Stabilization Process Research Articles

A Comprehensive Review on Mine Tailings as a Raw Material in the Alkali Activation Process

The mining industry generates vast quantities of mine tailings on an annual basis. However, due to their limited economic value, a significant portion of these tailings are deposited close to mining sites, often underwater. The principal environmental apprehensions associated with mine tailings revolve around their elevated levels of heavy metals and sulfidic minerals. The oxidation of these sulfidic minerals can lead to the formation of acid mine drainage, which in turn releases heavy metals into nearby water systems. The effective management of tailing dams requires substantial financial investments for their construction and meticulous control. Consequently, a pressing need exists for stable, sustainable, and economically viable management approaches. One promising method for addressing mine tailings is through alkali activation, a technique that serves as a stabilization process. This approach yields robust, concrete-like structures by utilizing raw materials abundant in aluminum and silicon, which conveniently constitute the primary components of mining residues. This comprehensive review outlines the research on utilizing alkali activation for mine tailings. It delves into the reactivity and chemical attributes of diverse minerals. Numerous mine tailings exhibit an inadequate level of reactivity under alkaline conditions, so various pre-treatment methodologies and their impacts on mineralogy are meticulously explored.

DEVELOPMENT OF THE E-COMMERCE MARKET IN UKRAINE IN THE CONTEXT OF MODERN CHALLENGES

Over the past few decades, the use of e-commerce in business has increased significantly. More and more companies that used to focus exclusively on offline operations are beginning to consider e-commerce as a promising area of strategic development. As a result, studying the state of the e-commerce market in Ukraine, as well as trends and barriers to its development, will allow to develop an understanding of the peculiarities of the functioning and growth of business entities in the context of the Ukrainian online environment. In this regard, it is worth paying special attention to the study of the specifics of the development of the e-commerce market as a strategic recovery of the Ukrainian economy. The subject of the study is the process of development of the Ukrainian e-commerce market in the conditions of war. The purpose is to research and analyse the state of the e-commerce market in Ukraine in the context of growing instability caused by the war, as well as to identify key issues and trends in the development of the e-commerce system in an unstable and changing environment. The questions of this study require the use of a combination of general scientific and modern research methods, among which the most important are the method of system analysis (when studying the approaches and points of view of Ukrainian and Western scientists), economic and statistical analysis (to determine the trends in the development of e-commerce in Ukraine), analysis and synthesis (when studying the types and models of e-commerce), graphic analysis (to visualize a number of theoretical and practical provisions of the article). The article examines the state of the e-commerce market in Ukraine during the war. It finds that until 2022 there was a significant increase in the volume and growth rate of the e-commerce market. With the outbreak of the war, the market experienced a dramatic drop in volume, but from 2023 significant stabilisation processes took place. The importance of further development of the e-commerce market not only during the war, but also to overcome its consequences during the post-war recovery period is determined. Conclusions. Ukrainian e-commerce is experiencing high growth rates. The barriers to entering the online market are relatively low and the benefits of e-commerce for users and entrepreneurs are tangible. However, at the current stage, the Ukrainian e-commerce market has many unresolved issues that hinder its development. If the key issues are effectively addressed, the development of e-commerce in Ukraine will have a future.

Evolutionary stages of active to vegetated coastal transgressive dunefields in the San Matías Gulf coast, Argentina

The understanding of how active aeolian coastal dunes transform into fully vegetated forms, and the specific dune configurations that emerge, particularly within coastal dune systems, is limited. This study examines the changes in dune morphology associated with vegetation growth within transgressive dunefields in NE Patagonia, Argentina. The dunefields are located along the northern coast of the San Matías Gulf, ranging from relatively small to large, and dominantly active in the past ∼80 years. Satellite and aerial images were analyzed using GIS procedures to calculate vegetation cover changes and dune migration rates and identify different dune types and their evolutionary stages of fixation. Five stages were defined in relation to vegetation cover and dune shapes and patterns, from bare sand to fully vegetated dunes. Transverse or barchanoid ridges (sometimes with aklé [fish scale], or chaotic patterns) convert to partially vegetated sub-parabolic dunes and finish as parabolic dunes. In other cases, the dunes may convert to reticulate, aklé, or chaotic patterns, completely vegetated. Various stages span a continuous range from unvegetated to fully vegetated dunes across a wide array of timeframes. In some cases, it was possible to recognize changes from stage one to five in ∼20 years. Where the initial transverse dune is largely maintained during the stabilization process a distinctive pattern named “gusanos” (worms) was identified. The vegetated surface measured in some areas changed from 11 % to 51 % vegetation cover between 1961 and 2022, with migration rates decreasing from 9.53 m/year to zero between 1991 and 2020. The increase in vegetation growth also coincides with higher temperatures, weaker winds and decreasing grazing livestock within this Patagonia area. The recorded processes are similar to coastal dune and vegetation changes in some semiarid regions from the Southern Hemisphere between 30 and 60° S, especially in South Australia.

