In response: Yamagishi and Imaizumi speculate that kinetics and role of circulating total soluble form of receptor for advanced glycation endproducts (soluble receptor for AGE, sRAGE) may be different from those of endogenous secretory RAGE (esRAGE) in diabetes. They also suggest an alternative interpretation that serum sRAGE level may reflect tissue membrane form of RAGE expression. Currently, 2 different types of sandwich enzyme-linked immunosorbent assays (ELISAs) are commercially available and used for measurement of circulating soluble RAGE proteins: [1] esRAGE ELISA from B-Bridge Int. esRAGE is a splice variant form of RAGE lacking transmembrane domain and secreting into extracellular space and the circulation.1 Sakurai et al developed its specific detection system using a capture monoclonal antibody (Ab) recognizing RAGE-extracellular domain and a detection polyclonal Ab for the esRAGE-specific C-terminal 16 amino acid sequence.2 This ELISA system is not influenced by coexistence of …