Life Stages Research Articles

Lactation, mastitis, and probiotics.

Exclusive breastfeeding during the first six months of life, and prolonged with complementary feeding until 2 years of age or more, is the best nutritional approach in the first stage of life. Mastitis is a pathology that can jeopardize the continuity of breastfeeding. Different methods have been proposed for the prevention and treatment of mastitis, including probiotics. This narrative review aims to describe the problem of mastitis during lactation and the role of probiotics in its prevention. Mastitis should not be confused with the physiological phenomenon known as milk coming in. In persistent pain, timely medical consultation is key to avoiding complications. The administration of specific probiotics in the perinatal period has shown efficacy in reducing the incidence of mastitis.

Aging Anxiety, Identity and Gender Equality: A Study on Key Issues in Contemporary Western Gerontology

Since the late 20th century, Western gerontological research has expanded from purely biomedical to a broader cultural approach, developing interdisciplinary subfields such as humanistic and cultural gerontology. Cultural gerontology, drawing from cultural studies theories, places aging in parallel with categories like class, gender, and race to explore its culturally constructed attributes. Traditionally, old age has been negatively stereotyped as fragile and obstinate, leading to societal fear and discrimination. In reality, the segmentation of life stages is a product of societal evolution. Analyzing aging through a cultural studies lens—focusing on identity, aging anxiety, and gender power dynamics—reveals the critical role of culture in shaping the experiences and identities of the elderly. This approach effectively deconstructs negative stereotypes associated with old age and provides insights for the development of gerontology in China.

Single-Larva RNA Sequencing Reveals That Red Sea Urchin Larvae Are Vulnerable to Co-Occurring Ocean Acidification and Hypoxia.

Anthropogenic carbon dioxide emissions have been increasing rapidly in recent years, driving pH and oxygen levels to record low concentrations in the oceans. Eastern boundary upwelling systems such as the California Current System (CCS) experience exacerbated ocean acidification and hypoxia (OAH) due to the physical and chemical properties of the transported deeper waters. Research efforts have significantly increased in recent years to investigate the deleterious effects of climate change on marine species, but have not focused on the impacts of simultaneous OAH stressor exposure. Additionally, few studies have explored the physiological impacts of these environmental stressors on the earliest life stages, which are more vulnerable and represent natural population bottlenecks in organismal life cycles. The physiological response of the ecologically and commercially important red sea urchin (Mesocentrotus franciscanus) was assessed by exposing larvae to a variety of OAH conditions, mimicking the range of ecologically relevant conditions encountered currently and in the near future along the CCS. Skeleton dissolution, larval development, and gene expression show a response with clearly delineated thresholds that were related to OAH severity. Skeletal dissolution and the induction of Acid-sensing Ion Channel 1A at pH 7.94/5.70 DO mg/L provide particularly sensitive markers of OAH, with dramatic shifts in larval morphology and gene expression detected at the pH/DO transition of 7.71/3.71-7.27/2.72 mg/L. Experimental simulations that describe physiological thresholds and establish molecular markers of OAH exposure will provide fishery management with the tools to predict patterns of larval recruitment and forecast population dynamics.

A loop‐mediated isothermal amplification assay to detect insect‐associated Serratia spp. in the critically endangered insect Dryococelus australis

Abstract Point‐of‐care diagnostic methods are being increasingly employed to monitor infection risks in animal populations. These approaches are particularly valuable for ex situ invertebrate conservation programmes that often involve large numbers of individuals and where the rapid return of results supports timely risk mitigation decisions. Insect frass is a useful substrate to screen for the presence of pathogens transmitted by the faecal‐oral route in live insects but can result in false negatives when using common nucleic acid detection techniques, such as polymerase chain reaction (PCR), because of poor DNA extraction yields and the presence of inhibitors. We developed a diagnostic test to detect potentially entomopathogenic Serratia spp. in the frass of captive Dryococelus australis, using a cleanup step to improve DNA extraction by separating and concentrating bacterial cells, and a loop‐mediated isothermal amplification (LAMP) assay targeting the ureD gene. The LAMP assay had 1000 times greater analytical sensitivity than quantitative PCR when testing frass, did not yield false positive reactions when used to test a panel of 102 non‐Serratia spp., achieved results in 30 min and required only basic equipment. We applied the assay in real‐life conditions to screen captive insects, yielding information about the enclosures, life stages and insect species shedding potential entomopathogens, to inform the biosecurity and management practices of the captive breeding and conservation programme for D. australis. This diagnostic test could be modified for use in field conditions and adapted to detect other pathogens of concern in invertebrate conservation programmes and for insects reared in captivity for research or agricultural purposes.

