A field studies were carried out in 2018-2020 in the Institute of seed production and agricultural technologies (a branch of the Federal state budgetary scientific institution "Federal scientific Agroengineering center VIM" (Ryazan region)) to study the productivity and environmental plasticity of multi-row barley. The object of research was multi-row barley varieties pallidum collection nursery and created on their basis promising lines from competitive variety testing. It was found that the Volgodon (Russia) and Helios (Ukraine) varieties are resistant to stress and have the ability to form stable productivity – 0.59 and – 1.34, respectively. From the studied lines, 35/4-14h 1171 and 27/2-11h787 – -1.37 and -1.40, respectively, were distinguished by this feature. The Herald (USA) had the lowest stress tolerance (- 3.57). The maximum average yield was shown by the L-2377 variety (Republic of Tatarstan) and lines 27/2-11h787, 35/1-14h 1171, 35/4-14h 1171 – more than 5.0 t/ha. The most productive varieties with high genetic flexibility are L-2377, Helios and lines 27/2-11h787, 35/1-14h 1171, 35/4-14h 1171. It was revealed that the L-2377 variety and the 27/2-11h787 line had an adaptability coefficient higher than 1 for 3 years of research. When analyzing the structure of the yield of multi-row barley, it was found that the greatest contribution to its value was made by two elements: the ear length (r=+0.52) and the mass of 1000 grains (r=+0.51). It was found that the following varieties have the greatest potential productivity, adaptability and stress resistance: L-2377, Helios, B-934 (Canada); and lines: 27/2-11h787, 35/1-14h 1171, 35/4-14h 1171. The selected multi-row varieties and promising lines of spring barley should be used in the selection process to further improve the created forms.