Splinted Model Research Articles

A new activity coefficient model for describing adsorbed phase nonidealities

The following study introduces a new adsorbed phase activity coefficient model, the SPLINT model, for describing nonideal multicomponent systems. The derivation is presented and simplification to the thermodynamically robust ABC model in the low‑coverage limit is shown. Both the SPLINT and ABC models are fit to 25 binary systems. Thermodynamic consistency of the data is established through multiple consistency tests including the intersection rule, closed‑loop reduced spreading pressure integration, the Gibbs‑Duhem integral equality, and alignment of pure and binary data. Regressed parameters are used in Real Adsorbed Solution Theory to predict both binary and ternary adsorption. The ABC and SPLINT models accurately describe systems with considerable nonideality when the activity coefficients are symmetric; however, the SPLINT model is required for accurate description of systems with complex, asymmetric activity coefficient behavior. The new model includes one more fitting parameter than the ABC model for a single binary system, but less across multiple data sets and higher‑order systems due to the theoretical basis. The importance of capturing activity coefficient asymmetry is demonstrated with a highly nonideal system across various pressures and temperatures measured by the present authors using the Integral Mass Balance method with HFC‑125 (pentafluoroethane), HFC‑32 (difluoromethane), and zeolite H‑ZSM‑5. The theoretical basis of the SPLINT model is further enforced by presenting two methods for predicting activity coefficients with the SPLINT model: correlating the fitting parameters individually and simultaneously regressing pure isosteric heat data to extract SPLINT fitting parameters. The study concludes with a discussion of pure and binary HFC‑125/HFC‑32 data with zeolite H‑ZSM‑5. A possible binary adsorption mechanism is proposed based on isosteric heat and excess enthalpy predictions and observed system irreversibility.

Comparative Analysis of Antibacterial and Wound Healing Activities of Chitosan and Povidone-Iodine-Based Hydrogels.

This study aimed to compare the antibacterial and wound healing efficacies of chitosan hydrogel with povidone-iodine (PI) hydrogel. The in vitro antibacterial activities of chitosan and PI hydrogels against Staphylococcus aureus and Escherichia coli were evaluated. Nine 6- to 8-week-old male Sprague-Dawley rats were divided into plain, PI, and chitosan hydrogel groups. Each rat received two 10-mm full-thickness dorsal wounds using an excisional splinting model. Each wound was treated with 0.2 mL of gel thrice over the course of 3 postoperative weeks. Weekly observations were conducted, and at the end of the third postoperative week, the rats were killed for histopathological and quantitative polymerase chain reaction evaluations. Data analysis included both 2- and 1-way analyses of variance. Chitosan hydrogel exhibited comparable in vitro antibacterial activity and a significantly enhanced in vivo wound closure rate compared with PI hydrogel. Three weeks after the surgery, the chitosan hydrogel group demonstrated marked differences in wound repair (P < 0.01). Histologically, increased collagen deposition was observed with chitosan hydrogel treatment. Immunohistochemistry for CD68 revealed a lower number of macrophages in the wounds treated with chitosan hydrogel. Quantitative polymerase chain reaction analysis indicated a superior collagen 1 to 3 ratio and reduced expression of proinflammatory cytokine mRNAs (interleukin 1b, interleukin 6, tumor necrosis factor α, and interferon γ) in the chitosan hydrogel group. Chitosan hydrogel demonstrates the potential to serve as an effective alternative to PI hydrogel, providing enhanced wound healing capabilities while maintaining comparable antimicrobial properties.

Two-Dimensional Biodegradable Black Phosphorus Nanosheets Promote Large Full-Thickness Wound Healing through In Situ Regeneration Therapy.