Framing and Management of Migraines in Women: An Expert Opinion on Challenges, Current Approaches, and Future Multidisciplinary Perspectives

Background/Objectives: Migraines are a common neurological disorder that significantly impact women, especially during their reproductive years. Hormonal, neurological, and lifestyle factors shape migraine patterns, with fluctuations during menstruation, pregnancy, perimenopause, and menopause influencing migraine prevalence and severity. This expert opinion explores current challenges, therapeutic strategies, and future directions for personalized care, addressing the limited inclusion of women in clinical research across different life stages. Methods: In order to focus on hormonal influences, pharmacological and non-pharmacological therapies, including CGRP monoclonal antibodies, neuromodulation, and lifestyle interventions, a comprehensive analysis of literature, in particular on clinical trials, real-world studies, and guidelines on migraine management was performed. Emerging digital tools and AI-based approaches were also evaluated to improve personalized care for women with migraine. Results: Hormonal therapies, including contraceptives and HRTs, present both risks and benefits, particularly for women with migraines with aura, highlighting the need for individualized approaches. Advances in CGRP-targeted therapies have shown effectiveness in preventing refractory migraines. Non-pharmacological treatments, such as neuromodulation, acupuncture, and lifestyle adjustments, further expand the treatment landscape. However, research gaps remain, particularly regarding hormonal influences on migraines during pregnancy and menopause. Conclusions: Future research should prioritize female-specific clinical trials to better understand the impact of hormonal changes on migraines. Tailored therapies combining pharmacological, non-pharmacological, and digital solutions are essential for improving care. A multidisciplinary approach integrating personalized medicine, technological advancements, and patient education is crucial to optimizing outcomes and enhancing quality of life for women with migraine.

AEDES JAPONICUS: A TENANT INVADER IN THE GREAT SMOKY MOUNTAINS NATIONAL PARK, USA1.

Invasive organisms may cause ecologic, economic, and public health harm. Aedes japonicus is an invasive mosquito species of known ecologic and public health importance that has widely spread throughout the eastern USA since initially being recognized in Connecticut in 1998. Here, we report the known distributions of Ae. japonicus within the Great Smoky Mountains National Park (GSMNP) since its initial recognition in the park in 2004. From 2006 to 2022, we sampled eggs, larvae, and adult life stages through targeted, haphazard, and convenience collections. Through these efforts, we surveyed 23 (54.7%) of the 42 watersheds within the GSMNP. Aedes japonicus was present in 19 (82.6%) of the sampled watersheds, and the species was confirmed, in some instances, at the same location over multiple years, suggesting it remains entrenched. This species was observed in 45.2% of the GSMNP watersheds at elevations ranging from 347 to 1,478 m. Naturally occurring containers (i.e., riverine rock pools) were common collection sites in this study. The results of our findings are presented in the context of the species distribution within the park, the public health relevance given the GSMNP's public visitation rate (>12 million annually), potential species interactions, and the persistence of this species over the multiyear study.

