Oryzoborus Angolensis Research Articles

SEEDEATERS AND SEEDS AT A TECOMA SAVANNA IN THE SOUTHERN PANTANAL, BRAZIL

Seedeaters (Sporophila spp.) comprise a rich Neotropical bird group of seed consumers common in open habitats. In this study, we documented the feeding habits of seedeaters and seed production at a Tecoma savanna (dominated by Tabebuia aurea) in southern Pantanal, Brazil. We also analyzed the relationship between seed offer (abundance, richness, and diversity) and the number of seedeaters foraging across seasons. Six species (Sporophila angolensis, S. caerulescens, S. collaris S. hypoxantha, S.leucoptera, and S. lineola) occurred in the savanna, mainly in the height of the wet season, when seed production increased abruptly, attracting seedeaters. Seedeaters used 14 of 16 grass species that produced seeds. Indeed, the number of foraging seedeaters paralleled the abundance and diversity of seeds, and the number of species consumed. During much of the dry season, when seeds were not produced, the few remaining seedeaters mostly consumed arthropods and flowers. The diet of seedeaters ranged from mostly seeds (S. angolensis) to a moderate proportion of flowers and arthropods (S. leucoptera). The offer of a rich set of seeds attractive to seedeaters indicates that the Tecoma savanna is a seasonally important habitat for these birds. Of concern, large areas of native grasses in the Pantanal have recently been transformed into exotic pastures, and extensive fires have become common. Thus, conservation of this singular area is important for seedeaters, which move over wide areas searching for an abundant and diverse seed supply.

Management of captive Passeriformes in eastern Amazonia: the amateur breeder

The interactions between avifauna and the environment, including relationships with humans and their role in cultural practices, are part of ethnoornithological studies. The main objective of this study was to describe the ex situ management of passerines by amateur breeders for recreational and commercial purposes in a municipality in the Brazilian Amazon. Semi-structured interviews were conducted with 62 farmers who had 279 wild birds belonging to 23 species in captivity. These animals were kept in cages of different sizes. The birds’ diets were obtained from specialized commercial stores and offered daily to the animals according to age group and reproductive stage. The animals were kept in environments with good air flow, and the cages were cleaned, and the water was changed daily. Worming of birds was a routine practice, but infestations and some diseases were noted. Medications for treatment were obtained from local specialized stores. The reproductive management of passerine birds, especially Sporophila angolensis and Sporophila maximiliani, was performed by only three breeders. The results provide relevant empirical knowledge about the ex situ management of passerines, which is part of local cultural habits. However, this study indicates that there may be negative effects of maintaining these birds in captivity, such as the capture of free-ranging wild animals. Socio-educational measures are necessary to improve awareness of the sustainable use of environmental resources and encourage legalized breeding of passerines to help conserve the species. Keywords: wild animals, ethnozoology, ex situ management, birds, Sporophila.

Responses to environmental enrichment are associated with personality characteristics in chestnut-bellied seed finches (Sporophila angolensis)

Environmental enrichment (EE) is used to promote natural behaviours in captive animals and may hold promise as a form of pre-release training, a strategy for improving coping skills of translocated birds. We investigated the use of EE to enhance foraging and vigilance behaviours of captive Sporophila angolensis, which may be related to post-release survival. We also evaluated whether consistent individual behavioural differences affected birds’ responses to EE. We submitted 19 captive seed-finches to three short-term challenges: tonic immobility (TI), new environment (NE) and new object (NO) tests. TI behaviour is related to fear/escape response to potential predators and novelty tests (NE and NO) assess neophobia, which are ecologically relevant personality traits influencing the shyness-boldness continuum. We noted a pronounced variability among the individuals’ personality traits, both in their fear and escape-related responses in the TI test and along shy/bold z-scores in NE and NO tests. During a period of enrichment, birds spent more time foraging and less time in vigilance states compared with both control phases. Personality traits of the birds affected their responses to enrichment with bolder birds spending more time foraging. The EE-related decrease in vigilance was independent of the birds’ personality traits. Our findings highlight interactions between personality and rearing environment that may impact post-release outcomes for translocated animals.