Large full-thickness skin lesions have been one of the most challenging clinical problems in plastic surgery repair and reconstruction. To achieve in situ skin regeneration and perfect clinical outcomes, we must address two significant obstacles: angiogenesis deficiency and inflammatory dysfunction. Recently, black phosphorus has shown great promise in wound healing. However, few studies have explored the bio-effects of BP to promote in situ skin regeneration based on its nanoproperties. Here, to investigate whether black phosphorus nanosheets have positive bio-effects on in situ skin repair, we verified black phosphorus nanosheets' positive effects on angiogenic and anti-inflammatory abilities in vitro. Next, the in vivo evaluation performed on the rat large full-thickness excisional wound splinting model more comprehensively showed that the positive bio-effects of black phosphorus nanosheets are multilevel in wound healing, which can effectively enhance anti-inflammatory ability, angiogenesis, collagen deposition, and skin re-epithelialization. Then, multiomics analysis was performed to explore further the mechanism of black phosphorus nanosheets' regulation of endothelial cells in depth. Molecular mechanistically, black phosphorus nanosheets activated the JAK-STAT-OAS signaling pathway to promote cellular function and mitochondrial energy metabolism in endothelial cells. This study can provide a theoretical basis for applying two-dimensional black phosphorus nanosheets as nanomedicine to achieve in situ tissue regeneration in complex human pathological microenvironments, guiding the subsequent optimization of black phosphorus.

Adipose Derived Stem Cell‐Conditioned Media As Alternative of Stem Cell Therapy for Diabetic Wound Healing

Diabetes mellitus (DM) is a set of metabolic disorder primarily characterized by chronic hyperglycaemia state, which is linked with several complications including retinopathy, neuropathy, and delayed wound healing. Adipose derived stem cells conditioned media (ADSCs-CM) contains several cytokines, and growth factors that can be used for treatment of diabetes-associated wounds. This study was conducted to explore the therapeutic potential of ADSCs- CM in excisional wound model in diabetic mice. Diabetes was induced in mice through Streptozotocin (STZ). Then, excisional wound splinting model in diabetic mice was developed. Subcutaneous injections of ADSC-CM (pure) and ADSC-CM conjugated with zinc sulphate were applied on the mice wound bed. Wound diameter, weight, and blood glucose levels of all the mice groups were measured at the start and end of the experiment. Additionally, qPCR was performed to analyse the expression level of some crucial genes (MMP-2, NANOG and OCT4) associated with wound healing. Overall, the results showed reduced wound size, blood glucose level, and weight restoration in ADSCs-CM treated mice as compared with control groups. Furthermore, ADSCs-CM also modulated the gene expression of MMP-2, NANOG, and OCT4, contributing to the enhanced wound healing observed in diabetic mice. This suggests ADSC-CM’s potential as an alternative therapeutic option for achieving improved wound healing outcomes in diabetic patients.

Quantitative evaluation of the low-temperature pulsed plasma-wound interaction based on splint model

An atmospheric pressure low-temperature plasma jet has a promising application prospect in biomedical fields due to its low operating temperature and its ability to act in open space. In this paper, a mouse excisional wound splinting (MEWS) model is developed using a BALB/c strain of mice. A pulsed low-temperature plasma jet close to the human temperature is generated to treat the wound of the mouse skin. We have demonstrated that the pulsed low-temperature plasma jet can effectively promote wound healing. The large number of active particles contained in the plasma, such as reactive oxygen species, reactive nitrogen species, etc., may be responsible for its ability to increase the wound healing rate and promote skin tissue regeneration. The MEWS model adopted in this experiment reduces the interference in the evaluation of the wound healing effect caused by skin contraction and wound dressing, and thus is closer to the healing process of human wounds, and the method of wound area acquisition and calculation based on image processing reduces human interference and is more standardized, which can be used to evaluate the effect of the pulsed low-temperature plasma jet on promoting wound healing.