Improvement of organic carbon recovery from wastewater by changing water temperature in high-rate contact stabilization process

Improvement of organic carbon recovery from wastewater by changing water temperature in high-rate contact stabilization process

Enhanced CO2 photoreduction to CH4 via *COOH and *CHO intermediates stabilization by synergistic effect of implanted P and S vacancy in thin-film SnS2

Reduction of CO2 to value-added hydrocarbons through artificial photosynthesis is one of the way to address the energy crisis and climate change issues. It is known that lowering the activation energy of CO2 molecules on the photocatalyst surface and key intermediates is crucial in photocatalytic CO2 reduction. Herein, we present phosphorus-implanted 20-nm SnS2 continuous thin film with sulfur vacancies (i.e., SV-SnS2:P where P substitutes on S sites). The fabrication process involves thermal evaporation, post-sulfurization, and ion implantation. Our gas-phase photocatalytic experiments show an enhanced and selective CO2 photoreduction to CH4 with a yield of 0.13 µmol cm−2 and selectivity of 92 % under solar-light irradiation for 4 h over an optimal ∼4.5 % P and ∼16 % SV. Experimental observations, conducted through X-ray absorption near edge, in situ near ambient pressure X-ray photoelectron, and in situ Fourier transform infrared spectroscopies, along with first-principle density functional theory calculations. Results reveal that P dopant is significantly affected by nearby SV via local charge density transfer from P to the nearest Sn and next-nearest S neighbor atoms, consequently, leads to the stabilization of *COOH and *CHO intermediates, where asterisks stand for P as the active site. Our results demonstrate how active site modulation, without using precious co-catalysts, plays a crucial role in intermediate stabilization in a wireless photocatalysis process.

Imaging simulation and restoration for a mobile-based long-focus camera with optical stabilization

Image stabilization technology of the mobile-based long-focus camera is an influential factor that limits the imaging quality. Among them, the periscope lens system is a common choice and is capable of compensating for the external vibration by rotating its prism. To further investigate the impact of residual vibration during the optical image stabilization process, we propose an imaging simulation method to quantify the degradation and construct the corresponding dataset for training. We decompose the degradation into translation perpendicular to the optical axis on the image plane and defocus parallel to the optical axis. The defocus blur pattern is modified to better reproduce the spatially variant deterioration caused by defocus. Constructing synthetic datasets through our proposed imaging simulation method significantly reduces the cost of training the post-processing network while enhancing the imaging quality. Extensive experiments demonstrate the accuracy of our proposed method and the superiority of our synthetic dataset.

Synthesis and Antibacterial Properties of Silver Nanoparticles Derived from Cardiospermum halicacabum Leaves

This study investigates the green synthesis of silver nanoparticles (AgNPs) using Cardiospermum halicacabum leaf extract, which acts as both a reducing and stabilizing agent. The biosynthesis approach is eco-friendly and leverages the phytochemical constituents of Cardiospermum halicacabum. Characterization of the synthesized AgNPs was performed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR), confirming the formation of nanoparticles with desired properties. SEM analysis revealed the morphology and size distribution of the AgNPs, while XRD provided insights into their crystalline nature, and FTIR identified functional groups involved in the reduction and stabilization processes. Despite the successful synthesis of AgNPs, antimicrobial assays indicated that these nanoparticles did not exhibit significant antibacterial activity against tested microorganisms such as Escherichia coli and Staphylococcus aureus. The results suggest that the synthesized AgNPs require further optimization to enhance their antimicrobial efficacy. This study underscores the potential of Cardiospermum halicacabum in green nanoparticle synthesis and highlights the need for continued research to understand the factors influencing the antimicrobial properties of biosynthesized AgNPs, aiming to improve their effectiveness for clinical and industrial applications.

