Stages Of Life Cycle Research Articles

ANALYSIS OF TYPES OF VEHICLE POWER PLANTS: ADVANTAGES AND DISADVANTAGES

This paper analyzes the advantages and disadvantages of vehicle power plants, examines the development and modern types of hybrid power plants used in transport. An analysis of carbon dioxide (CO2) emissions into the atmosphere from vehicles equipped with different types of powertrains, including internal combustion engines (ICE), hybrid drives, and electric motors, was performed. Studies have been conducted of emissions that occur during the operation of vehicles, as well as emissions associated with the production of high-voltage batteries for electric vehicles and the production of electricity required for their charging. It was concluded that electric vehicles, despite their popularity as environmentally friendly vehicles, can have a significant impact on the environment. This is due to high CO2 emissions during the production of batteries, which are a key component of electric vehicles. The production of these batteries requires significant energy costs and the use of various materials, the extraction and processing of which also lead to greenhouse gas emissions. In addition, the environmental impact of electric vehicles largely depends on the sources of electricity used to charge them. When electricity is generated from fossil fuels such as coal or natural gas, overall CO2 emissions can be significantly higher, reducing the environmental advantage of electric vehicles over internal combustion engines. On the other hand, the use of renewable energy sources, such as solar or wind energy, to charge electric vehicles significantly reduces CO2 emissions into the atmosphere. The work also considers hybrid vehicles that combine an internal combustion engine and an electric motor. Such cars usually have lower CO2 emissions during operation compared to traditional diesel vehicles, but their environmental impact also depends on the production and disposal of batteries. To make informed decisions about the choice of powertrain and the development of transport infrastructure, all aspects must be taken into account, including the production of vehicles, their operation and disposal. It is also important to develop technologies to reduce emissions and increase energy efficiency at all stages of a vehicle's life cycle. The work concluded that to reduce the overall impact of vehicles on the environment, it is necessary not only to develop electric vehicles, but also to combine the advantages of internal combustion engines and electric motors (develop a hybrid drive), improve battery production technologies, expand the use of renewable energy sources, and implement other measures to reduce CO2 emissions.

OsMBF1a Facilitates Seed Germination by Regulating Biosynthesis of Gibberellic Acid and Abscisic Acid in Rice.

Seed germination is a pivotal stage in the plant life cycle, orchestrated by a myriad of internal and external factors, notably plant hormones. The underlying molecular mechanisms governing rice seed germination remain largely unelucidated. Herein, we uncover OsMBF1a as a crucial regulatory factor that employs a dual strategy to promote seed germination: positively activating genes involved in gibberellin (GA) biosynthesis pathways, while negatively regulating key genes responsible for abscisic acid (ABA) synthesis. Furthermore, OsMBF1a modulates the endogenous levels of ABA and GA in rice seeds, reinforcing its central role in the germination process. The expression of ZmMBF1a and ZmMBF1b, the homologous genes in maize, in rice seeds similarly affects germination, indicating the conserved functionality of MBF1 family genes in regulating seed germination. This study provides novel insights into the molecular mechanisms underlying rice seed germination and underscores the significance of MBF1 family genes in plant growth and development.

A land plant phylogenetic framework for GLABROUS INFLORESCENCE STEMS (GIS), SUPERMAN, JAGGED and allies plus their TOPLESS co-repressor

