St Cross Research Articles

Ethnic inequities in routine childhood vaccinations in England 2006–2021: an observational cohort study using electronic health records

Population groups that are underserved by England's childhood vaccination programme must be identified to address the country's declining vaccination coverage. We examined routine childhood vaccination coverage in England by maternal ethnicity between 2006 and2021. We created first, second and fifth birthday cohorts using mother-child linked electronic health records from the Clinical Practice Research Datalink (CPRD) Aurum. After validation against the UK Health Security Agency (UKHSA) and National Health Service England (NHSE) annual statistical reports, we described vaccination coverage for each vaccine by ethnicity and year. We used modified Poisson regression to analyse the effect of ethnicity on receiving the primary and full course of each vaccine. Up to 1,170,804 children born after 1 April 2006 were included in the first birthday cohort, reducing to 645,492 by the fifth birthday. Children were followed up until 31 March 2021at the latest. Children born to mothers in 9 minority ethnic groups and those of unknown ethnicity had lower vaccination coverage (61.3-97.5%) than the White British group (79.9-97.8%) for all vaccines. Indian, Pakistani, Bangladeshi, Chinese, Any other Asian background, and White and Asian ethnic groups had similar vaccination coverage to the White British group (above 90% for most vaccines in most years). Inequities particularly affected the Caribbean group (e.g. 61% coverage for the 6/5/4-in-1 full course in 2020-21 by children's fifth birthday; RR 0.66, 95% CI 0.6-0.74 compared with the White British group) and Any other Black, African and Caribbean background (e.g. coverage 68% for the MMR primary course in 2020-21; RR 0.71, 95% CI 0.64-0.78). These inequities widened over the study period. For example, the absolute difference in coverage between the Caribbean and White British groups for the full course of MMR increased from 12% in 2011-12 to 22% in 2019-20. These inequities remained even after accounting for sociodemographic, maternal and birth related factors, and also widened from primary course to full course. Our findings suggest that urgent policy action is needed to address the ethnic inequities throughout England's routine childhood vaccination programme, which have been worsening over time. University of Oxford Clarendon Fund, St Cross College and Nuffield Department of Population Health.

From Cloudy Trophies to Quiet Power: Keats’s Hyperions, the 1819 Odes, and Michael O’Neill’s Late Poetry

This essay reads Keats’s 1819 poetry alongside Michael O’Neill’s poems about his terminal illness. It focuses on Keats’s abandonment of Hyperion and The Fall of Hyperion in favour of ‘To Autumn’ through a juxtaposed reading of ‘cloudy trophies’ and ‘quiet power’. The Hyperion poems explore the collapse of the Poetical Character in the face of incurable, immortal suffering. While the spring odes, exemplified in a reading of ‘Ode on Melancholy’, attempt to balance the dynamic between a speaker and its poetic subject through apostrophe, the conclusions of the odes are self-cancelling. ‘To Autumn’ signals a breakthrough from curative to palliative poetics through the simultaneous celebration of life insisting on itself and the process of dying. The contrast between Guy’s Hospital and the Hospital of St Cross in Winchester underlines the shift in vision from incurable suffering to palliative, quiet power.

Godfrey Harry Stafford. 15 April 1920—29 July 2013

Godfrey Harry Stafford's career as a physicist began with research in cosmic rays in the 1940s and he lived to see the discovery in 2012 of the Higgs boson at the CERN Large Hadron Collider. He made major contributions to the construction and exploitation of accelerators at the Rutherford Laboratory in the UK and was its director from 1969 to 1981. During this period he oversaw the diversification of the laboratory into the multi-disciplinary centre it is today. He was master of St Cross College, Oxford, from 1979 to 1987 and president of the Institute of Physics from 1986 to 1988. He was a major supporter of physics as an international activity: he was a founder member of the European Physical Society in 1968 and its president 1984–86, and he had significant links with CERN that spanned 25 years.

