Stages Of Development Research Articles

Ontogenetic and evolutionary trends on cephalopod digestive systems

AbstractMeropelagic octopuses hatch as planktonic paralarvae, being the subject of progressive morphological and behavioural changes ending with settlement on the seafloor as juveniles. The comparative morphological study of digestive systems can help to understand the adaptation to particular niches during this challenging plankton-benthos transition. Here, the morphometric development of the digestive system of the common octopus (Octopus vulgaris) is described through 3D microscopy techniques over the first two months of life. This morphological development is compared with those of adults and the holobenthic cuttlefish Sepia officinalis, as well as with the holopelagic squids Loligo vulgaris and Illex coindetii. Based on present and published results, we suggest four stages for the early development of O. vulgaris: (1) post embryonic, (2) early paralarval, (3) advanced paralarval and (4) early juvenile. The digestive system development was variable over time, with faster growth during the first days of planktonic life and after settlement. All the paralarvae-bearing species showed very proportionally enlarged posterior salivary glands at hatching compared both to more developed conspecifics and to the cuttlefish hatchling. This could reflect a potential common feeding mechanism through external digestion and probably a compensatory mechanism for an early digestive gland underperformance. The proportionally smallest digestive system has been found in L. vulgaris, mainly because of their remarkably smaller digestive glands. All species showed major changes in digestive organs between hatchlings and developed phases, indicating how different ecological contexts over ontogeny are reflected in their digestive structures. Graphical abstract

CNSC-11. INVESTIGATION OF GLIOBLASTOMA NEURON-TUMOR NETWORKS WITH RABIES-BASED RETROGRADE TRACING

Abstract Glioblastomas are primary brain tumors known for their whole-brain invasion and high therapeutic resistance. In previous studies, neuron-to-glioma synapses have been shown to promote glioblastoma progression. A deep characterization of this communication and of tumor-connected neurons has not been possible due to technological limitations. We adapted a rabies virus-based retrograde tracing approach to investigate and manipulate neuron-tumor networks. Interestingly, we found that glioblastoma cells rapidly integrated into neural circuits across the brain, engaging with distal neurons of diverse subtypes in very early stages of tumor development. Beyond glutamatergic neurons, we identified cholinergic neurons as drivers of glioblastoma cell invasion. We revealed patient- and tumor cell state-dependent differences in the expression of synaptogenic genes which also correlated with neuron-tumor connectivity and tumor cell invasivity. Notably, radiotherapy led to increased neuron-tumor connectivity mediated via neuronal hyperexcitability. The anti-epileptic drug perampanel alongside radiotherapy mitigated this effect and enhanced treatment efficacy by simultaneous neuronal activity inhibition. Utilizing our rabies-based approach, we genetically ablated tumor-connected neurons leading to a significantly decreased tumor progression offering a potential novel therapeutic avenue. Taken together, we developed a framework for dedicated analysis of multicellular neuron-tumor networks and propose our methodology as a versatile and adaptable approach to tackle glioblastoma.

