Stainless Steel Wire Electrodes Research Articles

An anesthesia protocol for robust and repeatable measurement of behavioral visceromotor responses to colorectal distension in mice.

Visceral motor responses (VMR) to graded colorectal distension (CRD) have been extensively implemented to assess the level of visceral pain in awake rodents, which are inevitably confounded by movement artifacts and cannot be conveniently implemented to assess invasive neuromodulation protocols for treating visceral pain. In this report, we present an optimized protocol with prolonged urethane infusion that enables robust and repeatable recordings of VMR to CRD in mice under deep anesthesia, providing a two-hour window to objectively assess the efficacy of visceral pain management strategies. During all surgical procedures, C57BL/6 mice of both sexes (8-12 weeks, 25-35 g) were anesthetized with 2% isoflurane inhalation. An abdominal incision was made to allow Teflon-coated stainless steel wire electrodes to be sutured to the oblique abdominal musculature. A thin polyethylene catheter (Φ 0.2 mm) was placed intraperitoneally and externalized from the abdominal incision for delivering the prolonged urethane infusion. A cylindric plastic-film balloon (Φ 8 mm x 15 mm when distended) was inserted intra-anally, and its depth into the colorectum was precisely controlled by measuring the distance between the end of the balloon and the anus. Subsequently, the mouse was switched from isoflurane anesthesia to the new urethane anesthesia protocol, which consisted of a bout of infusion (0.6 g urethane per kg weight, g/kg) administered intraperitoneally via the catheter and continuous low-dose infusion throughout the experiment at 0.15-0.23 g per kg weight per hour (g/kg/h). Using this new anesthesia protocol, we systematically investigated the significant impact of balloon depth into the colorectum on evoked VMR, which showed a progressive reduction with increased balloon insertion depth from the rectal region into the distal colonic region. Intracolonic TNBS treatment induced enhanced VMR to CRD of the colonic region (>10 mm from the anus) only in male mice, whereas colonic VMR was not significantly altered by TNBS in female mice. Conducting VMR to CRD in anesthetized mice using the current protocol will enable future objective assessments of various invasive neuromodulatory strategies for alleviating visceral pain.

Needle-Based Electrical Impedance Imaging Technology for Needle Navigation.

Needle insertion is a common procedure in modern healthcare practices, such as blood sampling, tissue biopsy, and cancer treatment. Various guidance systems have been developed to reduce the risk of incorrect needle positioning. While ultrasound imaging is considered the gold standard, it has limitations such as a lack of spatial resolution and subjective interpretation of 2D images. As an alternative to conventional imaging techniques, we have developed a needle-based electrical impedance imaging system. The system involves the classification of different tissue types using impedance measurements taken with a modified needle and the visualization in a MATLAB Graphical User Interface (GUI) based on the spatial sensitivity distribution of the needle. The needle was equipped with 12 stainless steel wire electrodes, and the sensitive volumes were determined using Finite Element Method (FEM) simulation. A k-Nearest Neighbors (k-NN) algorithm was used to classify different types of tissue phantoms with an average success rate of 70.56% for individual tissue phantoms. The results showed that the classification of the fat tissue phantom was the most successful (60 out of 60 attempts correct), while the success rate decreased for layered tissue structures. The measurement can be controlled in the GUI, and the identified tissues around the needle are displayed in 3D. The average latency between measurement and visualization was 112.1 ms. This work demonstrates the feasibility of using needle-based electrical impedance imaging as an alternative to conventional imaging techniques. Further improvements to the hardware and the algorithm as well as usability testing are required to evaluate the effectiveness of the needle navigation system.

