Standard Diet Research Articles

Enteric glial NLRP3 inflammasome contributes to gut mucosal barrier alterations in a mouse model of diet-induced obesity.

In the present study, we investigated the involvement of NLRP3 inflammasome in the intestinal epithelial barrier (IEB) changes associated with obesity, and its role in the interplay between enteric glia and intestinal epithelial cells (IECs). Wild-type C57BL/6J and NLRP3-KO (-/-) mice were fed with high-fat diet (HFD) or standard diet for 8 weeks. Colonic IEB integrity and inflammasome activation were assessed. Immunolocalization of colonic mucosal GFAP- and NLRP3-positive cells along with invitro coculture experiments with enteric glial cells (EGCs) and IECs allowed to investigate the potential link between altered IEB, enteric gliosis, and NLRP3 activation. HFD mice showed increased body weight, altered IEB integrity, increased GFAP-positive glial cells, and NLRP3 inflammasome hyperactivation. HFD-NLRP3-/- mice showed a lower increase in body weight, an improvement in IEB integrity and an absence of enteric gliosis. Coculture experiments showed that palmitate and lipopolysaccharide contribute to IEB damage and promote enteric gliosis with consequent hyperactivation of enteric glial NLRP3/caspase-1/IL-1β signaling. Enteric glial-derived IL-1β release exacerbates the IEB alterations. Such an effect was abrogated upon incubation with anakinra (IL-1β receptor antagonist) and with conditioned medium derived from silenced-NLRP3 glial cells. HFD intake elicits mucosal enteric gliotic processes characterized by a hyperactivation of NLRP3/caspase-1/IL-1β signaling pathway, that contributes to further exacerbate the disruption of intestinal mucosal barrier integrity. However, we cannot rule out the contribution of NLRP3 inflammasome activation from other cells, such as immune cells, in IEB alterations associated with obesity. Overall, our results suggest that enteric glial NLRP3 inflammasome might represent an interesting molecular target for the development of novel pharmacological approaches aimed at managing the enteric inflammation and intestinal mucosal dysfunctions associated with obesity.

PSLBI-20 Dynamic response of piglets fed different sources and levels of fiber during an enterotoxigenic Escherichia coli (ETEC) challenge in the post-weaning transition

Abstract Dietary fiber can stimulate the growth of volatile fatty acid-producing bacteria, hence promoting gut health. A stimbiotic can accelerate the settlement of a fiber-fermenting microbiome after weaning. The present study aimed to investigate how different fiber types and a stimbiotic can support microbiome function and resilience of piglets facing an F18 ETEC challenge. Newly weaned piglets (n = 216) were blocked by body weight (BW) and randomly assigned to 1 of 4 dietary treatments (n = 9), including a corn-soy and wheat middlings control diet (CTL), high fiber diet (HF), CTL plus 0.01% stimbiotic (CS; Signis; AB Vista, UK), and HF plus 0.01% stimbiotic (HFS). The HF diets included sugar beet pulp at 5.0%, 3.8%, 2.5% and soy hulls at 4.0%, 3.0% and 2.0% for phases 1 (d 0 to 9), 2 (d 0 to 16), and 3 (d 16 to 22), respectively. A standard diet was fed during phase 4 (d 22 to 41). On d 5 post-weaning, all pigs were orally inoculated with 10 mL of F18 ETEC (108 CFU/mL). Twelve piglets were selected from different pens, and a fecal sample was collected on d 5 before challenge (0 d post-inoculation; dpi), d 12 (dpi 7) and d 29 (dpi 24) for analysis of microbial populations by qPCR and fecal calprotectin. Statistical analyses consisted of a multivariate analysis by age, with fiber, stimbiotic, and their interaction as fixed effects. There was no main effect of fiber or stimbiotic on final BW, and d 0 to 40 average daily gain (ADG) or gain to feed (G:F; P > 0.10). Overall, pigs fed CS had a greater intake than HFS (P < 0.05) with CTL and HF intermediate. Piglets fed CS and HF tended to have greater ADG from d 0 to 22 compared with pigs fed CTL (P < 0.10). Piglets fed CS tended to have heavier d 22 BW than CTL (P < 0.10), with HF and HFS intermediate. On dpi 0, butyrate-producing bacteria Lachnospiraceae and Ruminococcaceae were increased in piglets fed a stimbiotic (P < 0.05). On dpi 0 and 7, piglets fed HF had increased abundance of Lachnospiraceae (P < 0.05), whereas no difference was observed on dpi 24 (P > 0.10). Total E. coli did not vary between groups (P > 0.10). On dpi 0, calprotectin concentration was similar between treatments (averaged 25 ng/g feces; P > 0.10) Calprotectin was less in piglets fed a stimbiotic, at 7 vs 22 ng/g feces on dpi 7, and 8 vs 19 ng/g feces on dpi 24 in the stimbiotic and no stimbiotic group, respectively (P < 0.05). There was no fiber or fiber × stimbiotic interaction for calprotectin (P > 0.10). These results demonstrate that feeding high fiber may accelerate the settlement of the piglet microbiome profile as early as 5 d post-weaning. Feeding a stimbiotic may support the resilience of piglets facing an F18 ETEC challenge by promoting the growth of butyrate-producing bacteria, which alleviates the gastrointestinal tract inflammation response and ultimately boost growth performance.