Influence of Light Intensity and Temperature on the Development of Early Life Stages of Ascophyllum nodosum (Phaeophyceae)

Ascophyllum nodosum is an ecologically and economically important species forming marine forests in temperate regions. In Europe, this brown seaweed reaches its southern distribution limit in the north of Portugal, where populations are under climatic pressure. Conservation and restoration actions are essential to preserve the important ecological roles of these populations, including biodiversity enhancement. In this study, we assessed the effect of temperature and light on the development of the early life stages of A. nodosum, from gamete germination and rhizoid development to germling growth, in order to support the establishment of nursery protocols for producing seedlings that can be used in reforestation actions. We found that for this population, temperature around 12 °C and low-light conditions (40 μmol m−2 s−1) favor gamete germination, rhizoid development, and initial germling growth during the first month, after which higher light supply (>80 μmol m−2 s−1) is needed to sustain further growth stages. The results obtained in this study are relevant for the establishment of nursery methods for A. nodosum and should be complemented by further studies to determine more precisely the light and nutrient requirements to optimize growth in the germlings’ later growth stages.

Discovery of a potential open ocean nursery for the endangered shortfin mako shark in a global fishing hotspot

Populations of large pelagic sharks are declining worldwide due to overfishing. Determining the overlap between shark populations and fishing activities is important to inform conservation measures. However, for many threatened sharks the whereabouts of particularly vulnerable life-history stages – such as pregnant females and juveniles – are poorly known. Here, we investigated the spatial distribution of size classes, energy transfer and reproductive states of pregnant females of the endangered shortfin mako, Isurus oxyrinchus, using spatially resolved catch data from a Spanish surface longline vessel (1996 − 2009) in the South-east Pacific Ocean. Our results suggest a general eastward gradient of occurrence of pregnant females of thousands of kilometers from western oceanic feeding grounds towards the eastern Pacific, where we observed an aggregation area of small juveniles. Moreover, the potential nursery likely overlapped a longline fishing hotspot, increasing the vulnerability of juveniles from fisheries. Our results suggest that limiting fishing pressure in this area could reduce mortality of early life stages and contribute to the conservation of this endangered shark species.

The lateralized cerebellum: insights into motor, cognitive, and affective functioning across ages: a scoping review.

Research on the cerebellum and its functional organization has significantly expanded over the last decades, expanding our comprehension of its role far beyond motor control, including critical contributions to cognition and affective processing. Notably, the cerebellar lateralization mirrors contralateral brain lateralization, a complex phenomenon that remains unexplored, especially across different stages of life. The present work aims to bridge this gap by providing a comprehensive scoping review of the lateralization of motor, cognitive, and affective functioning within the cerebellum across the lifespan. A methodical search in electronic databases (i.e., PubMed, Embase, and PsycINFO) was conducted up to October 2024, focusing on neuroimaging studies with healthy participants of all ages performing motor, cognitive, or affective tasks. Our selection process, which involved multiple independent reviewers, identified 128 studies reporting cerebellar asymmetries in individuals from early childhood to older age, with a significant portion of studies regarding young-middle adults (19-45years old). The majority of the findings confirmed established lateralization patterns in motor and language processing, such as ipsilateral motor control and right-lateralized language functions. However, less attention has been paid to other cognitive functions and affective processing where more heterogeneous and less consistent asymmetries have been observed. To the best of our knowledge, this scoping review is the first to comprehensively investigate the motor, cognitive, and affective functional lateralization of the cerebellum across lifespan, highlighting previously overlooked dimensions of cerebellar contributions.

Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies

The remarkable diversity of insect pigmentation offers a captivating avenue for studying evolution and genetics. In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. Here, we present evidence from classical and modern genetics showing that the black pupae (bp) phenotype in the GUA10 strain of Anastrepha ludens is caused by a large deletion at the ebony locus, removing the gene’s entire coding region. Targeted knockout of ebony induced analogous bp phenotypes across six major tephritid agricultural pests, demonstrating that disruption of Ebony alone is sufficient to produce the mutant trait in distantly related species. This functional characterization further allowed a deeper exploration of Ebony’s role in pigmentation and development across life stages in diverse species. Our findings offer key insights for molecular engineering of sexing strains based on the bp marker and for future evolutionary developmental biology studies in tephritids.