Customized Wrist Immobilization Splints Produced via Additive Manufacturing—A Comprehensive Evaluation of the Viable Configurations

Orthopedic splints are external medical devices designed to support and protect the functions of the human musculoskeletal system from pathological conditions or traumatic events. Tailoring these medical solutions to the morphology of the patient’s limb is essential to ensure a correct and rapid rehabilitation pathway. Although traditional splinting techniques might achieve a unique fit, the procedures are highly dependent on the skill and experience of the medical operator, affecting the quality of the care treatment. In response to the drawbacks associated with traditional splinting techniques, the present article proposed an innovative and structured methodology to manufacture customized wrist immobilization splints, prioritizing simplicity and user-friendliness in fabrication activities. The customized splint manufacturing was based on the integration of reverse engineering (RE) and additive manufacturing (AM) techniques. The research designed a baseline model of a wrist splint, varying over different thickness values and manufacturing materials (ABS, nylon, PLA, PC, PA6-GF25, PA6-CF20). For every splint model, the production times and material costs were assessed. Technical tests were performed via finite element analysis (FEA). The conducted analysis and the resulting charts empower medical operators to select the most appropriate solution, ensuring a well-informed and effective decision-making approach.

Biomimetic hydrogel derived from decellularized dermal matrix facilitates skin wounds healing.

Cutaneous wound healing affecting millions of people worldwide represents an unsolvable clinical issue that is frequently challenged by scar formation with dramatical pain, impaired mobility and disfigurement. Herein, we prepared a kind of light-sensitive decellularized dermal extracellular matrix-derived hydrogel with fast gelling performance, biomimetic porous microstructure and abundant bioactive functions. On account of its excellent cell biocompatibility, this ECM-derived hydrogel could induce a marked cellular infiltration and enhance the tube formation of HUVECs. In vivo experiments based upon excisional wound splinting model showed that the hydrogel prominently imparted skin wound healing, as evidenced by notably increased skin appendages and well-organized collagen expression, coupled with significantly enhanced angiogenesis. Moreover, the skin regeneration mediated by this bioactive hydrogel was promoted by an accelerated M1-to-M2 macrophage phenotype transition. Consequently, the decellularized dermal matrix-derived bioactive hydrogel orchestrates the entire skin healing microenvironment to promote wound healing and will be of high value in treatment of cutaneous wound healing. As such, this biomimetic ddECMMA hydrogel provides a promising versatile opinion for the clinical translation.

Finite Element Analysis of Upper Limb Splint Designs and Materials for 3D Printing.

Three-dimensional (3D) printed splints must be lightweight and adequately ventilated to maximize the patient's convenience while maintaining requisite strength. The ensuing loss of strength has a substantial impact on the transformation of a solid splint model into a perforated or porous model. Thus, two methods for making perforations-standard approach and topological optimization-are investigated in this study. The objective of this research is to ascertain the impact of different perforation shapes and their distribution as well as topology optimization on the customized splint model. The solid splint models made of various materials have been transformed into porous designs to evaluate their strength by utilizing Finite Element (FE) simulation. This study will have a substantial effect on the designing concept for medical devices as well as other industries such as automobiles and aerospace. The novelty of the research refers to creating the perforations as well as applying topology optimization and 3D printing in practice. According to the comparison of the various materials, PLA had the least amount of deformation and the highest safety factor for all loading directions. Additionally, it was shown that all perforation shapes behave similarly, implying that the perforation shape's effect is not notably pronounced. However, square perforations seemed to perform the best out of all the perforation shape types. It was also obvious that the topology-optimized hand splint outperformed that with square perforations. The topology-optimized hand splint weighs 26% less than the solid splint, whereas the square-perforated hand splint weighs roughly 12% less. Nevertheless, the user must choose which strategy (standard perforations or topology optimization) to employ based on the available tools and prerequisites.

Olfactomedin-4 improves cutaneous wound healing by promoting skin cell proliferation and migration through POU5F1/OCT4 and ESR1 signalling cascades