Gastroprotective effect of electrical spinal cord stimulation may be mediated by glucocorticoids produced in response to this stimulation

We demonstrated previously that stress-induced glucocorticoids are gastroprotective hormones but not ulcerogenic ones (Filaretova et al., 2016). Recently electrical spinal cord stimulation began to be used for both experimental studies of motor functions regulation and rehabilitation of motor functions in patients with spinal cord injury (Gerasimenko et al., 2021; Moshonkina et al., 2016). The spinal cord stimulation directed to the activation of spinal locomotor related networks affected visceral systems as well. Recently in our teamwork we have shown that the electrical spinal cord stimulation can cause an increase in blood glucocorticoid level accompanied by a gastroprotective effect (Filaretova et al., 2022). The aim of the present work was to verify the hypothesis that the gastroprotective effect of the electrical spinal cord stimulation may be mediated by glucocorticoids produced in response to this stimulation. Gastric injury was induced in anesthetized rats by prolonged gastric ischemia/reperfusion (I/R, 30 min occlusion of celiac artery followed by 3 h of reperfusion) or indomethacin administration (IM, 35 mg/kg, sc). For spinal stimulation, stainless steel wire electrodes were fixed in fasciae between T11-12 and L1–L2 vertebrae of anesthetized animals. Motor evoked responses in hindlimbs muscles to spinal stimulation were recorded. Spinal stimulation at 30 Hz was carried out with a subthreshold current for inducing muscle contractions (80% of the threshold). Sham-stimulated (control) rats were subjected to the same manipulations except for the stimulation itself. To test the participation of glucocorticoids in the gastroprotective effect of the stimulation, two approaches were used: pretreatment by the inhibitor of glucocorticoid synthesis, metyrapone (30 mg/kg, i.p.) and the antagonist of glucocorticoid receptors RU-38486 (20 mg/kg, i.p.). The concentration of corticosterone in blood plasma was determined using commercial ELISA kits. In control rats the spinal cord stimulation resulted in an increase of blood corticosterone level 1 h later. The spinal stimulation, applied 1 h before the onset of ulcerogenic stimuli, also significantly attenuated gastric erosion formation induced by I/R and IM as well. Metyrapone injected shortly before the stimulation caused a fast inhibition of corticosterone response and reversed the gastroprotective effect stimulation. The gastroprotective effect of the stimulation was also prevented by the pretreatment rats with glucocorticoid receptor antagonist RU-38486. The obtained results indicate on contribution of glucocorticoids to gastroprotective effect of spinal cord stimulation. In conclusion, the findings support the hypothesis that the gastroprotective effect of the electrical spinal cord stimulation may be mediated by glucocorticoids produced in response to this stimulation. The study was supported by the Ministry of Education and Science of the Russian Federation (agreement No. 075-15-2020-921 for the creation and development of the world-class scientific center “Pavlov Center “Integrative Physiology - to medicine, high-tech healthcare and technologies of stress resistance”). This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Effects of Electrical Spinal Cord Stimulation on the Activity of the Hypothalamic‐Pituitary‐Adrenocortical System and the Sensitivity of the Gastric Mucosa to Ulcerogenic Stimuli

Our previous results of two areas of unrelated researches are the basis of the present study. We demonstrated previously that the hypothalamic‐pituitary‐adrenocortical (HPA) axis is gastroprotective component of the brain‐gut axis and stress‐induced glucocorticoids are gastroprotective hormones but not ulcerogenic ones (Filaretova et al., 2016). At the same time recently electrical spinal cord stimulation began to be used for both experimental studies of motor functions regulation and rehabilitation of motor functions in patients with spinal cord injury (Gerasimenko et al., 2021; Moshonkina et al., 2016). The spinal cord stimulation directed to the activation of spinal locomotor related networks affected visceral systems as well. The aim of the present experimental work was to verify whether electrical spinal cord stimulation may activate the HPA axis and affect a sensitivity of the gastric mucosa to ulcerogenic stimuli in rats. Two ulcerogenic stimuli were used in anesthetized rats: 1) prolonged gastric ischemia/reperfusion (I/R, 30 min occlusion of celiac artery followed by 3 h of reperfusion); 2) indomethacin administration (IM, 35 mg/kg, sc). For spinal stimulation, stainless steel wire electrodes (AS632; Cooner Wire, California, USA) were fixed in fasciae between T11‐12 and L1–L2 vertebrae of anesthetized animals. Electrodes for recording motor responses to stimulation placed to the muscles of the hind limbs. A‐M System, Model 2100 stimulator was used for spinal cord stimulation. At the beginning motor thresholds determined by stimulation with monopolar rectangular pulses (1 ms). Then the spinal cord was stimulated with a frequency 30 Hz and a subthreshold current (350–700 μA) in order to exclude the influence of movements for 20 min. Sham‐stimulated (control) rats were subjected to the same manipulations except for the stimulation itself. The concentration of corticosterone in blood plasma was determined using commercial ELISA kits. The spinal cord stimulation resulted in an increase of blood glucocorticoid level 1 h later suggesting an activation of the HPA axis. The spinal stimulation, applied 1 h before the onset of ulcerogenic stimuli, also significantly attenuated gastric erosion formation induced by I/R and IM as well. The data suggest that the electrical spinal cord stimulation may activate the HPA axis and affect a sensitivity of the gastric mucosa to ulcerogenic stimuli in rats producing gastroprotective effect. We consider the results as a basis for further research to verify a possibility of an involvement of glucocorticoids in the mechanisms that may provide the beneficial effects of spinal neuromodulation.