Outbreak of paramyxovirus in Chestnut-bellied Seed-Finch (Sporophila angolensis). Transmission electron microscopy diagnosis / Surto de paramixovirose em curiós Sporophila angolensis). Diagnóstico por microscopia eletrônica de transmissão

The Chestnut-bellied Seed-Finch (Sporophila angolensis) is a bird constantly sought after as a cage bird, which is the main threat and the cause of its disappearance from the most inhabited regions of the country. Paramyxovirus, present in most breeding sites and Ecological Parks, is the disease that most affects birds with a high mortality rate, causing significant losses, both economic and to nature. Avian paramyxoviruses belong to the family Paramyxoviridae, which includes 21 serotypes (APMVs-1-21). Serotypes 2 and 3 are the ones that most infect passerines. Type 2 causes respiratory disorders, while the disease caused by serotype 3 is characterized by pancreatitis, conjunctivitis, vomiting, diarrhea, dyspnea, dysphagia, pancreatitis, CNS symptoms, and death. Free-ranging birds can act as viral reservoirs and spread the disease when subjected to stress factors. During the period of 2009, during the illegal commercialization of Brazilian birds, 218 Chestnut-bellied Seed-Finch were apprehended and sent to the CRAS (Wild Animals Recovery Center) of the Tietê Ecological Park. Of these, 112 arrived dead and the rest died during the year. About 35 birds were sent to the Electron Microscopy Laboratory of the Biological institute, for research on viral agents. During the necropsy, it was observed that the intestines were dilated, containing yellowish and watery stools. Small intestine fragments were processed by negative staining (rapid preparation), antigen immunolabeling with colloidal gold particles and resin embedding. Under the transmission electron microscope, using the negative staining technique, pleomorphic, rounded or elongated, enveloped paramyxovirus particles containing helical herring-bone like nucleocapsid, measuring between 100 and 500 nm in diameter, were visualized in the samples of all birds. The antigen-antibody reaction was enhanced by colloidal gold particles. Ultrathin sections of the small intestine revealed the presence of nuclei with marginalized chromatin, containing intranuclear inclusions; amorphous intracytoplasmic inclusions, formed by helical nucleocapsids, complete particles measuring from 100 to 250 nm and incomplete ones, budding from the plasma membrane.

The use of Passeriformes in the eastern Amazonia of Brazil: culture encourages hunting and profit encourages trade

AbstractThis study characterized the ethnoornithological knowledge of passerine bird keepers, known locally ascriadoresorpassarinheiros, who capture, train, breed and sell wild species in the eastern Amazonia of Brazil. The data were collected from 62 bird keepers through informal, semi-structured interviews and participant observation. The family Thraupidae was the most captured group, with 23 species used, predominantly of the genusSporophila.Sporophila angolensishad the highest use value and price. Our findings suggest that conservation strategies should be a priority forSporophila maximiliani,S. angolensesandSaltator maximus. The Passeriformes are obtained by capturing them from the wild, by captive breeding or in trade. They are trapped and sold locally, in nearby municipalities, and also in more distant locations as far as the border with Suriname, French Guiana and Guyana. On the international market, sale prices can reach USD 5,400 per individual bird. Species with both significant economic value and regional cultural significance are of the greatest importance to the bird keepers. To mitigate the negative effects of this activity, we recommend implementation of environmental education programmes for bird keepers, to improve their capacity to raise awareness about wildlife, and to inform them of the legal processes for bird keeping and for breeding birds in captivity for legal trade. Long-term strategies for bird conservation should also be considered, such as the implementation of ecotourism in protected areas to encourage the practice of birdwatching.

Aves silvestres criadas em cativeiro na cidade de Eirunepé, médio rio Juruá, sudoeste da Amazônia brasileira