Olfactomedin-4 (OLFM4) is an olfactomedin-domain-containing glycoprotein, which regulates cell adhesion, proliferation, gastrointestinal inflammation, innate immunity and cancer metastasis. In the present study we investigated its role in skin regeneration. We found that OLFM4 expression is transiently upregulated in the proliferative phase of cutaneous wound healing in humans as well as in mice. Moreover, a significant increase in OLFM4 expression was detected in the skin of lesional psoriasis, a chronic inflammatory disease characterized by keratinocyte hyperproliferation. In vitro experiments demonstrated that OLFM4 selectively stimulated keratinocyte proliferation and increased both keratinocyte and fibroblast migration. Using proteotranscriptomic pathway analysis we revealed that transcription factors POU5F1/OCT4 and ESR1 acted as hubs for OLFM4-induced signalling in keratinocytes. In vivo experiments utilizing mouse splinted full-thickness cutaneous wound healing model showed that application of recombinant OLFM4 protein can significantly improve wound healing efficacy. Taken together, our results suggest that OLFM4 acts as a transiently upregulated inflammatory signal that promotes wound healing by regulating both dermal and epidermal cell compartments of the skin.

Mandibular condylar process remodeling in rats with different bite-altering devices.

The objective was to compare different dental splint models and materials for inducing abnormal loading on the gross morphology and histological appearance of the mandibular condylar processes of Sprague Dawley rats. Three different types of dental splints (resin molar, aluminum incisor, stainless-steel incisor) were placed unilaterally to induce occlusal perturbation for 4 weeks. At that time, mandibular condylar processes were assessed by gross appearance and histology. Quantitative measurements were also conducted on the hematoxylin and eosin images for condyle shape. The results showed that although the condylar cartilage was affected by all splint types, the resin molar splint was associated with the most extensive mandibular condylar process remodeling, which was primarily a slant (skewness) of the lateral aspect of the condylar process. Additionally, quantitative measurements on the histological specimens demonstrated that the split and tilt angle of the left (ipsilateral) condylar processes in the resin molar group (124.8 ± 12.7° and 104.1 ± 12.7°, respectively) increased significantly (p < 0.05) when compared to right (contralateral) condylar processes (104.7 ± 5.8°and 91.6 ± 4.4°, respectively). However, no changes were noted on the thickness of the fibrocartilage layer at medial, central, and lateral regions of the condylar process. Another major finding is the high variability of morphology of the naïve animals. Future studies will assess the impact of longer durations of splinting, age, and sex on the remodeling of the mandibular condylar process, allowing for the development of diagnostics and therapies.

3D-MSCs A151 ODN-loaded exosomes are immunomodulatory and reveal a proteomic cargo that sustains wound resolution

IntroductionNon-healing wounds remain a major burden due to the lack of effective treatments. Mesenchymal stem cell-derived exosomes (MSC-Exo) have emerged as therapeutic options given their pro-regenerative and immunomodulatory features. Still, little is known on the exact mechanisms mediated by MSC-Exo. Importantly, modulation of their efficacy through 3D-physiologic cultures together with loading strategies continues underexplored. ObjectivesTo uncover the MSC-Exo-mediated mechanism via proteomic analyses, and to use 3D-culture and loading technologies to expand MSC-Exo efficacy for cutaneous wound healing. MethodsMSC-Exo were produced in either 3D or 2D cultures (Exo3D/Exo2D) and loaded with an exogenous immunosuppressive oligodeoxynucleotide (A151 ODN). Both, loaded and naïve exosomes were characterised regarding size, morphology and the presence of specific protein markers; while IPA analyses enabled to correlate their protein content with the effects observed in vitro and in vivo. The Exo3D/Exo2D regenerative potential was evaluated in vitro by assessing keratinocyte and fibroblast mitogenicity, motogenicity, and cytokine secretion as well as using an in vivo wound splinting model. Accordingly, the modulation of inflammatory and immune responses by A151-loaded Exo3D/Exo2D was also assessed. ResultsExo3D stimulated mitogenically and motogenically keratinocytes and fibroblasts in vitro, with upregulation of IL-1α and VEGF-α or increased secretion of TGF-β, TNF-α and IL-10. In vivo, Exo3D reduced the granulation tissue area and promoted complete re-epithelization of the wound. These observations were sustained by the proteomic profiling of the Exo3D cargo that identified wound healing-related proteins, such as TGF-β, ITGA1-3/5, IL-6, CDC151, S100A10 and Wnt5α. Moreover, when loaded with A151 ODN, Exo3D differentially mediated wound healing-related trophic factors reducing the systemic levels of IL-6 and TNF-α at the late stage of wound healing in vivo. ConclusionOur results support the potential of A151-loaded Exo3D for the treatment of chronic wounds by promoting skin regeneration, while modulating the systemic levels of the pro-inflammatory cytokines.

