Long-term Storage Stability Research Articles

Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays

As the world has been facing several deadly virus crises, including Zika virus disease, Ebola virus disease, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Coronavirus disease 2019 (COVID-19), lateral flow assays (LFAs), which require minimal equipment for point-of-care of viral infectious diseases, are garnering much attention. Accordingly, there is an increasing demand to reduce the time and cost required for manufacturing LFAs. The current study introduces an equipment-free method of salt-mediated immobilization of nucleic acids (SAIoNs) for LFAs. Compared to general DNA immobilization methods such as streptavidin–biotin, UV-irradiation, and heat treatment, our method does not require special equipment (e.g., centrifuge, UV-crosslinker, heating device); therefore, it can be applied in a resource-limited environment with reduced production costs. The immobilization process was streamlined and completed within 30 min. Our method improved the color intensity signal approximately 14 times compared to the method without using SAIoNs and exhibited reproducibility with the long-term storage stability. The proposed method can be used to detect practical targets (e.g., SARS-CoV-2) and facilitates highly sensitive and selective detection of target nucleic acids with multiplexing capability and without any cross-reactivity. This novel immobilization strategy provides a basis for easily and inexpensively developing nucleic acid LFAs combined with various types of nucleic acid amplification.

Colorimetric paper-based sarcosine assay with improved sensitivity.

This manuscript reports on a simple paper-based bienzymatic colorimetric assay for sarcosine as an important urinary biomarker of prostate cancer. All required assay reagents are pre-deposited on hydrophilic filter paper spots surrounded by a hydrophobic barrier. Sarcosine in the sample solution is selectively oxidized in the presence of sarcosine oxidase (SOx), resulting in the formation of hydrogen peroxide, which is subsequently detected through the horseradish peroxidase (HRP)-catalyzed conversion of the colorless indicator 3,3',5,5'-tetramethylbenzidine (TMB) into its blue-colored oxidation product. By the modification of the paper with positively charged poly(allylamine hydrochloride) (PAH), a linear response to sarcosine between 0 and 10μM and a significant lowering of the limit of detection (LOD) (0.6μM) compared to the unmodified paper substrate (12.6μM) has been achieved. The improvement of the LOD was attributed to the fact that the presence of the polymer limits the enzyme-driven colorimetric reaction to the surface of the paper substrate, resulting in stronger color development. In experiments in artificial urine matrix, the bicarbonate anion was identified as an inhibitor of the colorimetric reaction. This inhibition was successfully eliminated through on-device sample pH adjustments with pH-buffer components pre-deposited onto assay devices. The LOD for sarcosine achieved in artificial urine matrix (2.5μM) is below the 5μM threshold value for this urinary biomarker required for diagnostic purposes. Finally, good selectivity over all 20 natural amino acids and satisfactory long-term storage stability of reagent-modified paper substrates at - 20°C for a period of 50days were confirmed.

Instability of corticotropin during long-term storage- myth or reality?

Corticotropin is notorious for its instability. Whereas several studies have investigated its short-term stability in plasma following venous blood sampling, studies on long-term stability are lacking. Here we investigated the long-term storage stability of corticotropin in ethylenediaminetetraacetic acid containing plasma. Specimens from healthy volunteers (neat, spiked) were stored in polypropylene microcentrifuge tubes with socket screw-caps at-20°C and-70°C for up to one and a half years. Corticotropin in plasma was measured using an Abbott research only immunoassay. Separately, specimens from patients were collected during diagnostic routine testing and stored in polystyrene tubes with push-caps at-20°C for up to 6 years. In these samples corticotropin hormone was measured using the Diasorin corticotropin immunoassay. Storage of specimens at-20°C or-70°C for up to one and a half years showed minimal changes (<11%) in corticotropin levels, while storage of patient samples at-20°C for up to 6 years showed a significant (54%) reduction in corticotropin levels. Corticotropin levels are stable in plasma when stored at-20°C for one and a half years using the Abbott research only assay, but with longer storage time a significant reduction in corticotropin levels can be expected. Once specimens are stored for future corticotropin measurements, one should consider storage time, storage temperature and assay differences.