Members of the plant specific family of C1-1i zincfinger transcriptionfactors (ZF-TFs), such as SUPERMAN, JAGGED, KNUCKLES or GIS,regulatediversedevelopmental processes including sexual reproduction. C1-1is consist of one zinc-finger and one to two EAR domains, connected by large intrinsically disordered regions (IDR). While the role of C1-i1 ZF-TFs in development processes is well known for some genes in Arabidopsis, rice or tomatoa comprehensive and broadphylogenetic background is lacking, yet knowledge of orthology is a requirement for a better understanding of C1-1i-Zf-TFs diverse roles in plants. Here, we provide a fine-grained and land plant wide classification of C1-1i sub-families and their known co-repressors TOPLESS and TOPLESS RELATED. Our work combines the identification of orthologous groups with Maximum-Likelihood phylogeny reconstructions and digital gene expression analyses mining high quality land plant genomes and transcriptomes to generate a comprehensive framework of C1-1i ZF-TF evolution. We show that C1-1i’s are low to moderate copy genesand that orthologous genesonly partiallyhaveconserved sub-family and life cycle stage dependent expression pattern across land plants while others are highly diverged. Our workprovides the phylogenetic framework for C1-1i ZF-TFs, s and strengthen C1-1 ZF-TFs as a potential model for IDR-research in plants.

Using Satellite Observations of Lightning and Precipitation to Diagnose the Behavior of Deep Convection in Tropical Cyclones Traversing the Midlatitudes

AbstractThis study uses a unique combination of geostationary and low‐Earth orbiting satellite‐based lightning and precipitation observations, respectively, to examine the evolution of deep convection during the tropical cyclone (TC) lifecycle. The study spans the 2018–2021 Atlantic Basin hurricane seasons and is unique as it provides the first known analysis of total lightning (intra‐cloud and cloud‐to‐ground) observed in TCs through their extratropical transition and post‐tropical cyclone (PTC) phases. We consider the TC lifecycle stage, geographic location (e.g., land, coast, and ocean), shear strength, and quadrant relative to the storm motion and environmental shear vectors. Total lightning maxima are found in the forward right quadrant relative to storm motion and downshear of the TC center, consistent with previous studies using mainly cloud‐to‐ground lightning. Increasing environmental shear focuses the lightning maxima to the downshear right quadrant with respect to the shear vector in tropical storm phases. Vertical profiles of radar reflectivity from the Global Precipitation Measurement mission show that super‐electrically active convective precipitation features (>75 flashes) within the PTC phase of TCs have deeper mixed phase depths and higher reflectivity at −10°C than other phases, indicating the presence of more intense convection. Differences in the net convective behavior observed throughout TC evolution manifest in both the TC‐scale frequency of lightning‐producing cells and the intensity variations amongst individual convective cells. The combination of continuous lightning observations and precipitation snapshots improves our understanding of convective‐scale processes in TCs, especially in PTC phases, as they traverse the tropics and mid‐latitudes.

Inorganic Polyphosphate Is in the Surface of Trypanosoma cruzi but Is Not Significantly Secreted.

Trypanosoma cruzi is the etiologic agent of Chagas disease, an infection that can lead to the development of cardiac fibrosis, which is characterized by the deposition of extracellular matrix (ECM) components in the interstitial region of the myocardium. The parasite itself can induce myofibroblast differentiation of cardiac fibroblast in vitro, leading to increased expression of ECM. Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate that can also induce myofibroblast differentiation and deposition of ECM components and is highly abundant in T. cruzi. PolyP can modify proteins post-translationally by non-enzymatic polyphosphorylation of lysine residues of poly-acidic, serine-(S) and lysine (K)-rich (PASK) motifs. In this work, we used a bioinformatics screen and identified the presence of PASK domains in several surface proteins of T. cruzi. We also detected polyP in the external surface of its different life cycle stages and confirmed the stimulation of host cell fibrosis by trypomastigote infection. However, we were not able to detect significant secretion of the polymer or activation of transforming growth factor beta (TGF-β), an important factor for the generation of fibrosis by inorganic polyP- or trypomastigote-conditioned medium.

Prevalence of human schistosomiasis in various regions of Tanzania Mainland and Zanzibar: A systematic review and meta-analysis of studies conducted for the past ten years (2013-2023).

