or CM in low-protein diets was not feasible.

PSXVI-13 Standardized Ileal Digestibility of Amino Acids in Fish Meal, Defatted Black Soldier fly Larva Meal and Adult Black Soldier fly fed to Pigs

Abstract The objective was to determine standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in fish meal, defatted black soldier fly (BSF; Hermetia illucens) larva meal, and adult BSF fed to pigs. Eight barrows with an initial body weight of 72.6 kg (SD = 10.4) fitted with T-cannulas in the distal ileum were individually housed in pens that were equipped with a feeder and a nipple drinker. The pigs were allotted to a replicated 4 × 5 incomplete Latin square design with 4 diets and 5 periods. Three experimental diets containing fish meal (68.6% CP, 5.24% Lys, and 7.5% ether extract), defatted BSF larva meal (59.5% CP, 3.82% Lys, and 7.6% ether extract), and adult BSF (59.0% CP, 2.80% Lys, and 26.8% ether extract) as the sole source of AA were prepared. Additionally, a nitrogen-free diet was formulated to determine the basal endogenous losses of CP and AA. All diets contained 0.5% chromium oxide as an indigestible index. Each period consisted of 4 days of adaptation and 2 days of ileal digesta collection. The SID of CP in defatted BSF larva meal was less (73.8% vs. 88.3%; P &amp;lt; 0.05) than that in fish meal but was greater (P &amp;lt; 0.05) than that in adult BSF (56.1%). The SID of all indispensable AA except for Met and Phe in defatted BSF larva meal was less (P &amp;lt; 0.05) than that in fish meal. The SID of all AA in defatted BSF larva meal was greater (P &amp;lt; 0.05) than that in adult BSF. In conclusion, AA in defatted BSF larva meal are less digestible than those in fish meal when fed to pigs but are more digestible than those in in adult BSF.

270 Effects of Different Watering Options on Standardized Ileal Digestibility of Amino Acids and net Energy in Diets fed to Growing Pigs

Abstract Two experiments were conducted to test the hypothesis that different watering options affect the standardized ileal digestibility (SID) of amino acids (AA), apparent total tract digestibility (ATTD) of dry matter (DM) and gross energy (GE), and concentration of net energy (NE) by growing pigs. A corn-soybean meal-based diet was used in both experiments. There were 3 treatments: 1) dry feeder plus a water nipple located outside the feeder, 2) wet-dry feeder with the water nipple inside the feeder, and 3) combined system with a water nipple both inside the feeder and also an outside nipple. In Exp. 1, pigs (n = 6; initial weight = 27.53 kg) that were equipped with a T-cannula in the distal ileum were allotted to the 3 treatments using a replicated 3 × 3 Latin square design with 6 pigs and three 7-d periods. All pigs were fed an N-free diet for an additional period to determine basal endogenous losses of AA. Chromic oxide was added to all diets and ileal digesta were collected according to standard procedures. In Exp. 2, 24 pigs (initial weight = 50.23 kg) were allotted to the 3 treatments using a replicated 3 × 3 Latin square design with 6 chambers (i.e., 4 pigs per chamber) and three 15-d periods. Oxygen consumption and CO2 and CH4 productions were measured during fed and fasting states and fecal and urine samples were collected. In both experiments, pigs were allowed ad libitum access to feed and water. Results indicated that there were no differences among watering options for SID of AA, feed intake, total and fasting heat production, ATTD of DM and GE, or NE in diet. In conclusion, different watering options did not affect the digestibility of AA, DM, and GE and concentration of NE in a corn-soybean meal diet fed to growing pigs.

Influence of age and type of feed ingredients on apparent and standardized ileal amino acid digestibility in broiler chickens.

