Swelling Stage Research Articles

Neoadjuvant Immunotherapy and De-Escalation of Surgery in Locally Advanced Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL)

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare form of non-Hodgkin T cell lymphoma diagnosed in patients with a history of breast implants. Most patients develop a periprosthetic effusion at early stages of disease while less common presentations include a palpable mass, severe capsular contracture, lymphadenopathy, or cutaneous erythema. Due to the complex nature of this disease, a multidisciplinary approach is necessary for optimal management, particularly in locally advanced disease or inoperable patients. We present the successful use of neoadjuvant therapeutic protocols in two cases of locally advanced BIA-ALCL. The first case, a 52-year-old patient with a left breast mass-like stage III disease who underwent combined targeted immunotherapy and chemotherapy (BV-CHP). Following a complete radiological and metabolic response, the patient underwent bilateral implant removal, right total intact capsulectomy, left en bloc capsulectomy and skin resection from the left inframammary fold in continuity with the capsule. The second case, a 65-year-old patient with a right breast swelling and mass-like stage IIA disease who received targeted immunotherapy, Brentuximab vedotin (BV). Following a complete metabolic response, she underwent bilateral implant removal and en bloc capsulectomy. A literature review and the reported cases suggest the effectiveness of targeted immunotherapy as monotherapy or in combination with chemotherapy in locally advanced BIA-ALCL in disease downstaging, surgical de-escalation, reduction of significant post-operative complications and an acceptable tolerance profile. Although surgery is an essential part of treatment, timing and type of intervention should be carefully planned, especially when primary, radical resection is uncertain.

Processes of assembly of endophytic prokaryotic and rhizosphere fungal communities in table grape are preponderantly deterministic

Understanding the assemblage of microbial communities is important for the health maintenance and post-harvest quality of fruit crops. However, systematic studies on the core microbiomes of table grapes and microbial community assembly of soil-plant continuum are still rare. To address this, samples were collected and analyzed from key microbiome compartments (bulk soil, rhizosphere of new roots and old roots, endosphere of new and old roots, and endosphere of leaves and fruits) of five table grape cultivars (Balado Black, Jingya, Fujimino, Victoria and Kyoho) at the swelling stage and harvest stage in the field plots. Prokaryotic and fungal communities were analyzed using 16S rRNA and ITS rRNA high-throughput sequencing, respectively. The compartment was the major factor attributable to the variations in both prokaryotic (52.5 %) and fungal communities (23.2 %), respectively. The richness and phylogenetic diversity of both prokaryotic and fungal communities decreased significantly along the compartment continuum from soil to fruit endosphere, but did not differ between the five cultivars across all samples. Occupancy-abundance distribution was used to define 97 core bacteria, 29 core fungi for rhizosphere soil and five core bacteria, 172 core fungi for endosphere. Functional annotations of the rhizosphere core bacteria were mostly associated with ureolysis and chemoheterotrophy, while endophytic core bacteria were mostly fermentative and symbiotic. Rhizosphere core fungi were saprophytic and the endophytic fungi were putatively pathogens. From rhizosphere soil to aboveground organs, the relative importance of deterministic processes for prokaryotic community assembly gradually increased but the contribution of stochastic processes increased for fungal community assembly. These findings highlight niche-specific microbial communities and shared core microbiomes in different grapevine cultivars. The deterministic processes contribute more to the assembly of prokaryotic communities in the aboveground than in the belowground while the assembly of fungal communities exhibits opposite trends. Management practices aiming at niche-specific microbiome should be developed for sustainable grape production.