Carbon Dots‐in‐EuAPO‐5 Zeolite: Triple‐Emission for Multilevel Luminescence Anti‐Counterfeiting

Multilevel luminescence materials have aroused wide attention for their advanced anti-counterfeiting abilities. However, various complicated stimuli factors involved in multilevel luminescence anti-counterfeiting (MlLA) limit the practical applications of such materials. Herein, carbon dots (CDs) are in situ introduced into Eu-substituted AlPO4 -5 zeolite (named CDs@EuAPO-5) via a solvent-free thermal crystallization method, which exhibits triple emissions including pink fluorescence mainly associated with Eu3+ in the zeolite framework, blue fluorescence and green room temperature phosphorescence (RTP) associated with CDs. CDs are uniformly embedded in the EuAPO-5 zeolite matrix. Such composite displays excellent photo-, thermo-, and solvent resistance, as well as long-term storage-stability. Moreover, the triple emissions of the composite only need two kinds of common excitation lights to trigger, without involving other complicated stimuli. A triple-level luminescence anti-counterfeiting (TlLA) label has been built, realizing facile, quick, and advanced luminescence anti-counterfeiting that is hard to copy.

Structural insights into the enhanced thermostability of cysteine substitution mutants of L-histidine decarboxylase from Photobacterium phosphoreum.

Enzymatic amino acid assays are important in physiological research and clinical diagnostics because abnormal amino acid concentrations in biofluids are associated with various diseases. L-histidine decarboxylase from Photobacterium phosphoreum (PpHDC) is a pyridoxal 5'-phosphate-dependent enzyme and a candidate for use in an L-histidine quantitation assay. Previous cysteine substitution experiments demonstrated that the PpHDC C57S mutant displayed improved long-term storage stability and thermostability when compared with those of the wild-type enzyme. In this study, combinational mutation experiments of single cysteine substitution mutants of PpHDC were performed, revealing that the PpHDC C57S/C101V/C282V mutant possessed the highest thermostability. The stabilizing mechanism of these mutations was elucidated by solving the structures of PpHDC C57S and C57S/C101V/C282V mutants by X-ray crystallography. In the crystal structures, two symmetry-related PpHDC molecules form a domain-swapped homodimer. The side chain of S57 is solvent exposed in the structure, indicating that the C57S mutation eliminates chemical oxidation or disulfide bond formation with a free thiol group, thereby providing greater stability. Residues 101 and 282 form hydrophobic interactions with neighboring hydrophobic residues. Mutations C101V and C282V enhanced thermostability of PpHDC by filling a cavity present in the hydrophobic core (C101V) and increasing hydrophobic interactions.

Long-term Storage Stability Assessment of Omega-3-Fatty Acid Emulsified Formulation Containing Micronutrients

Long-term Storage Stability Assessment of Omega-3-Fatty Acid Emulsified Formulation Containing Micronutrients

Magnetic ZIF-8-Based Mimic Multi-enzyme System as a Colorimetric Biosensor for Detection of Aryloxyphenoxypropionate Herbicides.

In the present study, a magnetic mimic multi-enzyme system was developed by encapsulating the aryloxyphenoxypropionate (AOPP) herbicide hydrolase QpeH and alcohol oxidase (AOx) in zeolitic imidazolate framework (ZIF-8) nanocrystals with magnetic Fe3O4 nanoparticles (MNPs) to detect AOPP herbicides. The structural, protein loading capacity and loading ratio, porosity, and magnetic properties of QpeH/AOx@mZIF-8 were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen sorption, and vibrating sample magnetometry. An AOPP herbicide colorimetric biosensor made with QpeH/AOx@mZIF-8 had the highest sensitivity toward quizalofop-P-ethyl (QpE) with a limit of detection of 8.2 μM. This system was suitable to detect two other AOPP herbicides, including fenoxaprop-P-ethyl (FpE) and haloxyfop-P-methyl (HpE). The practical application of the biosensor was verified through quantitative analysis of QpE residues in industrial wastewater and field soils. Furthermore, QpeH/AOx@mZIF-8 exhibited excellent long-term storage stability (at least 50 days), easy separation by magnet, and reusability (at least 10 cycles), supporting its promising role in simple and low-cost detection of AOPP herbicides in real environmental samples.