Evaluating Suitable Weld Condition to Obtain Enlarged Bead Width of 316 Stainless Steel Towards Weld Cladding

Usually bead-on-plate welding is performed to standardize process parameters before cladding. Selection of appropriate process parameters plays a vital role for achieving good weld bead geometry. In this experimental investigation, Metal Active Gas Welding is used with 316 austenitic stainless steel wire electrode. An experimental design for Response Surface Methodology named, Box-Behnken (Three Variable) design is adopted for determining the experimental runs. Heat input of 0.6kJ/mm and higher than this can be used to obtain desirable bead-on-plate welding that may also be recommended for weld cladding for this substrate-filler material combine.

In vitro electrochemical assessment of electrodes for neurostimulation in roach biobots.

Biobotics investigates the use of live insects as biological robots whose locomotion can be controlled by neurostimulation through implanted electrodes. Inactivity in the biobots (biological robots) can sometimes be noticed following extended neurostimulation, partly owing to incompatibility of implanted electrodes with the biobotic application or gradual degradation of the tissue-electrode interface. Implanted electrodes need to sufficiently exhibit consistent, reliable, and stable performance during stimulation experiments, have low tissue-electrode impedance, facilitate good charge injection capacity, and be compact in size or shape. Towards the goal of finding such electrodes suitable for biobotic applications, we compare electrochemical performances of five different types of electrodes in vitro with a saline based electrolytic medium. These include stainless steel wire electrodes, microfabricated flexible gold electrodes coated with PEDOT:PSS conductive polymer, eutectic gallium indium (EGaIn) in a tube, and “hybrid” stainless steel electrodes coated with EGaIn. We also performed accelerated aging of the electrodes to help estimate their longitudinal performance. Based on our experimentation, microfabricated electrodes with PEDOT:PSS and stainless steel electrodes coated with EGaIn performed remarkably well. This is the first time conductive polymer and liquid metal electrodes were studied comparatively for neurostimulation applications. These in vitro comparison results will be used in the future to provide a benchmark for subsequent in vivo tests with implanted electrodes in cockroach biobots.

3D printed wire electrode carrier for a pilot study of the functional brain mapping.

In this paper, brain-machine interface (BMI) was adopted to investigate the motor area in the animal brain. Stainless steel wire electrodes were implanted to search the brain area for rotation, forward movement, and flapping. In continuation of our search for optimized coordinates for stimulation, we designed electrode case for safer transport and for a more accurate fabrication of the electrode array. The cases are customized for each set of coordinates, and quickly built using a 3D printer. The case reduced the surgery time and increased accuracy in electrode placement. Stimulation of various sites in the brain derived the pigeon to move as we have anticipated.

Electrohydrodynamic flow patterns and collection efficiency in narrow wire-cylinder type electrostatic precipitator