Effectiveness and Safety of Early Initiation of Poststernotomy Cardiac Rehabilitation Exercise Training

Guidelines recommend that cardiac rehabilitation (CR) exercise training should not start until 6 weeks after sternotomy, although this is not evidence based. Limited data suggest that starting earlier is not detrimental, but clinical trials are needed. To compare the effectiveness and safety of CR exercise training started either 2 weeks (early CR) or 6 weeks (usual-care CR) after sternotomy. This was an assessor-blind, noninferiority, parallel-group, randomized clinical trial that conducted participant recruitment from June 12, 2017, to March 17, 2020. Participants were consecutive cardiac surgery sternotomy patients recruited from 2 outpatient National Health Service rehabilitation centers: University Hospital, Coventry, UK, and Hospital of St Cross, Rugby, UK. Participants were randomly assigned to 8 weeks of twice-weekly supervised CR exercise training starting either 2 weeks (early CR) or 6 weeks (usual-care CR) after sternotomy. Exercise training adhered to existing guidelines, including functional strength and cardiovascular components. Outcomes were assessed at baseline (inpatient after surgery), after CR (10 or 14 weeks after sternotomy), and 12 months after randomization. The primary outcome was the change in 6-minute walk test distance from baseline to after CR. Secondary outcomes included safety, functional fitness, and quality of life. A total of 158 participants (mean [SD] age, 63 [11.5] years, 133 male patients [84.2%]) were randomly assigned to study groups; 118 patients (usual-care CR, 61 [51.7%]; early CR, 57 [48.3%]) were included in the primary analysis. Early CR was not inferior to usual-care CR (noninferiority margin, 35 m); the mean change in 6-minute walk distance from baseline to after CR was 28 m greater in the early CR group (95% CI, -11 to 66; P = .16). Mean differences for secondary outcomes were not statistically significant, indicating noninferiority of early CR. There were 46 vs 58 adverse events and 14 vs 18 serious adverse events in usual-care CR and early CR, respectively. There was no difference between the groups in the likelihood of participants having an adverse or serious adverse event. Starting exercise training from 2 weeks after sternotomy was as effective as starting 6 weeks after sternotomy for improving 6-minute walk distance. With appropriate precautions, clinicians and CR professionals can consider starting exercise training as early as 2 weeks after sternotomy. ClinicalTrials.gov Identifier: NCT03223558.

Mutagenic and genotoxic activities of Phospholipase A2 Bothropstoxin-I from Bothrops jararacussu in Drosophila melanogaster and human cell lines

Phospholipase A2 Bothropstoxin-I (PLA2 BthTX-I) is a myotoxic Lys49-PLA2 from Bothrops jararacussu snake venom. In order to evaluate the DNA damage caused by BthTX-I, we used the Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster and Comet assay in HUVEC and DU-145 cells. For SMART, different concentrations of BthTX-I (6.72 to 430 μg/mL) were used and no significant changes in the survival rate were observed. Significant frequency of mutant spots was observed for the ST cross at the highest concentration of BthTX-I due to recombinogenic activity. In the HB cross, BthTX-I increased the number of mutant spots at intermediate concentrations, being 53.75 μg/mL highly mutagenic and 107.5 μg/mL predominantly recombinogenic. The highest concentrations were neither mutagenic nor recombinogenic, which could indicate cytotoxicity in the wing cells of D. melanogaster. In vitro, all BthTX-I concentrations (1 to 50 μg/mL) induced decrease in HUVEC cell viability, as well as in DU-145 cells at concentrations of 10, 25, and 50 μg/mL. The comet assay showed that in HUVEC and DU-145 cells, all BthTX-I concentrations promoted increase of DNA damage. Further studies should be performed to elucidate the mechanism of action of PLA2 BthTX-I and its possible use in therapeutic strategies against cancer.