EXTH-19. AN INTERNATIONAL ACCELERATOR PROGRAMME FOR RESEARCHERS DEVELOPING NOVEL THERAPIES FOR BRAIN TUMOURS

Abstract Developing a comprehensive translational programme for brain tumours involves navigating numerous challenges, including robustness of preclinical studies, safety, clinical trial design, understanding and addressing regulatory requirements, and funding. Recognising the difficulty of this journey, the Brain Tumour Research Novel Therapeutics Accelerator (BTR-NTA) was launched in 2023. The BTR-NTA is an accelerator programme providing independent expert guidance to de-risk drug or device development through systematic evaluation. The international, multidisciplinary BTR-NTA Committee spans expertise across the entire therapeutic development pipeline. Supported by Brain Tumour Research, the BTR-NTA programme is free for academic applicants. Researchers at any stage of therapeutic development may apply to BTR-NTA. Accepted applicants proceed as follows: 1. Applicants outline their current and prospective data and findings. The proposal and all supporting materials are covered under a confidentiality agreement that protects applicants’ intellectual property; 2. Proposals are reviewed by the BTR-NTA Committee; 3. Applicants and BTR-NTA Committee meet in-person to constructively evaluate the work; and 4. Applicants receive a comprehensive report with feedback on strengths, identification of potential risks, and guidance on next steps. The BTR-NTA Committee dedicates time for in-person meetings with each applicant, and extensive time pre- and post-meeting. Since the launch of BTR-NTA in 2023,14 international groups have applied to the programme and seven therapies have been advanced to the in-person review by the BTR-NTA Committee. Of the applicants who provided feedback on the BTR-NTA programme, all intended to adjust or refine their future work based on the Committee’s input and the average rating of the programme’s helpfulness was 9.5 out of 10. The ultimate goal of BTR-NTA is to help researchers with a potential therapy for brain tumours to navigate the development journey and reach the clinic in an efficient way. This programme will support the development of novel therapies to improve outcomes for patients with brain tumours.

Communication design logics and innovation management

AbstractEffective communication with external or internal stakeholders is crucial for innovation processes. The fourth wave of digital technologies and the fifth industrial revolution have increased the range of communication possibilities, amplifying both the number of communication channels and the availability of data. Yet in existing research that tends to treat communication as an art instead of a science, the evidence only scratches the surface of its mechanics and inner logic. To provide a unified theoretical framework, this study highlights the importance of the four pillars of communication design logic (source, situation, intentions, and impact) in the innovation processes. According to communication theories, individual differences in stakeholders' backgrounds, situations, and intentions might result in systematic variations in what and how they communicate, which influences their subsequent beliefs and reactions to innovation outcomes. By examining relevant communications in terms of their design logic, innovation researchers can address a wide range of questions across ideation, development, and implementation stages. Using novel techniques and unstructured data (e.g., text, image, video, audio mining), the current study reveals tactics for exploring such topics across communication types. The proposed framework also raises several research questions that can be pursued to enhance knowledge of how communication design influences innovation management.

PROVISION OF BANKING SERVICES IN THE CONTEXT OF THE SUSTAINABILITY OF THE BANKING SYSTEM: CURRENT TRENDS AND DEVELOPMENT DIRECTIONS

The article examines banking services in the context of the stability of the banking system: current trends and directions of development. The current stage of development of the global banking system operates under conditions of intensified competition and crisis phenomena in financial markets. One of the main factors in the successful development of the domestic banking system is the policy of constant innovations.Currently, innovation is a key factor in the stability, competitiveness and sustainable economic growth of domestic banks. The sustainable development of the national economy is accompanied by an increase in the volume of financial transactions and an increase in the importance of the domestic banking sector. The banking system, being one of the most important links of the market economy, affects the life of society in many ways. It not only provides a mechanism for interregional redistribution of monetary capital, but is also a key element of the settlement and payment mechanisms of the country's economic system. This fact determines the importance of the banking sector in the socio-economic development of the state.The current realities of the banking business contribute to the identification of the main catalysts of domestic banking innovation activity: globalization of world financial markets and markets of banking services; growing competition from the banking and non-banking sectors; the world financial crisis. The above-mentioned factors significantly affect the qualitative and quantitative composition of banking institutions and their services and lead to key changes in consumer behavior, thus leaving banks with the only opportunity to preserve and increase their competitive advantages through the introduction of innovations, the direction of which determines the strategy for the development of the banking sector on medium-term perspective. Based on the results of the research, it was established that the problems accumulated in the national banking system can be solved by applying the experience of Great Britain, and the main postulates should be adapted to the national banking system, which will contribute to the development of the domestic market of banking services and increase the stability of the national banking system.