Resting hand splint model from topology optimization to be produced by additive manufacturing

PurposeThe purpose of this study is to validate a novel model of resting hand splint manufactured by additive manufacturing (AM) and compare it with the traditional model manufactured by high temperature thermoplastic in terms of cost, weight, volume and thermal comfort.Design/methodology/approachA novel resting hand splint model was created from the topology optimization (TO) and analyzed, by finite-element analysis, manufacturing cost and weight, with a traditional resting hand splint. A pilot clinical study was carried out to verify heat diffusion during the use of the two splints.FindingsThe results showed that compared with the traditional model, the novel model reduced the volume of material used by 35.48%, the weight of the orthosis by 17.56% and the maximum surface deformation by 171.17% when subjected to actuation forces. It was also verified that, when manufactured with Nylon by AM, the new model is 1.5 times cheaper than the traditional model made of Polypropylene. The result of the thermographic analysis showed greater temperature variation in the use of the traditional splint (+4.6°C) compared to the temperature variation observed in the nylon splint (2.1°C).Practical implicationsThese results have as clinical relevance the demonstration of the feasibility of manufacturing functional orthoses that are more comfortable, cheaper and lighter than traditional ones.Originality/valueThis study describes the use of TO to manufacture a novel resting hand splint, which was compared with the commonly used traditional splint in terms of mechanical resistance, weight, cost and thermal comfort.

Polydimethylsiloxane-Based Mechanoluminescent Occlusal Splint with the Visualization of Occlusal Force

The occlusal splint is a common medical device that is used to treat temporomandibular disorders. However, the current commercial occlusal splints still have several disadvantages in terms of flexibility, abrasion resistance, and occlusal inspection. In this work, ZnS:Mn2+/polydimethylsiloxane composite elastomers were prepared, and the mechanical and biological properties were investigated to evaluate their potential as occlusal splint materials. The results suggest that compared to the commercial occlusal splint material, ZnS:Mn2+/polydimethylsiloxane exhibits desirable flexibility, improved wear resistance, and excellent biocompatibility and antibacterial properties. In addition, the elastomer shows intense and stable mechanoluminescence under oral conditions with a linear relationship to the applied load. This further provides a visualized approach for the convenient and real-time detection of occlusal high points. Based on the above results, a mechanoluminescent occlusal splint model was fabricated, which is promising to be applied in occlusal splint therapy applications.

Design optimization of sleeve finger splint model using Finite Element Analysis

One of the rehabilitation alternatives for fingers injuries is to use a finger splint. Some existing finger splint models still use excessive material, and the size is not appropriate. The purpose of this study was to propose a customized sleeve finger splint model with a conical shape along with the finger surface from the proximal phalanges to the distal phalanges. Design optimization using topology optimization was carried out to reduce mass and volume while maintaining the stress strength of the model. The stages of the method in this research were modeling design, preprocessing analysis, topology optimization, and postprocessing analysis. Topology optimization design was set with 70% mass in response constraint. The analysis results showed a significant reduction in the model mass of 42.18%, from 6.78 grams to 3.92 grams. Meanwhile, the maximum equivalent stress increased slightly by 3.42%, from 8.12 MPa to 8.4 MPa. Even though there was an increase in equivalent stress after topology optimization, the sleeve finger splint model was still categorized as safe, with a safety factor of 3.39.

Modern Applications of 3D Printing: The Case of an Artificial Ear Splint Model.