Recently, narrow electrostatic precipitators (ESPs) have become a subject of interest because of their possible application for the cleaning of the exhaust gases emitted by diesel engines. Diesel engines emit fine particles, which are harmful to human and animal health. There are several methods for decrease particulate emission from a diesel engines, but up to now, these methods are not enough effective or very expensive. Therefore, an electrostatic precipitation was proposed as an alternative method for control of a diesel particulate emission. In this work, results of electrohydrodynamic (EHD) secondary flow and particle collection efficiency measurements in a narrow wire-cylinder type ESP are presented. The ESP was a glass cylinder (300 mm × 29 mm) equipped with a wire discharge electrode and two collecting cylinder-electrodes. A 0.23 mm in diameter and 100 mm long stainless-steel discharge wire electrode was mounted in the center of the cylinder, parallel to the main flow direction. The collecting electrodes were made of stainless steel cylinders, each with a length of 100 mm and inner diameter of 25.5 mm. An air flow seeded with a cigarette smoke was blown along the ESP duct with an average velocity of 0.9 m/s. The EHD secondary flow was measured using 2-dimensional particle image velocimetry (PIV) method. The PIV measurements were carried out in the wire electrode mid-plane, perpendicularly to the wire and the collecting electrodes. The results show similarities and differences of the particle flow in the wire-cylinder type ESP for a negative and a positive DC voltage polarity. The collection efficiency was calculated from the fractional particle concentration. The fractional particle concentration was measured using the optical aerosol spectrometer. The results of the fractional collection efficiency confirmed the common view that the collection efficiency of fine particles in the ESP increases with increasing voltage and it is higher for negative voltage polarity and decreases when decreasing particle diameter.

Carbon nanotube coating improves neuronal recordings

Implanting electrical devices in the nervous system to treat neural diseases is becoming very common. The success of these brain-machine interfaces depends on the electrodes that come into contact with the neural tissue. Here we show that conventional tungsten and stainless steel wire electrodes can be coated with carbon nanotubes using electrochemical techniques under ambient conditions. The carbon nanotube coating enhanced both recording and electrical stimulation of neurons in culture, rats and monkeys by decreasing the electrode impedance and increasing charge transfer. Carbon nanotube-coated electrodes are expected to improve current electrophysiological techniques and to facilitate the development of long-lasting brain-machine interface devices.

The effect of dielectric barrier discharge configuration on the surface modification of aromatic polymers

Here, we report on the oxygenation and roughness features of polystyrene and polyethylene terephthalate films, induced by air dielectric barrier discharge (DBD) processing using different electrode–platen configurations. The combinations of triple electrode–platen used were stainless steel wire electrodes–rubber platen (WE–RP), ceramic electrodes–aluminium platen (CE–AP) and quartz electrodes–aluminium platen (QE–AP). The degree of oxygenation of polymer surfaces achieved by processing in the QE–AP combination was comparatively higher (as observed by x-ray photoelectron spectroscopy) than that for the other discharge configurations used. The surface roughness following DBD processing with the CE–AP set was found to be critically higher (by atomic force microscopy) than with the WE–RP and QE–AP combinations. We conclude that the localized physical damage of the polymer surface caused by the microfilaments present in the discharge can be avoided by suitably optimizing the experimental conditions, i.e. the appropriate use of electrode–platen system, the speed of processing (or residence time) and the operating power.

Surface oxygenation of polypropylene using an air dielectric barrier discharge: the effect of different electrode?platen combinations

Polypropylene film has been modified in an air dielectric barrier discharge using two different electrode–platen configurations: stainless steel wire electrode–rubber platen or ceramic electrode–aluminium platen combinations. Modified films were characterised by static contact angle measurements, X-ray photoelectron spectroscopy (XPS), secondary ion mass spectrometry (SIMS) and Fourier transform infrared spectroscopy (ATR–FT-IR). Surface hydrophilic modification appears to be governed by the presence of low-molecular weight oxidised functionalities using XPS and SIMS techniques. Irrespective of the type of electrode–platen combination used to obtain the discharge, oxygenated functionalities of identical nature are formed on the polymer surface. However, the degree of oxidation obtained by the discharge using the wire electrodes with the rubber platen was considerably greater. Further increase in the observed hydrophilicity due to molecular rearrangement and development of stable oxygenated functionalities was evident after 1 month of post-processing analysis.

