Stable Oxygen Isotope Research Articles

Estimation of Surface and Groundwater Interaction by Stable Isotopic Techniques – A Case Study of Chengalpattu District, OMR Region

Isotopes are atoms of an element having the same atomic number but different mass numbers. Isotopes in hydrology and water resources are used for identifying its occurrence, movement, residence times, recharge, and discharge process. Stable isotopes of hydrogen(δ2H) and oxygen(δ2O) are used for identifying the surface and groundwater interactions as they constitute hydrogen and oxygen. In this study oxygen and hydrogen stable isotopes are used to identify surface and groundwater interaction in Old Mahabalipuram Road (OMR) regions of Chengalpattu district. The precipitation, lake, surface, and groundwater were collected during pre-monsoon, monsoon, and post-monsoon seasons. The collected sample is analyzed for stable isotopic compositions of oxygen and hydrogen seasonal-wise. The measured stable isotopic compositions during pre-monsoon season of stable oxygen are -4.29 to -2.00 and stable hydrogen are -29.39 to -24.67. The isotopic compositions during monsoon season range from -4.72 to -4.00 and for hydrogen ranges from -29.39 to -23.50. During monsoon season the depletion of isotopic composition is seen and the enrichment of isotopic composition is observed during pre-monsoon season. The variation in stable isotopic composition of oxygen and hydrogen are observed. A Groundwater Water Meteoric Water Line (GMWL) is developed for the study area, and it is compared with a Local Meteoric Water Line (LMWL) for better interpretation of the results. A slight deviation is observed from that of GMWL to LMWL mostly due to isotopic depletion and evaporation effects. From the analysis, a good correlation exists between precipitation and surface water in the study area indicating about recharge mechanism existing in the study area. The groundwater recharge is observed during monsoon seasons and discharge is more towards the pre-monsoon seasons.

Facies architecture, pedogenesis and palynology of the Olenekian dryland setting – A sedimentary record of Early Triassic climate oscillations in the SE Germanic Basin

The Early Triassic period was characterised by significant climatic perturbations driven by generally high global temperatures. Several significant shifts in the stable carbon and oxygen isotopes were recognised in the marine settings in the Olenekian age deposits that were linked to short-lasting but relatively high-amplitude climatic events. The record of these events in the continental strata is far less understood due to the stratigraphic incompleteness of such deposits. However, in the Holy Cross Mountains (Poland), the Olenekian continental deposits are well preserved, and sedimentation was relatively continuous, making them an ideal candidate for studying the effects of climate changes and their preservation in continental settings. As such, sedimentological analysis of five wells and one outcrop (over 530 m logged in total), integrated with new and legacy palynological data, allowed the reconstruction and dating of major steps in the palaeoenvironmental evolution of the SE part of the Central European (Germanic Basin), and its comparison with the marine-based climatic models for the Early Triassic. The lower and middle Olenekian stage is represented by fine-grained deposits of dry floodplain interbedded with sandstones deposited within fluvial distributary channels and terminal splays. The palaeosols are mainly represented by aridisols (calcisols), indicating a relatively dry, though not extreme, climate as some water was necessary to support relict plant cover. Towards the mid-Spathian times, the climate became more humid - lacustrine deposits appeared, and palaeosols displayed features of an elevated water table (gleyed vertisols and inceptisols). Frequent storms and floods also affected the lacustrine sedimentation, reflecting volatile climatic conditions. In the late Spathian time, continental sedimentation continued, and the dominance of vertisols and more stacked fluvial channels in the sequence is linked with an increased seasonality, with more pronounced humid seasons. The continental setting lasted until the latest Spathian time, when a marine transgression occurred, marking a new phase in the basin development.