A Real-Time PCR Assay for Detecting Codling Moth Cydia pomonella on Material Intercepted at U.S. Ports of Entry—A Valuable Tool for Specimen Identification

Codling moth Cydia pomonella is well established nearly everywhere apples are grown. Due to this almost global distribution, larvae are often intercepted at U.S. ports of entry where immature specimens cannot be identified accurately to species leading to unnecessary quarantine actions. To assist with identifying intercepted C. pomonella from port inspections, we developed a probe-based real-time PCR assay to amplify the internal transcribed spacer (ITS) region 2 of C. pomonella. The assay was tested for inclusivity using 110 C. pomonella specimens from six continents. Analytical specificity was examined by testing related species intercepted at U.S. ports of entry, as well as non-targets with the same geographic distribution and host species as C. pomonella. The assay developed here identified all C. pomonella individuals correctly and produced appropriately negative results for all non-target species. These results ensure that the assay provides a rapid and accurate tool for unambiguously identifying C. pomonella among material intercepted at U.S. ports of entry. Since C. pomonella is not actionable, the ability to identify all life stages of C. pomonella conclusively will save time, effort, and money while also directing identification efforts towards species of current quarantine concern.

Macular, choroidal and disc associations across women's reproductive life stages: a scoping review from menarche to post-menopause.

Oestrogen and progesterone fluctuate cyclically in women throughout their adult lives. Although these hormones cross the blood-retinal barrier and bind to intraocular receptors, their effects remain unclear. We present the first review to date on associations between posterior pole structures-specifically the macula, choroid, and optic disc-and boththe menstrual cycle and post-menopausal period, utilising multimodal imaging techniques in healthy adult non-pregnant women. We excluded studies on contraception and hormonal replacement therapy, focusing solely on physiological associations. Despite the comprehensive scope of our review, limited data and inconsistent reporting among studies prevented the establishment of meaningful trends. Across menstrual cycle phases, choroidal thickness (CHT) was the most consistently reported parameter, with thinning during the luteal phase compared to the follicular phase. Conversely, no significant differences were observed in macular or disc morphology across the cycle, likely reflecting a preserved structure despite potential fluctuations in blood flow and perfusion. Studies comparing pre- and post-menopausal associations, after adjusting for age or body mass index (BMI), failed to reveal meaningful trends, highlighting the difficulty in separating the effect of age from hormonal declines in older women. Understanding how hormonal cycles impact the posterior pole in women is crucial for addressing sex differences in various ocular pathologies. Research on female-specific factors is still sparse, and interestingly, the majority of affiliations in the reviewed articles did not originate from regions with the highest biomedical research funding and publication rates. We encourage further studies focusing on female-specific variables and provide recommendations for future designs.

Influence of social media on children's dietary habits

Introduction: Dietary habits refer to the consistent choices made by individuals or groups concerning their food consumption. It has a crucial influence in human health and reflects individual eating choices, which are often associated with culture, education, social background, and health status. Dietary habits may be influenced and altered by life stages and lifestyle factors, including physical activity and social interac-tion. Nutritional consumption significantly influences human health and well-being, particularly during childhood and adolescence, and directly impacts children's health due to their physical, mental, and cognitive development. Approaches: Data has been gathered from 100 children to evaluate the impact of social media on their dietary practices. The questionnaire was administered to professionals to establish its validity and subsequently its reliability. The total number of items in the questionnaire was 19. To evaluate children's behavior on social media about eating practices. The data was gathered by a simple random sampling process and analyzed using both descriptive and inferen-tial statistical techniques. Outcomes The results indicated that among 100 children, the mean age of participation was 15.66 years. The cur-rent study demonstrated a moderate impact of social media on children's food habits. The study demonstrated a substantial statistical link between children's age and hours spent on social media, as well as the impact of social media on children's food habits (p < 0.05). The re-sults indicated no statistically significant differences (p > 0.05) between social media usage and other demographic variables. Concludes The findings indicated that social media exerted a moderate influence on children's food habits. The study concluded that a substantial statistical link exists between children's age, hours of social media usage, and the impact of social media on children's food habits. The results indicat-ed no significant statistical differences between social media usage and other demographic variables.