The E3 ubiquitin ligase RNF220 maintains hindbrain Hox expression patterns through regulation of WDR5 stability

The spatial and temporal linear expression of Hox genes establishes a regional Hox code, which is crucial for the antero-posterior (A-P) patterning, segmentation, and neuronal circuit development of the hindbrain. RNF220, an E3 ubiquitin ligase, is widely involved in neural development via targeting of multiple substrates. Here, we found that the expression of Hox genes in the pons was markedly up-regulated at the late developmental stage (post-embryonic day E15.5) in Rnf220-/- and Rnf220+/- mouse embryos. Single-nucleus RNA sequencing (RNA-seq) analysis revealed different Hox de-repression profiles in different groups of neurons, including the pontine nuclei (PN). The Hox pattern was disrupted and the neural circuits were affected in the PN of Rnf220+/- mice. We showed that this phenomenon was mediated by WDR5, a key component of the TrxG complex, which can be polyubiquitinated and degraded by RNF220. Intrauterine injection of WDR5 inhibitor (WDR5-IN-4) and genetic ablation of Wdr5 in Rnf220+/- mice largely recovered the de-repressed Hox expression pattern in the hindbrain. In P19 embryonal carcinoma cells, the retinoic acid-induced Hox expression was further stimulated by Rnf220 knockdown, which can also be rescued by Wdr5 knockdown. In short, our data suggest a new role of RNF220/WDR5 in Hox pattern maintenance and pons development in mice.

Light Energy Use Efficiency in Photosystem II of Tomato Is Related to Leaf Age and Light Intensity

The fundamental key to increase photosynthetic efficiency of crop plants lies in optimizing the light energy use efficiency. In our study, we used tomato to evaluate the allocation of absorbed light energy in young and mature leaves, and to estimate if the extent of photoinhibition and photoprotection can be affected by the leaf age. A reduced efficiency of the oxygen-evolving complex, in young leaves compared to mature ones, resulted in a donor-side photoinhibition, as judged from the significantly lower Fv/Fm ratio, in young leaves. The detected increased 1O2 production in young leaves was probably due to a donor-side photoinhibition. The effective quantum yield of photosystem II (PSII) photochemistry (ΦPSII), at low light intensity (LLI, 426 μmol photons m−2 s−1), was significantly lower in young compared to mature leaves. Moreover, the non-significant increase in non-photochemical energy loss in PSII (ΦNPQ) could not counteract the decreased ΦPSII, and as a result the non-regulated energy loss in PSII (ΦNO) increased in young leaves, compared to mature ones. The significantly lower ΦPSII in young leaves can be attributed to the increased reactive oxygen species (ROS) creation that diminished the efficiency of the open PSII reaction centers (Fv’/Fm’), but without having any impact on the fraction of the open reaction centers. The reduced excess excitation energy, in mature leaves compared to young ones, at LLI, also revealed an enhanced PSII efficiency of mature leaves. However, there was almost no difference in the light energy use efficiency between young and mature leaves at the high light intensity (HLI, 1000 μmol photons m−2 s−1). The ability of mature tomato leaves to constrain photoinhibition is possible related to an enhanced photosynthetic function and a better growth rate. We concluded that the light energy use efficiency in tomato leaves is influenced by both the leaf age and the light intensity. Furthermore, the degrees of photoinhibition and photoprotection are related to the leaf developmental stage.

Production losses caused by red leather leaf in hay and milling oats

Context Red leather leaf (RLL), caused by Neospermospora avenae, is a common foliar disease of oats in south-eastern Australia but its impact on hay and grain yield and quality is unknown. Aim We aimed to determine the effect of RLL on grain yield and quality of commercial milling oat varieties, and biomass and nutrition of hay oat varieties, with a range of host plant resistance responses by comparing fungicide and disease treatments. Methods A total of eight field experiments were conducted in 2019 and 2020; four experiments each investigated the effects of RLL on hay or milling oats. In each experiment, six or seven oat varieties were sown with a disease or fungicide imposed. RLL severity was estimated visually for each experiment. Biomass yield and quality traits at early milk development stage were measured for hay oat experiments; and grain yield and quality for the milling oat experiments. Key results RLL severity (% leaf area affected: 0–44% in hay oats and 2–61% in milling oats) varied with the season, varietal resistance, and treatments. RLL caused significant losses in most experiments, with reductions in biomass of up to 3.5 t/ha (22%) in hay oats and grain yield of up to 1.1 t/ha (21%) in milling oats. Conclusions RLL can be a severe foliar disease of oats in south-eastern Australia and cause significant losses in both hay and milling oat crops. Implications Growing varieties with resistance can effectively reduce potential loss due to RLL and growers are required to adapt fungicide strategies reflecting seasonal conditions and product type (grain or hay).