Understanding the Source and Evolution of Precipitation Stable Isotope Composition across O‘ahu, Hawai‘i

Abstract Pacific Islands present unique challenges for water resource management due to their environmental vulnerability, dynamic climates, and heavy reliance on groundwater. Quantifying connections between meteoric, ground, and surface waters is critical for effective water resource management. Analyses of the stable isotopes of oxygen and hydrogen in the hydrosphere can help illuminate such connections. This study investigates the stable isotope composition of rainfall on O‘ahu in the Hawaiian Islands, with a particular focus on how altitude impacts stable isotope composition. Rainfall was sampled at 20 locations from March 2018 to August 2021. The new precipitation stable isotope data were integrated with previously published data to create the most spatially and topographically diverse precipitation collector network on O‘ahu to date. Results show that δ18O and δ2H values in precipitation displayed distinct isotopic signatures influenced by geographical location, season, and precipitation source. Altitude and isotopic compositions were strongly correlated along certain elevation transects, but these relationships could not be extrapolated to larger regions due to microclimate influences. Altitude and deuterium excess were strongly correlated across the study region, suggesting that deuterium excess may be a reliable proxy for precipitation elevation in local water tracer studies. Analysis of spring, rainfall, and fog stable isotope composition from Mount Ka‘ala suggests that fog may contribute up to 45% of total groundwater recharge at the summit. These findings highlight the strong influence of microclimates on the stable isotope composition of rainfall, underscore the need for further investigation into fog’s role in the water budget, and demonstrate the importance of stable isotope analysis for comprehending hydrologic dynamics in environmentally sensitive regions.

Hydrochemical processes and inorganic nitrogen sources of shallow groundwater in the Sanjiang Plain, northeast China.

This study investigates the chemical characteristics, formation, and sources of inorganic nitrogen (IN) of shallow groundwater across the Sanjiang Plain, aiming to enhance drinking water safety management and pollution control. A total of 167 groundwater and 27 surface water samples were collected for constituents and isotopes (H2 and O18). The hydrogeochemical characteristics showed that the major type is HCO3- Ca·Mg, with low total dissolved solids and a neutral to weak alkaline nature. Rock weathering processes govern the hydrochemical composition of groundwater. Hydrogen and oxygen stable isotopes analyses revealed that precipitation serves as the main water source. In alluvial areas, oxidative conditions lead to the enrichment of NO3-N concentrations, with sewage, manure, and fertilizers being the primary IN sources. In lacustrine areas, intensive rice cultivation results in reductive conditions and strong denitrification processes, causing the loss of NO3-N and leaving NH4-N as the dominant IN form. Organic matter mineralization is likely a more significant contributor to NH4-N concentrations than ammonium fertilizers. These findings provide valuable information for further research on natural sources and groundwater pollution in areas with similar hydrogeological conditions. PRACTITIONER POINTS: Rock weathering processes govern the hydrochemical composition of groundwater, and precipitation serves as the main water source. In alluvial areas, oxidative conditions lead to the enrichment of NO3-N. In lacustrine areas, intensive rice cultivation results in reductive conditions and strong denitrification processes. Organic matter mineralization is likely a more significant contributor to NH4-N concentrations than ammonium fertilizers. These findings provide references for water management and information for further research on natural sources and groundwater pollution in areas with similar hydrogeological conditions.

Footwall refrigeration versus footwall hydration: Reassessing steep thermal gradients below detachment faults with in situ SIMS oxygen isotope analysis

The extensional detachment systems of metamorphic core complexes (MCC) have the potential to facilitate significant fluid movement between Earth's surface and the warm, ductile middle crust. One long-standing and influential detachment fault-fluid interaction model called the “footwall refrigeration hypothesis” (Morrison and Anderson, 1998) postulates that cool meteoric fluids circulating downward along a detachment can facilitate large-scale heat loss at depth before rocks are exhumed by faulting. Stable isotopes of oxygen and hydrogen have long been recognized as potentially useful tracers of surface-to-depth fluid flow in core complexes; however, typical sampling methods and the competing effects between temperature and fluid composition have made oxygen isotope signatures of meteoric fluid infiltration difficult to detect and/or interpret in the rock record.Here, we use in situ δ18O measurements by secondary ion mass spectrometry (SIMS) to address the rock record of meteoric fluid interactions and temperature variation within the Whipple Mountains MCC footwall beneath the Whipple detachment fault (WDF). The micro-scale sampling of SIMS (10 µm diameter x 1–2 µm deep pits) allows investigation of isotope variability as a function of mineral geochemistry and microstructure and enables more accurate interpretation of the causes of isotope variability. In the Whipple footwall mylonites, quartz and epidote show grain-to-grain oxygen-isotope variability within samples and as a function of distance from the main detachment. Approaching the WDF, both quartz and epidote show increasing spread toward low δ18O values. The lowest SIMS-measured δ18O epidote values require exchange with a low δ18O, meteoric fluid at high temperature. However, SIMS data also reveal that meteoric fluid-rock interactions were spatially heterogeneous at the scale of individual grains, resulting in local preservation of metamorphic quartz-epidote oxygen isotope equilibrium unaffected by infiltrating meteoric fluid. Within preserved metamorphic domains at all investigated structural levels of the WDF footwall, quartz and epidote δ18O values are tightly clustered and yield similar fractionations (∆18Oqz-ep). For samples from the longest footwall traverse, these ∆18Oqz-ep values are 4.0 ± 0.4‰, consistent with δ18O equilibrium at 521+43/-37 °C. We conclude that there is no evidence of a large paleo-thermal gradient associated with the Whipple Detachment Fault (i.e., no evidence for footwall refrigeration).