Dietary supplementation with a blend composed of carvacrol, tannic acid derived from Castanea sativa and Glycyrrhiza glabra, and glycerides of medium chain fatty acids for weanling piglets raised in commercial farm.

This study aimed to evaluate the dietary administration of a blend composed of carvacrol, tannic acid derived from Castanea sativa mill and Glycyrrhiza glabra, medium chain fatty acids (MCFAs) glycerides for weanling piglets. An in vitro digestion followed by total phenolic content (TPC) and total antioxidant activity (TAC) assessment was performed before the in vivo application. At weaning, a total of 210 piglets were randomly allocated to two experimental treatments (7 replicates/15 piglets for each replicate). Control group (CTR) was fed a standard basal diet while the treated group (T) was fed the basal diet mixed with 1.500mg/kg of blend. After in vitro digestion, TPC and TAC evidenced peaks at the end of oral and gastric phases in comparison to the intestinal one in line with the high content of phenolic compound (P < 0.05). Treatment conditioned body weight and average daily gain (P < 0.05), fecal score on 6, 7, and 8 d after weaning (P < 0.05). At 35d, the T group showed a decrease in salivary cortisol compared to CTR (P < 0.05). Duodenum and jejunum sections of T piglets revealed higher villi (P < 0.05), deeper crypts (P < 0.01), and increased V/C ratio (P < 0.01). CTR showed a higher expression of duodenal Occludin (P < 0.05). Jejunal E-cadherin and Occludin were more expressed in T jejunum sections (P < 0.05). Twelve differentially abundant genera were identified in T group caecal samples. Potentially harmful Clostridium sensu stricto 13 was reduced by the treatment (P < 0.05). In conclusion, the tested blend positively affected salivary stress markers and the gut health of weaned piglets.

490 Lifetime growth performance and carcass composition of offspring when inactivated Saccharomyces cerevisiae was included in gestating and lactating gilt diets and/or offspring nursery diets

Abstract Gilts [n = 90; initial body weight (BW) = 189.1 ± 3.9 kg] were used to determine the effect of feeding a yeast additive (Saccharomyces cerevisiae inactivated through hydrolyzation; HY) to gestating and lactating gilts and/or the offspring during the nursery period on lifetime offspring growth performance. Gilts were randomly assigned to one of three dietary treatments (n = 30/treatment): standard gestation and lactation diets (GHY0) or supplemented with 0.25% (GHY0.25) or 1% (GHY1.0) HY. Diets were fed between d 85 of gestation until weaning at d 21 (2.6 kg/d in gestation, ad libitum in lactation). At weaning, 8 piglets/litter were placed in nursery pens and pens were randomly assigned to a control (NO) or a HY-supplemented diet (NHY), apart from GHY1.0 offspring who were only provided NO during the nursery period (n = 12). In nursery phases 1, 2 and 3, HY was included at 0.75, 0.50, and 0.25% respectively. Thereafter, pigs received standard grower and finisher rations until slaughter (160 d of age). Data were analyzed in SAS using GLIMMIX with gilt treatment as the main effect (pre-weaning); post-weaning performance used gilt and nursery treatments as the main effects, and interactive effects were tested separately due to the incomplete 3 × 2 factorial design. Mean comparisons were conducted using the Tukey-Kramer test to separate LSmeans. Gilt BW was unaffected by gilt treatment, but gilts fed GHY1.0 had greater total lactation feed intake versus GHY0.25 (128.6 vs. 109.4 ± 7.7 kg; P &amp;lt; 0.01). At birth, GHY0.25 offspring were 70 g heavier than pigs from GHY0 (P &amp;lt; 0.001) with GHY1.0 intermediate. At weaning, GHY0.25 and GHY1.0 offspring were heavier than GHY0 offspring (5.9 vs. 5.6 kg; P &amp;lt; 0.001). After weaning, neither gilt nor nursery diet influenced offspring BW. Pigs fed GHY1.0-NO had greater ADFI versus all other treatments in the nursery (interaction; P &amp;lt; 0.001), GHY0.25-NHY had greater ADFI than GHY0.25-NO and GHY1.0-NO in the grower (interaction; P = 0.005), and NHY consumed more feed than NO in the finisher phase (main effect; P &amp;lt; 0.001). In the nursery, NHY had less average daily feed intake (ADFI) than NO (main effect; P &amp;lt; 0.05), but in grower and finisher, NHY had greater ADFI than NO pigs (main effect; P &amp;lt; 0.001). In the nursery, gain to feed (G:F) was greater for GHY0-NHY versus GHY0.25-NHY and GHY1.0-NO, and greater for GHY0-NO versus GHY0.25-NHY with all other treatments intermediate (interaction; P &amp;lt; 0.05). In the grower phase, G:F was unaffected by maternal or nursery diet, but in the finisher phase, G:F was less for pigs fed NHY versus NO during the nursery phase (main effect; P &amp;lt; 0.05). Slaughter BW, hot carcass weight, and fat and lean percents were not influenced by gilt or nursery diet. Therefore, HY provided to gilts during gestation and lactation, improved pre-weaning offspring growth, but HY supplemented to pigs during the nursery period had greater positive effects on lifetime growth performance.