Effect of Irrigation and Foliar Nutrition on Yield and Economics of Rice Fallow Blackgram

The present investigation, entitled “Effect of Irrigation and Foliar Nutrition on Yield and Economics of rice fallow blackgram” was carried out at the Agricultural College Farm, Naira, during the rabi season of 2023-24. The experiment was laid out in Strip-plot design with 3 main plots viz., M1- No Irrigation; M2-Irrigation at flower initiation and M3-Two irrigations one at flower initiation one at early pod development and 4 Sub Plots viz., S1- water spray; S2- 1%19:19:19(NPK); S3-2% Urea; S4-2% DAP; S5-4 ml Nano urea; S6-3ml Nano DAP with two replications for each treatment. The results showed that irrigation at flower initiation and early pod development stage and foliar spray of 2% DAP at flower initiation and early pod development stage recorded maximum seed yield, haulm yield and economics.

Planting time and variety effects on biomass, harvest index, and yield of irrigated soybean in mid‐Southern United States

AbstractSoybean [Glycine max (L.) Merr.] biomass and grain yield has increased over the past several decades in the mid‐southern United States. This is attributable to technological advances and improved management strategies. However, a better understanding of biomass accumulation and partitioning is needed to improve our knowledge base of varietal growth habits relative to yield, planting date, and harvest index (HI). Field experiments within a split plot arrangement in a randomized complete block design were established in 2017 and 2018 in Stoneville, MS. The study aimed to evaluate the effect of early (late‐April or mid‐May) and late (late‐May) planting on biomass, HI, and yield amongst eight soybean varieties. Soybean total biomass accumulation was collected at multiple development stages, including V4, R2, mid R5, mid R6, and R8, and partitioned into senesced leaves, pods, and seeds. Overall, the planting date had no effect on yield, HI, and biomass accumulation at any of the growth stages. Yet, the interaction between planting date and variety significantly affected biomass accumulation at the mid R5 stage. Contrarily, the variety selection significantly affected yield, HI, and biomass accumulation at all growth stages except mid R6. The total biomass accumulation at R8 was greatest for Asgrow 46X6, Asgrow 4632, Terral 4857X, Terral 48A76, and Credenz 4748, when pooled over planting dates. Averaged across two planting dates, the greatest yield was produced by Terral 48A76, Asgrow 4632, and Asgrow 46X6. Furthermore, averaged across site‐years, HI was greatest for Asgrow 4632 and Terral 48A76. Based on the results of this study, evaluating soybean HI rather than overall biomass accumulation may be more beneficial for variety selection decisions.

THE DEVELOPMENT OF PROJECT BASED LEARNING E-MODULE ABOUT STRUCTURE AND FUNCTION OF LIVING CREATURES TOPIC

The general background of this research is the limited teaching materials equipped with project-based learning assessment instruments as recommended in Kurikulum Merdeka. The purpose of this research is to produce a Project Based Learning (PjBL) based e-module on the structure and function of living creatures topic that is valid, practical and effective.The type of research used is Research and Development (R&D) with 4-D model’s consisting of the stages of defining, designing, developing and disseminating. However, this research was only untill the development stage due to time constraints. The research instruments consist of teacher questionnaires, student questionnaires, validity test questionnaires, practicality test questionnaires, and effectiveness test questionnaires. The data obtained was analyzed using a formula to analyze the results of validity, practicality and effectiveness tests. The research results show that the PjBL-based e-module developed is valid with an average 88%. The level of practicality is practical with an average 88%. The level of effectiveness is effective which is 84% ​​on average. Based on the research results, it can be concluded that the PjBL-based science learning e-module on the material Structure and Function of the Body of Living Creatures meets the valid, practical and effective categories.