Two experiments were conducted to determine the effects of bird age on apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) for 10-d-old Experiment (Exp. 1) and 22-d-old (Exp. 2) male broilers. This study investigated the effects of different broiler ages and feed ingredients on AID and SID of AA in corn and soybean meal (SBM). Four hundred and eighty (age = 7 d; initial body weight [BW] = 173.4 ± 12.65 g) and 192 (age = 18 d; initial BW = 772.2 ± 62.13 g) birds were allocated to three dietary treatments in a randomized complete block design with eight replicate cages per treatment. Two diets were formulated based on corn or SBM as the sole source of AA in the diet. A nitrogen-free diet was also formulated to measure basal endogenous losses of AA. Experimental diets were given for 3 and 4 days in Exps. 1 and 2, respectively. An interaction was observed (p < 0.05) between the age of birds and the type of ingredient for the AID of most AA, except for methionine, valine, cysteine (Cys), and tyrosine; however, the effects of age and type of ingredients were diminished in the SID of AA, except for histidine, isoleucine, leucine (Leu), phenylalanine, alanine (Ala), and glutamic acid (Glu). The AID of AA, except for Leu and Cys and the SID of AA, except for Leu, Ala, Glu, and Pro in SBM were greater (p < 0.05) than in corn. As the age of birds increased from 10 to 22 d, digestibility of all AA increased (p < 0.05), regardless of the expression of AA digestibility (i.e., AID and SID). In conclusion, the AID and SID of AA in both corn and SBM increased with increasing age, and the AID and SID of AA in SBM were greater than in corn.

Metabolizable energy and standardized ileal amino acid digestibility of full-fat soybeans for broilers are influenced by wet-heating, expansion temperature, and autoclaving time

The influence of wet-heating (WH) and expansion temperature (ET), and autoclaving time (AT) on the nitrogen-corrected apparent metabolizable energy (AMEn) and standardized ileal digestibility (SID) of AA in full-fat soybeans (FFSB) for broilers was examined in 2 experiments. The AMEn and SID AA of FFSB were determined by the difference and direct methods, respectively. In Experiment 1, raw FFSB (K0) were either treated by WH at 80°C for 1 min and expanded at 115°C (K1-115) or 125°C (K1-125), WH at 100°C for 6 min and expanded at 115°C (K2-115) or 125°C (K2-125), or WH at 100°C for 16 min and expanded at 115°C (K3-115) or 125°C (K3-125). Wet-heating and ET significantly (P < 0.001) increased the AMEn in FFSB. Among heat-treated FFSB, K1-115 and K1-125 resulted in the lowest and highest AMEn values, respectively, with all samples wet-heated at 100°C being intermediate. The K3-125 had AMEn values similar (P > 0.05) to K1-125. Among heat-treated FFSB, the highest average SID AA was recorded for K3-125. In Experiment 2, K3-125 from experiment 1 was divided into 9 batches and autoclaved at 110°C for 15 (Z1), 30 (Z2), 45 (Z3), 60 (Z4), 120 (Z5), 180 (Z6), 240 (Z7), 300 (Z8), and 360 (Z9) min. A quadratic (P < 0.01) pattern was observed for the effects of AT on AMEn. The AMEn was unaffected until 300 min AT and then declined at 360 min. The AT quadratically (P < 0.001) affected the average SID AA where the SID increased from K3-125 to Z1, plateaued to Z5, and then declined to Z9. In conclusion, the results demonstrated that WH at 100°C for 16 min followed by expansion at 125°C as the most optimal wet-heating and expansion processing, associated with the highest SID AA. Autoclaving at 110°C for 30 min enhanced energy utilization and AA digestibility in FFSB, suggesting that further advantages may be achieved by short-time autoclaving of previously wet-heated and expanded FFSB samples.