Surface oxygenation of polypropylene using an air dielectric barrier discharge: the effect of different electrode–platen combinations

Polypropylene film has been modified in an air dielectric barrier discharge using two different electrode–platen configurations: stainless steel wire electrode–rubber platen or ceramic electrode–aluminium platen combinations. Modified films were characterised by static contact angle measurements, X-ray photoelectron spectroscopy (XPS), secondary ion mass spectrometry (SIMS) and Fourier transform infrared spectroscopy (ATR–FT-IR). Surface hydrophilic modification appears to be governed by the presence of low-molecular weight oxidised functionalities using XPS and SIMS techniques. Irrespective of the type of electrode–platen combination used to obtain the discharge, oxygenated functionalities of identical nature are formed on the polymer surface. However, the degree of oxidation obtained by the discharge using the wire electrodes with the rubber platen was considerably greater. Further increase in the observed hydrophilicity due to molecular rearrangement and development of stable oxygenated functionalities was evident after 1 month of post-processing analysis.

A motion of forearm supination with maintenance of elbow flexion produced by electrical stimulation to two elbow flexors in humans

Motions of the forearm induced by electrical stimulation to two elbow flexors (brachioradialis: BR, biceps brachii: BB) were examined in five healthy human subjects. Stainless steel wire electrodes were implanted percutaneously into each motor point of the muscles. The muscles were stimulated separately with a computer-controlled multi-channel stimulator. The motions were taken with a digital video system. Angular changes of the motions in elbow flexion/extension and forearm pronation/supination were measured. Electromyograms (EMG) of BR, BB, and the triceps brachii (TB) were recorded. Electrical stimulation to BR induced a motion of flexion and that to BB motions of flexion and supination. The stimulation to BR with an adequate intensity provided holding of flexion with the prone forearm in all the subjects. In this situation, additional stimulation to BB resulted in motions of flexion and supination. However, the additional stimulation accompanied with a decrease of the stimulation intensity for BR provided a motion of supination with maintenance of the flexion in all the subjects. Since during the stimulation BR, BB, and TB showed no voluntary contraction in EMG, it is suggested that modulation of contraction between BR and BB by the stimulation can produce force in supination with keeping constant force in flexion to support the weight below the elbow.

Evaluation of different recording parameters to establish a standard for flash electroretinography in rodents

Different electrodes and stimulus protocols commonly used for electroretinography in rodent eyes were compared for convenience of use, degree of damage to corneal epithelium, and for magnitude of amplitude, reproducibility, left versus right eye accuracy, and reliability of recorded parameters of the flash electroretinogram (ERG). Adult C57BL/6 pigmented mice and albino Wistar rats were used to determine scotopic ERGs in response to Ganzfeld or strobe-light stimulation and light-adapted (photopic) ERGs recorded from both eyes at the same time. Test–retest data were used for statistical analyses to compare a monopolar gold-wire contact lens electrode (CLE), a cotton-wick silver–silver chloride electrode (CSCE), a DTL fiber electrode (DTLE), and a circular stainless steel wire electrode (SSE). Corneas were evaluated for abrasion after ERG recordings using fluorescein staining and also for the time taken, ease of insertion, and re-insertions required for the different electrodes. Compared to CSCE, DTLE, and SSE, the ERG potentials recorded by CLE had significantly larger scotopic amplitudes and oscillatory potentials under strobe or Ganzfeld stimulation and for light-adapted ERG b-wave amplitudes in both mice and rats. In analyzing test–retest data of scotopic ERG a-wave and b-wave amplitudes, the intraclass correlation coefficient showed the best agreement for the CLE (range 0.61–0.94) compared to the SSE (0.13–0.77), DTLE (0.02–0.69), and CSCE (0.12–0.51). In mice and rats, logistic regression analyses revealed significant correlations for amplitudes of most scotopic ERG parameters between contralateral eyes obtained with CLE and for some ERG components recorded by SSE. When comparing ERG amplitudes for stimulation by strobe or Ganzfeld, the difference was least with the CLE compared to DTLE, CSCE, or SSE. The time taken to insert the four different electrodes was greatest for the CLE in both mice and rats. The extent of corneal abrasion resulting from electrode use in mice was largest for the SSE followed by the CLE. However, in rats there was almost no corneal damage after ERG recordings with the CLE. Because of the stability of eye contact, the CLE allows ERGs to be determined over a longer recording session. Recording of scotopic and photopic (light-adapted) ERGs in rodents with monopolar gold-wire contact lens electrodes provides greater amplitudes and higher reproducibility when compared to other commonly used corneal electrodes. These electrodes are significantly better overall than others that were evaluated and should be considered for a standard protocol to monitor retinal function in rodent eyes.