Depositional control on composition, texture and diagenesis of modern carbonate sediments: A comparative study of tidal channels and marshes, Abu Dhabi, United Arab Emirates

Petrography, geochemistry, radiocarbon dating, and porewater chemistry of modern carbonate sediments in the mangrove marshes and tidal channels along the Abu Dhabi coast, United Arab Emirates, were conducted to compare sediment composition, texture and diagenesis between these two adjacent but distinct depositional environments. Tidal currents and extensive micritization of the allochems in the tidal channels have led to the enrichment of skeletal fragments (average 25 %) and peloids (average 70 %). Ooids, however, are relatively scarce (average 1 %), which is attributed to strong tidal currents flushing them out of channels and depositing them on shoals and deltas. The severe environmental conditions in the tidal channels forced the microorganisms to bore into allochems, promoting micritization via carbonate dissolution and reprecipitation of spheroidal microbial micrite. Spheroidal micrite with the same mineral composition as the host skeletal fragments fills the microbial borings, indicating that micritization does not involve mineralogical alteration. Radiocarbon dating suggests that microbial boring is an important source of micrite, which is transferred into the marshes from channels by tidal currents. The oxidizing environment in marshes, due to the presence of mangrove pneumatophores and crab burrows, reduces the likelihood of anaerobic respiration. In contrast, microbial sulfate reduction and carbonate dissolution induced by microbial boring in tidal channels caused an increase in porewater alkalinity and dissolved inorganic carbon (DIC) concentration, resulting in more abundant aragonite and high-Mg calcite cements. Stable carbon (+2.3 ‰ to +4.6 ‰) and oxygen (+0.8 ‰ to +1.5 ‰) isotopes of the allochems and micrite corroborate derivation of DIC from seawater. The formation of rare scattered rhombic dolomite as cement only in tidal channels is attributed to microbial metabolic processes. This study provides important insights into the characteristics and controlling factors of diagenesis in modern carbonate sediments, which can have wide implications for understanding the early diagenesis of ancient limestones.

Deciphering the transfer of hydroclimate signals to tree-ring δ18O using a proxy system model in East Asia's Meiyu region

The stable oxygen isotope ratios of whole tree-ring α-cellulose (δ18OWR) have been interpreted as an indicator of early summer hydroclimate in the Meiyu region of East Asia. However, the underlying physical mechanism often remains unclear. Here we provide a mechanistic understanding through intra-annual tree-ring oxygen isotope analysis and process-based δ18OWR modelling over the period 1979–2006. The selected tree species for analysis is Pinus taiwanensis, whose δ18OWR exhibit the strongest linear relationship with relative humidity (RH) in June. The results indicated that the June RH signal is predominantly contained in tree-ring earlywood rather than latewood. The strong response of δ18OWR to June RH is not due to the legacy effect. Using the proxy system model (PSM) of δ18OWR, we obtained a modeled δ18OWR time series that is significantly positively correlated with the measured δ18OWR time series. The modeled and measured δ18OWR series show similar relationships with monthly RH. Sensitivity experiments with PSM revealed that the June RH signal is originated from the oxygen isotopes of source water and leaf water. Rapid cellulose formation in June plays a role in enhancing the June RH signal. Our study demonstrates how δ18OWR record early summer hydroclimate signals from a process perspective, and that the PSM is effective in modelling the interannual δ18OWR variability in the Meiyu region.

