Aging is often accompanied with the onset of sarcopenia, defined by low muscle mass, strength, and physical function. Regular resistance exercise may mitigate this loss; however, data are lacking that compare young and old adults who do and do not perform resistance training for general health on skeletal muscle mass and physical function. PURPOSE: The aim of this study was to identify differences in muscle mass and physical function between young and old adults who did and did not perform resistance training for general health. METHODS: Healthy younger (23.8 ± 0.6 yrs) and older (68.6 ± 1.7 yrs) men and women (n = 67) who either did or did not regularly participate in resistance training were assigned to 1 of 4 groups: young trained (YT: n = 22); young not trained (YNT: n = 15); old trained (OT: n = 11); and old not trained (ONT: n = 19). Dual energy x-ray absorptiometry assessed appendicular skeletal muscle mass (SMI). Participants performed 4 physical function tests: stair climb (SC), 30s sit-to-stand (30sSTS), 6-min walk test (6MWT), and timed-up-and-go (TUG). ANOVAs were used to compare groups for all measures, p ≤ 0.05. Data are reported as mean ± SE. RESULTS: We found differences between groups for SMI, SC, 30sSTS, 6MWT, and TUG (p ≤ 0.05). SMI was higher for YT compared to YNT (p = 0.003) and ONT (p > 0.003; YT: 8.60 ± 0.37 kg/m2, YNT: 6.88 ± 0.17 kg/m2, OT: 7.69 ± 0.42 kg/m2, ONT: 7.04 ± 0.29 kg/m2). SC performance was faster for YT (p < 0.0001), YNT (p < 0.0001) and OT (p = 0.03) compared to ONT (YT: 2.37 ± 0.05 s, YNT: 2.52 ± 0.08 s, OT: 2.81 ± 0.12 s, ONT: 3.33 ± 0.17 s). For 30sSTS, OT completed more reps than ONT (p < 0.0001) and YT completed more reps than YNT (p = 0.001) and ONT (p < 0.0001; YT: 22.8 ± 0.5 reps, YNT: 18.9 ± 0.7 reps, OT: 21.6 ± 1.3 reps, ONT: 16.7 ± 0.6 reps). For 6MWT, OT walked farther than ONT (p = 0.017) and YT walked farther than ONT (p = 0.001; YT: 837.0 ± 16.7 yds, YNT: 785.5 ± 15.1 yds, OT: 829.2 ± 31.9 yds, ONT: 733.6 ± 20.4 yds). For TUG, OT was faster than ONT (p = 0.006; YT: 5.81 ± 0.17 s, YNT: 5.88 ± 0.26 s, OT: 5.21 ± 0.23 s, ONT: 6.38 ± 0.23 s). SMI, SC, 30sSTS, 6MWT and TUG were not different between OT and YT or YNT (p > 0.05). All other comparisons were not significantly different (p > 0.05). CONCLUSION: These data suggest that older adults who engage in regular resistance training may maintain muscle mass and physical function similar to that of younger adults.