Development, characterization and in vitro–in vivo evaluation of Farnesol loaded niosomal gel for applications in oral candidiasis treatment

ObjectivesThe aim of the study was to formulate and characterize the farnesol loaded niosomes comprising gel formulation and perform their in vitro–in vivo evaluation for applications in the treatment of oral candidiasis infections. MethodsVarious gelling systems were evaluated for their rheological and stability properties. The formulation was statistically optimized using experimental design method (Box-Behnken). Transmission electron microscopy (TEM) and Atomic force microscopy (AFM) were used to observe the niosomal surface morphology. Centrifugation method and dialysis method were used to find out the % entrapment efficiency (%EE) and in-vitro release of Farnesol, respectively. In-vitro antifungal effect and cell biocompatibility of the Farnesol loaded niosomal gel was also performed using Candida albicans (C. albicans) as the model organism and epithelial cell line (SW480) by MTT cytotoxicity assay. In-vivo skin irritation test was performed on rabbit skin. Key findingsFarnesol loaded niosomes were integrated into polymeric gel solution. The optimized formulation demonstrated acceptable % EE (>80%) and an optimum particle size (168.8 nm) along with a sustained release and a long-term storage stability for up to a period of 6 months. TEM and AFM observations displayed a spherical niosome morphology. Farnesol niosomal gel showed a higher antifungal efficacy, ex-vivo skin permeation and deposition in comparison to plain farnesol solution. The niosomal gel also showed negligible cytotoxicity to normal cells citing biocompatibility and was found to be non-toxic and non-irritant to rabbit skin. ConclusionsThis novel niosome loaded gel-based formulation could make the oral candidiasis healing process more efficient while improving patient compliance. With the optimized methodology used in this work, such formulation approaches can become an efficient, industrially scalable, and cost-effective alternatives to the existing conventional formulations.

High-Payload Buccal Delivery System of Amorphous Curcumin-Chitosan Nanoparticle Complex in Hydroxypropyl Methylcellulose and Starch Films.

Oral delivery of curcumin (CUR) has limited effectiveness due to CUR’s poor systemic bioavailability caused by its first-pass metabolism and low solubility. Buccal delivery of CUR nanoparticles can address the poor bioavailability issue by virtue of avoidance of first-pass metabolism and solubility enhancement afforded by CUR nanoparticles. Buccal film delivery of drug nanoparticles, nevertheless, has been limited to low drug payload. Herein, we evaluated the feasibilities of three mucoadhesive polysaccharides, i.e., hydroxypropyl methylcellulose (HPMC), starch, and hydroxypropyl starch as buccal films of amorphous CUR–chitosan nanoplex at high CUR payload. Both HPMC and starch films could accommodate high CUR payload without adverse effects on the films’ characteristics. Starch films exhibited far superior CUR release profiles at high CUR payload as the faster disintegration time of starch films lowered the precipitation propensity of the highly supersaturated CUR concentration generated by the nanoplex. Compared to unmodified starch, hydroxypropyl starch films exhibited superior CUR release, with sustained release of nearly 100% of the CUR payload in 4 h. Hydroxypropyl starch films also exhibited good payload uniformity, minimal weight/thickness variations, high folding endurance, and good long-term storage stability. The present results established hydroxypropyl starch as the suitable mucoadhesive polysaccharide for high-payload buccal film applications.

Synthesis and characterisation of dimeric triphenylmethane water-soluble dyes for high-speed inkjet printing

Three dimeric-triphenylmethane dyes were designed and synthesised for enhancing the rigidity of triphenylmethane moiety and three water-soluble dyes were prepared from them in order to manufacture water-based inks for high-speed ink-jet printing technology. The geometrical characteristics of the synthesised dyes were investigated by carrying out density functional theory calculations. Because of their highly twisted chemical structures and hydrophilic substituents, the synthesised dyes showed efficiently enhanced water solubility when formulated into water-based inks. All the prepared dyes exhibited superior molar extinction coefficients and photo stability than the commercially available acid blue dye. The prepared water-based inks showed the excellent long-term storage stability by optimising the physical and rheological properties of the inks. Moreover, they exhibited good ejection performance and well-ordered pattern arrays when applied to high-speed inkjet printing tests.