Isotope-based source assessment of water flowing from storm sewer systems to a receiving river during dry weather periods

Urban stormwater management systems, particularly storm sewers, are critical for managing runoff in urban areas. These systems are designed to function during wet weather events; however, field-based observations of these systems suggest that they may also be active flow pathways in dry weather conditions, ultimately contributing to streamflow. Unlike dry weather flow in wastewater systems, storm sewer dry weather flow has not been thoroughly explored. This research used stable isotopes of oxygen and hydrogen in water to examine the sources of dry weather flow from storm sewers in a highly urban catchment. A stable isotope mixing model was applied at the outfalls of two stormwater catchments and the receiving Black Creek, located in Toronto, Canada. Findings suggest that during dry periods, storm sewers receive non-stormwater inputs from tap water, wastewater, and groundwater, along with some precipitation, and that these sources may constitute up to 19 % of Black Creek's flow at the watershed scale. Seasonal patterns in flow and water sources were observed for the Black Creek and outfalls. At one outfall, dry weather flow was predominantly from the water distribution system (i.e., tap water and/or wastewater) throughout spring, summer, and fall. In contrast, at the second outfall, groundwater dominated in spring and summer, and groundwater and water distribution were equally proportioned in fall. Black Creek baseflow comprises a dynamic mix of water sources that at times are similar to the sources observed at the stormwater outfalls. Considering these findings, future work should incorporate strategic sampling of additional outfalls, and multiple years of data collection to explore inter-annual variability in these processes and focus on replicating a similar study in other urban watersheds with different climates and/or water infrastructure design. The study findings highlight that our understanding of dry weather flow from storm sewers is relatively limited, emphasizing the need for further exploration of this phenomenon to inform urban hydrological modelling, water quality studies, and urban water management.

A review of benthic foraminiferal oxygen and carbon isotopes

Stable isotopes in the calcium carbonate of benthic foraminifera provide important paleoenvironmental information about seawater/sedimentary porewaters that these foraminifera live in. Oxygen isotopes provide essential insights about variations in deep water temperatures and sea-level/ice volume changes, while carbon isotopes provide information about sea-water carbon/nutrient cycling. In this review we look into detail at the direct and indirect mechanisms that contribute to stable oxygen and carbon isotope signals in Rotaliid benthic foraminifera. This includes effects from ontogenetic- and calcification mechanisms, and the impact of methane seeps and post-depositional diagenesis. We conclude our review with an overview of current challenges and provide recommendations for future research endeavors aimed at outstanding knowledge gaps in understanding how these biomineralizers control their stable isotopes.

The vertical calcification mode of planktonic foraminifera in the Western Pacific Warm Pool

A thorough knowledge of the ecology of planktonic foraminifera throughout the water column is necessary for a reliable reconstruction of the paleooceanographic environment of the upper-middle water column. However, little is known about the planktonic foraminifera in the Western Pacific Warm Pool (WPWP), especially for the deep dwelling species. Here we present the stable oxygen isotope (δ18O) data of eight species collected from vertically stratified plankton tows across 0–500 m water depth from 5 sites in the WPWP during autumn, the vertical mode of foraminiferal calcification is explored. Vertical plankton tows provide more detailed information than other materials, it indicates a wider depth range of foraminiferal calcification than the other sample types. All the species exhibit a complex vertical calcification process that the majority of specimens precipitate their tests out of the depth at which they were obtained. It could be connected to the intricate hydrological conditions in the WPWP and/or the foraminiferal ontogenetic trajectory. Calcification depth range of the investigated species is substantially smaller than their living depth, and most of them prefer to calcify inside the abundant layers. Additionally, we found no clear correlation between foraminiferal test size and shell δ18O for any of the species that were studied at any of the locations.