236 Increased dietary protein supports key metabolic and intestinal tissue functions in weaned pigs, without increasing gastrointestinal pathogen abundance

Abstract A common dietary strategy to prevent diarrhea in weaned pigs is feeding low protein diets in the period immediately following weaning. The goal of this intervention is to decrease dietary protein sufficiently to reduce pathogen proliferation and protein fermentation without negatively impacting growth performance. One potential alternative to low protein diets is provision of dietary fiber. However, many studies examining the role of protein and fiber on gastrointestinal microbiota and post-weaning diarrhea are complicated by the presence of other substrates, including polyphenols and antinutritional factors in complex ingredients. To assess the role of increased protein and fiber on gut microbiota and host metabolism, semi-purified diets differing in crude protein (CP) and crude fiber (CF) were fed to weaned pigs (n = 40; 28 d of age). Diets with high protein (HP; CP 24.3%, CF 6.4%), high fiber (HF; CP 18.9%, CF 9.4%), or both (HFHP; CP 24.9%, CF 9.5%) were compared with a control (CON; CP 18.1%, CF 6.3%) diet with industry standard crude protein and fiber levels (n = 10/group). Growth performance and diarrhea prevalence were measured alongside gut microbiota composition and metabolites. Pigs fed the HP and HFHP diets had significantly improved feed efficiency compared with both CON and HF diets. Increased protein fermentation, measured through production of biogenic amines, was observed in HP fed animals (P &amp;lt; 0.001). These effects were mitigated by addition of fiber in the HFHP fed pigs. Despite increased markers of protein fermentation, post-weaning diarrhea incidence was not greater in HP fed animals and no increases in gastrointestinal pathogen abundance were detected. Metabolomic and transcriptomic analyses identified critical alterations in host metabolism and cecal transcriptome in the CON compared with the HP fed animals. While all diets were formulated to meet the dietary requirements of weaned pigs, diets with lower protein levels (CON and HF) induced alteration in transcripts from the serine synthesis pathways (P &amp;lt; 0.001) and integrated stress response (P &amp;lt; 0.001) in cecal tissue alongside increases in metabolic pathways related to lysine degradation (P = 0.005). These results challenge the current practice of low protein feeding following weaning by demonstrating a detrimental effect of low protein diets on intestinal cell function and muscle accretion. This suggests that with careful ingredient selection, increased dietary protein post-weaning could better support pig health and performance compared with current industry standard diets.

484 Exploring fear in client-owned dogs: Insights from behavioral testing, owner surveys, and biomarker validation

Abstract Since the SARS-CoV-2 pandemic, pet professionals and owners have observed an increase in anxiety-related behaviors, including fearfulness, contributing to behavioral problems, reduction in pet and owner welfare, and increased rates of shelter relinquishment. The objectives of this study were to evaluate canine demographics and owner-identified fearfulness compared with fear-related behaviors during in-person behavior tests, with further verification using fear-related biomarkers. Client-owned dogs [n = 102; 25.3 ± 11.6 kg body weight (BW)] were included between 1 to 10 yr of age, of varying breeds, sex and neuter statuses, and home dynamics. Dogs had no known health issues, were not receiving medication, and were fed a standardized diet for 4 wk prior to testing. Owners provided information via a questionnaire regarding fear, anxiety, excitability, and separation behaviors, as well as information on the lifestyle and background of the dog. Behavior tests, which consisted of a novel human, novel object, and an open field test, were performed indoors in a constructed 3 x 3 m arena at the University of Guelph. Fecal samples were provided by owners and blood samples were collected immediately following the behavior assessment. Commercial ELISA kits were used to quantify fecal IgA, serum serotonin (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA), then the 5-HIAA:5-HT ratio was calculated. Behavior tests were recorded on a GoPro camera, and behaviors were coded using BORIS. For preliminary analysis, owner questionnaire data relating to human-directed fear was separated into frequency of human-directed fear (HFF), intensity of human-directed fear (HFI), frequency of fear-related behaviors during grooming or veterinary visits (VHF), and intensity of fear-related behaviors during grooming or veterinary visits (VHI) section. Regression analyses were conducted using SAS, with outcomes derived from behavioral tests serving as dependent variables, and predictor variables encompassing sex, neuter status, age, BW, biomarker concentrations, and owner survey scores. Significance was determined at P ≤ 0.05 and trends at P ≤ 0.10. Preliminary data analysis using data from the novel human test of 50 dogs indicates that the duration of reduced body posture during the test was positively correlated with VHF (P = 0.0201) and VHI (P = 0.0226) scores, and negatively correlated with HIAA concentrations (P = 0.0134). The duration of the “side” ear position was also positively associated with HFF (P = 0.0219), HFI (P = 0.0471), and VHI (P = 0.0170) scores, with a trend observed in VHF (P = 0.0818) scores. Duration of time with ears pinned back was similarly associated with HFF (P = 0.0064), HFI (P = 0.0076), and VHI (0.0192) scores. These results suggest that behavior may be reliably predicted from owner-completed surveys and can be a valuable tool in gathering information for both professionals and the public. Further data analysis from the present study will be conducted to further understand the relationship between canine demographics, owner identified fear scores, physiological biomarkers and behavior testing results.