Telomere-to-telomere genome assembly of Eleocharis dulcis and expression profiles during corm development

Eleocharis dulcis (Burm. f.) Trin. ex Hensch., commonly known as Chinese water chestnut, is a traditional aquatic vegetable in China, and now is widely cultivated throughout the world because of its high nutritional value and unique tastes. Here, we report the assembly of a 493.24 Mb telomere-to-telomere (T2T) genome of E. dulcis accomplished by integrating ONT ultra-long reads, PacBio long reads and Hi-C data. The reference genome was anchored onto 111 gap-free chromosomes, containing 48.31% repeat elements and 33,493 predicted protein-coding genes. Whole genome duplication (WGD) and inter-genomic synteny analyses indicated that chromosome breakage and genome duplication in E. dulcis possibly occurred multiple times during genome evolution after its divergence from a common ancestor with Rhynchospora breviuscula at ca. 35.6 Mya. A comparative time-course transcriptome analysis of corm development revealed different patterns of gene expression between cultivated and wild accessions with the highest number of differentially expressed genes (DEGs, 15,870) at the middle swelling stage and some of the DEGs were significantly enriched for starch metabolic process.

Transcriptome and metabolite profiles reveal the role of benzothiadiazole in controlling isoprenoid synthesis and berry ripening in chardonnay grapes

Benzothiadiazole (BTH) regulates grape development, ripening, volatiles, and phenolics. This study used metabolomics and transcriptomics to understand how exogenous BTH affects Chardonnay grapes' maturation and synthesis of isoprenoids. A 0.37 mM BTH solution was sprayed during the swelling and veraison stages, and then the ripe grapes were analyzed. Our results show that BTH application significantly increased levels of important isoprenoids such as free terpinen-4-ol, bound linalool, and 8′-apo-β-carotenal. Additionally, BTH was found to modulate several signaling pathways, including those involved in ethylene biosynthesis, salicylic acid synthesis, the abscisic acid pathway, and sugar metabolism, by regulating the expression of genes like VvACO4, VvTAR, VvPLD, VvTIP1–1, VvSTKs, VvPK, VvSUC2, VvGST4, and VvSTS. BTH also promoted grapevine resistance by up-regulating the expression of VvHSP20, VvGOLS4, VvOLP, and VvPR-10. Furthermore, BTH affected isoprenoids biosynthesis by regulating the expression of VvTPS35 and VvMYB24. Moreover, 13 hub genes in the MEgreen module were identified as crucial for the biosynthesis of isoprenoids. BTH application during the swelling stage remarkably promoted isoprenoid biosynthesis more effectively than veraison. Our study provides insights into the molecular mechanisms underlying BTH-induced regulation of grape development and offers a promising approach for enhancing the quality and resistance of grapes.

Research on the Interaction Mechanisms between ScCO2 and Low-Rank/High-Rank Coal with the ReaxFF-MD Force Field.

CO2 geological sequestration in coal seams can be carried out to achieve the dual objectives of CO2 emission reduction and enhanced coalbed methane production, making it a highly promising carbon capture and storage technology. However, the injection of CO2 into coal reservoirs in the form of supercritical fluid (ScCO2) leads to complex physicochemical reactions with the coal seam, altering the properties of the coal reservoir and impacting the effectiveness of CO2 sequestration and methane production enhancement. In this paper, theoretical calculations based on ReaxFF-MD were conducted to study the interaction mechanism between ScCO2 and the macromolecular structures of both low-rank and high-rank coal, to address the limitations of experimental methods. The reaction of ScCO2 with low-rank coal and high-rank coal exhibited significant differences. At the swelling stage, the low-rank coal experienced a decrease in aromatic structure and aliphatic structure, and high-rank coal showed an increase in aromatic structure and a decrease in aliphatic structure, while the swelling phenomenon was more pronounced in high-rank coal. At the dissolution stage, low-rank coal was initially decomposed into two secondary molecular fragments, and then these recombined to form a new molecular structure; the aromatic structure increased and the aliphatic structure decreased. In contrast, high-rank coal showed the occurrence of stretches-breakage-movement-reconnection, a reduction in aromatic structure, and an increase in aliphatic structure. The primary reasons for these variations lie in the distinct molecular structure compositions and the properties of ScCO2, leading to different reaction pathways of the functional group and aromatic structure. The reaction pathways of functional groups and aromatic structures in coal can be summarized as follows: the breakage of the O-H bond in hydroxyl groups, the breakage of the C-OH bond in carboxyl groups, the transformation of aliphatic structures into smaller hydrocarbon compounds or the formation of long-chain alkenes, and various pathways involving the breakage, rearrangement, and recombination of aromatic structures. In low-rank coal, there is a higher abundance of oxygen-containing functional groups and aliphatic structures. The breakage of O-H and C-OH chemical bonds results in the formation of free radical ions, while some aliphatic structures detach to produce hydrocarbons. Additionally, some of these aliphatic structures combine with carbonyl groups and free radical ions to generate new aromatic structures. Conversely, in high-rank coal, a lower content of oxygen-containing functional groups and aliphatic structures, along with stronger intramolecular forces, results in fewer chemical bond breakages and makes it less conducive to the formation of new aromatic structures. These results elucidate the specific deformations of different chemical groups, offering a molecular-level understanding of the interaction between CO2 and coal.