Agenda of Early Life Experience and Its Association with Sensitivity to Human Presence and Familiarity in Wild-Born Orphaned Captive Cheetahs

It has been argued that domestication explains the ability of domestic animals to use human cues, but similar abilities exist in wild animals repeatedly exposed to humans. Little is known on the importance of the developmental stage of this exposure for developing such abilities. Orphancy and subsequent hand-rearing constitute a quasi-experimental situation for investigating this question. Cheetahs (Acinonyx jubatus) are interesting as they present a two-step development, spending their first two months of life in a den with occasional visits from the mother, and then accompanying their mother outdoors. Mother loss may occur at any of these stages, and the orphaned cheetahs are then taken under human care. We tested the importance of the stage of development on their long-term perception of humans by comparing the reactions of adult wild-born cheetahs, orphaned at “den-age” (Early-Orphaned, 0–2 months) or at a later stage (Late-Orphaned, 2–6 months), to motionless humans (familiar vs. unfamiliar). The results showed that Early-Orphaned, but not Late-Orphaned cheetahs, produced affiliative calls (purrs) towards humans and discriminated familiar and unfamiliar humans. Taken together, these results suggest that the timing in which an emotional bond is created with the primary caregiver (humans in the case of Early-Orphaned cheetahs and the mother in the case of Late-Orphaned cheetahs) is crucial in determining the reaction towards the species of the early caregiver throughout life.

SYMPATHIQUE: image-based tracking of symptoms and monitoring of pathogenesis to decompose quantitative disease resistance in the field

BackgroundQuantitative disease resistance (QR) is a complex, dynamic trait that is most reliably quantified in field-grown crops. Traditional disease assessments offer limited potential to disentangle the contributions of different components to overall QR at critical crop developmental stages. Yet, a better functional understanding of QR could greatly support a more targeted, knowledge-based selection for QR and improve predictions of seasonal epidemics. Image-based approaches together with advanced image processing methodologies recently emerged as valuable tools to standardize relevant disease assessments, increase measurement throughput, and describe diseases along multiple dimensions.ResultsWe present a simple, affordable, and easy-to-operate imaging set-up and imaging procedure for in-field acquisition of wheat leaf image sequences. The development of Septoria tritici blotch and leaf rusts was monitored over time via robust methods for symptom detection and segmentation, spatial alignment of images, symptom tracking, and leaf- and symptom characterization. The average accuracy of the spatial alignment of images in a time series was approximately 5 pixels (~ 0.15 mm). Leaf-level symptom counts as well as individual symptom property measurements revealed stable patterns over time that were generally in excellent agreement with visual impressions. This provided strong evidence for the robustness of the methodology to variability typically inherent in field data. Contrasting patterns in the number of lesions resulting from separate infection events and lesion expansion dynamics were observed across wheat genotypes. The number of separate infection events and average lesion size contributed to different degrees to overall disease intensity, possibly indicating distinct and complementary mechanisms of QR.ConclusionsThe proposed methodology enables rapid, non-destructive, and reproducible measurement of several key epidemiological parameters under field conditions. Such data can support decomposition and functional understanding of QR as well as the parameterization, fine-tuning, and validation of epidemiological models. Details of pathogenesis can translate into specific symptom phenotypes resolvable using time series of high-resolution RGB images, which may improve biological understanding of plant-pathogen interactions as well as interactions in disease complexes.

