SUMMARYStomatal responses to different concentrations and durations of azide treatment were investigated by microscopic examination of epidermal strips of Stachytarpheta indica fixed in absolute ethanol. Both opening on illumination and closure on darkening of turgid leaves were suppressed by azide, which also arrested starch hydrolysis and accumulation of potassium in the guard cells in light, and starch synthesis and disappearance of stomatal potassium in the dark. The magnitude of the azide effect is a function of concentration and duration of the treatment. Carbon dioxide‐free air was effective in partly reversing the azide effect. It is concluded that azide suppresses stomatal movement partly by disturbing the carbon dioxide balance in the leaf and partly by hindering starch metabolism and transfer of potassium ions across the guard cell membranes.