The Neurologist's Role in Promoting Brain Health: Emerging Issues in Neurology.

Neurologic conditions are a leading cause of morbidity and mortality within the United States and worldwide. Brain health is a global concern, and the American Academy of Neurology's Brain Health Initiative promises to drive progress in this field over the next decades. Neurologists with detailed training and insight into brain function are uniquely positioned to apply emerging preventive health data to promote healthy brain development and maintain optimal brain function throughout the lifespan. The neurologist's role in promoting brain health is also vital in patients with active neurologic disease, in whom preventive measures may reduce recurrence or slow progression of disease and may enhance quality of life and overall function. In this Emerging Issues in Neurology article, we present the factors that may protect brain function and frame a practical approach to screening assessments and preventive interventions that neurology clinicians may consider to improve the brain health of patients at all life stages.

BMI trajectories are associated with NAFLD and advanced fibrosis via aging-inflammation mediation

BackgroundAs the global epidemic of obesity fuels metabolic conditions, the burden of nonalcoholic fatty liver disease (NAFLD) will become enormous. Abundant studies revealed the association between high body mass index (BMI) and NAFLD but overlooked the BMI patterns across life stages. We aimed to explore how BMI trajectories over age relate to NAFLD.MethodsSelecting 3212 participants in NHANES 2017–2020, we tracked BMI records at different ages. Using a latent class trajectory model (LCTM), we identified BMI trajectories over age. Multinomial logistic regression assessed their association with NAFLD and advanced fibrosis. Structural equation modeling (SEM) revealed mediation effects.ResultsWe identified 3 BMI trajectories: Steady Progression, Increase to Decrease, and Rapid Ascending. There was no significant difference in NAFLD/advanced fibrosis risk between the increase-to-decrease group and the steady progression group. The Rapid Ascending trajectory significantly correlated with NAFLD (OR = 2.21, 95% CI 1.29–3.77) and advanced fibrosis (OR = 3.04, 95% CI 1.13–8.22). This association was influenced by a chain-mediated process of phenotypic age and C-reactive protein (mediated effect to NAFLD = 0.010, p < 0.01; mediated effect to advanced fibrosis = 0.003, p < 0.05). This mediation on NAFLD was independent of insulin resistance (IR). The association between rapid ascending trajectory and advanced fibrosis was more pronounced among the male subgroup (p for interaction = 0.008).ConclusionThe rapid ascending trajectory of BMI correlates with an increased susceptibility to NAFLD and advanced fibrosis independent of BMI, mediated by aging and inflammation. Our results suggest that long-term maintenance of BMI is pivotal in NAFLD prevention. Aging-inflammation may represent a distinct mechanism of sustained obesity to NAFLD, independent of IR.

Targeting T-Cell Activation for Malaria Immunotherapy: Scoping Review

Malaria remains a critical global health issue due to high mortality rates, drug resistance, and low treatment efficacy. The genetic variability of Plasmodium proteins complicates the development of long-lasting immunity, as it impedes the human immune system’s ability to sustain effective responses. T cells play a crucial role in combating malaria, but the parasite’s complex life cycle—spanning liver and blood stages—presents significant challenges in effectively activating and targeting these cells. Immunotherapy, which enhances the immune response and promotes durable T cell activity, offers a promising avenue for more effective and lasting malaria treatments. This review systematically analyzed 63 studies published in the last decade, focusing on the role of T cells in malaria. Among the studies, 87.2% targeted T cells as immunotherapy candidates, with CD4+ and CD8+ T cells each accounting for 47.6% of the studies. γδ T cells were the focus in 7.9% of cases, while 12.7% explored non-T cell contributions to enhancing T cell-mediated responses. The findings underscore the potential of T cells, particularly CD8+ T cells, in liver-stage defense and advocate for the exploration of advanced vaccine platforms and novel therapies, such as mRNA-based vectors and monoclonal antibodies.