HERBICIDE GENOTOXICITY REVEALED WITH THE SOMATIC WING SPOT ASSAY OF Drosophila melanogaster

The genotoxicity of the herbicides clomazone, linuron and simazine was detected using the somatic wing spot assay of Drosophila melanogaster . For the evaluation of the mutagenic and recombinogenic activities induced by the herbicides two crosses were used: the standard cross (ST) which expresses basal levels of cytochrome P450 enzymes and the high bioactivation cross (HB) with overexpression of P450 genes conferring increased sensitivity to promutagens and procarcinogens. Third-instar larvae were exposed by chronic feeding (48 h) to three different concentrations of each herbicide. The frequencies of spots per individual in the treated series were compared to the negative concurrent control series (water or 5 % ethanol solution depending on the herbicide). Clomazone showed positive results for small and total spots in both the ST and the HB crosses. Linuron was positive at all concentrations tested for small and total spots in the ST cross while only positive for small and total spots at the highest concentration assayed in the HB cross. Simazine did not produce positive results in the ST cross while it was positive in the HB cross. The bifunctional cross-linking agent mitomycin C (MMC – 0.15 mM) was used as positive control and produced as expected a significant increase of all types of spots in both crosses. In conclusion, the positive results were due to induced mutations and not by recombinogenic activity, furthermore the significant increase in the frequency of total spots was mainly due to the induction of small single spots.

Assessment of mutagenic, recombinogenic and carcinogenic potential of titanium dioxide nanocristals in somatic cells of Drosophila melanogaster

Nanoparticles have been widely used in several sectors and their long-term effect on the body and environment remains unknown. To evaluate the mutagenic, recombinogenic and carcinogenic potential of 11 nm titanium dioxide nanocrystals (TiO2 NCs), the Somatic Mutation and Recombination Test (SMART) and the Test for Detection of Epithelial Tumors Clones (Warts-Wts) were used, both in Drosophila melanogaster. Third-instar larvae (72 + 4 h), obtained in both tests, were treated with different concentrations of TiO2 NCs ranging from 6.25 to 100 mM. Ultrapure water and urethane were used as negative and positive controls, respectively. At ST cross, all concentrations of TiO2 NCs showed a significant increase in the frequencies of mutant spots, demonstrating higher recombination rates. At the HB cross, only the 50 mM concentration showed a negative result. In the Wts Test, all used concentrations were carcinogenic, except for the 100 mM one, which was toxic. No relationship was demonstrated between the used concentrations and the obtained responses. There was no interference of the cytochrome P450 enzyme complex in the induction of mutant spots.

Evaluation of mutagenic, recombinogenic and carcinogenic potential of (+)-usnic acid in somatic cells of Drosophila melanogaster

The main of this study was to evaluate the mutagenic and carcinogenic potential of (+) – usnic acid (UA), using Somatic Mutation and Recombination Test (SMART) and the test for detecting epithelial tumor clones (wts) in Drosophila melanogaster. Larvae from 72 ± 4 h from Drosophila were fed with UA (5.0, 10.0 or 20.0 mM); urethane (10.0 mM) (positive control); and solvent (Milli-Q water, 1% Tween-80 and 3% ethanol) (negative control). ST cross produced increase in total mutant spots in the individuals treated with 5.0, 10.0 or 20.0 mM of UA. HB cross produced spot frequencies in the concentration of 5.0 mM that were higher than the frequency for the same concentration in the ST cross. In the highest concentrations the result was negative, which means that the difference observed can be attributed, in part, to the high levels of P450, suggesting that increasing the metabolic capacity maximized the toxic effect of these doses. In the evaluation of carcinogenesis using the wts test, the results obtained for the same concentrations of UA show a positive result for the presence of tumors when compared to the negative control. We conclude that UA has recombinogenic, mutagenic and carcinogenic effects on somatic cells in D. melanogaster.

Dedicated Followers of Fashion? Bioarchaeological Perspectives on Socio-Economic Status, Inequality, and Health in Urban Children from the Industrial Revolution (18th-19th C), England.

