Introduction: Menstrual blood stem cell banking allows women to store their menstrual blood under suitable conditions and preserve it for future use. Aim: This study aims to assess the effectiveness of a structured teaching program on the level of knowledge among staff nurses working at selected hospitals of the Valsad district. Methodology: Quantitative approach with a pre-experimental research design by one-group pre-test and post-test was used. A total of 100 staff nurses were selected for data collection. Self-structured questionnaires were used to assess level of knowledge and association with socio-demographic variables. After conducting a pre-test, a structured teaching program on menstrual blood stem cell banking was administered to the participants and post-test was conducted after a relapse of seven days. Results: Overall level of knowledge in Pre-test shows that 9% had average knowledge and 91% had poor knowledge and none had good knowledge. In Post-test, None of the Staff Nurse is had poor knowledge, 61% of had average knowledge and 39% had good knowledge regarding Menstrual blood stem cell banking. This present study shows the Mean of Pre-test was 6.36 and significantly improved to 17.42 in Post-test with the mean difference of 11.06. The standard deviation of Pre-test was 3.76 and Post-test was 2.4. The computed value of t= 25.04 which is greater than the table value of t= 1.645 at the level of p at 0.05. There was a significant association between the pre-test knowledge score of staff nurses and selected socio-demographic variables, including educational status and previous knowledge about menstrual blood stem cell banking by chi- square method. Conclusion: The study concluded that the structured teaching program is effective in improving the level of knowledge of staff nurses regarding menstrual blood stem cell banking.