Microprocessor-controlled movement of solid gastric content using sequential neural electrical stimulation

Background & Aims: Gastric electrical stimulation has been attempted for years without much success. The aim of this study was to determine if movement of solid gastric content could be achieved using microprocessor-controlled sequential electrical stimulation. Methods: The study was performed on 9 anesthetized dogs. The dogs underwent laparotomy, pyloroplasty, and implantation of 4–6 sets of bipolar stainless-steel wire electrodes. Each set consisted of 2–6 electrodes (10 × 0.25 mm, 3 cm apart) implanted circumferentially. The stomach was filled with solid food mixed with plastic pellets, and the process of gastric emptying was monitored. Artificial contractions were produced using microprocessor-controlled phase-locked bipolar trains of 50-Hz rectangular voltage with flexible amplitudes. Results: Using the above stimulating parameters, we were able to produce circumferential gastric contractions that were artificially propagated distally by embedding and phase-locking the stimulating voltage. The number of expelled pellets after the stimulation sessions was significantly higher than the number of pellets emptied during the nonstimulation sessions ( P < 0.01). Conclusions: Microprocessor-controlled electrical stimulation produced artificial peristalsis and markedly accelerated the movement of solid gastric content. GASTROENTEROLOGY 2000;118:258-263

Performance of temporary epicardial stainless steel wire electrodes used to treat atrial fibrillation: a study in patients following open heart surgery.

AF is the most common arrhythmia following open heart surgery. Transthoracic cardioversion is used when pharmacological treatment fails to restore SR, or is ineffective in controlling ventricular response rate. We report on the performance of temporary atrial defibrillation wire electrodes implanted on the epicardium of patients undergoing open heart surgery. Epicardial stainless steel wire electrodes for both pacing/sensing and atrial defibrillation were placed at the left and right atrium during open heart surgery in 100 consecutive patients (age 65 +/- 9 years; male/female 77/23). Electrophysiological studies performed postoperatively revealed a test shock (0.3 J) impedance of 96 +/- 12 omega (monophasic) and 97 +/- 13 omega (biphasic). AF was induced by burst stimulation in 84 patients. All patients were successfully converted to SR. The mean energy of successful shocks was 3.1 +/- 1.9 J. Atrial pacing and sensing were accomplished in all patients. Pacing threshold was 1.9 +/- 1.7 V (0.5 ms) in the left atrium and 2.1 +/- 2 V in the right atrium. P wave sensing was 2.5 +/- 1.6 mV in the left atrium and 2.3 +/- 1.4 mV in the right atrium. No complications were observed with shock application, nor with lead extraction. Atrial defibrillation using temporary epicardial wire electrodes can be performed successfully and safely in patients following cardiac operations. The shock energy required to restore SR is low. Electrical cardioversion in the absence of anesthesia should be feasible.

Internal atrial defibrillation - a new treatment of postoperative atrial fibrillation.

Atrial fibrillation (AF) commonly occurs following open heart surgery and may delay hospital discharge. Transthoracic electrical cardioversion is used when medical treatment is ineffective or associated with side effects. Traditionally general anesthesia is required. The aim of this multicenter study was to examine the feasibility and efficacy of low-energy atrial defibrillation using temporary epicardial defibrillation wire electrodes. Epicardial stainless-steel defibrillation wire electrodes were sutured onto the left and right atrium during open heart surgery in 238 patients (age 64+/-9 years; 180 males). In case of postoperative AF R-wave synchronous low-energy shocks (0.6-10.8J) were applied to achieve cardioversion without anesthesia. Implantation of the electrodes added 4.3+/-2.8 minutes to the operating time. During the hospital stay AF occurred in 47 patients (20%) at 2.1+/-1.3 days postoperatively. Fifty-one episodes of AF occurring in 44 patients were treated by atrial defibrillation. Primary success rate of cardioversion was 33/51 (65%). Early recurrence of AF (within 60 seconds after defibrillation) developed in 15 patients. Seven of these 15 patients were successfully defibrillated later on. Overall success rate was 40/51 (78%). The mean energy of successful shocks was 5.8+/-2.7 J. A mean of 2.3+/-1.7 shocks were applied per patient. The shocks were well tolerated by the patients in the absence of anesthesia. No complications were observed with shock application or with lead extraction. Atrial defibrillation using temporary epicardial wire electrodes can be performed safely and effectively in patients following cardiac surgery. The shock energy required to restore sinus rhythm is low.