Mn loading on montmorillonite surfaces for Cd stabilization: Insights from density functional theory calculations and surface complexation modeling

The latest works have been devoted to the stabilization mensuration for heavy metals and indicate that clay minerals can promote Cd(II) precipitation by favoring the retention of Mn(II). The assessment however has been tempered due to lacking the information about the molecular-level surface complexation structure and Cd nucleation process on clay surfaces. In this study, microscopic mechanisms for adsorption and stabilization of Cd at montmorillonite interfaces with or without Mn loading were leveraged by combining surface complexation model (SCM) evaluations and density functional theory (DFT) calculations. Mn(II) substitution resulted in increases in surface acidity equilibrium constant (pKa) by about 1 unit and complexation constant (lgK(SOCd+)) of Cd(II) by about 0.15 units at clay surface, and Mn(II) adion can provide extra active sites (i.e., OH− groups) for complexing Cd(II) via hydrolysis. DFT calculations revealed Mn(II) and Cd(II) adions bind on base surfaces by isomorphic substitutions through weak long-range interactions, whereas on edge surfaces by surface complexation with strong but short-range connections. Adsorption energy calculations and electrostatic distribution showed heterogenous nucleation with subsequent cations on clay surfaces was thermodynamically favored, the stabilization process underwent the steps of the adsorption-hydrolysis-precipitation. The derived results provide a quantitative basis for understanding the precipitation and heterogenous nucleation of cations on clay surfaces in surficial environments.

Reference Ranges for Arterial Oxygen Saturation, Heart Rate, and Cerebral Oxygen Saturation during Immediate Postnatal Transition in Neonates Born Extremely or Very Preterm

ObjectiveTo define percentile charts for arterial oxygen saturation (SpO2), heart rate (HR), and cerebral oxygen saturation (crSO2) during the first 15 minutes after birth in neonates born very or extremely preterm and with favorable outcome. Study designWe conducted a secondary-outcome analysis of preterm neonates included in the COSGOD III trial with visible cerebral oximetry measurements and with favorable outcome, defined as survival without cerebral injuries until term age. We excluded infants with inflammatory morbidities within the first week after birth. SpO2 was obtained by pulse oximetry, and electrocardiogram or pulse oximetry were used for measurement of HR. CrSO2 was assessed with near-infrared spectroscopy. Measurements were performed during the first 15 minutes after birth. Percentile charts (10th to 90th centile) were defined for each minute. ResultsA total of 207 preterm neonates with a gestational age of 29.7 (23.9-31.9) weeks and a birth weight of 1200 (378-2320) grams were eligible for analyses. The 10th percentile of SpO2 at minute two, five, ten and 15 was 32%, 52%, 83% and 85%, respectively. The 10th percentile of HR at minute two, five, ten and 15 was 70bpm, 109bpm, 126bpm and 134bpm, respectively. The 10th percentile of crSO2 at minute two, five, ten and 15 was 15%, 27%, 59% and 63%, respectively. ConclusionsThis study provides new centile charts for SpO2, HR, and crSO2 for extremely preterm neonates with favorable outcome. Implementing these centiles in guiding interventions during the stabilization process after birth might help to more accurately target oxygenation during postnatal transition period.

