Recently, there has been an increase in publications devoted to sentinel lymph node biopsy (SLNB) in oral cancer. It has been established that cervical lymph node involvement, even in small primary tumors, is an unfavorable prognostic sign and is associated with a more than twofold decrease in overall survival. This creates a need to develop new methods for detecting hidden regional metastases, which, in turn, can improve long-term treatment outcomes. Our study included 16 patients with primary tongue cancer cT1-2N0M0 subject to selective or modified radical neck dissection, as well as radical tongue resection. Patients under 18 years of age, with previous neck surgery or radiation therapy, and with iodine allergy were excluded. Sentinel lymph node (SN) mapping was performed using the SPY-PHI Stryker navigation system, which simultaneously displays color video and fluorescence images. Tracers such as ICG (5 mg/mL) and methylene blue (10 mg/mL) were injected around the tumor, followed by a standard neck incision and measurement of fluorescence signal to identify SNs, which were then removed for histologic examination. All patients were able to reconstruct the tongue with local tissues and there were no technique-related complications. Sentinel lymph nodes (SLNs) were successfully identified in all cases, with four patients having histologically confirmed occult metastases. Nodal classification changed in three patients from cN0 to pN1 and one to pN2, and additional blue-staining lymph nodes were found in 11 patients, all of whom remained N0. The ICG sentinel lymph node method has been used in medicine since 1957 to assess organ function and angiography. ICG quickly binds to plasma proteins and is removed by the liver, making it a safe, radiation-free indicator that is less expensive than X-ray methods. The SNB procedure with ICG NIF imaging is simple and fast, but tissue swelling may occur during the first 24 hours. However, detection of nodes through the skin is difficult in patients with normal or high body weight, since the lymphatic vessels are located deeper and are blocked by surrounding tissue. A sentinel lymph node indicates damage to a specific lymphatic channel or collector.
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