Three-dimensional (3D) printing is a leading manufacturing technique in the medical field. The constantly improving quality of 3D printers has revolutionized the approach to new challenges in medicine for a wide range of applications including otoplasty, medical devices, and tissue engineering. The aim of this study is to provide a comprehensive overview of an artificial ear splint model applied to the human auricle for the treatment of stick-out protruding ears. The deformity of stick-out protruding ears remains a significant challenge, where the complex and distinctive shape preservation are key factors. To address this challenge, we have developed a protocol that involves photogrammetry techniques, reverse engineering technologies, a smart prototype design, and 3D printing processes. Specifically, we fabricated a 3D printed ear splint model via fused deposition modelling (FDM) technology by testing two materials, a thermoplastic polyester elastomer material (Z-Flex) and polycaprolactone (PCL 100). Our strategy affords a custom-made and patient-specific artificial ear aligner with mechanical properties that ensures sufficient preservation of the auricular shape by applying a force on the helix and antihelix and enables the ears to pin back to the head.

A Semi-Automatic Method to Create an Affordable Three-Dimensional Printed Splint Using Open-Source and Free Software.

Three-dimensional (3D) printed splints are becoming more feasible in recent years, showing promising lightweight, waterproof, and hygienic designs. A typical procedure to create 3D printed splints is obtaining the geometry of a body segment using a 3D scanner, creating a 3D printable splint model based on the geometry of the body segment, and 3D printing the splint. As technologies of 3D scanning and 3D printing become mature gradually, the main challenge to fabricate 3D printed splint is to create 3D printable splint models. To solve this challenge, researchers have proposed various methods to design 3D splint models. However, most methods require extensive 3D modeling skills that medical professionals are lacking. In this work, a semi-automatic method is proposed to create a 3D printable model. Given the geometry of a body segment obtained through a 3D scanner, the method includes three key steps: (1) create a draft splint lattice structure, (2) optimize the splint structure, and (3) create a 3D printable model based on the optimized structure. All the software adopted for this method is free and readily available, and thus, there is no additional cost to convert from a scanned geometry of a body segment to a 3D printable splint model. Because the majority of the work is done automatically, with initial training, a medical professional should be able to create a 3D printable model using the proposed method, given the geometry of a body segment. The proposed method is demonstrated by creating a 3D printed wrist splint and the demo is uploaded into GitHub, a popular open-source platform.

Direct 3D Printing of a hand splint using Reverse Engineering

The present work is focused on the direct manufacturing of a hand splint using free-open access software and a low-cost three-dimensional printer (3DP). The hand digital model was created using panoramic photos by a common mobile phone camera. The photos were used as input to the “3DF-Zephyr” free software for creating the hand surface model. Then, the hand surface model was transferred into the “Autodesk fusion 360” free software and the surface model of the hand splint was generated and modified according to the design requirements. Sequentially, both hand and hand splint were translated to Stereolithography (STL) files and transferred to open access “MakerBot” 3D printing software in order to prepare the G-codes for 3D printing. A low cost 3D printer was used for building the models while Polylactic acid (PLA) was the material of the customized 3D physical models.

Naoxintong accelerates diabetic wound healing by attenuating inflammatory response

Context Naoxintong (NXT), a prescribed traditional Chinese medicine, widely used in cerebrovascular and cardiovascular diseases, could be effective in diabetic wounds. Objective This study evaluates the wound healing activity of NXT by employing an excisional wound splinting model. Materials and methods NXT was dissolved in saline and given daily by gavage. Wounds were induced at the dorsum of non-diabetic (db/+) and diabetic (db/db) mice and treated with saline or 700 mg/kg/d NXT for 16 days. Wound closure was measured every four days. Extracellular matrix (ECM) remodelling, collagen deposition, leukocyte infiltration and expression of Col-3, CK14, CXCL1, CXCL2, MPO, Ly6G, CD68, CCR7, CD206, p-JAK1, p-STAT3 and p-STAT6 was analysed. Results NXT significantly accelerated rate of wound closure increased from 70% to 84%, accompanied by up-regulation of collagen deposition and ECM at days 16 post-injury. Moreover, NXT alleviated neutrophil infiltration, accompanied by down-regulation of CXCL1 and CXCL2 mRNA expression. In addition, NXT markedly augmented neutrophil efferocytosis. In diabetic wounds, the levels of M1 marker gene (CCR7) increased, while M2 marker gene (CD206) decreased, demonstrating a pro-inflammatory shift. Application of NXT increased M2 macrophage phenotype in db/db mice. Mechanistically, NXT treatment increased expression level of p-STAT3 and p-STAT6 at days 3 post-injury, indicating NXT mediated macrophages towards M2 phenotype and alleviated inflammation in diabetic wounds by activation of STAT3 and STAT6. Conclusions Our study provides evidence that NXT accelerates diabetic wound healing by attenuating inflammatory response, which provides an important basis for use of NXT in the treatment of chronic diabetic wound healing.

