The present study evaluated the anxiolytic effect of the herbal remedy “Ky cuc dia hoang thang gia vi” in experimental animals. Swiss mice were treated with “Ky cuc dia hoang thang gia vi” remedy at 31.2 and 62.4 g/kg per day for seven consecutive days. The anxiolytic effect of “Ky cuc dia hoang thang gia vi” was assessed in mice using elevated plus maze, spontaneous locomotor activity, rotarod performance and grip strength tests. The results showed that “Ky cuc dia hoang thang gia vi” remedy increased the frequency and time in the open arms and decreased the index of open arm avoidance. Moreover, “Ky cuc dia hoang thang gia vi” remedy inhibited spontaneous locomotion, shortened the stay time on the rotating cylinder in rotarod performance test, and reduced the force in the grip strength test. No statistically significant difference in the anxiolytic effect was found between two doses after three hours of the last administration. Our findings demonstrated that “Ky cuc dia hoang thang gia vi” remedy exerted anxiolytic effect. The rationale for the ethnomedicinal use of “Ky cuc dia hoang thang gia vi” remedy for the management of anxiety was confirmed scientifically in this study.