295 Synthesis of glycine from trans-4-hydroxy-L-proline in tissues of juvenile hybrid striped bass (Morone chrysops x M. Saxatilis)

Abstract Trans-4-Hydroxy-L-Proline (Hyp) is a major amino acid in the collagen of fish. The turnover of collagen in various tissues releases a large amount of Hyp, which has traditionally been considered as a metabolic waste. However, results of recent studies have identified the conversion of Hyp into glycine in piglets. The present study tests this hypothesis that hybrid striped bass (HSB, a carnivorous fish species) can form glycine from Hyp. Fish were fed a standard diet containing 60% fishmeal for 10 wk and grew from 5.5 to 45 g during a 9-wk period. Slices (~75 mg) of skeletal muscle, liver, proximal intestine, gills, and brain were obtained from 45-g HSB (n = 6) and incubated at 26 °C for 2 h in 1 mL of oxygenated (95% O2/5% CO2) Krebs-Henseleit bicarbonate buffer (pH 7.4, with 5 mM D-glucose) containing 0, 0.2, or 2 mM Hyp. After a period of 2 h incubation, 200 μL of 1.5 M HClO4 was added into the incubation medium to stop the reaction. The acidified medium plus tissue together was homogenized and the homogenizer was rinsed with 1 mL of water, followed by the addition of 100 μL of 2 M K2CO3. The neutralized extract was analyzed for amino acids using HPLC involving o-phthaldialdehyde derivatization. Tissues without incubation were also determined for amino acids. Data were statistically analyzed by a paired t-test. Glycine was produced from 0.2 and 2 mM Hyp by the liver and skeletal muscle, and 2 mM Hyp by the intestine (Table 1). Glycine was further converted into serine in the liver, but not skeletal muscle or intestine. The production of glycine or serine from Hyp was not due to net protein breakdown in tissues. Tyrosine was measured as an indicator of protein degradation. There was no detectable generation of glycine from 0.2 or 2 mM Hyp by the gills or brain of HSB. These results indicate the synthesis of glycine from Hyp by juvenile HSB in a tissue-specific manner. Because skeletal muscle accounts for ~45% of the body weight, this organ is the major site for this metabolic pathway in the fish. (Supported by USDA/NIFA grant No. 2022-67015-36200)

Synbiotic supplementation ameliorates anxiety and myocardial ischaemia-reperfusion injury in hyperglycaemic rats by modulating gut microbiota.

Hyperglycaemia, hyperlipidaemia, hypertension and obesity are the main risk factors affecting the development and prognosis of ischaemic heart disease, which is still an important cause of death today. In our study, male Sprague-Dawley rats were fed either a standard diet (SD) or a high fat and high carbohydrate diet (HF-HCD) for 8weeks and streptozotocin (STZ) was injected at the seventh week of the feeding period. In one set of rats, a mixture of a prebiotic and probiotics (synbiotic, SYN) was administered by gavage starting from the beginning of the feeding period. Experimental myocardial ischaemia-reperfusion (30min/60min) was induced at the end of 8weeks. Hyperglycaemia, hypertension and increased serum low-density lipoprotein levels occurred in SD- and HF-HCD-fed and STZ-treated rats followed for 8weeks. Increased density of the Proteobacteria phylum was observed in rats with increased blood glucose levels, indicating intestinal dysbiosis. The severity of cardiac damage was highest in the dysbiotic HF-HCD-fed hyperglycaemic rats, which was evident with increased serum creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), tumour necrosis factor-α, and interleukin-6 levels, along with a decrease in ST-segment resolution index. SYN supplementation to either a normal or a high-fat high-carbohydrate diet improved gut dysbiosis, reduced anxiety, decreased CK-MB and cTnI levels, and alleviated myocardial ischaemia-reperfusion injury in hyperglycaemic rats.

P-Coumaric acid potential in restoring neuromotor function and oxidative balance through the Parkin pathway in a Parkinson disease-like model in Drosophila melanogaster

p-Coumaric acid is a significant phenolic compound known for its potent antioxidant activity. Thus, this study investigated the effects of p-coumaric acid on the behavioral and neurochemical changes induced in Drosophila melanogaster by exposure to rotenone in a Parkinson disease (PD)-like model. The flies were divided into four groups and maintained for seven days on different diets: a standard diet (control), a diet containing rotenone (500 μM), a control diet to which p-coumaric acid was added on the fourth day (0.3 μM), and a diet initially containing rotenone (500 μM) with p-coumaric acid added on the fourth day (0.3 μM). Exposure to p-coumaric acid ameliorated locomotor impairment and reduced mortality induced by rotenone. Moreover, p-coumaric acid normalized oxidative stress markers (ROS, TBARS, SOD, CAT, GST, and NPSH), mitigated oxidative damage, and reflected in the recovery of dopamine levels, AChE activity, and cellular viability post-rotenone exposure. Additionally, p-coumaric acid restored the immunoreactivity of Parkin and Nrf2. The results affirm that p-coumaric acid effectively mitigates PD-like model-induced damage, underscoring its antioxidant potency and potential neuroprotective effect.