Microprocessor controlled movement of liquid gastric content using sequential neural electrical stimulation

Background—Gastric electrical stimulation has been attempted for several years with little success.Aims—To determine whether movement of liquid gastric content could be achieved using microprocessor controlled sequential electrical stimulation.Methods—Eight anaesthetised dogs...

Reliability of percent distribution of power of the electrogastrogram in recognizing gastric electrical uncoupling

The aim of this study was to examine the reliability of percent distribution of electrogastrographic (EGG) power in recognizing gastric electrical uncoupling. Sixteen anaesthetized dogs underwent laparotomy and implantation of six pairs of stainless-steel wire electrodes. Distal stomach was measured and three sections with approximately equal lengths were defined. Two pairs of electrodes were implanted in each section. Eight-channel EGG was also recorded. Three separate half-hour recordings were made: in the basal state; after a full circumferential separation of the distal antral section from the rest; after a second circumferential cut completely separating the middle from the proximal sections. EGG digital power spectra were split into three frequency ranges and dynamics of percent distribution of power was statistically examined. After the first cut, changes in the percent distribution of EGG power in the normal range were not significant (p = 0.2). Significant changes in the low range were noted (p < 0.05) and changes in the high range were borderline nonsignificant (p = 0.056). After the second cut, changes in percent distribution in the normal and the high range became significant (p < 0.01) while changes in the low range were insignificant (p = 0.075). Severe uncoupling was reflected in EGG by significant changes in the high-frequency range without internal tachygastria necessarily being present.

Successful atrial defibrillation with very-low-energy shocks by means of temporary epicardial wire electrodes

Sustained atrial fibrillation is very common after cardiac surgical procedures. We hypothesized that atrial defibrillation could be accomplished consistently and safely by means of low-energy shocks delivered by temporary stainless steel wire electrodes placed at the time of the operation. Sterile pericarditis was created in five mongrel dogs (20.9 ± 2.1 kg), and pairs of standard temporary stainless steel wire electrodes were placed on the right atrial appendage, on Bachmann's bundle, and on the right ventricular apex for pacing, sensing, and recording. Temporary stainless steel wire electrodes, insulated except for the distal 6 cm and used to deliver defibrillation shocks, were placed adjacent to both atrial free walls and secured to the pericardium. All electrodes were brought out through the skin, and the sternotomy was closed. Dogs were tested in the conscious state on postoperative day 2. Sustained atrial fibrillation was induced by rapid atrial pacing. A customized software program was used to control the defibrillator, which delivered R wave–synchronous biphasic shocks to the atria through the temporary defibrillation electrodes. The shock intensity began at 50 volts and was increased by 10-volt increments until atrial fibrillation was terminated. Atrial fibrillation was terminated in all dogs at 112 ± 7 volts, with an energy of 0.42 ± 0.07 joule and an impedance of 67.8 ± 4 ohms (all values mean ± standard error of the mean). The mean percent success for atrial defibrillation at this minimal threshold was 49%. Thus at low-threshold voltages atrial fibrillation could be terminated with every other shock. A 25% increase in the minimal threshold voltage improved the conversion rate to 73% (mean energy 0.66 ± 0.19 joule and mean impedance of 67 ± 3.8 ohms). No complications were detected with the use of the electrodes or after their removal on the seventh postoperative day. One instance of electrode migration on the right atrial free wall was detected by roentgenography, but this did not adversely affect atrial defibrillation threshold. No ventricular arrhythmias or hemodynamic complications were noted during shock delivery. We conclude that successful conversion of atrial fibrillation to sinus rhythm can be achieved consistently with shock energies below 0.5 joule delivered with temporary epicardial defibrillation wire electrodes in this canine pericarditis model. These results suggest that this approach to the management of sustained atrial tachyarrhythmias has considerable promise in the management of atrial fibrillation in patients who have had cardiac operations. (J T HORAC C ARDIOVASC S URG 1996;111:392-8)