Fractured alveolar process displacement evaluation-Effect of the rigidity of wire-composite splints.

Traumatic dental injuries with bone fractures require rigid splinting. The degree of rigidity of the wire-composite splints can be challenging. The aim of this study was to evaluate the effect of the rigidity of different wire-composite splints on alveolar process displacement. A model was created using polystyrene resin to simulate the anterior maxilla, bovine incisors to simulate maxillary incisors, and polyether impression material to simulate the periodontal ligament. An alveolar process fracture was created, while maintaining the labial bone connection with the PDL. Four wire-composite splints (0.3, 0.6, 0.9, and 1.2mm in diameter) were compared with models without splints and with no fracture (n=5). The alveolar process displacement was measured (3 times per model/per splint type) at the fractured and adjacent central incisor regions using a deflectometer (Instron) under functional cycling and static loading (100N). Data (μm) were analyzed using RM ANOVA, followed by Tukey's HSD test (α=0.05). The displacement at the region of the adjacent incisor was not influenced by the splint during static and cycling load (P>.05). The Tukey test demonstrated that the model without splints had significantly higher alveolar process displacement at the traumatized teeth than that of all other splint models (P<.001). Increasing the wire diameter from 0.3mm to 0.9mm significantly reduced the alveolar process displacement (P<.001). No difference was observed between the 0.9mm (P=.123) and 1.2mm (P=.219) wire-composite splints irrespective of the measured tooth regions. The rigidity of the wire-composite splint significantly influenced the mobility of the fractured alveolar process. The use of at least 0.9mm wire was effective in reducing the fractured alveolar process displacement to a level that was similar to the adjacent teeth.

Evaluation of Wound Healing Activity of Salvia haenkei Hydroalcoholic Aerial Part Extract on in vitro and in vivo Experimental Models.

PurposeThe aim of the present study was to evaluate the potential wound healing activity of the hydroalcoholic extract of Salvia haenkei on in vitro and in vivo experimental models.Materials and MethodsPreliminary analytical characterization of the hydroalcoholic extract of Salvia haenkei was made by reversed-phase high performance liquid chromatography (RP-HPLC) that permitted identification of a qualitative fingerprint of the extract of aerial parts. The wound healing activity of the hydroalcoholic extract of Salvia haenkei was evaluated in vitro by the scratch assay on human dermal fibroblasts and human epidermal keratinocytes and in vivo by standardized mouse excisional splinting model. Real-time PCR (RT-PCR) experiments were performed to analyze gene expression levels of inflammatory markers.ResultsThe scratch assay tests showed that the treatment with the hydroalcoholic extract of Salvia haenkei did not induce an increase in the fibroblasts migration rate with respect to the positive control. Instead, the hydroalcoholic extract of Salvia haenkei was effective in improving the wound closure rate on keratinocyte cell cultures with an almost total invasion of the scratch after 48 h of treatment; whereas the positive control, at the same time point, showed only a 67% reduction of the wound size. In vivo experiments showed that the groups treated with the extract of Salvia haenkei completely re-epithelized the wound in 2.7 days, a timing that was comparable with the action of the positive control that took only 2.1 days. Gene expression analysis showed that Salvia haenkei positively regulated the signaling pathway of the nuclear factor-κB (NF-κB) transcription factor.ConclusionThe results suggested that the hydroalcoholic extract of Salvia haenkei induced a clear wound healing effect.