Impact of Extremely Low-Frequency Electromagnetic Fields on Skeletal Muscle of Sedentary Adult Mice: A Pilot Study.

Extremely low-frequency electromagnetic fields (ELF-EMFs) are ubiquitous in industrialized environments due to the continuous use of electrical devices. Our previous studies demonstrated that ELF-EMFs affect muscle cells by modulating oxidative stress and enhancing myogenesis. This pilot study investigated these effects on the skeletal muscles of sedentary adult mice, assessing physiological responses to ELF-EMF exposure and potential modulation by antioxidant supplementation. Male C57BL/6 mice were exposed to ELF-EMFs (0.1 or 1.0 mT) for 1 h/day for up to 5 weeks and fed a standard diet without or with N-acetyl-cysteine (NAC). The results showed transient increases in muscle strength (after 2 weeks of exposure at 1.0 mT), potentially linked to muscle fiber recruitment and activation, revealed by higher PAX7 and myosin heavy chain (MyH) expression levels. After ELF-EMF exposure, oxidative status assessment revealed transient increases in the expression levels of SOD1 and catalase enzymes, in total antioxidant capacity, and in protein carbonyl levels, markers of oxidative damage. These effects were partially reduced by NAC. In conclusion, ELF-EMF exposure affects skeletal muscle physiology and NAC supplementation partially mitigates these effects, highlighting the complex interactions between ELF-EMFs and antioxidant pathways in vivo. Further investigations on ELF-EMFs as a therapeutic modality for muscle health are necessary.

Strategy for treating MAFLD: Electroacupuncture alleviates hepatic steatosis and fibrosis by enhancing AMPK mediated glycolipid metabolism and autophagy in T2DM rats

BackgroundRecent studies have highlighted type 2 diabetes (T2DM) as a significant risk factor for the development of metabolic dysfunction-associated fatty liver disease (MAFLD). This investigation aimed to assess electroacupuncture’s (EA) impact on liver morphology and function in T2DM rats, furnishing experimental substantiation for its potential to stall MAFLD progression in T2DM.MethodsT2DM rats were induced by a high-fat diet and a single intraperitoneal injection of streptozotocin, and then randomly assigned to five groups: the T2DM group, the electroacupuncture group, the metformin group, combination group of electroacupuncture and metformin, combination group of electroacupuncture and Compound C. The control group received a standard diet alongside intraperitoneal citric acid - sodium citrate solution injections. After a 6-week intervention, the effects of each group on fasting blood glucose, lipids, liver function, morphology, lipid droplet infiltration, and fibrosis were evaluated. Techniques including Western blotting, qPCR, immunohistochemistry, and immunofluorescence were employed to gauge the expression of key molecules in AMPK-associated glycolipid metabolism, insulin signaling, autophagy, and fibrosis pathways. Additionally, transmission electron microscopy facilitated the observation of liver autophagy, lipid droplets, and fibrosis.ResultsOur studies indicated that hyperglycemia, hyperlipidemia and IR promoted lipid accumulation, pathological and functional damage, and resulting in hepatic steatosis and fibrosis. Meanwhile, EA enhanced the activation of AMPK, which in turn improved glycolipid metabolism and autophagy through promoting the expression of PPARα/CPT1A and AMPK/mTOR pathway, inhibiting the expression of SREBP1c, PGC-1α/PCK2 and TGFβ1/Smad2/3 signaling pathway, ultimately exerting its effect on ameliorating hepatic steatosis and fibrosis in T2DM rats. The above effects of EA were consistent with metformin. The combination of EA and metformin had significant advantages in increasing hepatic AMPK expression, improving liver morphology, lipid droplet infiltration, fibrosis, and reducing serum ALT levels. In addition, the ameliorating effects of EA on the progression of MAFLD in T2DM rats were partly disrupted by Compound C, an inhibitor of AMPK.ConclusionsEA upregulated hepatic AMPK expression, curtailing gluconeogenesis and lipogenesis while boosting fatty acid oxidation and autophagy levels. Consequently, it mitigated blood glucose, lipids, and insulin resistance in T2DM rats, thus impeding liver steatosis and fibrosis progression and retarding MAFLD advancement.

Salt-sensitive hypertension in GR mutant rats is associated with altered plasma polyunsaturated fatty acid levels and aortic vascular reactivity.

In humans, glucocorticoid resistance is attributed to mutations in the glucocorticoid receptor (GR). Most of these mutations result in decreased ligand binding, transactivation, and/or translocation, albeit with normal protein abundances. However, there is no clear genotype‒phenotype relationship between the severity or age at disease presentation and the degree of functional loss of the receptor. Previously, we documented that a GR+/- rat line developed clinical features of glucocorticoid resistance, namely, hypercortisolemia, adrenal hyperplasia, and salt-sensitive hypertension. In this study, we analyzed the GR+/em4 rat model heterozygously mutant for the deletion of exon 3, which encompasses the second zinc finger, including the domains of DNA binding, dimerization, and nuclear localization signals. On a standard diet, mutant rats exhibited a trend toward increased corticosterone levels and a normal systolic blood pressure and heart rate but presented with adrenal hyperplasia. They exhibited increased adrenal soluble epoxide hydroxylase (sEH), favoring an increase in less active polyunsaturated fatty acids. Indeed, a significant increase in nonactive omega-3 and omega-6 polyunsaturated fatty acids, such as 5(6)-DiHETrE or 9(10)-DiHOME, was observed with advanced age (10 versus 5weeks old) and following a switch to a high-salt diet accompanied by salt-sensitive hypertension. In thoracic aortas, a reduced soluble epoxide hydrolase (sEH) protein abundance resulted in altered vascular reactivity upon a standard diet, which was blunted upon a high-salt diet. In conclusion, mutations in the GR affecting the ligand-binding domain as well as the dimerization domain resulted in deregulated GR signaling, favoring salt-sensitive hypertension in the absence of obvious mineralocorticoid excess.