Study on the Swimming Behavior of Black Carp (Mylopharyngodon piceus) and Silver Carp (Hypophthalmichthys molitrix) in Early Developmental Stage

Fish need to make different responses to different water flow conditions, as a variety of fish utilize or overcome the water flow while fish are in the water. This study aims to analyze the response of two types of carp (black carp and silver carp) to water flow in different body lengths (2.0–10.0 cm) in early developmental stages in an open channel. By analyzing the water velocity and fish swimming behavior, swimming against the water flow was the most frequent swimming behavior, and the frequencies of this behavior increased with the increased body length of fish. The results demonstrated that the detectable water velocity of two carp species of different body lengths remained in a certain range (0.020–0.060 m/s). Black carp and silver carp can swim against the water flow of 0.295–0.790 m/s and 0.245–0.825 m/s, respectively. The results of this study can provide invaluable data for engineers and biologists to protect fish in the early developmental stage and restore the ecological habitats in the regulated river systems.

Masting ontogeny: the largest masting benefits accrue to the largest trees

Abstract Background and Aims Both plants and animals display considerable variation in their phe- notypic traits as they grow. This variation helps organisms to adapt to specific challenges at different stages of development. Masting, the variable and synchronized seed production across years by a population of plants, is a common reproductive strategy in perennial plants that can enhance reproductive efficiency through increasing pollination efficiency and decreasing seed predation. Masting represents a population-level phenomenon generated from individual plant behaviors. While the developmental trajectory of individual plants influences their masting be- havior, the translation of such changes into benefits derived from masting remains unexplored. Methods and Key Results We used 43 years of seed production monitoring in European beech (Fagus sylvatica) to address that gap. The largest improvements in reproductive efficiency from masting happen in the largest trees. Masting leads to a 48-fold reduction in seed predation in large, compared to 28-fold in small trees. Masting yields an 6-fold increase in pollination efficiency in large, compared to 2.5-fold in small trees. Paradoxically, although the largest trees show the biggest reproductive efficiency benefits from masting, large trees mast less strongly than small trees. Conclusions That apparently suboptimal allocation of effort across years by large plants may be a consequence of anatomical constraints or bet-hedging. Ontogenetic shifts in individual mast- ing behavior and associated variable benefits have implications for the reproductive potential of plant populations as their age distribution changes, with applications in plant conservation and management.

From the genome's perspective: Bearing somatic retrotransposition to leverage the regulatory potential of L1 RNAs

AbstractTransposable elements (TEs) are mobile genomic elements constituting a big fraction of eukaryotic genomes. They ignite an evolutionary arms race with host genomes, which in turn evolve strategies to restrict their activity. Despite being tightly repressed, TEs display precisely regulated expression patterns during specific stages of mammalian development, suggesting potential benefits for the host. Among TEs, the long interspersed nuclear element (LINE‐1 or L1) has been found to be active in neurons. This activity prompted extensive research into its possible role in cognition. So far, no specific cause‐effect relationship between L1 retrotransposition and brain functions has been conclusively identified. Nevertheless, accumulating evidence suggests that interactions between L1 RNAs and RNA/DNA binding proteins encode specific messages that cells utilize to activate or repress entire transcriptional programs. We summarize recent findings highlighting the activity of L1 RNAs at the non‐coding level during early embryonic and brain development. We propose a hypothesis suggesting a mutualistic relationship between L1 mRNAs and the host cell. In this scenario, cells tolerate a certain rate of retrotransposition to leverage the regulatory effects of L1s as non‐coding RNAs on potentiating their mitotic potential. In turn, L1s benefit from the cell's proliferative state to increase their chance to mobilize.

The Paths of Ecological Civilization Construction under the Concept of Sustainable Environmental Education

Ecological civilization is a new stage of human civilization development after industrial civilization, and it is an inevitable choice to cope with the global ecological crisis and achieve harmonious coexistence between humans and nature. With the deepening of China's ecological civilization construction, sustainable development education has gradually become an important means of cultivating public ecological awareness. This article first elaborates on the connotation of ecological civilization and its relationship with education, analyzes the current situation of ecological education in schools and society, and points out the existing problems. We must adhere to the integration of ecological civilization and sustainable development education, the integration of ecological civilization and educational governance, and develop education with the people as the center, building a path for the development of ecological civilization.

Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty

The pig is an important model for studying human diseases and is also a significant livestock species, yet its testicular development remains underexplored. Here, we employ single-cell RNA sequencing to characterize the transcriptomic landscapes across multiple developmental stages of Bama pig testes from fetal stage through infancy, puberty to adulthood, and made comparisons with those of humans and mice. We reveal an exceptionally early onset of porcine meiosis shortly after birth, and identify a distinct subtype of porcine spermatogonia resembling transcriptome state 0 spermatogonial stem cells identified in humans, which were previously thought to be primate specific. We also discover the persistent presence of proliferating progenitors for myoid cells in postnatal testes. The regulatory roles of Leydig cell steroidogenesis and estrogen synthesis in supporting cell lineages are also explored, including the potential impact of estrogen on Sertoli cell maturation and spermatogenesis. Overall, this study offers valuable insights into porcine testicular development, paving the way for future research in reproductive biology, advancements in agricultural breeding, and potential applications in translational medicine.

Characterization and expression analysis of the B3 gene family during seed development in Akebia trifoliata

BackgroundB3 genes encode transcription factors that play key roles in plant growth and development. However, the specific B3 genes involved in the seed development of Akebia trifoliata remain unexplored.ResultsA total of 72 AktB3 genes were identified and classified into five subfamilies (ARF, LAV, RAV, HSI, and REM) based on phylogenetic analysis. These 72 AktB3 genes were unevenly distributed across 16 chromosomes. Collinear analysis indicated that segmental duplication has played a significant role in the evolution of AktB3 genes, and underwent purification selection. Expression profiling across seed development stages revealed that seven AktB3 genes, particularly from the LAV subfamily (AktABI3, AktFUS3, AktLEC2), were up-regulated at 70 days after flowering (DAF). Notably, the expression of oleosin exhibited a strong positive correlation with LAV subfamily genes, highlighting their potential roles as hub genes in lipid metabolism and seed development. Yeast two-hybrid (Y2H) and yeast one-hybrid (Y1H) experiments confirmed that AktFUS3-1, AktFUS3-2, and AktLEC2 form protein complexes and individually bind to the AktOLE1 promoter, thereby regulating downstream gene expression. These results provide direct evidence of the cooperative role these transcription factors play in controlling lipid metabolism, particularly related to oleosin proteins. Additionally, miRNA sequencing across three seed developmental stages identified 591 miRNAs and 1,673 target gene pairs. A total of 23 AktB3 genes were predicted to be targets of 20 miRNAs, with 11 miRNAs specifically targeting the ARF subfamily genes. Particularly, miR160-x, miR160-z, and miR167-z were predicted to target ARF subfamily genes, potentially influencing seed development. Moreover, the miRNA-B3 regulatory modules, especially involving ARF genes and miR160/167, require further study to clarify their roles in seed development.ConclusionsThese findings contribute valuable resources for future functional studies of the molecular regulatory networks governing seed development in A. trifoliata.

Challenges and Strategies for the Sustainable Architectural Education in the Context of Post-Urbanization and Climate Change

The transformation of urbanization patterns due to the pandemic and climate change have significantly reshaped construction market demands, presenting substantial challenges to current architectural education (AE) models. In order to address these challenges and propose effective reform strategies, this study utilizes questionnaires and expert interviews to gather perspectives from architecture students and professionals in a Chinese university. The findings highlight three main issues in AE: (1) misalignment between talent development stages and educational goals; (2) slow updates to curriculum content, leading to lagging behind the needs of times; and (3) inadequate integration of curriculum design with practical application, coupled with excessively lengthy academic programs. To address these challenges, the study proposes the strategies: (1) adopting multi-objective and multi-track approaches for educational goals; (2) reducing length of programs and implementing flexible academic structures; and (3) developing curricula that integrate knowledge with practice. This research offers valuable insights for reforming architectural education within the framework of sustainable development, emphasizing the need for educational goals, curriculum, and cultivation system to evolve to meet the demands of modern practice and development.