Bouguezzi, R, Negra, Y, Sammoud, S, Uthoff, A, Moran, J, Behrens, M, and Chaabene, H. The effects of volume-matched 1- and 2-day repeated backward sprint training formats on physical performance in youth male soccer players. J Strength Cond Res XX(X): 000-000, 2024-This study examined the effects of a 6-week, 1 vs. 2 sessions of volume-matched weekly repeated backward sprint training (RBST) on measures of physical fitness in youth male soccer players. Thirty male youth soccer players from a regional soccer team were randomly assigned to a 2-day group (n = 15; age = 16.40 ± 0.64 years; Maturity-offset = 2.19 ± 0.65 years) or a 1-day group (n = 15; age = 16.27 ± 0.51 years; Maturity-offset = 1.91 ± 0.40 years). Measures of jumping ability, linear sprint speed, change-of-direction (CoD) speed, aerobic endurance (AE), and repeated sprint ability (RSA) were measured before and after 6 weeks of training. The training interventions involved 1 to 2 sets, each comprising 7 repetitions over a 20-m distance of RBST in the 2-day group, and 2 to 4 sets, also with 7 repetitions each over the same distance, in the 1-day group. Statistical analyses were conducted using the analysis of covariance model with baseline measurements entered as covariates. Results indicated that RBST over 2 days generated greater benefits compared with a single day for improving CoD speed (∆4.91 vs. 0.04%; effect size [d] = 0.78 vs. 0.00, respectively), linear sprint speed (10-m: ∆4.74 vs. 0.36%; d = 0.96 vs. 0.00, respectively; 20 m: ∆3.34 vs. 0.82%; d = 1.00 vs. 0.49, respectively), and RSA performances (RSAbest: ∆2.61 vs. 0.29%; d = 0.71 vs. 0.16, respectively; RSAmean: ∆2.86 vs. 0.89%; d = 0.59 vs. 0.16, respectively; RSAtotal: ∆2.86 vs. 0.89%; d = 0.62 vs. 0.41, respectively). However, similar improvements in the 2 RBST formats were observed on jumping (2-day group: ∆6.9%; d = 0.75; 1-day group: ∆10.6%, d = 1.26) and AE performance (2-day group: ∆17.24%, d = 1.04; 1-day group: ∆27.25%, d = 2.25). In summary, the findings suggest that, when volume is matched, spreading the RBST regimen over 2 days may result in greater improvements compared with a single-day approach for enhancing CoD speed, linear sprint speed, and RSA performance.