Schistosomiasis is a significant public health problem in Tanzania, particularly for the people living in the marginalized settings. We have conducted a systematic review with meta-analysis on the prevalence of schistosomiasis to add knowledge towards the development of effective approaches to control the disease in Tanzania. Online databases namely, Pub Med, SCOPUS and AJOL, were systematically searched and a random effect model was used to calculate the pooled prevalence of the disease. Heterogeneity and the between studies variances were determined using Cochran (Q) and Higgins (I2) tests, respectively. A total of 55 articles met the inclusion criterion for this review and all have satisfactory quality scores. The pooled prevalence of the disease in Tanzania was 26.40%. Tanzania mainland had the highest schistosomiasis prevalence (28.89%) than Zanzibar (8.95%). Sub-group analyses based on the year of publication revealed the going up of the pooled prevalence, whereby for (2013-2018) and (2018-2023) the prevalence was 23.41% and 30.06%, respectively. The prevalence of the Schistosoma mansoni and Schistosoma hematobium were 37.91% and 8.86% respectively. Mara, Simuyu, and Mwanza were the most prevalent regions, with a pooled prevalence of 77.39%, 72.26%, and 51.19%, respectively. The pooled prevalence based on the diagnostic method was 64.11% for PCR and 56.46% for POC-CCA, which is relatively high compared to other tests. Cochrans and Higgins (I2) test has shown significant heterogeneity (p-value = 0.001 and I2 = 99.6). Factors including age, region, diagnostic method and sample size have shown significant contribution to the displayed heterogeneity. The pronounced and increasing prevalence of the disease suggests potential low coverage and possibly lack of involvement of some regions in the control of the disease. This, therefore, calls for an intensive implementation of control interventions in all endemic regions, preferably using an integrated approach that targets several stages of the disease lifecycle.

Quantifying the Capacity for Assisted Migration to Achieve Conservation and Forestry Goals Under Climate Change

ABSTRACTAimMany tree species may be threatened with declines in range and biomass, or even extinction, if they cannot disperse or adapt quickly enough to keep pace with climate change. One potential, and potentially risky, strategy to mitigate this threat is assisted migration (AM), the intentional movement of species to facilitate population range shifts to more climatically suitable locations under climate change. The ability for AM to minimise risk and maximise conservation and forestry outcomes depends on a multi‐faceted decision process for determining, what, where and how much to move. We provide an assessment on how the benefits and risks of AM could affect the decision‐making process.LocationMountainous coastal western United States.TaxonTrees.MethodsWe used a dynamic vegetation model parameterised with 23 tree species.ResultsWe found that most of the modelled species are likely to experience a substantial decline in biomass, with many potentially facing regional extinction by 2100 under the high‐emission SSP5‐85 climate‐change scenario. Though simulations show AM had little effect on the forestry goal of total biomass across all species, its effects on the conservation goal of promoting individual species' persistence were far more substantial. Among eight AM strategies (differing in the life cycle stage of movement and target destination selection criteria), the approach that conserved the highest biomass for individual species involved relocating target seedlings to areas that recently experienced fire. Although this strategy significantly reduced extinction risk for six at‐risk species compared with no action, it also slightly reduced biomass of four species, due to increasing competition. Species with relatively weak tolerance to drought, fire or high temperature were the most likely candidate groups for AM.Main ConclusionsOur simulations indicate that AM can aid conservation by reducing extinction risks for species vulnerable to climate change, but it has limited impact on forestry‐specific goals, affecting overall biomass minimally. This model framework could be applied to other forest ecosystems to evaluate the efficacy of AM globally.

Single cell transcriptional changes across the blood stages of artemisinin resistant K13580Y Plasmodium falciparum upon dihydroartemisinin exposure.

