A microwave Mach–Zehnder Interferometer (MZI) sensor for the permittivity characterization of liquids is presented in this paper. Two spoof surface plasmon polariton (SSPP) waveguide transmission lines (TLs) composed of compact spiral units is utilized in a differential architecture. Loading a dielectric material on the sensing arm induces a phase difference between the two arms, leading to periodic interference dips on the transmission curve. The spectral positions and magnitudes of these dips change with different dielectric materials. Due to the nonlinear dispersion characteristics of the SSPP TL, the proposed SSPP-based MZI sensor achieves improved sensitivity compared with microstrip-based MZI sensor. To validate the sensor’s performance, six different oil samples and a set of adulterated oils were measured, incorporating a polydimethylsiloxane (PDMS) channel on the sensing arm. The variations in the spectral position and magnitude of the interference dip centered at 3.2 GHz are employed to determine the dielectric constants and loss tangents of the tested oils. The sensitivity in measuring the dielectric constant is 3.61 %. The maximum errors for measuring the dielectric constant and the loss tangent are 4.3 % and 6.7 %, respectively. Compared to other sensors for liquid oil detection, the proposed sensor provides a relatively high sensitivity while maintaining a low liquid volume, making it a promising candidate for permittivity measurements of liquid analytes and solid materials with similar dielectric constants.