PH-triggered small molecule nano-prodrugs emulsified from tryptamine-cinnamaldehyde twin drug for targeted synergistic glioma therapy

Chemotherapy fails to achieve an ideal gliomas therapy due to the limited delivery of chemotherapeutics across the blood brain barrier (BBB), difficult accumulation of drugs in the gliomas area, and off-target toxicity. Herein, the pH-triggered small molecule nano-prodrugs (Try-CA-NPs) emulsified from hydrophobic tryptamine (Try)-cinnamaldehyde (CA) twin drug were successfully prepared through a facile method. Try-CA-NPs exhibited long-term storage and circulation stability. Furthermore, liposoluble Try-CA-NPs could easily cross BBB and efficiently accumulate in brain, selectively target to gliomas cells via Try-mediated cellular uptake, and enhance cytotoxicity through intracellular pH-triggered endosomal escape and efficient drug release, and synergistic effect between CA and Try, therefore achieving the complete destruction of SH-SY5Y multicellular spheroids (MCs). Thus, the pH-triggered small molecule nano-prodrugs emulsified from Try-CA twin drug have the great potential for clinically targeted synergistic glioma therapy.

Stainless steel 304 needle electrode for precise glucose biosensor with high signal-to-noise ratio

While accuracy and precision of human serum glucose measurements play a crucial role in determining treatments targeting glycemic control, electrochemical glucose meters widely used for blood sugar monitoring, have been facing issues of inaccuracy, making their application in medical decision-making difficult. Herein, we report a new amperometric glucose biosensor with extreme precision based on a stainless steel 304 (SS304) needle modified with glucose oxidase (GOx), chitosan, and Nafion. GOx and chitosan are immobilized on the surface of the SS304 needle and then covered with the Nafion layer. We focused on reducing electrochemical background signals and securing high signal-to-noise ratios associated with the electrode material, in contrast to most previous studies focusing on detection sensitivity enhancement. The SS304 needle was employed as a sensing electrode with H2O2, a product of GOx-catalysed oxidation reaction of glucose, owing to its convenient electrical properties with extremely shallow non-faradaic background currents under electrochemical conditions. We confirmed that the SS304 needle immobilized with glucose oxidase shows a distinct increase in current upon the addition of glucose at −0.10 V (vs. a saturated calomel electrode). The amperometric signal from the modified SS304 was specific to the serum glucose level without notable interference. It exhibits a strict linear correlation in the diabetic sugar level of 1–20 mM (y = 0.205x, R2 = 1.000) with an outstanding reproducibility (RSD = 4.4–6.8 %) and long-term storage stability. These results demonstrated that the SS304 electrode possesses great potential for electroanalytical biosensors based on the H2O2 content.

Brightly luminescent (NH4)xCs1-xPbBr3 quantum dots for in vitro imaging and efficient photothermal ablation therapy

Herein, we report for the first time a facile strategy for the highly efficient (NH4)xCs1-xPbBr3 quantum dots (QDs). By modulating the amount of ammonium, (NH4)xCs1-xPbBr3 QDs with different photoluminescence (PL) quantum yields (QY) were synthesized. The results of X-ray diffraction and X-ray photoelectron spectroscopy showed that the crystal structure of (NH4)xCs1-xPbBr3 was altered by incorporation of NH4+ cations into the CsPbBr3 lattice. The (NH4)xCs1-xPbBr3 QDs showed enhanced PL QY, higher photostability, and long-term storage stability compared to CsPbBr3 QDs. Furthermore, (NH4)xCs1-xPbBr3 QDs could be conjugated with a photothermal dye (IR780) via a one-pot reaction using poly(styrene-co-maleic anhydride) and IR780-MPTS. To the best of our knowledge, the present work is the first attempt integrating perovskite QDs and phototherapeutic molecules into one system (abbreviated as PQD-IR780), demonstrating good water dispersibility and high photothermal conversion efficiency of 57.85%. In vitro experiments performed to examine subcellular uptake showed high fluorescence brightness was observed in HeLa, B16F1, and HepG2 cancer cells cultured with PQD-IR780. The results indicate that the internalization mechanism for uptaking of PQD-IR780 inside HeLa cells is energy-dependent and caveolin-mediated endocytosis. The in vitro cell viability assays and photothermal therapy revealed that PQD-IR780 showed good biocompatibility and can induce hyperthermia upon laser irradiation.