Artemisinins have been a cornerstone of malaria control, but resistance in Plasmodium falciparum, due to mutations in the Kelch13 (K13) protein, threaten these advances. Artemisinin exposure results in a dynamic transcriptional response across multiple pathways, but most work has focused on ring stages and ex vivo transcriptional analysis. We applied single cell RNAseq to two unsynchronized coisogenic parasite lines (K13C580 and K13580Y) over 6 hrs after a pulse exposure to dihydroartemisinin (DHA). Transcription was altered across all stages, with the greatest occurring at the trophozoite and ring stage in both lines. This response involved the arrest of metabolic processes, support for a dormancy phenomenon upon treatment, and the enhancement of protein trafficking and the unfolded protein response. While similar, the response was consistent across stages in K13580Y, with enhanced parasite survival to drug induced stress. Increased surface protein expression was seen in K13580Y parasites at baseline and upon drug exposure, highlighted by the increased expression of PfEMP1 and GARP, a potential therapeutic target. Antibody targeting GARP maintained anti-parasitic efficacy in K13580Y parasites. This work provides single cell insight of gene transcription across all life cycle stages revealing transcriptional changes that could initiate a dormancy state and mediate survival upon treatment.

Extended Conformational Selection in the Antigen-Antibody Interaction of the PfAMA1 Protein.

Plasmodium falciparum apical membrane antigen 1 (PfAMA1) is a surface protein found in two stages of the malaria life cycle. This is a protein involved in a reorientation movement of the parasite so that cell invasion occurs in the so-called "moving junction", relevant when the membranes of the parasite and the host are in contact. The structure of a conformational epitope of domain III of PfAMA1 in complex with the monoclonal antibody Fab F8.12.19 is experimentally known. Here, we used molecular dynamics with enhanced sampling by Hamiltonian replica exchange molecular dynamics (HREMD) to understand the effect of intermolecular interactions, conformational variability, and intrinsically disordered regions on the mechanism of antigen-antibody interaction. Clustering methods and the analysis of conformational variability were used in order to understand the influence of the presence of the partner protein in the complex. The free-state epitope accesses a broader conformational pool, including disordered conformations not seen in the bound state. The simulations suggest an extended conformational selection mechanism in which the antibody stabilizes a conformational set of the epitope existing in the free state. The stabilization of the active conformation occurs mainly through hydrogen bonds: Tyr(H33)-Asp493, His(L94)-Val510, Ser(L93)-Glu511, Tyr(H56)-Asp485, and Tyr(H35)-Asp493. The antibody has a structure with few flexible regions, and only the complementarity determining region (CDR) H3 shows greater plasticity in the presence of the epitope.

Transport-Related Emissions and Transition Strategies for Sustainability—A Case Study of the Fast Fashion Industry

The fast fashion industry significantly impacts the environment at every stage of a product’s life cycle. The transport of products to wholesalers and stores, as one of the stages of the product life cycle, is often overlooked during the analysis of their carbon footprint. Therefore, research is needed on the impact of fast fashion on the environment through its logistics and transport, especially in relation to air emissions. This article attempts to determine how extensively fast fashion companies disclose the impact of their transport operations on their overall corporate carbon footprint, determine the proportion of total emissions attributed to transport, and evaluate the effectiveness of companies’ efforts to mitigate their transport-related environmental impact. This research used a case study method on selected brands (H&M Group, Inditex, Shein), using secondary data available in non-financial reports for 2023. As a result of the analysis of their non-financial reports on transport emissions, several key findings have been presented. Companies predominantly rely on external frameworks and standards, particularly the Global Reporting Initiative. However, the level of detail of the data presented varies. The priority for the surveyed companies is to reduce emissions from significant sources, such as production processes. The data disclosed in the reports (scope 1, 2, 3) must be clearly formulated and can be used to calculate the level of their CO2 emission reduction or increase. Companies should improve their environmental reporting by including indicators of their transport-related emissions. This approach provides a comprehensive view of their environmental impact, highlighting absolute values and efficiency metrics.