Distribution, Residue Dynamics, and Insecticidal Efficacy of Trunk-Injected Emamectin Benzoate in Pecan Trees

Carya illinoinensis (Wangenh.) K. Koch (Pecan), a deciduous tree native to North America, faces significant challenges from pests. This study investigated the insecticidal efficacy and food safety of using the broad-spectrum insecticide emamectin benzoate via trunk injection for potential pecan pest management. Injections were given at dosages of 0.4, 0.8, 1.6, and 2.4 mL/cm of tree diameter at breast height (DBH), and leaf samples were collected at 10, 30, 60, and 100 days post-injection, while fruit samples were obtained during the swelling, ripening, and harvest stages. We established an analytical method for the determination and quantification of emamectin benzoate content in pecans using ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). Leaf emamectin benzoate content was significantly higher compared to nuts (p ≤ 0.036). The content in leaves following the four dosage treatments decreased over time and at 100 days was 0.1943/0.2799 mg/kg (upper crown/lower crown), 0.1910/0.3957 mg/kg, 0.3663/0.6235 mg/kg, and 1.3988/1.9123 mg/kg, respectively. The pesticide residues of 0.4 mL/cm and 0.8 mL/cm treatment groups in kernels at harvest time were 0.0016 mg/kg and 0.0039 mg/kg, respectively, below the latest European Union Regulation (0.005 mg/kg). All four dosage treatments (0.4, 0.8, 1.6, and 2.4 mL/cm of tree diameter at DBH) in the leaf feeding test caused significant mortalities of the fourth instar Hyphantria cunea (Drury) larvae. The mortality rates at 10 days post-injection were 64.7%, 73.3%, 79.3%, and 84.7%, respectively, while at 60 days post-injection, the rates were 26.0%, 47.3%, 53.7%, and 81.7%, respectively. In summary, this study successfully established a sensitive analytical method for the detection and quantification of trunk-injected emamectin benzoate residues in pecans and demonstrates its safety and effectiveness as a chemical control option against foliar pecan pest insects.

Morphological responses of filamentous fungi to stressful environmental conditions.

The filamentous growth mode of fungi, with its modular design, facilitates fungal adaptation to stresses they encounter in diverse terrestrial and anthropogenic environments. Surface growth conditions elicit diverse morphological responses in filamentous fungi, particularly demonstrating the remarkable adaptability of mycelial systems to metal- and mineral-rich environments. These responses are coupled with fungal biogeochemical activity and can ameliorate hostile conditions. A tessellated agar tile system, mimicking natural environmental heterogeneity, revealed negative chemotropism to toxic metals, distinct extreme growth strategies, such as phalanx and guerrilla movements and transitions between them, and the formation of aggregated re-allocation structures (strands, cords, synnemata). Other systems showed intrahyphal growth, intense biomineralization, and extracellular hair-like structures. Studies on submerged mycelial growth, using the thermophilic fungus Thielavia terrestris as an example, provided mechanistic insights into the morphogenesis of two extreme forms of fungal submerged culture-pelleted and dispersed growth. It was found that the development of fungal pellets was related to fungal adaptation to unfavorable stressful conditions. The two key elements affecting morphogenesis leading to the formation of either pelleted or dispersed growth were found to be (1) a lag phase (or conidia swelling stage) as a specific period of fungal morphogenesis when a certain growth form is programmed in response to morphogenic stressors, and (2) cAMP as a secondary messenger of cell signaling, defining the implementation of the particular growth strategy. These findings can contribute to knowledge of fungal-based biotechnologies, providing a means for controllable industrial processes at both morphological and physiological levels.