Comparison of weight loss effects among overweight/obese adults: A network meta-analysis of mediterranean, low carbohydrate, and low-fat diets

Eating patterns significantly impact the weight loss process. This study aimed to investigate the influence of primary eating patterns on weight loss measures in overweight and obese adults using network meta-analysis. We systematically searched PubMed, Scopus, Web of Science (WOS), and Google Scholar until May 2, 2023. Our network meta-analysis followed the PRISMA extension guidelines for Comparing Mediterranean, low carbohydrate and low fat diet effects on weight loss among overweight/obese adults. We conducted a Frequentist random-effects network meta-analysis. Summary effects were presented as mean differences (MD) along with corresponding standard deviations (SD). P-scores were used for treatment ranking within the network. Initial literature searches yielded 1574 citations. Ultimately, 1004 participants from 7 RCTs (or 9 trials) met inclusion criteria. All diets resulted in weight loss. Comparatively, the low-carbohydrate diet exhibited a significant decrease in weight loss compared to the Mediterranean diet (MD=-2.70kg, 95% CI:-4.65,-0.75). Indirect evidence revealed that both the low-carbohydrate diet (MD=-6.31kg, 95% CI:-11.23,-1.39) and the low-fat diet (MD=-5.61kg, 95% CI:-10.61,-0.61) significantly reduced weight among overweight/obese adults compared to the standard hypolipemic diet. Rankings indicated the low-carbohydrate diet as the most effective dietary intervention for enhancing weight loss (P-score=0.8994) and reducing body fat (P-score=0.7060). Overall, a low-carb diet appears to be among the most effective approaches for weight loss and body fat reduction. However, it's essential to consider that its efficacy may vary based on factors such as age, gender, genetics, and lifestyle habits.

A Cellulose-Rich Diet Disrupts Gut Homeostasis and Leads to Anxiety through the Gut-Brain Axis.

It is widely said that a healthy intestinal environment plays an essential role in better mental condition. One known dietary nutrient that maintains the intestinal environment is dietary fiber. A recent study showed that maintaining the intestinal environment with dietary fiber alleviated symptoms of psychiatric disorders in animals. However, such effects have only been reported with soluble fiber, which is highly fermentable and promotes short-chain fatty acid (SCFA) production, and not with insoluble fiber. Therefore, we aimed to verify whether insoluble fiber, such as cellulose, can alter emotion via changes in the gut. We divided mice into two groups and fed either a standard diet (SD, which contains both insoluble and soluble dietary fibers) or a cellulose-rich diet (CRD, which contains cellulose alone as the dietary fibers). We found that CRD-fed mice display increased anxiety-like behavior. CRD-fed animals also showed decreased intestinal SCFA levels along with increased intestinal permeability, dysmotility, and hypersensitivity. This behavioral and physiological effect of CRD has been completely abolished in vagotomized mice, indicating the direct link between intestinal environment exacerbation to the emotion through the gut-brain axis. Additionally, we found that amygdalar dopamine signaling has been modified in CRD-fed animals, and the opioid antagonist abolished this dopaminergic modification as well as CRD-induced anxiety. Altogether, our findings indicate that consumption of cellulose alone as the dietary fiber may evoke intestinal abnormalities, which fire the vagus nerve, then the opioidergic system, and amygdalar dopamine upregulation, resulting in the enhancement of anxiety.

The effects of brown rice as functional food on Lee Index, adipose tissues and PRDM16 levels in obesity model &lt;i&gt;Rattus norvegicus&lt;/i&gt;