The rise of checkbox AI ethics: a review

AbstractThe rapid advancement of artificial intelligence (AI) sparked the development of principles and guidelines for ethical AI by a broad set of actors. Given the high-level nature of these principles, stakeholders seek practical guidance for their implementation in the development, deployment and use of AI, fueling the growth of practical approaches for ethical AI. This paper reviews, synthesizes and assesses current practical approaches for AI in health, examining their scope and potential to aid organizations in adopting ethical standards. We performed a scoping review of existing reviews in accordance with the PRISMA extension for scoping reviews (PRISMA-ScR), systematically searching databases and the web between February and May 2023. A total of 4284 documents were identified, of which 17 were included in the final analysis. Content analysis was performed on the final sample. We identified a highly heterogeneous ecosystem of approaches and a diverse use of terminology, a higher prevalence of approaches for certain stages of the AI lifecycle, reflecting the dominance of specific stakeholder groups in their development, and several barriers to the adoption of approaches. These findings underscore the necessity of a nuanced understanding of the implementation context for these approaches and that no one-size-fits-all approach exists for ethical AI. While common terminology is needed, this should not come at the cost of pluralism in available approaches. As governments signal interest in and develop practical approaches, significant effort remains to guarantee their validity, reliability, and efficacy as tools for governance across the AI lifecycle.

Regulatory effect of N6-methyladenosine on tumor angiogenesis.

Previous studies have demonstrated that genetic alterations governing epigenetic processes frequently drive tumor development and that modifications in RNA may contribute to these alterations. In the 1970s, researchers discovered that N6-methyladenosine (m6A) is the most prevalent form of RNA modification in advanced eukaryotic messenger RNA (mRNA) and noncoding RNA (ncRNA). This modification is involved in nearly all stages of the RNA life cycle. M6A modification is regulated by enzymes known as m6A methyltransferases (writers) and demethylases (erasers). Numerous studies have indicated that m6A modification can impact cancer progression by regulating cancer-related biological functions. Tumor angiogenesis, an important and unregulated process, plays a pivotal role in tumor initiation, growth, and metastasis. The interaction between m6A and ncRNAs is widely recognized as a significant factor in proliferation and angiogenesis. Therefore, this article provides a comprehensive review of the regulatory mechanisms underlying m6A RNA modifications and ncRNAs in tumor angiogenesis, as well as the latest advancements in molecular targeted therapy. The aim of this study is to offer novel insights for clinical tumor therapy.

Impact of Artificial Intelligence in Customer Journey

The entire gamut of Customer journey is undergoing a massive transformation due to the rapid advancement of Artificial Intelligence (AI). Leveraging the power of AI , CRM & systems have refined the aspect of how businesses manage and optimize the customer journey. AI-powered systems have significant impact across various stages of the customer lifecycle by use of techniques such as machine learning to empower businesses to use systems that can analyse vast amounts of customer dataset in real-time, enabling them to gain deeper insights in customer behaviours, preferences, & sentiment. The AI-driven techniques help businesses to drive more personalized & targeted marketing campaigns, tailored recommendations, and extend efficient customer service leading ultimately to enhancing customer satisfaction and loyalty. Moreover, AI-powered systems have capabilities of offering predictive analytics which empower businesses to forecast customer behaviours and anticipate their needs. The capabilities help businesses in effective resource optimization and improve efficiency. For customer service AI-powered chatbots and virtual assistants are used to enhance engagement by providing instant responses and ability to handle resolving issues promptly.

Implementation of Site Quality Management System for a Building Construction Project

Construction industry plays an important role in the development of any country. The development of construction industry depends on the quality of construction projects. Quality is one of the critical factors in the success of construction projects. Improvement in the quality of construction projects is linked with quality management in the project life cycle. Although quality management at every stage of project life cycle is important but the quality management at the execution (construction) stage contributes significantly on final quality outcome of construction projects. This project mainly focuses the importance and factors that affects the quality management in the execution (construction) phase. The project also includes visiting of some construction companies and conducts the questionnaire survey, then analyse the difficulties (major factors) and the cost variance due to quality defect in quality management and suggests some proactive measures for the improvement of quality in the execution phase of construction projects.

Dynamics of amylopectin granule accumulation during the course of the chronic Toxoplasma infection is linked to intra-cyst bradyzoite replication.