High-performance CdSe/CdS@ZnO quantum dots enabled by ZnO sol as surface ligands: A novel strategy for improved optical properties and stability

Colloidal semiconductor quantum dots (QDs) have attracted great attention in field of optoelectronic devices. However, the surface organic ligands of QDs involving in optical instability and limited passivation remain challenging, thus bringing additional difficulties in practical application. Here, the high-performance CdSe/CdS@ZnO QDs were successfully prepared by ZnO sol as surface ligands. Importantly, this kind of CdSe/CdS@ZnO QDs maintained the typical advantages of conventional QDs, such as superior monodispersity and long-term storage stability in solution. Because of the effective surface passivation provided by inorganic sol ligands and the deionization effect that electron transferring from surface state of QDs to ZnO acceptor, CdSe/CdS@ZnO QDs showed the remarkably enhanced photostability and biexciton lifetime, as well as further suppressed Auger process and PL blinking. Meanwhile, the CdSe/CdS@ZnO QDs demonstrated PL thermal stability much higher than that of pristine CdSe/CdS QDs under heating to 100 °C, which was efficiently improved 3 times that from ~20% to ~63% of their initial emission intensity. Moreover, a stable amplified spontaneous emission process was observed in CdSe/CdS@ZnO QDs film and still retained a lower ASE threshold (~28 μJ cm−2). Finally, we successfully fabricated a high-performance vertical cavity surface-emitting laser based on CdSe/CdS@ZnO QDs, which presented spatially directional single-mode lasing output with an ultralow threshold of ~3.3 μJ cm−2. We believe that this work could not only enrich the family of surface ligands passivated semiconductor QDs but also provide a novel strategy towards constructing stable and functionalized colloidal nanomaterials for all-inorganic optoelectronic devices.

Plant protein-based antioxidant Pickering emulsions and high internal phase Pickering emulsions against broad pH range and high ionic strength: Effects of interfacial rheology and microstructure

The present work aimed to explore the stability mechanism of Pickering emulsions (PEs) and high internal phase PEs (HIPPEs) to resist environmental stresses (i.e., pH and ionic strength) via altering the interfacial rheology and microstructure. The storage, centrifugal, oxidative stabilities and interfacial properties of PEs and HIPPEs stabilized by pea protein isolate-high pectin-epigallocatechin gallate (PPI-HMP-EGCG) complex were investigated. The compact and dense viscoelastic interfacial layers, appropriate gel-like elastic network structure and apparent viscosity allowed PEs and HIPPEs to have excellent long-term storage stability, the best centrifugal and oxidative stabilities at pH 3.5 and 0 mmol/L NaCl. As PEs and HIPPEs at neutral and alkaline conditions, the centrifugal and oxidative stabilities of PEs and HIPPEs reduced, which was ascribed to the insufficient number of complexes, leading to the formation of the weak interfacial layer and the decrease of apparent viscosity and viscoelasticity. As NaCl concentration increased to 1000 mmol/L, the oil droplets were not completely surrounded by the interfacial layer with weak viscoelastic response, which resulted in the reduction of centrifugal and oxidative stabilities. These findings are useful to broaden the application of antioxidant PEs and HIPPEs in the food industry under a wide range of pH and ionic strengths.

Evaluation of Long-term Storage Stability of Antimicrobial Agents, Antiviral Agents, and Sodium Chloride Eye Drops Prepared in the Hospital

Evaluation of Long-term Storage Stability of Antimicrobial Agents, Antiviral Agents, and Sodium Chloride Eye Drops Prepared in the Hospital

Multifunctional Crosslinking-Enabled Strain-Regulating Crystallization for Stable, Efficient α-FAPbI3 -Based Perovskite Solar Cells.

α-Formamidinium lead triiodide (α-FAPbI3 ) represents the state-of-the-art for perovskite solar cells (PSCs) but experiences intrinsic thermally induced tensile strain due to a higher phase-converting temperature, which is a critical instability factor. An in situ crosslinking-enabled strain-regulating crystallization (CSRC) method with trimethylolpropane triacrylate (TMTA) is introduced to precisely regulate the top section of perovskite film where the largest lattice distortion occurs. In CSRC, crosslinking provides in situ perovskite thermal-expansion confinement and strain regulation during the annealing crystallization process, which is proven to be much more effective than the conventional strain-compensation (post-treatment) method. Moreover, CSRC with TMTA successfully achieves multifunctionality simultaneously: the regulation of tensile strain, perovskite defects passivation with an enhanced open-circuit voltage (VOC = 50mV), and enlarged perovskite grain size. The CSRC approach gives significantly enhanced power conversion efficiency (PCE) of 22.39% in α-FAPbI3 -based PSC versus 20.29% in the control case. More importantly, the control PSCs' instability factor-residual tensile strain-is regulated into compression strain in the CSRC perovskite film through TMTA crosslinking, resulting in not only the best PCE but also outstanding device stability in both long-term storage (over 4000 h with 95% of initial PCE) and light soaking (1248 h with 80% of initial PCE) conditions.