Brown rice is a functional food known to improve the Lee Index, influencing PRDM16 levels in obesity conditions. Therefore, this study aims to determine the differences in Lee Index, Brown Adipose Tissue (BAT) weight, White Adipose Tissue (WAT) weight, BeAT weight, total body fat, and PRDM16 levels of obese model rats with the intervention of brown and white rice, as well as ℽ-oryzanol. A true experimental method was used with a post-test-only control group in vivo design. The obesity model was constructed with male Sprague Dawley rats (Rattus norvegicus), divided into five diet groups namely standard and HFHF diet control, as well as HFHF + brown rice, HFHF + white rice, and HFHF + ℽ-oryzanol combination diet. The experiment was carried out for 26 weeks, with details of 14 weeks to form an obese model and 12 weeks for the intervention. Before statistical correlation was tested, Lee index values, adipose tissues, and PRDM16 were analyzed. The anthropometric data collection method was carried out by weighing before and after the intervention, while adipose tissue was collected by weighing after sacrifice. The immunofluorescence method was used to collect the expression of PRDM16 and the mean of PRDM16 levels was analyzed in the ImageJ application. After the data collection process, analysis was performed using SPSS to determine possible differences in each group. Normally distributed data were analyzed using One-Way ANOVA, while those without normal distribution were assessed using the Kruskall-Walis method and the Mann Whitney-U advanced test, with a p-value of &lt;0.05 considered significantly different.The result showed that there were differences among several groups regarding total body fat (p=0.012), WAT (p=0.026), and BAT (p=0.025). However, no differences were found between all groups regarding the Lee index (p=0.275), BeAT (p=0.079), and PRDM16 level (p=0.292). In conclusion, brown rice intervention did not significantly affect Lee index values, the expression of PRDM16, and adipose tissue weights at the end of the intervention.

Dietary patterns and periodontitis: A systematic review.

The systematic review aimed to investigate the associations between index-based dietary patterns and the risk and severity of periodontitis. Four public databases were searched for relevant published articles. Two independent researchers conducted the study selection, quality assessment, and data extraction. Methodological quality of the selected studies was evaluated using Joanna Briggs Institute Checklists. The review was registered with PROSPERO (CRD42023395049). Twenty-five studies were eligible for this review, including 23 cross-sectional studies and two prospective cohort studies. The most utilized dietary indices were the Healthy Eating Index (HEI), the Mediterranean Diet Score (MDS), and the Dietary Inflammatory Index (DII). The results indicated a positive association between higher diet quality (i.e., higher HEI and MDSs and lower DII scores) and healthier periodontal status. Subgroup meta-analysis for four studies utilizing HEI and CDC/AAP case definition indicates the protective effect of higher HEI scores on the risk of periodontitis (OR [95% CI] = 0.77[0.68, 0.88]) with statistical significance (Z = 3.91 [p < 0.0001]). Dietary assessment was conducted by validated food frequency questionnaires (FFQ) in 52% of the studies and 24-h dietary recalls in 36% of the studies. One study utilized a validated 15-item questionnaire to measure patients' adherence to the Mediterranean Diet (QueMD). The quality assessment showed that all studies were of high quality. High HEI and MDSs and low DII scores were associated with a low risk of periodontitis and better periodontal conditions. The standardized and repeatable diet guidelines might be provided for preventing periodontitis. Future prospective studies and clinical trials are needed to confirm this causal association.

The Effects of Fermented Cauliflower Residue Feed on the Diarrhea Rate, Intestinal Morphology, Immune Indicators, and Intestinal Flora of Weaned Piglets

This study investigated the effects of incorporating fermented feed into the post-weaning piglet diet, with a focus on its impact on the growth, health, serum markers, immune function, diarrhea rate, and microbial community of post-weaning piglets. Forty-two post-weaning piglets (Duroc × [Landrace × Yorkshire], castrated male), with good health and similar weight (11.40 ± 1.36 kg) at 40 days of age, were randomly assigned to two groups. Throughout the trial, one group received a standard diet (control group) while the other one was given a diet supplemented with fermented feed (experimental group), respectively. The findings revealed that compared to the control group, the post-weaning piglets fed with fermented feed showed increased daily weight gain. The fermented feed also led to an increase in the height of villi in the duodenum or jejunum of post-weaning piglets (p &lt; 0.05), accompanied by an improved ratio of villus height to crypt depth in the experimental group (p &lt; 0.05). Furthermore, the fermented feed enhanced the richness and diversity of the intestinal microbial community in post-weaning piglets (p &lt; 0.05), particularly increasing the relative abundance of Lactobacillaceae and Bifidobacteriaceae. These findings demonstrate that incorporating the fermented feed into the diet can reduce the diarrhea rate, improve growth performance, enhance immune function, and promote intestinal development in post-weaning piglets.

Paternal zinc deficiency alters offspring metabolic status in Drosophila melanogaster

BackgroundThis study delves into the understudied yet potentially crucial role of paternal zinc deficiency in programming offspring metabolic outcomes. By examining paternal zinc deficiency, we aim to shed light on a previously unexplored avenue with the potential to significantly impact future generations. We investigated the intergenerational effects of paternal zinc deficiency on metabolic parameters in Drosophila melanogaster. MethodsDietary zinc deficiency was induced by supplementing the diet of Drosophila F0 male flies with TPEN (N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine) from egg stage. The F0 male flies after eclosion were mated with age-matched virgin female flies from the control group, resulting in the F1 offspring generation. The F1 generation were then cultured on a standard diet for subsequent metabolic analyses, including assessments of body weight, locomotion, and levels of glucose, trehalose, glycogen, and triglycerides as well as the expression of related genes. ResultsWe observed an increase (p<0.05) in body weight in male parent flies and female offspring. Negative geotaxis performance was also impaired in the female offspring. Paternal zinc deficiency exerted distinct effects on carbohydrate and lipid metabolism, as evidenced by a significant (p<0.05) increase in trehalose and triglyceride levels in both parent and offspring. Additionally, zinc deficiency led to alterations in the expression of key metabolic genes, including significant (p<0.05) increase in DILP2 mRNA levels, highlighting potential links to insulin signaling. Also, there were reduced mRNA levels of SOD1 and CAT in both parental and offspring generations. Parental zinc deficiency also increased the expression of Eiger and UPD2 mRNA in the offspring, suggesting potential perturbations in the immune response system. ConclusionThese findings underscore the link between zinc status and various physiological and molecular processes, revealing both immediate and intergenerational impacts on metabolic, antioxidant, and inflammatory pathways and providing valuable insights on the implications of paternal zinc deficiency in Drosophila melanogaster.