The contribution of amylopectin granules (AG), comprised of a branched chain storage homopolymer of glucose, to the maintenance and progression of the chronic Toxoplasma gondii infection has remained undefined. Here we describe the role of AG in the physiology of encysted bradyzoites by using a custom developed imaging-based application AmyloQuant that permitted quantification of relative levels of AG within in vivo derived tissue cysts during the initiation and maturation of the chronic infection. Our findings establish that AG are dynamic entities, exhibiting considerable heterogeneity among tissue cysts at all post infection time points examined. Quantification of relative AG levels within tissue cysts exposes a previously unrecognized temporal cycle defined by distinct phases of AG accumulation and utilization over the first 6 weeks of the chronic phase. This AG cycle is temporally coordinated with overall bradyzoite mitochondrial activity implicating amylopectin in the maintenance and progression of the chronic infection. In addition, the staging of AG accumulation and it rapid utilization within encysted bradyzoites was associated with a burst of coordinated replication. As such our findings suggest that AG levels within individual bradyzoites, and across bradyzoites within tissue cysts may represent a key component in the licensing of bradyzoite replication, intimately linking stored metabolic potential to the course of the chronic infection. This extends the impact of AG beyond the previously assigned role that focused exclusively on parasite transmission. These findings force a fundamental reassessment of the chronic Toxoplasma infection, highlighting the critical need to address the temporal evolution of this crucial stage in the parasite life cycle.

Life Cycle Assessment of Industrial Wastewater Treatment Trains

AbstractAlternative technologies to granular activated carbon (GAC) are of interest to improve the sustainability and reduce the cost of munitions wastewater treatment. Research efforts have highlighted GAC alternatives, yet few reports of environmental and economic impacts associated with these technologies are available. Herein, a life cycle assessment (LCA) aids in assessment of environmental impacts associated with six munitions wastewater treatment configurations—specifically GAC, compared to five configurations that include combinations of ion exchange (IX), reverse osmosis (RO), aerobic granular reactors (AGR), UV/H2O2, and ozone technologies. The LCA compares environmental impacts generated by treating 1 m3 of munitions wastewater, impacts by life cycle stage, and effects of IX, RO, and GAC replacement frequency. Results show that IX resin pairs with AGR (for flow‐through treatment) and ozone (for IX regenerant treatment) generated 22 ± 18% less impact than GAC in nine of ten environmental impact categories during production, transportation, and disposal. Treatment trains with ozone or AGR produce 35% less environmental impact than those with UV/H2O2. Production and use stages generate more environmental impacts than transportation and disposal stages for most treatment technologies. This LCA provides insights into the sustainability of six munition wastewater treatment technologies and identifies areas where treatment sustainability can be improved.

Functional Characterization of 11 Tentative Microneme Proteins in Type I RH Strain of Toxoplasma gondii Using the CRISPR-Cas9 System.

Toxoplasma gondii, a pathogenic apicomplexan parasite, infects approximately one third of the world's population and poses a serious threat to global public health. Microneme proteins (MICs) secreted by the microneme, an apical secretory organelle of T. gondii, play important roles in the invasion, motility, and intracellular survival of T. gondii. In this study, we selected 11 genes of interest (GOIs) of T. gondii, tentative MICs predicted to be localized in micronemes, and we used the CRISPR-Cas9 system to construct epitope tagging strains and gene knockout strains to explore the localization and function of these 11 tentative MICs. Immunofluorescence assay showed that nine tentative MICs (TGME49_243930, TGME49_200270, TGME49_273320, TGME49_287040, TGME49_261710, TGME49_205680, TGME49_304490, TGME49_245485, and TGME49_224620) were localized or partially localized in the microneme, consistent with the prediction. However, TGME49_272380 and TGME49_243790 showed different localizations from the prediction, being localized in the endoplasmic reticulum and the dense granule, respectively. Further functional characterization of the 11 RHΔGOI strains revealed that deletion of these 11 GOIs had no significant effect on plaque formation, intracellular replication, egress, invasion ability, and virulence of T. gondii. Although these 11 GOIs are not essential genes for the growth and virulence of tachyzoites of type I RH strain, they may have potential roles in other developmental stages or other genotypes of T. gondii. Thus, further research should be performed to explore the possible role of the nine mics and the other two GOIs in other life cycle stages and other genotypes of T. gondii.