Neste estudo, realizamos um levantamento de aves silvestres em cativeiro e o modo de manutenção (criação) desses animais na cidade de Eirunepé, Amazonas, Brasil. Coletamos os dados de duas formas: sorteio de ruas a serem amostradas e indicação de criadores por informantes. Registramos 142 aves, pertencentes a 15 espécies de Psittacidae, Rallidae e Thraupidae. As três espécies com os maiores percentuais de registro foram Sporophila angolensis (34,5%, n = 49), S. lineola (33,8%, n = 48) e Amazona festiva (9,2%, n = 13). Os modos de criação registrados foram: gaiolas (84,51%, n = 120), soltas nas imediações da residência (9,15%, n = 13) e amarradas pelo tarso (6,34%, n = 9). Das espécies registradas, apenas S. cf. maximiliani está criticamente ameaçada de extinção. Concluímos que a manutenção de aves cativas em Eirunepé está, em grande parte, concentrada em passeriformes e que as finalidades da criação podem ser para estimação, uso em disputas de canto e rinhas ou reabilitação e, além disso, a manutenção das aves pode variar de acordo com o tamanho dos indivíduos. É fundamental que os criadores tenham conhecimento sobre as maneiras adequadas de criação e de como obter aves silvestres de maneira legal no Brasil.

Non-invasive stress monitoring and temperament of chestnut-bellied seed-finch (Passeriformes, Thraupidae)

Songbirds are kept as pets around the world, and bird-singing contests occur in several countries. However, there is a lack of scientific support for songbird welfare and stress monitoring. Thus, we aimed to validate the measurement of glucocorticoid metabolites (GCM) concentrations in bird droppings as a way to assess endogenous adrenal activity in the chestnut-bellied seed-finch (Sporophila angolensis) by using an adrenocorticotropic hormone (ACTH) challenge. We also aimed to describe behaviors potentially indicative of stress and to evaluate the role of temperament in the expression of stress in this species. For the ACTH challenge, 12 seed-finch adult males were randomly submitted to one of the three treatments: S – 100 μL saline solution (control); D1 – 0.5 μg of ACTH; and D2 – 1.0 μg of ACTH. Total bird droppings were collected during pre-challenge and challenge days at intervals of four and two hours, respectively. We also quantified the total time birds spent on the following behavioral states: alertness, freezing, comfort, flying and foraging during the pre-challenge, challenge and post-challenge days. The birds’ temperament was assessed during three challenge tests involving novelty. The mean baseline GCM concentration, determined on the pre-challenge day, was 68.7 ± 20.2 ng/g of dry bird droppings. Birds that received treatments D1 and D2 showed an increase of 2.2 times in the GCM concentration 12 h after treatment injections. The chestnut-bellied seed-finch showed individual behavioral distinctiveness, which was correlated with the time they spent on foraging in response to acute stress. Our study showed that GCM concentration reflects endogenous adrenal activity in the chestnut-bellied seed-finch. However, this measure alone is not enough as an indicator of songbirds’ welfare. Therefore, the valences (positive or negative) of freezing, alertness, flying, and foraging behaviors can be used together with the physiological measure for noninvasive stress monitoring in S. angolensis.

Polymorphic microsatellite loci and partial mitogenome for the Chestnut-bellied Seed-finch Sporophila angolensis (Aves, Passeriformes) using next generation sequencing.

Brazil is one of the major contributors to international trade in wildlife and species of the bird genus Sporophila are currently under threat due to illegal trade. Microsatellite loci and mitochondrial DNA constitute important molecular markers for population genetics studies and parentage analyses, and hold great potential to help authorities manage illegal trafficking and control commercial breeders. We describe and characterize 19 polymorphic microsatellite loci and recover part of the mitochondrial genome for Sporophila angolensis using massive parallel sequencing with the Illumina platform. DNA sequencing resulted in a dataset with 2,379,295 paired reads, of which 392 were mapped to the mitogenome of S. maximiliani, resulting in a partial mitogenome of 16,785bp for S. angolensis. The microsatellite search identified a total of 4737 loci, from which 27 primer pairs were tested on 24 individuals of unknown geographic origin. Nineteen of the 27 loci were successfully amplified and exhibited high levels of genetic variation, with a mean of 11.2 alleles per locus, a mean observed heterozygosity of 0.588 and a mean expected heterozygosity of 0.852. About half of the loci showed significant evidence for the presence of a null allele and significant deviation from Hardy-Weinberg equilibrium; the remaining eight loci had high paternity exclusion probabilities and low identity probabilities. The high levels of polymorphism for these loci, as well as their high paternity exclusion probability and low identity probability, indicate that they hold potential for parentage analyses and population genetics studies of S. angolensis.