Aspectos fenológicos e produtivos de variedades de uvas submetidas a duas épocas de poda no Vale do Rio do Peixe-SC

Abstract: Pruning of grape varieties for processing typically occurs in winter. However, due to challenges in availability and labor costs, there is interest in shifting this practice to autumn—a period with lower demand for vineyard activities. This study aimed to assess the direct influence of autumn pruning on the phenological and productive performance of Niágara Rosada, Isabel Precoce, and Bordô varieties in the Vale do Rio do Peixe region, SC. Experiments were conducted during the 2015, 2016, 2017, and 2018 harvests in fully productive commercial vineyards located in Videira-SC. Pruning occurred in May (autumn) and August (winter). Autumn pruning took place when approximately 5% of the leaves had fallen, and the rest had turned yellow. Winter pruning occurred during the bud swelling stage. Weekly phenological monitoring, as well as measurement of the plants’ productive characteristics and the physical-chemical composition of the grapes, were conducted. Autumn pruning enhanced the productivity, average bunch weight, and uniformity of maturation for Niagara Rosada but accelerated the bud break for this variety in Vale do Rio do Peixe, SC, Brazil. In Bordô, autumn pruning did not affect productivity, but it advanced bud break. Conducting autumn pruning for four consecutive years decreased the productivity of Isabel Precoce.

Study on water-absorbing behaviors of superabsorbent polymer enhanced by 1,2,3,4-butane tetracarboxylic acid cross-linker

superabsorbent polymers (SAPs) are famous for their excellent water absorption capability. In this work, poly (acrylic acid)-based superabsorbent polymer was developed via free radical polymerization by innovatively using 1,2,3,4-butane tetracarboxylic acid (BTA) as one unique physical cross-linker. Water-soluble BTA molecules contain four carboxyl groups that can interact with poly(acrylic acid) (PAA) polymeric chains through hydrogen bonds. Such force made the products swell but not dissolve in water, determining that BTA took a great role of cross-links. The effects of initiator, catalyzer, neutralizer and cross-linking agent on water-absorbing capacities of as-obtained PAA/BTA-SAP products were explored. The results revealed that swelling ratios of such PAA/BTA-SAP in distilled water was 4480 g/g, in 0.9 wt% NaCl solution was 55 g/g and in 5 wt% urea solution was 1890 g/g. Swelling kinetics indicated that polymer chains of the product in distilled water underwent relaxation and stretching in the swelling stage. The excellent water uptake was attributed to the formation of a well-structured three-dimensional network for SAPs via multiple hydrogen bonds between PAA polymer and BTA molecule. Therefore, the present work provides a new horizon to explore BTA as one unique cross-linker to prepare SAPs with the enhanced water uptake.

