Strontium isotope studies (120 analyses) were carried out on ore and gangue minerals from five ore districts in different carbonate basins. Most of the samples were separated from consecutive diagenetic generations. Even for diverse stratigraphic positions and mineralogies, very consistent trends are recognized. In all cases87Sr/86Sr ratios increase with advancing diagenetic stage. However, each district shows a different behaviour in the magnitude of increase over the first diagenetic generation, which ideally is close to contemporaneous seawater: The increase of the strontium ratios in the subsequent diagenetic generations is explained by equilibration during crystallization with fluids evolving towards more radiogenic compositions. This is interpreted as a consequence of the reaction of the fluids with Rb-bearing phases, mainly detrital clay minerals. Variation ranges of87Sr/86Sr ratios can constitute a sensitive tool for determining the stage of basin evolution in a similar way as vitrinite reflectance or illite crystallinity. This method has the disadvantage that the increase of radiogenic strontium depends not only on the evolution stage, i.e., temperature, pressure, and time, but also on the amount of Rb-bearing minerals present in the basin. On the other hand, this method has the important advantage that within a given basin this last parameter can be modelled. Each evolution stage is recorded in the cements or crystallization generations by its strontium isotope signatures, which tend towards increased radiogenic values with advancing diagenesis.