Enhancing spinnability and properties of carbon fibers through modification of isotropic coal tar pitch precursor

Producing general-purpose carbon fibers (GPCFs) with excellent properties using an economical raw material such as coal tar pitch (CTP) holds significant importance. To synthesize carbon fibers, the spinnability of precursor material plays an important role. However, the CTP impedes flowability during the spinning process due to possessing high molecular weight complex aromatic structures. To address this limitation, CTP was blended with varying proportions of petroleum pitch (5, 10 and 20 %) and spinnability and flow behavior were analyzed using Rheology measurements. The distinct constitutional properties of petroleum pitch also contributed to alterations in both the stabilization and carbonization processes. Carbon fibers produced from 10PP showed the highest tensile strength and modulus of ∼784 MPa and ∼47 GPa, respectively. The impact of oxidative and carbonization treatments on the resultant microstructural and mechanical properties of carbon fibers was thoroughly examined using various techniques such as FTIR, XPS, XRD, SEM, Raman and UTM. The results showed that the composition of petroleum pitch influenced the stabilization of fibers which eventually affected the carbonization process and resultant properties of carbon fibers. This study offers an understanding of the connection between the precursor's composition, stabilization conditions, and mechanical properties of the resultant carbon fibers.

Kinetic analysis and back propagation neural network model for shelf-life estimation of stabilized rice bran

To establish a general model for predicting shelf-life of stabilized rice bran, different samples with varying residual enzyme activity, water activity (aw), and packaging materials were used, and hot air assisted-radio frequency (HA-RF) heating was applied as stabilization method. Free fatty acids (FFA) were recorded during storage at different temperatures and kinetic analysis was conducted to compare the effects of different conditions on rice bran shelf-life. FFA content increased rapidly during storage, and higher aw and lipase (LA) activity corresponded to a higher FFA generation rate. Only the increase in FFA of samples with low LA activity (≤30 mg/g) and aw (≤0.3) conformed to Zero-order model, while that of other samples conformed to Fractional Conversion model. For the inhibitory effect of packaging materials on the generation of FFA, BOPP/Kraft-paper/PE > PA/PE > PET/PE > LDPE. A back propagation neural network (BPNN) model was also constructed to predict rice bran shelf-life, which employing LA activity, aw, packaging materials, and storage temperature as input parameters. The BPNN model trained by Levenberg–Marquardt achieved the best prediction performance, with coefficient of determination (R2) of 0.996 and root mean square error (RMSE) of 2.077. Rice bran packaged by BOPP/Kraft-paper/PE with LA activity of 20 mg/g and aw of 0.2 had the longest shelf-life of 147 d at 25 °C. This model is of great help in predicting the shelf-life of stabilized rice bran and guiding the development of rice bran stabilization process.

Effect of MnO2 Nanoparticles Stabilized with Cocamidopropyl Betaine on Germination and Development of Pea (Pisum sativum L.) Seedlings.

This study aimed to synthesize, characterize, and evaluate the effect of cocamidopropyl betaine-stabilized MnO2 nanoparticles (NPs) on the germination and development of pea seedlings. The synthesized NPs manifested as aggregates ranging from 50-600 nm, comprising spherical particles sized between 19 to 50 nm. These particles exhibited partial crystallization, indicated by peaks at 2θ = 25.37, 37.62, 41.18, 49.41, 61.45, and 65.79°, characteristic of MnO2 with a tetragonal crystal lattice with a I4/m spatial group. Quantum chemical modelling showed that the stabilization process of MnO2 NPs with cocamidopropyl betaine is energetically advantageous (∆E > 1299.000 kcal/mol) and chemically stable, as confirmed by the positive chemical hardness values (0.023 ≤ η ≤ 0.053 eV). It was revealed that the interaction between the MnO2 molecule and cocamidopropyl betaine, facilitated by a secondary amino group (NH), is the most probable scenario. This ascertain is supported by the values of the difference in total energy (∆E = 1299.519 kcal/mol) and chemical hardness (η = 0.053 eV). These findings were further confirmed using FTIR spectroscopy. The effect of MnO2 NPs at various concentrations on the germination of pea seeds was found to be nonlinear and ambiguous. The investigation revealed that MnO2 NPs at a concentration of 0.1 mg/L resulted in the highest germination energy (91.25%), germinability (95.60%), and lengths of roots and seedlings among all experimental samples. However, an increase in the concentration of preparation led to a slight growth suppression (1-10 mg/L) and the pronounced inhibition of seedling and root development (100 mg/L). The analysis of antioxidant indicators and phytochemicals in pea seedlings indicated that only 100 mg/L MnO2 NPs have a negative effect on the content of soluble sugars, chlorophyll a/b, carotenoids, and phenols. Conversely, lower concentrations showed a stimulating effect on photosynthesis indicators. Nevertheless, MnO2 NPs at all concentrations generally decreased the antioxidant potential of pea seedlings, except for the ABTS parameter. Pea seedlings showed a notable capacity to absorb Mn, reaching levels of 586.5 μg/L at 10 mg/L and 892.6 μg/L at 100 mg/L MnO2 NPs, surpassing the toxic level for peas according to scientific literature. However, the most important result was the observed growth-stimulating activity at 0.1 mg/L MnO2 NPs stabilized with cocamidopropyl betaine, suggesting a promising avenue for further research.