Properties of Bentonite-Based Sealing Materials during Hydration

A typical deep geological repository (DGR) design consists of a multi-barrier system, including the natural host rock and the engineered barrier system. Understanding the swelling behavior of bentonite-based sealing materials (BBSM), as a candidate material for the engineered barrier system, is crucial for DGR’s long-term safety. In this study, a hydromechanical (HM) column-type test was designed to model the hydration of BBSM from the underground water and determine the resulting swelling pressure in vertical and radial directions. Five hydration tests were carried out on identical compacted samples of 70% bentonite and 30% sand (70-30 bentonite-sand) mixtures with a dry density of 1.65 g/cm3 for varied durations of hydration, between 1 day and 120 days. The experiments were performed parallel to the compaction direction. Following each HM column-type test, the advancement of the wetting front was determined for each test. After 120 days, 56,339 mm3 of water infiltrated the sample and the wetting front reached over 50% of the sample height. The evolution of axial swelling pressure revealed an initial increase in swelling pressure with time in all tests, followed by a reduction in the rate at later times. After early stages of swelling, radial sensors showed an increase in swelling pressure. After 120 days, the radial pressure sensor closest to the hydration front showed 52% more radial pressure than the axial swelling pressure.

Ecological succession of blow flies (Diptera: Calliphoridae) in the decomposition of pig carcasses in Cerrado areas of Brazil.

This research aims to describe the process of ecological succession by associating the decomposition stages of pig carcasses with flies from the Calliphoridae family (Diptera). For this, 6 pig carcasses were exposed in Maranhão's Cerrado, utilizing metal cages with sawdust trays to catch immature specimens and "suspended traps" to capture adults. Adults of the Hemilucilia benoisti Séguy, and Hemilucilia townsendi Shannon species were only associated with the swelling stage. Chrysomya megacephala (Fabricius) was associated with 2 stages (black putrefaction and fermentation). The species Chloroprocta idioidea (Robineau-Desvoidy), Chrysomya albiceps (Wiedemann), Cochliomyia macellaria (Fabricius), and Lucilia eximia (Wiedemann) were associated with 4 of the 5 stages of decomposition, the latter being the only 1 associated with the initial stage. The larvae abandoned the carcasses to pupate from the second stage of decomposition, with L. eximia being the only 1 leaving the carcasses in the swelling stage, and C. albiceps the only 1 associated with both the fermentation and dry stages. Our findings indicate that calliphorid species can help forensic investigators estimine the post-mortem interval of cadavers in situations similar to those detailed in this study. Since there was a link between adult and immature species and certain stages, they can be used as indicators in future forensic investigations.

Влияние хелатных микроудобрений на интенсивность набухания и прорастания семян зернового сорго

The results of three-year studies in 2020-2023 have shown the prospects of processing seeds of grain sorghum varieties with solutions of chelated micronutrients that contribute to the activation of physiological and biochemical processes at the initial stages of swelling and germination of grains. There was a tendency to increase the swelling of seeds during 2 days of the experiment on average in varieties under the action of chelated micronutrients: with the use of Reasil Forte Carb-Ca/Mg/B Amino up to 67.7%, and Reasil micro Amino Zn – up to 67.5%, which is higher than the control variant (66.3%). The most responsive varieties for the use of the studied micronutrients were the Bachelor varieties, the seed germination energy of which increased by 9.7%-15.4% compared to the control variant, the Garant variety – 9.2-15.0%, the RSK Cascade variety – 8.5-11.6%. A significant increase in the length of seedlings and roots of grain sorghum varieties was revealed as a result of soaking seeds in solutions of chelated micronutrients before the experiment. The individual reaction of each variety to the use of chelated micronutrients in seed treatment has also been determined. The varieties Bachelor, Assistant, Guarantor, RSC Cascade, RSC Locus, Pendant and Prince, when treating seeds with Reasil Forte Carb-Ca/Mg/B Amino, significantly increased the length of seedlings compared to the control variant - by 10.6–21.8%. The drug Reasil micro Amino Zn caused the formation of sorghum seedlings of greater length than in the control in the varieties Magister, Assistant, Garant and RSC Cascade, the excess of the control variant was 10.0-28.1%. The greatest positive response to the use of chelated micronutrients was distinguished by the Assistant variety, which, as a result of seed treatment with these preparations, revealed an increase in the length of primary seedlings and roots greater than the control variant by 20.5–28.1%, respectively. Keywords: GRAIN SORGHUM, VARIETY, CHELATED FERTILIZERS, SEED SWELLING, GERMINATION ENERGY, EVALUATION OF PRIMARY SPROUTS