The 18th and 19th centuries in England were characterised by a period of increasing industrialisation of its urban centres. It was also one of widening social and health inequalities between the rich and the poor. Childhood is well‐documented as being a stage in the life course during which the body is particularly sensitive to adverse socio‐economic environments. This study therefore aims to examine the relationship between health and wealth through a comprehensive skeletal analysis of a sample of 403 children (0–17 years), of varying socio‐economic status, from four cemetery sites in London (c.1712–1854).Measurements of long bone diaphyseal length, cortical thickness, vertebral neural canal size, and the prevalence of a range of pathological indicators of health stress were recorded from the Chelsea Old Church (high status), St Benet Sherehog (middle status), Bow Baptist (middle status), and Cross Bones (low status) skeletal collections.Children from the low status Cross Bones site demonstrated deficient growth values, as expected. However, those from the high status site of Chelsea Old Church also demonstrated poor growth values during infancy. Fashionable child‐care practices (e.g. the use of artificial infant feeds and keeping children indoors) may have contributed to poor infant health amongst high status groups. However, differing health risks in the lower status group revealed the existence of substantial health inequality in London at this time. © 2016 The Authors International Journal of Osteoarchaeology Published by John Wiley & Sons Ltd.

Lack of mutagenic effect by multi-walled functionalized carbon nanotubes in the somatic cells of Drosophila melanogaster

Carbon nanotubes (CNTs) are formed by rolling up a single graphite sheet into a tube. Among the different types of CNTs, the multi-walled carbon nanotubes (MWCNTs) comprise a set of concentric nanotubes with perfect structures. Several uses for MWCNTs have been suggested to be included in biological applications such as manufacturing of biosensors, carriers of drugs. However, before these materials can be put on the market, it is necessary to know their genotoxic effects. Thus, this study aims to evaluate the mutagenicity of multi-walled carbon nanotubes (MWCNTs) functionalized in somatic cells of Drosophila melanogaster, using the somatic mutation and recombination test (SMART). This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the wings of the fly. Larvae of three days were used, resulting from ST cross, with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross). They were treated with different concentrations of MWCNTs functionalized. The MH descendants, analyzed in both ST and HB crosses, had no significant effects on the frequency of mutant. Based on the results and on the experimental conditions mentioned in this study, it was concluded that MWCNTs were not mutagenic in D. melanogaster.

Detection of transfluthrin and metofluthrin genotoxicity in the ST cross of the Drosophila Wing Spot Test

In this study, different concentrations of transfluthrin and metofluthrin have been assayed for genotoxicity by using the Wing Spot Test on Drosophila melanogaster. Standard cross was used in the experiment. Third-instar larvae that were trans-heterozygous for the two genetic markers mwh and flr3 were treated at different concentrations (0.0103mgmL−1, 0.103mgmL−1 for transfluthrin and 6μgmL−1, 60μgmL−1 for metofluthrin) of the test compounds. Feeding ended with pupation of the surviving larvae and the genetic changes induced in somatic cells of the wing’s imaginal discs lead to the formation of mutant clones on the wing blade. Results indicated that two experimental concentrations of transfluthrin and 60μgmL−1 metofluthrin showed mutagenic and recombinogenic effects in both the marker-heterozygous (MH) flies and the balancer-heterozygous (BH) flies.

Interactions of sulforaphane and dimethyl sulfoxide with methyl methanesulfonate, urethane, 4-nitroquinoline-1-oxide and hydrogen peroxide in the Drosophila melanogaster wing spot test

Sulforaphane (SF) is an isothiocyanate present in Brassicaceae, vegetables that induce the detoxification of electrophiles and reactive oxygen species. SF has been correlated with chemoprevention mechanisms against degenerative diseases. We tested if the SF had an effect against methyl methanesulfonate (MMS), urethane (URE), 4-NQO and H2O2. SF (>95% purity, 0.14, 0.28, 0.56mM) was diluted in a DMSO/Tw80/EtOH mixture (DTE) corresponding to 25, 50, 100% of lyophilized broccoli. The SF treatment (0.14mM) was positive for small spots in the ST cross and negative in the HB cross. In the HB cross, SF (0.28mM) was genotoxic. In the ST cross, the SF treatments showed a tendency to reduce the genotoxic damage caused by MMS, which could be explained by the radical scavenging action of the DTE mixture. In the ST cross, the frequency of small spots in the SF 0.14mM/URE treatment was similar to that of Water/URE, which can be explained by a DTE and SF scavenger action. In both crosses, the results for the direct oxidants, 4-NQO and H2O2, were different and must be related to differential modulation of CYPs expression and the SF and DTE scavenger properties.

