Background: The Oslo Sports Trauma Research Center (OSTRC) questionnaires on overuse injury and health problem were developed to register overuse injury and health problems. However, this questionnaire has not been translated or validated in Thai. Objective: To develop the original edition of the OSTRC Questionnaire on overuse injuries and health problems into Thai language and to examine the validity and reliability of the adapted scale. Materials and Methods: The development of the questionnaire followed the steps of translation, which included forward translation, translation merging, backward translation, and critique by the researcher, health professionals, speech professionals, athletes, and the translators for semantic and conceptual equivalence. A cohort of 65 Thai athletes were recruited. Cross-sectional surveillance data were used to record overuse injury and health problem. Throughout the 12-week surveillance period, all participants were assigned to complete the questionnaire within three days after receiving a questionnaire by e-mail. Robustness and reliability process was seen in the 57 athletes who completed their 12 weeks of surveillance period. Results: The OSTRC on overuse injuries and health problems Thai version (OSTRC-OT and HT) showed a high internal consistency. Cronbach’s α of the OSTRC-OT for ankle, knee, and hip regions was 0.919, 0.973, and 0.976, respectively, and the OSTRC-HT was 0.959 and an excellent test-retest reliability with intraclass correlation coefficient of the OSTRC-OT for ankle, knee, and hip regions at 0.994, 0.970, and 0.991, respectively, and the OSTRC-HT at 0.970; all p-values<0.001. Known-groups validity, the severity scores of the OSTRC-OT for ankle, knee, and hip regions, and the OSTRC-HT scores were statistically significant different between injury and non-injury groups. Conclusion: The validity and reliability of both questionnaires, the OSTRC-OT and the OSTRC-HT were at an excellent level. Moreover, the OSTRC-OT and OSTRC HT have an excellent ability to separate athletes who have an injury and health problem for those who do not. Keywords: Questionnaire, Sport, Overuse injury, Health problem