Trends in Illegal Trade of Wild Birds in Amazonas State, Brazil

This study evaluates the seizure of birds in the state of Amazonas during twenty years (1992-2011), providing information on the sale of birds in a state recognized for its significant biodiversity. We compiled a total of 2,698 seizure records of illegal wildlife trade, which were drawn up and issued by the Brazilian Institute of Environment and Renewable Natural Resources - IBAMA (the Brazilian official agency for environmental license and patrol) in the state of Amazonas, during 240 months. Reports of birds seized were found in only 297 (11%) of the seizure records analyzed. The number of bird specimens ranged from 3 to 710 per year, and the taxonomic richness ranged from 1 to 24 species. Considering all seizures, there was a richness of 40 bird species, distributed in 16 families and 10 orders. Among the families analyzed, Thraupidae was the richest, with 17 species, followed by Psittacidae, with eight species. The order Passeriformes was the most represented, with 12 genera, where Sporophila was the richest, with nine species (22.5% of total species). The Saffron Finch ( Sicalis flaveola – Linnaeus, 1766), Muscovy Duck ( Cairina moschata - Linnaeus, 1758) and Chestnut-bellied Seed-Finch ( Sporophila angolensis - Linnaeus, 1766) together accounted for more than half (56.9%) of the total birds seized for the period. Of the species recorded in this study, five (12.5%) were listed as Endangered. Our results suggest that the illegal trade of animals in Amazonas shows a pattern different from that which has been reported in other Brazilian states. Birds were little represented in the Amazon, and the main reason for their illegal trade was related to their use as food. In addition to the cultural aspects, the richness of vertebrates in the Amazon certainly influences the choice of animals used and marketed in the Amazon region.

Reference values for the production of the aqueous fraction of the tear film measured by the standardized endodontic absorbent paper point test in different exotic and laboratory animal species

The aqueous fraction of the tear film and the horizontal palpebral fissure length (HPFL) were measured in exotic and laboratory animals, specifically saffron finches (Sicalis flaveola), chestnut-bellied seed-finches (Sporophila angolensis), red-eared sliders (Trachemys scripta elegans), rats (Rattus norvegicus) and mice (Mus musculus). These species possess small eyes making it difficult to perform the typical Schirmer tear test. Measurement of the aqueous fraction of the tear was performed using the standardized endodontic absorbent paper point tear test (PPTT), accomplished with manual restraint by a single operator. The following results were obtained: saffron finches (n = 42)-HPFL (4.46 ± 0.09 mm) and PPTT (5.10 ± 0.26 mm); chestnut-bellied seed-finches (n = 38)-HPFL (4.77 ± 0.05 mm) and PPTT (4.11 ± 0.34 mm); red-eared sliders (n = 56)-HPFL (8.59 ± 0.08 mm) and PPTT (8.79 ± 0.38 mm); rats (n = 60)-HPFL (6.45 ± 0.09 mm) and PTT (6.18 ± 2.06 mm); and mice (n = 22)-HPFL (3.59 ± 0.27 mm) and PPTT (4.39 ± 1.45 mm).

Predation on artificial nests in open habitats of central Brazil: effects of time and egg size

The accuracy of artificial nests in representing natural patterns of nest predation has been widely studied in temperate regions and egg size is one of the most tested sources of bias. In the neotropics, experiments with artificial nests usually used larger than natural eggs, despite suggestions in literature that the eggs should be similar to those of the local species. Here, we tested the hypothesis of spatial-temporal variation in predation risk of artificial nests in relation to egg size. We used quail (Coturnix coturnix; large), Chestnut-bellied Seed-finch (Oryzoborus angolensis; small) and plasticine (both sizes) eggs placed in artificial nests. We analyzed daily nest survival using models in the Program MARK. The best-fit model included the effects of egg size and the reproductive period on daily survival of artificial nests. Nests with large eggs had greater Daily Survival Estimates (DSE) than nests with small eggs during two times and DSE were smaller early in the reproductive period. DSE of 0.82 (95% CI = 0.76 to 0.86) and 0.91 (0.87 to 0.93) were recorded for large eggs at the beginning and end periods compared to 0.72 (0.65 to 0.78) and 0.84 (0.79 to 0.88) for small eggs. Birds were more important predators (40% of records) than mammals and reptiles based on signs left on the plasticine eggs. Our results support the hypothesis that larger eggs are less depredated than small ones in artificial bird nests, even when main predators (birds) are different from those commonly observed in the Neotropics (mammals and reptiles). However, controlling the egg size in experiments with artificial nests may not be sufficient to obtain similar time patterns observed in natural nests studied in the Cerrado.

