AbstractBlue grama (Bouteloua gracilis (H.B.K.) Lag. ex Steud.) seedlings often expand leaf area to the maximum that can be supported by water from the seminal root and then succumb to an increase in transpirational stress. The objective of this study was to define drought resistance of seedlings in relation to stages of shoot and root development. The changes in drought resistance that occur because of changes in seedling morphology have not been considered in previous quantifications of drought resistance. This information was essential for solving problems of seedling establishment on the shortgrass plains.Seedlings restricted to the seminal root, one adventitious root, or two adventitious roots were grown in moist soil in a controlled environment chamber, in the greenhouse, and in the field to further define seedling‐environment relationships. Relationships between drought of the shoot environment, maximum rate of water uptake, and maximum leaf area were investigated. Evaporation rate from a standard pan was used as a measurement of the drought of the shoot environment.Growth drought resistance, defined as the drought of the shoot environment that is just sufficient to halt expansion of seedling leaf area, was directly related to the critical evaporation/transpiration ratio for seedling growth and to the maximum rate of water uptake by the root; it was inversely related to seedling leaf area. In an environment with a constant evaporation rate, leaf area of seedlings had expanded to the limit of water supply when evaporation from a free water surface had exceeded transpiration rate six‐fold. Since growth drought resistance can be measured for individual seedlings, it will be useful in screening species and cultivars for seedlings with superior drought resistance, as well as for defining planting requirements.Survival drought resistance, defined as the drought of the shoot environment that is just sufficient to cause 50% death of seedlings, has only limited usefulness because of practical problems in its measurement. Large numbers of seedlings and a wide range of conditions are needed to accurately determine the relationship between drought and seedling death.