Twenty-five samples of spray-dried milk powder obtained from five dairy plants at various districts in Japan in May 1964, and six samples subjected to the previous work (the third report) were examined to clarify the flora of bacteria in them.The examination of bacterial flora were made by picking all colonies on two or three plates of Plate Count Agar for the colony counts.The predominating type of bacterial flora in samples from plant A and B was Bacillus. However, the type in samples from plant C as well as E, and in samples subjected to the previous work was enterococcus group, and in some cases it was enterococcus group only.