Research on Damage Mechanism and Mechanical Characteristics of Coal Rock under Water Immersion

This study aims to reveal the impact of immersion duration on the internal structural damage and mechanical property degradation of coal rocks. Coal rocks from the post-mining area of Liangshuijing Coal Mine were selected as the research subject. Physical and mechanical tests were carried out on these with different immersion durations (0 d, 15 d, 30 d, 60 d, 120 d, and 240 d) using scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), acoustic emission (AE), digital image correlation (DIC), and compression testing, further elucidating the damage degradation mechanisms of water-immersed coal rocks. The research demonstrates that changes in the pore structure of coal rocks can be divided into two stages as the soaking time varies: the stage of water swelling (saturation process) and the stage of soaking damage (long-term immersion process). The water swelling stage of coal rock extends from surface drying and contraction to water swelling, and the soaking damage stage of coal rock extends from expansion to soaking damage. During the stage of soaking damage, the water showed dynamic changes from macropores to mesopores to micropores, with a gradual increase in the number of micropores. The AE count and cumulative count of coal rock decrease first and then increase, and the four stages’ acoustic characteristics and macroscopic characterization phenomena appear. The mechanical properties declined. After 240 d of immersion, the uniaxial compressive strength and elastic modulus decreased by 48.93% and 29.53%, respectively, and the plastic characteristics were enhanced. These research results provide a beneficial reference for understanding and predicting the instability and destruction of water-immersed coal rocks.

Estimating Fertilizer Nitrogen-Use Efficiency in Transplanted Short-Day Onion

Efficient nitrogen (N) fertilizer applications in onion (Allium cepa L.) can reduce input costs and improve fertilizer-use efficiency, while maintaining high yields and quality. Understanding the N requirements of onion at different growth stages is necessary to enhance fertilizer N-use efficiency (FNUE). In a two-year study (2021 and 2022), the FNUE of onions was determined at five stages of development (at transplant, vegetative growth, bulb initiation, bulb swelling and bulb maturation). The FNUE was estimated by substituting a conventional N fertilizer (ammonium nitrate) with a 5% enriched 15N ammonium nitrate at a rate of 22.4 kg·ha−1 N, at one of five application times corresponding to a stage of development. All onions received a season total of 112 kg·ha−1 N. Marketable yield of onions was significantly greater in 2022 compared to 2021 and FNUE was affected by application timing in both years. In 2021, the FNUE at transplant was 8.9%, increasing to 26.4% and 35.28% at vegetative growth and bulb initiation stages, respectively. At bulb swelling and bulb maturation stages, FNUE was greater than 95%. In 2022, the FNUE at transplant was 25.2%. This increased to 75.7% and 103% at vegetative growth and bulb initiation stages, respectively. Results suggest that the application of fertilizer N at transplant is inefficient due to limited plant uptake ability, while N applications during bulb initiation and swelling were the most efficient.

Differences in the Ultrastructure of Neurons in the Spinal Ganglion and Dorsal Rootlet Between Rats Treated with Cisplatin Only versus Co-administration with a Sphingosine 1-Phosphate Receptor 2 Agonist in Attenuating Neuropathy and Allodynia.