Conjugation with inulin improves the environmental stability of haloalkane dehalogenase DhaA

Haloalkane dehalogenase DhaA catalyzes the hydrolytic cleavage of carbon-halogen bonds and produces alcohol, a proton and a halide. However, DhaA suffers from the poor environmental stability, such as sensitivity to high temperature, low pH, hypersaline and organic solvent. In order to improve the environmental stability of DhaA, DhaA was covalently conjugated with inulin, a hydrophilic polysaccharide in the present study. Each DhaA was averagely conjugated with 7∼8 inulin molecules. The conjugated inulin could form a hydration layer around DhaA, which increased the conformational rigidity and decreased the entropy of the enzyme. Conjugation of inulin maintained 75.5 % of the enzymatic activity of DhaA and slightly altered the structure of DhaA. As compared with DhaA, the conjugate (inu-DhaA) showed slightly different kinetic parameters (Km of 2.9 μmol/L and Kcat of 1.0 s−1). Inulin conjugation could delay the structural unfolding and/or slow the protonation process of DhaA under undesirable environment, including the long-term storage, low pH, hypersaline and organic solvent stability. As a result, the environmental stability of DhaA was markedly increased upon conjugation with inulin. Thus, inulin conjugation was an effective approach to enhance the environmental stability of DhaA.

Novel SeS2-loaded Co MOF with Au@PANI comprised electroanalytical molecularly imprinted polymer-based disposable sensor for patulin mycotoxin

An SeS2-loaded Co MOF and Au@PANI nanocomposite comprising the base matrix of the electrode was developed with electropolymerized molecularly imprinted polymer (MIP) consisting of p-aminobenzoic acid (PABA) and patulin (PT) to detect PT molecules based on the PT imprinted cavities. SeS2@Co MOF and Au@PANI were synthesized using hydrothermal synthesis and interfacial polymerization strategies, respectively. A suitable functional monomer to fabricate the MIP platform was selected using the density functional theory (DFT/M06-2X method). Higher electrochemical active surface area (0.985 cm2 which is 6.99 times higher than the bare SPE) and a lower charge transfer resistance (Rct = 27.8 Ω) at the MIP/Au@PANI/SeS2@Co MOF electrode was achieved based on the higher number of adsorptive sites and enhanced conductivity (electron transfer rate constant (ks = 3.24 × 10−3 s−1) of the sensing platform. The fabricated MIP sensor performance was studied in 10 mM PBS (pH = 6.4), where an improved detection limit (0.66 pM) for PT and a broad logarithmic linear dynamic range (0.001–100 nM) were both observed. The sensor possessed higher selectivity (Imprinting factor = 15.4 for PT), excellent reusability (%RSD of 10 cycles = 2.49%), high storage stability (6.7% lost after 35 days), and robust reproducibility (%RSD = 3.22%) The as-prepared MIP-based PT sensor was applied to detect PT in a real-time apple juice sample (10% diluted with PBS) with a recovery % ranging from 94.5 to 106.4%. The proposed sensor possesses great advantages in terms of cost-effectiveness, providing a simple detection strategy for long-term storage stability, and reversible cycle measurements.

Solid encapsulation of lauric acid into “empty” V-type starch: Structural characteristics and emulsifying properties

Lauric acid was introduced into “Empty” V-type starch using a solid encapsulation method. The structural characteristics and emulsifying properties of the starch-fatty acid complex (SFAC) were explored as a function of the complexing temperature. X-ray diffraction and differential scanning calorimetry confirmed that SFAC was mainly composed of type-I amylose inclusion complexes. Contact angle measurements revealed that the hydrophobic properties of SFAC were closely related to the temperature-regulated complex index. The particle size range of SFAC gradually increased as the complexing temperature increased. The SFAC-stabilized Pickering emulsion at c of 5% and Φ of 40–60% possessed a small droplet size and long-term storage stability for up to 30 days, resulting from the formation of a gel-like network. This study provides new insight into the design of hydrophobic modified starch as a novel and multifunctional emulsifier and is of great help in the development of starch-based Pickering emulsion gels.