The study examined the influence of job burnout on employee performance among academic staff of tertiary institutions in Ekiti State. Specifically, the study investigated the influence of emotional exhaustion; examined the influence of depersonalization and determined the influence of personal accomplishment on employee performance among academic staff of tertiary institutions in Ekiti State. A descriptive survey research design was adopted for the study. The population of the study comprised academic staff of tertiary institutions in Ekiti State. The sample size was 344 respondents and was determined through Yamani sampling model. Primary data used for the study were gathered through the administration of structured questionnaire. Data gathered were analyzed using multiple regression. The result showed that emotional exhaustion has significant effect on employee productivity (t=3.454, t= 2.776 and t=-2.910, p<0.05); It further showed that depersonalization has significant effect on employee performance (t=1.397, t=3.314 and t=2.697, p<0.05); Furthermore, it showed that personal accomplishment has significant effect on employee performance (t=4.054, t=4.452, and t= 5.086, p<0.05). The study concluded that all the constructs (emotional exhaustion, depersonalization and personal accomplishment) of the explanatory variables were significantly related to employee performance among academic staff of tertiary institutions in Ekiti State, Nigeria. Keyword: Depersonalization, Emotional Exhaustion, Employee, Performance, Job Burnout, Personal Accomplishment. Proceedings Citation Format Faleye, O.C. & Adelugba, I.A. (2024): Influence of Job Burnout on Employee Performance Among Academic Staff Of Tertiary Institutions In Ekiti State, Nigeria. Proceedings of the 38th iSTEAMS Multidisciplinary Bespoke Conference. 15th–19th July, 2024. University of Ghana, Accra, Ghana. Pp 143-162. dx.doi.org/10.22624/AIMS/ACCRABESPOKE2024P15