As timber structures evolve towards greater heights and longer spans for buildings, there is a pressing need to enhance the fire resistance of timber structures. Considering the charring behavior of timber is crucial to the fire resistance of timber structures, it is a promising way to retard the charring behavior of timber and further enhance the fire resistance by applying transparent fireproof coatings. In this study, a series of fire tests were conducted to investigate the effect of transparent fireproof coatings on the charring behavior of Douglas fir glued laminated timber (Glulam) under small-scale ISO 834 standard fire conditions. Different coating types, coating quantities, exposed time to fire and member sizes were considered in the experiments. The experimental results including temperature profiles, maximum temperature, and charring rates for uncoated and coated Glulam specimens were carefully recorded and analyzed. Then, the effect of transparent fireproof coatings was quantified by the proposed ratio of delayed charring. The main mechanism of the delayed charring in fire was finally discussed. This study demonstrated that two transparent fireproof coatings can both effectively lower the temperature at different depths and delay the charring behavior of timber. The reduction in charring rates increased with the increase of coating quantity and decreased as the fire exposure time progressed. The ratio of delayed charring for two coatings can be over 30% in the initial stage of fire and over 20% for one-hour standard fire exposure. This study can provide technical support for enhancing the fire resistance of timber structures using transparent coatings.