Gold nanorod stain deposits have been studied using helium ion microscopy (HIM). The multilayer thick deposits can become well-organized monolayers when the density drops, as demonstrated by the density fluctuation of nanosuspension. However, once the density of nanoparticles approaches certain extremely low values, this tendency has disappeared. The difference in density associated with the liquid crystalline (LC) phases that have developed on the surface. There is a correlation between the density of nanoparticles in suspension and the relationship between droplet volume and UV-Vis absorbance. The physical characteristics that affect and generate such LC phases are contrasted and examined with the assembly method. To highlight noteworthy aspects, a quantitative comparison between the HIM analysis and the SEM is also made. Analyzing the correlation between the coffee stain range, nanoparticle density, and droplet contact angle is essential. It is possible to quantify the difference in the ring stains from the outside to the midle and from the midle to the inner boundary. HIM is typically useful when attempting to determine the true composition of surface deposits. The aim of this work is to investigate the effects of particle density on liquid crystalline superstructures and the coffee stain effect.