Life cycle perspective in design and product development

Abstract Eco-design is one of the cornerstones of the modern economy, as reflected in the policies aimed at implementing the principles of design for the environment in the European Union legislation. A life cycle perspective is a feature of eco-design. The study aimed to determine whether and to what extent the selected companies operating in manufacturing sectors consider life cycle perspectives when designing and developing the products they offer. The main research area discussed in the following article focused on the question: What kind of activities related to the idea of life cycle thinking in product policy can be identified in the analysed enterprises? Qualitative research was conducted using the individual in-depth interview method with representatives of selected industries located in Poland. Eight manufacturer groups were invited to participate in the study. Based on the recruitment process, 24 companies were chosen for the interview. Individual in-depth interviews were conducted using Microsoft Teams, following the ICC/ESOMAR Code 2016 standards. Based on the results, most companies that participated in the study considered the life cycle perspective when designing or further developing products. However, their activities varied in scope. The activities of the ten interviewed companies could be regarded as advanced. For six companies, the advanced activities targeted the product’s use phase. Two companies undertook such activities at more than one life cycle stage. The study provides evidence that the surveyed companies are beginning to think beyond operational boundaries and changing their pro-environmental orientation, albeit unimpressively. The paper provides evidence that eco-design requirements are an unquestionable driver for activities from a life cycle perspective. All surveyed manufacturers of energy-powered products are taking measures to reduce energy intensity with less activity, for example, ensuring the durability/reliability of products and finding solutions to facilitate disassembly and recycling.

Schistosoma excretory/secretory products: an untapped library of tolerogenic immunotherapeutics against food allergy.

Food allergy (FA) is considered the 'second wave' of the allergy epidemic in developed countries after asthma and allergic rhinitis with a steadily growing burden of 40%. The absence of early childhood pathogen stimulation embodied by the hygiene hypothesis is one explanation, and in particular, the eradication of parasitic helminths could be at play. Infections with parasites Schistosoma spp. have been found to have a negative correlation with allergic diseases. Schistosomes induce regulatory responses to evade immune detection and ensure their long-term survival. This is achieved via excretory/secretory (E/S) products, consisting of proteins, lipids, metabolites, nucleic acids and extracellular vesicles, representing an untapped therapeutic avenue for the treatment of FA without the unpleasant side-effects and risks associated with live infection. Schistosome-derived immunotherapeutic development is in its infancy and novel discoveries are heavily technology dependent; thus, it is essential to better understand how newly identified molecules interact with host immune systems to ensure safety and successful translation. This review will outline the identified Schistosoma-derived E/S products at all life cycle stages and discuss known mechanisms of action and their ability to suppress FA.

Impact of voluntary environmental regulation on green innovation from the perspective of enterprise life cycle: evidence from China

From the perspective of enterprise life cycle, the impact of voluntary environmental regulation on enterprise green innovation was examined in this study based on the data of manufacturing enterprises listed on China’s A-share market from 2012 to 2021. The results indicate that: (1) voluntary environmental regulation has a significant positive impact on enterprise green innovation as a whole. (2) Among all stages of enterprise life cycle, voluntary environmental regulation has a significant impact on enterprise green innovation in the growth and maturity stages, but has a relatively small impact on enterprise green innovation in the formation and decline stages. (3) The results of a segmented sample study on this basis show that voluntary environmental regulation has heterogeneity in property rights and factor density in promoting enterprise green innovation. It significantly promotes green innovation by state-owned enterprises in the maturity stage, green innovation by non-state-owned enterprises in the maturity and decline stages, and green innovation by capital and technology intensive manufacturing enterprises, but have no significant impact on other types of enterprises. These research conclusions are expected to provide decision-making reference for improving the voluntary environmental regulation system and promoting the level of enterprise green innovation.