Cisplatin is a chemotherapeutic agent for many types of cancer. The neurotoxicity of cisplatin includes neuropathy and allodynia. We aimed to study structural changes by using CYM54-78, attenuates cisplatin-induced neuropathy and blocks the pathogenesis in neurons and promotes axonal regeneration. TEM (Transmission electron microscopy) was used to distinguish ultrastructural changes in dorsal root ganglion (DRG) and dorsal rootlets (DR) between rats treated with cisplatin alone and rats co-treated with cisplatin and sphingosine -1-phosphate receptor2 (S1P2) agonist, CYM-5478. In DRG of rats treated with cisplatin alone, TEM micrographs showed necrosis and apoptotic cells. Neuronal cytoplasm showed numerous vacuole (stage C) and swelling (stage B➔C) mitochondrial degeneration. Neurons in DRG from cisplatin+CYM-5478 group showed a higher percentage of healthy mitochondria (from 5.3% to 75.6%) than those treated with cisplatin alone. DR of cisplatin only group showed abnormal axoplasm, axolemma and focal detached myelin sheaths, especially in Aδ (fast pain) and Aβ (touch) fibers and revealed collateral branches that sprouted from Aβ fibers, which is characteristic of allodynia. Moreover, vasoconstriction was observed in DRG and DR. Rats in cisplatin+CYM-5478 group showed not only fewer abnormal structures than those in cisplatin only group, but also showed Bands of Büngner and onion bulb-like structures, which are characteristic of nerve regeneration. Together with our previous study, showed that CYM-5478 attenuated neuropathy and allodynia in a rat model of cisplatin-induced neuropathy, these results suggest S1P2 agonists as a potential approach for treatment of cancer due to reduction of side-effects of cisplatin. This article is protected by copyright. All rights reserved.

Pre-bloom control of pear psylla (Cacopsylla pyricola) using chemical compounds in combination with yellow sticky traps

Cacopsylla pyricola (Foerster) (Hemiptera: Psyllidae) is an important pest of pear in Iran. This study was carried out to evaluate the combination of using chemical compounds with yellow sticky traps for control of the pest at pre-bloom stage of pear trees. The experiments were performed using three treatments (2% oil and sticky traps, 1% oil plus Pyriproxyfen and sticky traps, and 1% oil plus fenoxycarb-lufenuron and sticky traps) along with the untreated control in a randomized complete block design. Treatment spraying was performed at bud swelling stage of pear trees, and then four yellow sticky traps were placed on tree branches in all treatments but the control. All the treatments were replicated three times. The mortality data were subjected to one-way ANOVA. The results showed combination of the tested compounds and yellow sticky traps could reduce the pest population up to 99.8%. The findings indicated that 2% oil in combination with sticky traps is very effective in controlling psylla.

Effects of MWCNTs content and dry density on hydro-structural characteristics of bentonite during drying and wetting

Bentonite has been an essential component of geosynthetic clay liners due to its low hydraulic conductivity, which has been used as a barrier against hazardous pollutants in landfills. During the drying and wetting processes caused by high temperatures and leachate in landfills, the hydro-structural characteristics of bentonite vary as the moisture content change. This article investigates the effect of dry density and multi-walled carbon nanotubes (MWCNTs) content on the soil shrinkage characteristic curve (SSCC), the soil free-swelling characteristic curve (SFSCC), the soil water characteristic curve (SWCC), and microstructure of bentonite during the drying and wetting processes. Experimental results indicate that the addition of MWCNTs can slow down the volume variation of bentonite at the normal shrinkage stage and at the initial swelling stage and improve the water retention capacity in the low suction range. Meanwhile, reducing bentonite’s dry density can increase the void ratio during drying, decrease the void ratio during wetting, and improve the water retention capacity in the low suction range. Dry density and MWCNTs content both have no influence on the SSCC at the residual shrinkage stage and the zero shrinkage stage, the SFSCC at the residual swelling stage, and the SWCC at the high suction range.

Gibberellin 2-Oxidases in Potato (Solanum tuberosum L.): Cloning, Characterization, In Silico Analysis and Molecular Docking.