POST-MORTEM LESIONS OF UROLITHIASIS IN A LESSER SEED FINCH (Sporophila angolensis)

Urolithiasis is a disease that despite being a commonly observed problem in veterinary practice is uncommon in birds. Such disease was not reported in passeriforms to date. Accordingly, the aim of the present article is to describe a case of urolithiasis in an adult female lesser seed finch (Sporophila angolensis) pet bird which presented abdominal distension, respiratory distress, and apathy prior to death. The bird had history of being fed with a diet rich in protein. After the bird death, a necropsy was conducted in order to determine the cause of death. At necropsy, accentuated ascites, hydropericardium, and ureteral stones in the left ureter could be grossly observed. Additional tests related with viral and bacterial microbiological testing and with the determination of calculi composition could not be performed since the owner did not consent with the procedures because of the cost. Since the bird was fed on a high protein diet, a relationship between the ureteroliths and dietary imbalance was suggested with participation of protein in calculi development by providing the organic nuclei. Additionally, we conclude that the presence of calculi in the ureter resulted in urinary flow blockage, ascites, and consequent acute respiratory failure due to filling of air sacs with liquid.

Physical, epidemiological, and molecular evaluation of infection by Cryptosporidium galli in Passeriformes

Due to the scarcity of information related to the epidemiology of Cryptosporidium infection in passerine birds, this study aimed to determine the periodicity of fecal shedding of Cryptosporidium spp. oocysts, after natural infection, and its clinical signs, mortality, and molecular characterization. Four hundred eighty fecal samples were collected from 40 birds, including 372 samples from 31 adult birds and 108 samples from nine young birds (up to 12 months old), housed in five aviaries, monthly from September 2007 to September 2008, with the exception of April. The birds originated from aviaries in which the following species were raised: great-billed seed-finch (Oryzoborus maximiliani), lesser seed-finch (Oryzoborus angolensis), ultramarine grosbeak (Cyanocompsa brissonii), and rusty-collared seedeater (Sporophila collaris). The samples were preserved in 2.5% potassium dichromate at 4 degrees C until processing. The oocysts were purified by centrifugal flotation in Sheather's solution, followed by genomic DNA extraction and molecular characterization of oocysts using the nested polymerase chain reaction for amplification of fragments of the 18S subunit of rRNA gene. Intermittent shedding of oocysts was observed by positive amplification for Cryptosporidium spp. in 91 (24.5%) samples of adult birds and 14 (13%) of young birds. The sequencing of the amplified fragments enabled the identification of Cryptosporidium galli. Although all the aviaries had birds positive for C. galli, morbidity or mortality was observed in only one aviary and was associated with concomitant infection with Escherichia coli and Isospora sp.

Natural Infection with Cryptosporidium galli in Canaries (Serinus canaria), in a Cockatiel (Nymphicus hollandicus), and in Lesser Seed-Finches (Oryzoborus angolensis) from Brazil

Proventricular infection by Cryptosporidium sp. or Cryptosporidium galli has been associated with mortality, weight loss, diarrhea, and pasty feces. The purpose of this study is to report the occurrence of natural C galli infection in canaries (Serinus canaria), in a cockatiel (Nymphicus hollandicus), and in lesser seed-finches (Oryzoborus angolensis) with clinical complaints of apathy and sporadic mortality. Screening for Cryptosporidium spp. using microscopic examination of fecal samples and stained smears, histopathology, and nested polymerase chain reaction for actin gene and 18S ribosomal RNA gene following sequencing of amplified fragments allowed for the identification of C galli. To the best of our knowledge, this is the first report of C galli in birds in Brazil and the first report of this species in lesser seed-finches.

Intestinal protozoan parasites with zoonotic potential in birds

The aim of this study was to evaluate the occurrence of potentially zoonotic intestinal protozoan infections in exotic and wildlife Brazilian birds. Fecal samples from 207 birds of 45 species were examined. Infections by Balantidium sp., Entamoeba sp., and Blastocystis sp. were observed in 17 individuals (8.2%) of Gnorimopsar chopi, Oryzoborus angolensis, Sporophila caerulescens, Ramphastos toco, Aratinga leucophtalmus, and Pavo cristatus.