P70. Apparent and standardized ileal amino acid digestibilities in heat stressed pigs fed wheat-soybean meal diets supplemented with L-arginine and DL-methionine

P70. Apparent and standardized ileal amino acid digestibilities in heat stressed pigs fed wheat-soybean meal diets supplemented with L-arginine and DL-methionine

Corn distiller's dried grains with solubles as an alternative ingredient to corn and soybean meal in Pekin duck diets based on its predicted AME and the evaluated standardized ileal digestibility of amino acids

The study aimed to investigate the effects of dietary distillers dried grains with solubles (DDGS) levels on growth performance, carcass characteristic, serum biochemical indexes, meat physical and chemical quality, nutrient utilization, and standardized ileal digestibility of amino acids (SIDAA) in Pekin ducks aged 11 to 42 d based on the evaluation of its SIDAA. A total of 560 eleven-day-old Cherry Valley ducks were randomly allotted to 5 treatments with 7 replicate pens per treatment and 16 ducks per pen based on the average body weight. Six isonitrogenous and isocaloric experimental diets were formulated on a digestible amino acid basis to produce diets containing 0, 5, 10, 15, and 20% DDGS, respectively. With increasing of dietary DDGS levels, a linear and quadratic reduction (P < 0.05) was observed in the body weight (BW) at d 42, average day gain (ADG) and average day feed intake (ADFI) from d 11 to 42, breast meat yield, the moisture and protein content in the breast meat, and dietary DM and EE utilization. Moreover, a linear and quadratic increase (P < 0.05) was observed in the b* value of the breast meat and serum total cholesterol and triglyceride concentrations. Compared with the control group, the group with 10% inclusion of DDGS exhibited no adverse effect on growth performance, carcass characteristics, serum biochemical indexes, meat physical and chemical quality, nutrient utilization, and the SIDAA of the diets (P > 0.05). These results suggested that 10% of corn DDGS can function as an alternative ingredient to corn and soybean meal, and the optimal levels of DDGS in the diets of ducks aged from 11 to 42 d depends more on its quality.

Influence of Conditioning and Expansion Characteristics on the Apparent Metabolizable Energy and Standardized Ileal Amino Acid Digestibility of Full-Fat Soybeans for Broilers.

Simple SummaryFull-fat soybean (FFSB) is a valuable source of energy and protein for poultry and is obtained by cracking raw soybeans without cooking or oil extraction. However, due to the presence of trypsin inhibitors and lectins, raw FFSB is not used in poultry diets. These anti-nutritional factors are, however, heat-labile, and a proper hydrothermal process will inactivate these factors, eliminating their adverse effects on nutrient utilization and bird performance. The aim of the present study was to investigate the influence of conditioning and expansion characteristics on the apparent metabolizable energy and standardized ileal digestibility (SID) of amino acids (AA) in FFSB for broilers. Based on the results, long-term conditioning at 100 °C for 6 min followed by expansion with 18 kWh/t specific energy input increased the metabolizable energy and SID AA of FFSB. Further increases in conditioning time above 6 min or expansion of specific energy input above 18 kWh/t did not offer any advantages to metabolizable energy and AA digestibility of FFSB.This study investigated the influence of short-term and long-term conditioning and expansion on the nitrogen-corrected apparent metabolizable energy (AMEn) and standardized ileal digestibility (SID) of amino acids (AA) in full-fat soybeans (FFSB) for broilers. A batch of raw soybeans was used to manufacture 10 FFSB products (T0 to T9) by applying various combinations of conditioning and expansion. The AMEn and SID AA of FFSB were determined by difference and direct methods, respectively. All heat treatments increased (p < 0.001) the AMEn compared to raw FFSB. The sample subjected to long-term conditioning at 100 °C for 6 min and expansion at 18 kWh/t (T5) supported 3.88 MJ/kg higher AMEn than the raw FFSB. Raw FFSB had the poorest (p < 0.05) AA digestibility. Among the heat-treated samples, the highest (p < 0.05) SID AA was recorded for T5. The results demonstrated that the long-term conditioning of FFSB at 100 °C for 6 min prior to expansion with 18 kWh/t specific energy input enhanced metabolizable energy and SID AA. Further increases in conditioning time from 6 to 9 min or expansion of specific energy input from 18 to 28 kWh/t did not yield additional benefits to energy utilization and AA digestibility of FFSB.