Genotoxicity studies in the ST cross of the Drosophila wing spot test of sunflower and soybean oils before and after frying and boiling procedures

Sunflower and soybean oils were tested for genotoxicity in the Drosophila wing somatic mutation and recombination assay. Results indicate that both oils produce genotoxic effects when tested without any previous frying or boiling processes. Boiling sunflower oil during fifteen, thirty and sixty minutes significantly increased its genotoxic response; nevertheless, after frying potatoes this oil showed a significant decrease in the genotoxic activity. On the other hand, boiling and frying soybean oil in the same conditions results in a decrease of its genotoxic potential. We have also detected that the amount of total polar materials increases significantly in oils submitted to frying or boiling process. Nevertheless, in oils obtained after frying potatoes, the amount of TPM was higher than after boiling. It is suggested that this effect is probably due to the amount of non-volatile TPM, the fatty acid composition of the oils, the types of frying oil, the high frying temperature and time, and the number of boiling and frying. This is the first study reporting genotoxicity data in Drosophila for the boiling and frying of both sunflower and soybean oils.

John Dee: the patronage of a natural philosopher in Tudor England

For all of his failures to secure patronage, John Dee was successful compared with his contemporaries. We know more about his patronage relations than those of any other natural philosopher in Tudor England. Only by comparing him with other English client practitioners can we understand how unusual and even productive were Dee’s relations with his patrons. This article makes those comparisons and offers an overview of Dee’s patronage, but in the main it explores three of the unusual aspects.The first is Dee’s good relationship with female patrons and patronage brokers, notably Queen Elizabeth. The second is the kind of office that Dee sought. His greatest efforts were aimed at securing the headship of a collegiate institution such as Eton College or the Hospital of St Cross. Not only did they fit his aspiration to set up a research institute, but all were offices in the Queen’s direct gift, and so played to Dee’s strengths. Nevertheless, Dee was frustrated at nearly every turn. It is suggested that a major cause was the young Dee’s links with both Sir John Cheke’s network of Protestant humanists, who became Marian exiles, and with ‘Louvainist’ Catholic exiles opposed to Edward VI and Elizabeth. We see how Dee was consistently passed over in favour of other members of these circles. Notwithstanding this, it is concluded that Dee was relatively successful, given the English court’s refusal to patronise speculative natural philosophy, even that offered by Thomas Digges, William Gilbert or Francis Bacon. Finally, some of Dee’s supposed failings as a client, such as the sparseness of printed works, are shown to have been systemic.

John Dee’s ideas and plans for a national research institute

John Dee’s arrangements at his Mortlake house have received some attention as an English ‘academy’ or ‘experimental household.’ His ideas for St Cross, which he requested as a suitable living in 1592, have received less detailed attention. This paper examines Mortlake and his St Cross plans in detail and argues that, at their core, they shared an aspiration to create a national research institute. These plans are related to the context of Dee’s pursuit of royal patronage and his idea of the social and intellectual role of the natural philosopher. His ideas for a research institute are also placed in the context of alternatives to traditional sites of learning, including contemporary academies, princely courts, and independent establishments, indicating that Dee’s ideas, while part of a Renaissance vogue for such alternatives, were independent of other models. Discussion includes the historiographic debate regarding openness versus secrecy in the pursuit of natural knowledge. Finally, Dee’s ideas are contrasted with Francis Bacon’s proposal for a research institute, which also originated in the 1590s and culminated in ‘Solomon’s House.’

The Meat Crisis. Developing More Sustainable Production and Consumption Edited by J D’Silva and J Webster (2010). Published by Eartscan Ltd, 14a St Cross Street, London EC1N 8XA, UK. 328 pp Paperback (ISBN 978-1-84407-903-2). Price £24.99.