A comparative study of the genetically improved Abbassa Nile tilapia strain (GIANT-G9) and a commercial strain in Egypt: growth vs. stress response

Selective breeding is a potent method for developing strains with enhanced traits. This study compared the growth performance and stress responses of the genetically improved Abbassa Nile tilapia strain (G9; GIANT-G9) with a local commercial strain over 12 weeks, followed by exposure to stressors including high ammonia (10 mg TAN/L), elevated temperature (37 °C), and both for three days. The GIANT-G9 showed superior growth, including greater weight gain, final weight, length gain, specific growth rate, and protein efficiency ratio, as well as a lower feed conversion ratio and condition factor compared to the commercial strain. The expression of growth hormone in the brain of the GIANT‐G9 increased significantly after 6 weeks, although it slightly decreased after 12 weeks. Growth hormone receptor 1 expression also increased significantly after 6 weeks. Muscle insulin-like growth factors (igf1 and igf2) levels up-regulated significantly only after 12 weeks in the GIANT‐G9. Under stress, serum enzymes (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase (ALP)) were significantly higher in the GIANT‐G9, while the commercial strain had lower levels. No significant changes were observed in liver ALP activity among stressed strains. Under stress, the GIANT‐G9 exhibited marked upregulation of splenic Toll-like receptors (tlr2, tlr9, tlr21), myeloid differentiation primary response protein 88 (myd88), nuclear factor kappa B (nf-κB), interleukin (il) 1β, and il6. Notably, il6 expression was higher than il1β in the spleen, with the opposite pattern in the head kidney. In response to immune stimulation, globulin levels significantly increased in the GIANT‐G9 but with similar values to the stressed commercial strain. Myostatin expression increased in the spleen of the stressed GIANT‐G9. The commercial strain exhibited the best liver catalase and superoxide dismutase activities under stress, while the GIANT‐G9 showed increased liver glutathione-S-transferase (GST) activity after exposure to ammonia and temperature stress. Serum lysozyme activity increased in the stressed commercial strain and under temperature stress in the GIANT‐G9 but decreased under other stress conditions. Overall, the stressed commercial strain demonstrated higher survivability than the stressed GIANT‐G9. The study revealed significant interactions between strains and stress factors. The GIANT‐G9 exhibited higher growth rates but lower antioxidant and immune capacities compared to the commercial strain at the juvenile stage of life and production cycle.

Nutritional carryover effects of the previous plane of nutrition of crossbred Angus steers affects freshwater intake, animal performance, and water and feed efficiency

Abstract This study evaluated how the backgrounding diet can affect water intake, animal performance and water and feed efficiency of steers fed finishing diets based on grains or forages. Twenty-four crossbred Angus steers (298 ±10.2 kg) were fed during backgrounding either a moderate (MP, n =12; triticale hay only, 9.1 %crude protein [CP], 1.07 Mcal/kg net energy available for maintenance [NEm], and 0.52 Mcal/kg net energy available for gain [NEg]) or high plane of nutrition (HP, n =12; 85% alfalfa hay and 15% beardless wheat hay, 12.62 %CP, 1.23 Mcal/kg NEm, and 0.66 Mcal/kg NEg) during the background phase (85 d). After this period, steers were assigned to two finishing phase groups containing half of the animals from each backgrounding diets. During the finishing phase, animals were fed either a forage-based (Alfalfa hay; CP: 20.8% DM, NEm: 1.47 Mcal/kg, NEg: 0.88 Mcal/kg) or a grain-based diet (80% whole corn and 20% wheat hay; CP: 10.6% DM, NEm: 1.96 Mcal/kg, NEg: 1.31 Mcal/kg). There was no interaction between the effects of the previous plane of nutrition [(moderate (MP) or high (HP)] and finishing diets (forage or grain-fed) on any of the variables studied (P &amp;gt; 0.05). Animals backgrounded on MP were lighter and had a lower DMI when compared with HP (P &amp;lt; 0.01). However, no differences were observed on the drinking water intake (DWI) between MP and HP (P &amp;gt; 0.05). HP animals were more efficient in gross water efficiency (P &amp;lt; 0.01), but less efficient on residual feed intake (P &amp;lt; 0.05), compared to MP animals. During the finishing phase, forage fed animals had a greater DMI and DWI compared with grain-fed animals (P &amp;lt; 0.01), however, no differences were observed on the final BW (P &amp;gt; 0.05). Grain-fed animals were more efficient for water and feed intake and had a greater marbling score (P &amp;lt; 0.01) compared to forage-fed. Animals fed on HP and finished in a grain-fed diet had the least DWI (P &amp;lt;0.05) and greatest body condition score (P &amp;lt; 0.05), marbling score (P &amp;lt; 0.01) and rib depth (P &amp;lt; 0.05). In the first 20 days of the finishing period, the previous plane affected the variables ADG, DMI, CPi, and DWI (as kg DMI and CP) in the finishing plane. Altogether, our results highlight the individual effects of backgrounding and finishing systems on DWI and the efficiency of finishing animals, as well as the importance of providing adequate nutrition during the earlier stages of life.