A new subspecies of Sporophila angolensis (Aves: Emberizidae) from Isla de Coiba and other additions to the avifauna of the Veragua Archipelago, Panama

New information is presented concerning the systematics and biogeography of the islands of the Pacific coast of western Panama. Sporophila angolensis fractor, new subspecies, characterized by a larger bill than S. a. ochrogyne, is described from Isla de Coiba, Panama. Recent doubt cast on the validity of the Coiba endemic subspecies Polioptila plumbea cinericia is unfounded. The first specimen records are given for Islas Secas and Isla Jicarón, including a specimen of Contopus cinereus from the latter that is referable to the Coiba subspecies C. c. aithalodes. Tyrannus melancholicus and Elaenia flavogaster are added to the avifauna of Isla Brincanco.

Three new species of Isospora Schneider, 1881 (Apicomplexa: Eimeriidae) from the lesser seed-finch, Oryzoborus angolensis (Passeriformes: Emberizidae) from Brazil

Three new coccidian (Apicomplexa: Eimeriidae) species are reported from the lesser seed-finch, Oryzoborus angolensis from Brazil. Sporulated oocysts of Isospora curio n. sp. are spherical to subspherical; 24.6 x 23.6 (22-26 x 22-25) microm, shape-index (SI, length/width) of 1.04 (1.00-1.15). Oocyst wall is bilayerd, approximately 1.5 microm thick, smooth and colourless. Micropyle and oocyst residuum are absent. The sporocysts are ovoid, 13.2 x 10.9 (15-17 x 10-13) microm, SI = 1.56 (1.42-1.71), with a small Stieda body and residuum composed of numerous granules scattered among the sporozoites. Sporozoites are elongated and posses a smooth surface and two distinct refractile bodies. Oocysts of Isospora braziliensis n. sp. are spherical to subspherical, 17.8 x 16.9 (16-19 x 16-18) microm, with a shape-index of 1.06 (1.00-1.12) and a smooth, single-layered wall approximately 1 microm thick. A micropyle, oocyst residuum and polar granules are absent. Sporocysts are ellipsoid and slightly asymmetric, 13.2 x 10.8 (12-14 x 9-12) microm, SI = 1.48 (1.34-1.61). Each sporocyst contains a barely visible Stieda body and a residuum composed numerous of granules. Sporozoites are elongated and each of them contains two distinct refractile bodies. Oocysts of Isospora paranaensis n. sp. are subspherical to broadly ellipsoid 24.3 x 19.8 (22-26 x 18-22) microm, SI = 1.22 (1.15-1.38) with smooth single-layered wall approximately 1.5 microm thick. A micropyle and oocyst residuum are absent, but one distinct ellipsoid polar granule (2.5-3.5 x 1.5-2.5 microm) is present. Sporocyst are ovoid, 15.7 x 10.1 (14-18 x 8-12) microm, SI = 1.46 (1.31-1.72), with distinct Stieda and sub-Stieda bodies. Each sporocyst contains a spherical sporocyst residuum, 4 microm in diameter All described isosporan species represent a possible cause of acute coccidiosis for O. angolensis in captivity.

Pequena contribuição à história natural de alguns Fringillidae do Brasil (Passeriformes)

A short contribution to the Natural History of some Brazilian Frigillidae The following species of Brazilian Fringillidae are mentioned here, the first of which being more deeply studied: 1 - Oryzoborus angolensis angolensis (Linnaeus). 2 - Oryzoborus crassirostris maximiliani Cabanis. 3 - Cyanocompsa cyanea sterea Oberholser. 4 - Coryphospingus cucullatus rubescens (Swainson). About each one of the referred species, the Author gives native names, some datas and observations on its reproduction and behaviour under captivity, as well as on its natural alimentation. Some considerations about the geographical races of Oryzoborus angolensis: O. a. angolensis (Linnaeus) and 0. a. torridus (Scopoli) -are also made. Both the races occur in Brasil and, according to the Author's opinion, they are not satisfactorily caracterized.