Effect of stabilization and carbonization treatment on the chemical and physical transformation of polyacrylonitrile (PAN) based electrospun carbon nanofibers

The present study delves into the production of carbon nanofibers (CNFs) utilising electrospun polyacrylonitrile (PAN) nanofiber mats, with a specific focus on the influence of oxidative stabilisation and carbonisation treatments. This research aims to thoroughly understand how variations in stabilisation time and temperature, as well as carbonisation temperature, impact the CNFs properties. These properties include fiber size, morphology, chemical and crystal structure transformation, thermal behaviour, and surface characteristics. Our methodology involved a detailed examination of the thermal treatment processes, where we observed a significant decrease in fiber size, though the surface morphology of the fibers remained largely unaffected. We employed Fourier-transform infrared (FTIR) spectroscopy to track the transformation of nitrile groups in PAN to imine groups, which indicated the progression of cyclisation reactions. Complementary analyses through differential scanning calorimetry (DSC) confirmed a high degree of these reactions, particularly at stabilisation temperatures extending to 250 °C and beyond. The cyclisation process was found to be complete during the carbonisation phase, at temperatures reaching 450 °C and above. Further, x-ray diffraction (XRD) patterns offered insight into the changes in the crystal structure, particularly in the packing of the d(002) spacing because of the stabilisation and carbonisation processes. This findings from this study not only elucidate the intricate process of CNFs production from electrospun PAN nanofiber mats but also highlight the critical factors that influence their final properties. These insights are invaluable for the development of advanced CNFs with tailored properties suitable for a range of applications, including but not limited to energy storage, electronics, and as supporting materials in various technological domains.

Highly Stable Heating Fibers of Ti3C2Tx MXene and Polyacrylonitrile via Synergistic Thermal Annealing.

Nanohybrid assemblies provide an effective platform for integrating the intrinsic properties of individual components into microscale fibers. In this study, a novel approach for creating mechanically and environmentally stable MXene fibers through the synergistic assembly of MXene and polyacrylonitrile (PAN), is introduced. Unlike fibers generated via a conventional stabilization process, which relies on air-based stabilization to transform the PAN molecules into ring structures fundamental to carbon fibers, the hybrid fibers are annealed in an Ar atmosphere. This unique approach suggests MXene can serve as an oxygen provider that is essential for stabilizing PAN. As a result, significantly improved interfiber compactness is achieved and the oxidation stability of MXene is enhanced under atmospheric conditions. The resulting fibers exhibit exceptional stability, even after extended exposure to high humidity and elevated temperatures. This highlights the suitability of the thermally annealed MXene-PAN (T-MX-PAN) fibers as robust electric heating elements. Notably, these fibers consistently generate heat over 1800 bending cycles. When integrated into fabrics, they demonstrate the capability to generate sufficient heat for melting ice and rapid evaporation. This study highlights the potential of T-MX-PAN fibers as next-generation wearable heaters and offers valuable insights into advancing wearable technology in demanding environments.

