Standard Typing Methods Research Articles

Correlation of tissue typing, mixed lymphocyte culture, and related donor renal allograft survival.

Results of mixed lymphocyte culture reactions and tissue typing were correlated with the clinical courses of recipients of living related donor renal allografts. Forty-nine patients tested by the mixed lymphocyte culture technique were divided into two response groups: stimulation index greater than 5 and stimulation index less than 5. Seventy patients were tested by standard tissue typing methods and were categorized by the number of misstimulation in mixed lymphocyte culture than with mismatched antigens, suggesting that lymphocyte-defined histocompatibility is more important than serologically defined histocompatibility in selecting the best possible allograft donor.

The Evaluation of a Rapid Card Technic for ABO and Rho(D) Typing

A simple card technic using seven drops of finger blood for rapid ABO and Rho(D) typing was compared with the standard typing methods of the JHH Blood Bank laboratory. When performed according to instructions, the card test showed distinct, easily readable reactions that were in excellent agreement with the results obtained by the standard method. Therefore, where Rh sub‐grouping is not required, or is to be carried out subsequently on all “Rho(D) negative” subjects, this card technic provides a useful, simple procedure suitable for field studies and all types of mass screening, since venipunctures are unnecessary, classification is rapid, and a permanent record available.