Sclerochronology of the large scallops Gigantopecten latissimus and Pecten jacobaeus in a Pliocene warmer Mediterranean Sea

Here we apply a sclerochronological approach to reconstruct the life-history of two stenohaline bivalves of the family Pectinidae, Gigantopecten latissimus and Pecten jacobaeus from the Pliocene of Italy. The specimens come from the locality of Torrita di Siena (Siena-Radicofani Basin, Tuscany), dated to the late Zanclean-Piacenzian with nannoplankton biostratigraphy and Sr-isotope stratigraphy. After measuring the width of micro-growth increments and verifying that the shells were not diagenetically altered, we sampled them at high resolution for carbon and oxygen stable isotopes. δ13C and δ18O shell values allowed to distinguish between specimens that lived above or below the thermocline. Those influenced by surface waters indicate temperatures (for δ18Oseawater = 1.5‰) with winter minima of 16–18 °C and summer values up to 28–29 °C, close to present temperature conditions in the tropical west-African climate belt. In line with this, we found that the two species had similar seasonal growth patterns, with faster growth during colder months and summer slowdown, a typical adaptation of bivalves of tropical affinity suffering from summer temperature extremes. Despite this similar adaptation, G. latissimus, with large and heavy shells (length up to 30 cm) became extinct around 3.0 Ma, while the smaller P. jacobaeus survived Plio-Pleistocene cooling. Different growth rates between the two species and, therefore, different metabolic costs, might explain such differential response, together with differences in reproduction strategies. Habitat loss and fragmentation, due to the decrease of shelf margins and organogenic substrates caused by cooling and sea level fall, are abiotic factors that could have also contributed to the extinction of G. latissimus. P. jacobaeus adaptation to live across a larger bathymetric range, which indicates the ability to thrive in a wider range of temperatures, most likely played a role in its survival. Further studies, including more specimens across multiple localities, will help verifying these hypotheses.

Tracing sulfate sources in a tropical agricultural catchment with a stable isotope Bayesian mixing model

Sulfate (SO42−) is an essential anion in drinking water and a vital macronutrient for plant growth. However, elevated sulfate levels can impact ecosystem or human health and could be an important indicator of acid rock drainage or pollution. Therefore, monitoring SO42− sources and transport is important for water quality assessments. This study focused on exploring the sources and transformations of SO42− as well as estimating the proportional contribution of the potential SO42− pollutant sources to groundwater and surface water in a tropical river basin, the Densu River Basin. The study used major ions combined with stable sulfur and oxygen isotope compositions and a Bayesian isotope mixing model, MixSIAR. The major ion characteristics indicate that SO42− concentrations remain stable throughout the rainy and dry seasons but originate from diverse sources. The multi-isotope model (δ34SSO4, δ18OSO4) identified four potential SO42− sources: detergent, precipitation, sewage, and sulfate fertilizer. However, the δ34SSO4 and δ18OSO4 values of the fertilizer source signatures overlapped with those of precipitation and sewage. Nevertheless, the contributions from each source were disentangled using the MixSIAR model, which revealed sewage as the most dominant SO42− pollutant in the Densu Basin, accounting for ~47 % of sulfate in groundwater and ~ 56 % of sulfate in surface water. Sulfate fertilizer (~33 %) was the second most important source after sewage for groundwater, while detergent (~23 %) was the second most important source for surface water. The redox processes of bacterial sulfate reduction and sulfide oxidation were determined to have a minimal impact on the sulfur isotope fractionation within the basin. This study highlights the benefits of combining major ions, sulfur isotopes and the MixSIAR model for identifying sources of sulfate. This approach accounts for uncertainties in source contributions which allows for more robust and reliable apportionment of sulfate sources. The study emphasizes the need for effective waste management and pollution control measures to protect water quality and provides vital guidelines on how to partition sulfate sources on a large catchment scale and evidence for making pollution management decisions on water resources.