Multi‐step heating strategy during thermal stabilization of polyacrylonitrile multi‐filament bundle before carbonization

AbstractA polyacrylonitrile (PAN) multi‐filament bundle underwent a multi‐step heat treatment following impregnation with a solution of ammonium bromide, phosphoric acid and urea (A‐P‐U). The multi‐step thermal stabilization process was conducted in an air atmosphere at temperatures ranging from 200 to 245 °C for various stabilization times spanning from 10 to 70 min. Comprehensive analysis of physical and mechanical properties, infrared (IR) spectroscopy, X‐ray diffraction (XRD) and thermal analysis (DSC and TGA) revealed the significant influence of stabilization time on the structure and properties of thermally stabilized PAN multi‐filament bundle. The fiber thickness and linear density of the stabilized fibers decreased by approximately 29.5% and 10.6%, respectively, after 70 min of heat treatment. However, the fiber density value increased from 1.18 to 1.38 g cm−3 during the same stabilization time. Additionally, the carbon yield value obtained using TGA increased from 31% to 73% at 850 °C. The presence of A‐P‐U markedly reduced the time needed for the conversion of the PAN polymer into a cyclized structure through its nitrile groups, thereby accelerating the stabilization reactions. Furthermore, the IR spectra exhibited the appearance of CC bonds, signaling the creation of a crosslinked ladder‐like structure. The XRD traces confirmed the decrease in crystallinity with increasing stabilization time, consistent with the findings from IR spectra. The findings showed that the A‐P‐U integrated system is notably successful and proficient in promoting the cyclization of nitrile groups, thereby decreasing the time needed to establish a thermally stable structure capable of withstanding elevated carbonization temperatures. © 2024 The Author(s). Polymer International published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Vermicompost: Significance and Benefits for Agriculture

Vermicomposting is defined as the process of biodegradation and stabilization of organic materials, facilitated by the collaborative efforts of earthworms and mesophilic microorganisms. The activity of earthworms in vermicomposting results in the production of nutrient-rich vermicompost. This organic amendment is abundant in macro and micronutrients, vitamins, growth hormones, as well as enzymes like proteases, amylases, lipase, cellulase, and chitinase. Additionally, vermicompost harbors a diverse community of immobilized microflora. Even after being excreted by the worms, the enzymes in vermicompost persist in their ability to decompose organic matter, facilitating continued breakdown and nutrient release. The utilization of vermicompost in agricultural production brings forth a multitude of beneficial effects. These include decreased water requirements for irrigation, reduced susceptibility to pest and termite attacks, suppression of weed growth, and enhanced seed germination rates, accelerated growth and development of seedlings, as well as increased yield of fruits per plant in vegetable crops and a higher number of seeds per year in cereal crops. These advantages highlight the positive impact of incorporating vermicompost into agricultural practices, fostering sustainable and productive farming systems. The combination of earthworms and vermicompost presents a valuable opportunity to enhance horticultural production in a sustainable manner, reducing reliance on agrochemical inputs. Despite the numerous benefits associated with vermicompost, its widespread implementation and adoption are still relatively limited. This review aims to raise awareness and promote the understanding of this valuable local soil amendment.

Nutrient Composition of Detoxified Oil Cake from Simarouba glauca

Vermicomposting is defined as the process of biodegradation and stabilization of organic materials, facilitated by the collaborative efforts of earthworms and mesophilic microorganisms. The activity of earthworms in vermicomposting results in the production of nutrient-rich vermicompost. This organic amendment is abundant in macro and micronutrients, vitamins, growth hormones, as well as enzymes like proteases, amylases, lipase, cellulase, and chitinase. Additionally, vermicompost harbors a diverse community of immobilized microflora. Even after being excreted by the worms, the enzymes in vermicompost persist in their ability to decompose organic matter, facilitating continued breakdown and nutrient release. The utilization of vermicompost in agricultural production brings forth a multitude of beneficial effects. These include decreased water requirements for irrigation, reduced susceptibility to pest and termite attacks, suppression of weed growth, and enhanced seed germination rates, accelerated growth and development of seedlings, as well as increased yield of fruits per plant in vegetable crops and a higher number of seeds per year in cereal crops. These advantages highlight the positive impact of incorporating vermicompost into agricultural practices, fostering sustainable and productive farming systems. The combination of earthworms and vermicompost presents a valuable opportunity to enhance horticultural production in a sustainable manner, reducing reliance on agrochemical inputs. Despite the numerous benefits associated with vermicompost, its widespread implementation and adoption are still relatively limited. This review aims to raise awareness and promote the understanding of this valuable local soil amendment.