SPLASH Into Fitness: A Swimming-Based Pilot Intervention to Promote Healthful Identities and Behaviors Among Preadolescent Girls.

Girls' physical activity and healthful eating behaviors decline throughout adolescence. These trajectories may be linked to the underdevelopment of exercise and healthful eater identities. Youth programs might consider prioritizing identity development as an innovative health promotion strategy, especially during the formative preadolescent life stage. This study examines healthful identities and behaviors among a sample of preadolescent girls who participated in SPLASH, a multicomponent youth program consisting of identity development ("I am an exerciser"; "I am a healthy eater"), physical activity, and healthful eating. SPLASH was piloted in a community-based recreational facility in the Midwest United States. Participants had differing levels of program access: all girls (N=32, age=10.6±0.8) attended a 1-week summer day camp, and a subset (n=16) received supplementary access to a 10-week eHealth program. Using a feasibility framework, we examined SPLASH's (1) preliminary efficacy, (2) implementation, and (3) acceptability. Girls' exercise identities and behaviors improved following camp participation. At follow-up, those in the camp-plus-eHealth group reported greater improvements in physical activity enjoyment relative to girls in the camp-only group. SPLASH is a promising identity-focused health promotion program for preadolescent girls. Community support and family engagement facilitated this pilot study. Programmatic impact should be assessed in additional communities.

Impact of Feeding Frequency on Growth Performance and Antioxidant Capacity of Litopenaeus vannamei in Recirculating Aquaculture Systems

Feeding frequency is crucial for the growth and development of white shrimp (Litopenaeus vannamei) at various life stages. Although higher feeding frequencies can enhance growth, manual feeding methods significantly increase labor costs. Automatic feeding systems offer a cost-effective and efficient alternative, yet their application in intensive shrimp culture remains under-researched. This study evaluates different feeding frequencies for L. vannamei in intensive aquaculture tanks, focusing on growth performance, survivability, feed utilization, digestive and antioxidant capacities, and economic viability. Juvenile shrimp (3.85 ± 0.3 g) were cultured for 63 days with feeding frequencies of 6, 8, 10, and 12 times/day (A6, A8, A10, and A12 groups, respectively) using automatic feeders, with a control group fed manually 6 times/day (M6). Results indicated that automatic feeding significantly improved final body weight, specific growth rate, and feed conversion ratio compared to manual feeding. Among automatic feeding groups, A6 and A8 showed optimal performance, with a quadratic regression identifying 7.83 times/day as the optimal frequency. While digestive enzyme activity remained consistent across groups, A6 and A8 demonstrated significantly higher antioxidant enzyme levels (superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and lower lipid peroxidation (MDA), suggesting superior digestive and antioxidant capacities. Pearson’s correlation confirmed a positive relationship between SOD and GPx. Economically, the A8 group achieved the highest profitability. Consequently, a feeding frequency of 6–8 times/day using automatic feeders is recommended as an optimal and feasible strategy for intensive white shrimp culture in this life stage.