Growth characterization of mesophotic rhodoliths in the northern Gulf of Mexico using radiocarbon dating

Boxwork rhodoliths are characterized by a multitaxonomic composition and excessive void spaces, and they are distributed from intertidal to mesophotic depths. Growth rates and patterns in rhodoliths from shallower depths and simpler morphologies have been assessed using incremental and geochemical analyses, but rhodoliths from mesophotic depths have been harder to access and require more studies. Boxwork rhodoliths have proved too structurally complex for age and growth rate assessment techniques such as stable oxygen isotopes profiling or elemental mapping. We therefore utilized sequential radiocarbon analysis for growth characterization of mesophotic boxwork rhodoliths. Rhodoliths were collected from the Flower Garden Banks National Marine Sanctuary in the northern Gulf of Mexico from one location at 48–72 m and another at 103 m. Specimens were extensively sampled for radiocarbon concentrations from predicted nucleation points and along growth axes. From depths of 48–72 m, nucleation ages ranged from 795 ± 20 to 3270 ± 25 14C yr B·P. From depths of 103 m, nucleation ages ranged from 8960 ± 110 to 13,050 ± 150 14C yr B.P., dating back to the end of the Pleistocene in some cases. Several rhodoliths contained evidence of growth hiatuses, with deeper samples displaying age reversals that complicated the apparent growth history. We define age reversals to be non-sequential ages within a sampling sequence, typically a singular age before the return to normal age sequence. The research presented here includes the oldest known living rhodoliths, with the oldest rhodoliths grown before and after Holocene sea-level stabilization. These results have implications for the use of rhodoliths as paleoenvironmental indicators, and provide better understanding of the range and volume of carbonate production in these habitats.

Soil water dynamics and plant water uptake: Primeval mature vs. debris flow-developed half-mature subalpine fir stands in the eastern Tibetan Plateau

Natural disaster can disrupt soil structure and replace established vegetation with younger plants, altering the local hydrological processes. We used hydrogen and oxygen stable isotopes to examine soil water dynamics and plant water uptake patterns in two adjacent fir stands in the eastern Qinghai-Tibet Plateau: a primeval mature stand (MF, finer- textured soil) and a debris flow-developed half-mature stand (HMF, coarser-textured soil). Our results showed that the isotopic composition and soil gravimetric water content (SWC) in deep soil water in MF exhibited a more pronounced hysteresis pattern in response to precipitation compared to HMF, indicating lower turnover rate of soil water in MF. This was also confirmed by a smaller contribution of preferential flow to deep soil water in MF compared to HMF. The higher water storage (higher SWC values) and lower turnover rate of soil water suggest a higher soil water buffer capacity in MF. Additionally, both stands showed no significant difference in plant water sources, but plants in MF used more winter precipitation due to the lower soil water turnover rate. These differences suggest MF may be more vulnerable to water disasters, while HMF may be more susceptible to seasonal droughts under climate change. Our insights enhance understanding of hydrological processes linked to changing surface conditions and offer valuable information for managing forest water resources in mountainous regions.

Transient micropaleontological turnover across a late Eocene (Priabonian) carbon and oxygen isotope shift on Blake Nose (NW Atlantic)

Abstract. The Gulf Stream, a western boundary current transporting warm water into the North Atlantic, plays a key role in climate regulation and oceanographic stability at a regional and global scale as part of the Atlantic Meridional Overturning Circulation (AMOC). Evidence suggests that an ancestral Gulf Stream has existed since the Mesozoic, and it has altered its course repeatedly over Cenozoic times. In this study, we focus on the upper Eocene (Priabonian, ca. 36 Ma) from Ocean Drilling Program Site 1053 on Blake Nose (subtropical North Atlantic). Bulk carbon and oxygen stable isotopes, as well as benthic foraminiferal and calcareous nannofossil assemblages, provide an integrated assessment of the palaeoceanographic changes impacting the area through the water column to the seafloor. Micropaleontological assemblages suggest changes in surface ocean stratification and nutrient supply to the seafloor coeval with a paired negative carbon and oxygen isotope excursion and the return to background conditions higher up in the study section. These transitory changes are compatible with the longitudinal displacement of the proto-Gulf Stream and its related eddies. Our results build on previous work and support the hypothesis that links palaeoceanographic changes in the Blake Nose area with shifts in the proto-Gulf Stream during the middle and late Eocene.