Gibberellins (GAs; tetracyclic di-terpenoid carboxylic acids) are endogenous plant growth regulators responsible for stimulating plant growth and development from seed germination to plant maturity. In potato (Solanum tuberosum L.), GA levels are known to be crucial in the complex process of tuberization. Gibberellin 2-oxidases (GA2oxs) inactivate bioactive GAs during stolon swelling and early stages of tuberization as evident from the predominant expression of a member of this gene family namely GA2ox1. We isolated and characterized a 1105-bp cDNA clone encoding a 340-aa GA2ox1 form, designated St-GA2ox1, using total RNA from growing tuber of a potato (Solanum tuberosum L.) cultivar, Kufri Chipsona-1 (KC-1) based on RT-PCR approach. A total of 26 GA2ox sequences were also retrieved from potato genome database and analysed. Multiple sequence alignment revealed sequence relatedness between the GA2oxs. Crucial protein motifs were identified. Phylogenetic analysis revealed the evolutionary relationships between the GA2oxs. Three-dimensional structure of St-GA2ox1 was predicted by using AlphaFold tool, validated by the predicted local-distance difference test and Ramachandran Plot. Structural analysis and molecular docking were carried out to identify domains, binding sites and affinity for the ligand. The STRING database and hydropathy analysis revealed the presence of a putative interaction site for other enzymes. Expression Atlas database and semi-quantitative RT-PCR revealed the expression patterns of various GA2ox forms in different potato organs. This comprehensive report would be useful in providing new insights into possible underlying mechanisms involved in tuber development, and could facilitate the targeted alteration of genes responsible to combat the stress and enhance tuber production.

Effects of Slow-Release Fertilizer on Lotus Rhizome Yield and Starch Quality under Different Fertilization Periods

Slow-release fertilizer is an environmentally friendly fertilizer that is widely used in crop cultivation instead of traditional nitrogen fertilizer. However, the optimal application time of slow-release fertilizer and its effect on starch accumulation and rhizome quality of lotus remains unclear. In this study, two slow-release fertilizer applications (sulfur-coated compound fertilizer, SCU, and resin-coated urea, RCU) were fertilized under three fertilization periods (the erect leaf stage, SCU1 and RCU1; the erect leaf completely covering the water stage, SCU2 and RCU2; and the swelling stage of lotus rhizomes, SCU3 and RCU3) to study the effects of different application periods. Compared with CK (0 kg∙ha-1 nitrogen fertilizer), leaf relative chlorophyll content (SPAD) and net photosynthetic rate (Pn) remained at higher levels under SCU1 and RCU1. Further studies showed that SCU1 and RCU1 increased yield, amylose content, amylopectin and total starch, and the number of starch particles in lotus, and also significantly reduced peak viscosity, final viscosity and setback viscosity of lotus rhizome starch. To account for these changes, we measured the activity of key enzymes in starch synthesis and the relative expression levels of related genes. Through analysis, we found that these parameters increased significantly under SCU and RCU treatment, especially under SCU1 and RCU1 treatment. The results of this study showed that the one-time application at the erect leaf stage (SCU1 and RCU1) could improve the physicochemical properties of starch by regulating the key enzymes and related genes of starch synthesis, thus improving the nutritional quality of lotus rhizome. These results provide a technical choice for the one-time application of slow-release fertilizer in lotus rhizome production and cultivation.

Settlement of a swelling plastic particle in the hydrothermal environment

The knowledge of particle motion is vital for improving the conversion efficiency of hydrothermal reactors. Compared with evaporating or burning particles at gas ambient, the distinct thermophysical properties of the liquid fluid are expected to affect the motion of reactive particles. This work conducts an experimental study on the settlement of a plastic particle in hydrothermal conditions. A static hydrothermal process of particles is first performed to clarify particle morphology changes. Subsequently, in the dynamic settlement, all experimental particles are determined to be at a swelling stage, where the particle size increases by absorbing the fluid. The falling trajectory of particles shows an irregular spatial curve, and the eccentric position of the particle during the settlement also affects its size. Finally, the thermophysical properties of fluid and particle are also estimated based on the static experiment to predict the drag coefficient of falling particles. The results show that the drag coefficient of swelling particles in the tube is significantly larger than that of rigid particles, and the drag coefficient gradually decreases with the Reynolds number, especially in the case of small particles.