Effects of lycopene on the growth performance, meat quality, cecal metagenome, and hepatic untargeted metabolome in heat stressed broilers

The occurrence of heat stress in poultry houses is inevitable and leads to oxidative stress in the birds. Lycopene, a natural hydrocarbon carotenoid, possesses potent antioxidant properties. This study aimed to investigate the impact of lycopene on growth performance, meat quality, cecal microflora, and liver metabolome in broilers subjected to heat stress. A total of 480 yellow feather broilers were randomly allocated into 4 treatment groups: birds fed standard diet (Con), birds fed standard diet and supplemented with lycopene (Lyc), birds fed standard diet and subjected to heat stress (Hs), and birds fed with lycopene and subjected to heat stress (Hs-Lyc). As compared with the normal temperature groups, Hs decreased the average daily gain (ADG) of birds during d 1 to 28, lowered the pH value either in breast meat or thigh meat, increased the L* value of breast meat, and decreased the a* value of thigh meat. In comparison with non-Lyc feeding birds, Lyc supplement elevated the ADG during d 1 to 56, increased the pH of breast meat, decrease the L* and b* values of thigh meat, simultaneously increase the a* value of thigh meat. The L* of breast meat and pH of thigh meat exhibited significant differences under Hs-Lyc treatment. Lyc-treated birds exhibited higher elasticity, gumminess, and resilience in breast meat than those in non-Lyc feeding birds. The cecal metagenome analysis indicated that Hs-Lyc treatment increased the abundance of Phocaeicola salanitronis and Prevotella sp.CAG:1058, Bacteroides sp.An269, and Bacteroides sp.An19 at the species level compared with other treatments. The hepatic untargeted metabolome analysis showed that administration of Lyc upregulated 20 metabolites and downregulated 60 metabolites compared to the Con birds. Futhermore, the Hs-Lyc treatment upregulated 34 metabolites and downregulated 45 metabolites compared to the Hs birds. The correlation between the metagenome and metabolome showed that Lyc supplementation induced significant alterations in the citrate cycle, metabolism of butanoate, glycolysis/gluconeogenesis, glyoxylate and dicarboxylate, alanine, aspartate, and glutamate compared with standard supplement. In contrast, Hs-Lyc treatment induced alterations in the citrate cycle, metabolism of pyruvate, glyoxylate, and dicarboxylate, glycolysis/gluconeogenesis, arginine, proline, alanine, aspartate, and glutamate compared with the standard supplement of heat-challenged broilers. In summary, dietary Lyc supplementation promoted the growth performance, changed the meat quality, modulated the cecal metagenome and hepatic metabolome in heat-stressed broilers.

Вплив комплексу біопротекторів на динаміку активності амінотрансфераз у сироватці крові за умов окремої та одночасної дії свинцю і фтору (експериментальні дослідження)

Lead and fluoride enter the human body individually and simultaneously through water, food, and air, disrupting metabolic processes in liver tissue. The sequential application of a bioprotective complex that reduce the negative effects of lead and fluoride has been insufficiently studied. The aim of the study is to investigate the changes over time in the activity of alanine aminotransferase and aspartate aminotransferase in the blood serum of laboratory animals under prolonged separate and simultaneous exposure to lead and fluoride, without application of bioprotective agents and during the sequential addition of pectin, pectin with calcium, and a complex of pectin, calcium, and antioxidants to the standard animal diet. Materials and Methods. Four clinical trial series were conducted on white rats, each following an orthogonal design 22. Aqueous solutions of Pb(NO3)2 and NaF were administered orally separately and simultaneously over 30 days: in the first series without bioprotective agents, in the second with pectin, in the third with pectin and calcium, and in the fourth with a complex of pectin, calcium, and antioxidants – C, E, and β-carotene vitamins, and selenium. The activity of alanine and aspartate aminotransferases was measured in blood serum on days 3, 15, and 30 in the first and second series of experiments, and on day 30 in the third and fourth series. Results. Separate and simultaneous oral administration of lead and fluoride to white rats leads to increased activity of alanine and aspartate aminotransferases in blood serum. The maximum changes were observed at the end of the experiment. The protective role of pectin alone under long-term fluoride expos ure and simultaneous exposure to lead and fluoride was not sufficiently effective. Concurrent intake of calcium and pectin significantly reduced the increase in transaminase activity, indicating an improvement in the metabolic and functional state of the liver. The application of the bioprotective complex restored the aminotransferase activity in blood serum. Conclusions. The complex of pectin with calcium and antioxidants can be considered an optimal means of correcting aminotransferase activity in blood serum under prolonged separate and simultaneous exposure to lead and fluoride.