Responses of C4 grasses to aridity reflect species‐specific strategies in a semiarid savanna

AbstractThe C4 Poaceae are a diverse group in terms of both evolutionary lineage and biochemistry. There is a distinct pattern in the distribution of C4 grass groups with aridity; however, the mechanistic basis for this distribution is not well understood. Additionally, few studies have investigated the functional strategies of co‐occurring C4 grass species for dealing with aridity in their natural environments. We explored the coordination of leaf‐level gas exchange, water use, and morphology among five co‐occurring semiarid C4 grasses belonging to divergent clades, biochemical subtypes, and size classes at three sites along a natural aridity gradient. More specifically, we measured predawn and midday water potential, stomatal conductance, water use efficiency, and photosynthesis. Leaf tissue was also collected for the analysis of stable isotopes of carbon and oxygen as well as for measurement of specific leaf area (SLA) and leaf width. Species differences in responsiveness of stomata to changes in vapor pressure deficit (VPD) were also assessed. It was expected that NAD‐me species would maintain higher rates of photosynthesis, higher water use efficiency, and have more responsive stomata than other co‐occurring species based on observed biogeographic patterns and past greenhouse studies. We found that Aristidoideae and Chloridoideae NAD‐me‐type grasses had greater stomatal sensitivity to VPD, consistent with a more isohydric strategy. However, midgrasses had both greater apparent water access and water use efficiency, regardless of subtype or lineage. PCK‐type species had less responsive stomata and maintained lower levels of photosynthesis with increasing aridity. There were strong interspecific differences in δ13C, leaf width, and SLA; however, these were not significantly correlated with water use efficiency. C4 grasses in our study did not fit discretely into functional groups as defined by lineage, biochemistry, or size class. Interspecific differences, evolutionary legacy, and biochemical pathway are likely to interact to determine water use and photosynthetic strategies of these plants. Control of water loss via highly responsive stomata may form the basis for dominance of certain C4 grass groups in arid environments. These findings build on our understanding of contrasting strategies of C4 grasses for dealing with aridity in their natural environments.

Tracing the orogenic sulfur cycle in the Andes using stable isotope composition of dissolved sulfate in thermal springs

The cycling of sulfur (S) to the upper crust and surface via thermal springs at convergent margins has not been explored outside areas with active arc volcanism, even though subduction plays a key role in the Earth's long-term S cycle. To address this knowledge gap, we analyzed stable sulfur and oxygen isotope compositions (δ34S and δ18O values) of dissolved sulfate (SO42−) in 55 thermal springs from five distinct settings in the Andean orogen. These regions are the Peruvian flat slab and backarc, transition between these two, Argentinian backarc, and Chilean forearc. Although the flat-slab settings had lower SO42− concentrations (<2000 mg/L) compared to the steep-slab settings (<12,700 mg/L), there was no significant relationship between isotope composition of SO42− and slab geometry. The δ34S and δ18O values of SO42− varied widely across the studied areas (+0.2 to +23.5 ‰ and − 3.3 to +16.0 ‰, respectively) and reflected the isotope compositions of local bedrock endmembers from dissolution of marine evaporites (+5 to +25 ‰ and + 10 to +20 ‰, respectively) and oxidation of magmatic and/or hydrothermal S and ore sulfide minerals with variable δ34S (0 to +16 ‰). The δ18O and δ2H values of thermal spring water (−18.5 to −3.3 ‰ and − 141.1 to −23.7 ‰, respectively) were consistent with meteoric precipitation, and in most cases decreased with increasing altitude following precipitation in the Andes. Generally, our isotope results do not support the direct transfer of slab-derived S/SO42− to thermal springs in the investigated settings. Rather, the δ34S and δ18O of SO42− in the thermal springs are a sensitive indicator of local water-rock interactions that remobilize bedrock S originating from a complex orogenic cycle reflecting tectonic uplift, erosion, weathering, and exhumation history across the duration of Andean Mountain building.