Post-Takeover Proficiency in Conditionally Automated Driving: Understanding Stabilization Time with Driving and Physiological Signals.

In the realm of conditionally automated driving, understanding the crucial transition phase after a takeover is paramount. This study delves into the concept of post-takeover stabilization by analyzing data recorded in two driving simulator experiments. By analyzing both driving and physiological signals, we investigate the time required for the driver to regain full control and adapt to the dynamic driving task following automation. Our findings show that the stabilization time varies between measured parameters. While the drivers achieved driving-related stabilization (winding, speed) in eight to ten seconds, physiological parameters (heart rate, phasic skin conductance) exhibited a prolonged response. By elucidating the temporal and cognitive dynamics underlying the stabilization process, our results pave the way for the development of more effective and user-friendly automated driving systems, ultimately enhancing safety and driving experience on the roads.

Major chromosome rearrangements in intergeneric wheat × rye hybrids in compatible and incompatible crosses detected by GBS read coverage analysis

The presence of incompatibility alleles in primary amphidiploids constitutes a reproductive barrier in newly synthesized wheat-rye hybrids. To overcome this barrier, the genome stabilization process includes large-scale chromosome rearrangements. In incompatible crosses resulting in fertile amphidiploids, the elimination of one of the incompatible alleles Eml-A1 or Eml-R1b can occur already in the somatic tissue of the wheat × rye hybrid embryo. We observed that the interaction of incompatible loci Eml-A1 of wheat and Eml-R1b of rye after overcoming embryo lethality leads to hybrid sterility in primary triticale. During subsequent seed reproductions (R1, R2 or R3) most of the chromosomes of A, B, D and R subgenomes undergo rearrangement or eliminations to increase the fertility of the amphidiploid by natural selection. Genotyping-by-sequencing (GBS) coverage analysis showed that improved fertility is associated with the elimination of entire and partial chromosomes carrying factors that either cause the disruption of plant development in hybrid plants or lead to the restoration of the euploid number of chromosomes (2n = 56) in the absence of one of the incompatible alleles. Highly fertile offspring obtained in compatible and incompatible crosses can be successfully adapted for the production of triticale pre-breeding stocks.

The Use of Spectroscopic Methods to Study Organic Matter in Virgin and Arable Soils: A Scoping Review

The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and geochemical conditions. The aim of this review is to identify and analyze studies related to the application of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy techniques to study the molecular composition and transformation of organic matter in virgin and arable soils. This article is mainly based on three research questions: (1) Which NMR spectroscopy techniques are used to study SOM, and what are their disadvantages and advantages? (2) How is the NMR spectroscopy technique used to study the molecular structure of different pools of SOM? (3) How is ESR spectroscopy used in SOM chemistry, and what are its advantages and limitations? Relevant studies published between 1996 and 2024 were searched in four databases: eLIBRARY, MDPI, ScienceDirect and Springer. We excluded non-English-language articles, review articles, non-peer-reviewed articles and other non-article publications, as well as publications that were not available according to the search protocols. Exclusion criteria for articles were studies that used NMR and EPR techniques to study non-SOM and where these techniques were not the primary methods. Our scoping review found that both solid-state and solution-state NMR spectroscopy are commonly used to study the structure of soil organic matter (SOM). Solution-phase NMR is particularly useful for studying soluble SOM components of a low molecular weight, whereas solid-phase NMR offers advantages such as higher 13C atom concentration for stronger signals and faster analysis time. However, solution-phase NMR has limitations including sample insolubility, potential signal aggregation and reduced sensitivity and resolution. Solid-state NMR is better at detecting non-protonated carbon atoms and identifying heterogeneous regions within structures. EPR spectroscopy, on the other hand, offers significant advantages in experimental biochemistry due to its high sensitivity and ability to provide detailed information about substances containing free radicals (FRs), aiding in the assessment of their reactivity and transformations. Understanding the FR structure in biopolymers can help to study the formation and transformation of SOM. The integration of two- and three-dimensional NMR spectroscopy with other analytical methods, such as chromatography, mass spectrometry, etc., provides a more comprehensive approach to deciphering the complex composition of SOM than one-dimensional techniques alone.