The Meat Crisis. Developing More Sustainable Production and Consumption Edited by J D’Silva and J Webster (2010). Published by Eartscan Ltd, 14a St Cross Street, London EC1N 8XA, UK. 328 pp Paperback (ISBN 978-1-84407-903-2). Price £24.99. - Volume 20 Issue 3

INFANT AND CHILD MORTALITY AMONG CATHOLICS AND LUTHERANS IN NINETEENTH CENTURY POZNAŃ

The purpose of this study was to show the differences in the mortality rates of children from Catholic and Lutheran families in 19th century Poznań, and to elucidate the causes of these differences. Data from Catholic and Lutheran parish death registers were used. The infant death rate (IDR), neonatal and postneonatal death rates and life table biometric functions were calculated and causes of deaths were characterized. The worst child mortality values (IDR=394.4; neonatal and postneonatal death rates, respectively, 117.1 and 277.4; e0=16.14 years; Crow's Index=2.47) were obtained for the poor Catholic Parish of St Margaret. The lowest infant and neonatal and postneonatal death rates were observed to have occurred in the Catholic Parish of St Maria Magdalena situated in the city's more affluent central area (mortality rates, respectively, 269.9, 93.1 and 176.9; e0=24.63 years; Crow's Index=0.96). The widest range of differences with regard to death rates was found for the Lutheran Parish of St Cross (the infant, neonatal and postneonatal death rates were, respectively, 293.1, 99.1 and 193.9; e0=28.03 years; Crow's Index=0.92). The St Cross Parish encompassed a fairly large area of the city characterized by varying ecological conditions. Among infants and young children from the three studied populations a high frequency of deaths due to infectious diseases, diarrhoeas, dysenteries and tuberculosis were observed. Differences in the mortality of children from Catholic and Lutheran families in 19th century Poznań resulted from ecological conditions, among which water played the most important role, rather than from religious differences.

The Diffusion of Early Franco-ItalianAll'AnticaOrnament: The Renaissance Frieze in the Chapel of the Hospital of St Cross, Winchester, and the Gaillon Stalls, Now at St Denis, Paris

In Volume 82 (2002) of theAntiquaries Journal,the canopied benches at St Cross were described, along with a discussion of their date and patronage. This paper takes the subject further by suggesting a reconstruction of the layout of these fittings. Conclusions reached in the earlier paper are further refined: the distinctive mouldings on the frieze, benches and desks clearly demonstrate that all three elements are integral to the same work and not added to preexisting benches, as previously argued. Further research suggests that the imagery and style of the frieze at St Cross had little impact on the aesthetic of the works patronized by Bishop Fox in Winchester Cathedral. Instead, parallels are drawn between the St Cross frieze and other modes ofall'anticawork in France, including the celebrated Renaissance stalls from the chapel of the archiepiscopal palace at the Château de Gaillon (Eure) and the cathedral stalls at Amiens (Somme). It has also become apparent that there is no other extant work in England that directly parallels the frieze at St Cross, and that the nearest analogue is the stallwork from Gaillon.

Mutagenic and recombinagenic activity of airborne particulates, PM10 and TSP, organic extracts in the Drosophila wing-spot test

The genotoxicity associated with air pollution in the city of Canoas, Rio Grande do Sul (Brazil), was assessed in November (spring) and January (summer). We applied the somatic mutation and recombination test (SMART) in Drosophila melanogaster in its standard version with normal bioactivation (ST) and in its variant with increased cytochrome P450-dependent biotransformation capacity (HB). The data indicated the genotoxicity of TSP and PM10 collected in November, in both ST and HB crosses. The genotoxic activity of the PM10 material in the spring sample was exclusively associated with the induction of mitotic recombination, whereas the TSP genetic toxicity was due to both recombinational as well as point and/or chromosomal mutation events. Considering PM10 collected in January, a positive response—100% (17.10 m 3/ml) concentration—was observed in the HB cross, which was not detected in the ST cross.

The history of science, medicine and technology at Oxford

The history of science came early to Oxford. Its first champion was Robert T. Gunther, the son of a keeper of zoology at the British Museum and a graduate of Magdalen College who took a first there in the School of Natural Science in 1892, specializing in zoology ([figure 1][1]). As tutor in natural