To date, traditional resistance training (TRT) programs incorporate a minute amount of instability training (IT). Over several decades TRT has transformed and taken on new and unusual concepts, such as Total Body Resistance Exercise (TRX). However, very little research has been completed investigating the effects of the TRX. PURPOSE: Therefore, the purpose of the study was to measure electrical muscle activity via electromyography (EMG) while performing a bodyweight split-squat in a stable environment compared to an unstable environment (i.e, TRX). METHODS: Twenty non-athlete (10 male; 10 female) experienced resistance and/or aerobically trained individuals participated in the investigation. The study consisted of three sessions including two variations of bodyweight split-squats and a training session to acclimatize the participants. The TRX split-squat was performed by placing the rear foot within the foot cradle of the TRX strap, while the traditional split-squat required the participant to place the rear foot on a stable bench, both 16 inches in height. Each session was separated by one minute of rest and each split-squat required the participant to perform three correct, consecutive, repetitions. EMG analysis was performed to assess the muscle activity of the gluteus maximus (GMa) and rectus femoris (RF). Final EMG data for the GMa and RF was analyzed via paired samples t-tests using IBM SPSS v. 23, between gender and environment. RESULTS: Paired samples t-tests were performed to compare the relative amount of EMG activity of the RF and GMa, between the TRX and traditional split squat. Significantly (p<0.001) higher EMG activity was displayed for the GMa during the TRX split-squat (569.91 ± 138.47 mV), compared to the traditional split-squat (499.78 ± 119.34 mV). No other significant differences in EMG activity of the GMa or RF were observed. CONCLUSIONS: The results were significant for the EMG activity of the GMa during the TRX split squat, which most likely was due to stabilizing any medial or lateral rotation of the trail leg knee. Little research has utilized the TRX and this investigation provides insight for future studies. Future implications include, perhaps involving an external resistance or performing another movement with the TRX, such as a two-leg squat or reverse lunge, may elicit greater results.