Changes in Water Utilization Characteristics of Trees in Forests across a Successional Gradient in Southern China

Elucidating the water utilization strategy of trees during forest succession is a prerequisite for predicting the direction of forest succession. However, the water utilization characteristics of trees in forests across a successional gradient remain unclear. Here, we utilized the hydrogen and oxygen stable isotopes combined with the Bayesian mixed model (MixSIAR) to analyze the water utilization of dominant trees (Pinus massoniana, Castanea henryi, and Schima superba) in forests along a successional gradient in the Dinghushan Biosphere Reserve of China. Furthermore, we determined the primary factor affecting the water utilization of various trees based on variation partitioning analysis and a random forest model. Our results illustrated that in the early-successional forest, the water utilization ratios from shallow soil layers by P. massoniana were significantly lower than that in the mid-successional forest (51.3%–61.7% vs. 75.3%–81.4%), while its water utilization ratios from deep soil layers exhibited the opposite pattern (26.1%–30.1% vs. 9.0%–15.0%). Similarly, the ratios of water utilization from shallow soil layers by C. henryi (18.9%–29.5% vs. 32.4%–45.9%) and S. superba (10.0%–25.7% vs. 29.2%–66.4%) in the mid-successional forest were relatively lower than in the late-successional forest, whereas their water utilization ratios from deep soil layers showed the contrary tendency. Moreover, our results demonstrated that the diverse water utilization of each tree in different successional forests was mainly attributed to their distinct plant properties. Our findings highlight the increased percentage of water utilization of trees from shallow soil layers with forest succession, providing new insights for predicting the direction of forest succession under changing environments.

Stable Water Isotope Signals and Their Relation to Stratiform and Convective Precipitation in the Tropical Andes

AbstractStratiform and convective precipitation are known to be associated with distinct isotopic fingerprints in the tropics. Such rain type specific isotope signals are of key importance for climate reconstructions derived from climate proxies (e.g., stable isotopes in tree rings). Recently, the relation between rain type and isotope signal in present‐day climate has been intensively discussed. While some studies point out the importance of deep convection, other studies emphasize the role of stratiform precipitation for strongly depleted isotope signals in precipitation. Uncertainties arise from observational studies due to data scarcity while modeling approaches with global climate models cannot explicitly resolve convective processes and rely on parameterizations. High‐resolution climate models are particularly important for studies over complex topography and for the simulation of convective cloud formation and organization. Therefore, we applied the isotope‐enabled version of the high‐resolution climate model from the Consortium for Small‐Scale Modeling (COSMOiso) over the Andes of tropical south Ecuador, South America, to investigate the influence of stratiform and convective rain on the stable oxygen isotope signal of precipitation (δ18OP). Our results highlight the importance of deep convection for depleting the isotopic signal of precipitation and increasing its deuterium excess. Due to the opposing effect of shallow and deep convection on the δ18OP signal, the use of a stratiform fraction might be misleading. We therefore propose to use a shallow and deep convective fraction to analyze the effect of rain types on δ18OP.

Groundwater Dynamics in African Endorheic Basins in Arid to Semi-Arid Transition Zones: The Batha Aquifer System, NE Chad

This study investigates the Batha endorheic basin in Chad, situated east of the Lake Chad basin in the arid to semi-arid Sahelian zone. This region has not yet undergone comprehensive geological and hydrogeological studies. More broadly, the transition zone between semi-arid and arid climates has been minimally explored. This research aims to evaluate the resources and dynamics of this multi-layered system using a combined geology-hydrogeology-hydrochemistry-isotopes approach. The multilayer system includes sedimentary layers (Quaternary, Pliocene, and Eocene) over a crystalline basement. A piezometric investigation of the system shows a general SE–NW groundwater, indicating an interconnection between all layers. Hydrochemical analyses identifies four main facies (calcium-bicarbonate, sodium-bicarbonate, sulphate-sodium, and mixed), primarily controlled by water–rock interaction with secondary influences from base-exchange and evaporation. Saturation indices indicate that these waters are close to equilibrium with the calcite-Mg phases, gaylussite and gypsum. Stable isotopes (oxygen-18 and deuterium) categorize groundwater into three groups: ancient water, recent and older meteoric water mixtures affected by evaporation, and mixtures more heavily impacted by evaporation. Tritium contents reveal three groups: current rainwater, modern water, and sub-modern water. These results indicate that ionic and isotopic differentiations cannot be strictly linked to specific layers, confirming the interconnected nature of the Batha system. The observed heterogeneity is mainly influenced by lithological and climatic variations. This study, though still limited, enhances significantly the understanding of the basin’s functioning and supports the rational exploitation of its vital resources for the Batha area’s development. Future investigations to complete the present study are highlighted.