Normal Mouse Splenocytes Research Articles

Absorption of serum antinuclear antibodies

The absorption out of antinuclear antibody (ANA) from sera of highly ANA-positive C57BL/6 mice was obtained by different cellular and noncellular factors. A single injection of DNA, liver nuclei, thymocytes, or repeated injections of a soluble material produced by normal mouse splenocytes with (SIRS+) or without (SIRS−) previous stimulation with Con A into ANA-positive mice induced a transient decrease in ANA level, reaching minimal levels at about 14 days after treatment. A similar elimination of ANA was also achieved by in vitro treatment of positive ANA sera from mice or SLE patients with thymocytes, SIRS (+), or SIRS (−). The absorbed ANA could be released by further treatment with DNase, thus indicating that the absorption-out phenomenon involved immune-complex formation.

Dissociation between the adjuvant vs mitogenic activity of a synthetic muramyl dipeptide for murine splenocytes.

SummaryA relatively low-molecular-weight synthetic muramyl dipeptide was found to stimulate antibody formation by normal mouse splenocytes immunized with sheep red blood cells in vitro. This material also resulted in a marked stimulation of “background” antibody-forming cells to the erythrocytes in cultures incubated without antigen. In contrast, the muramyl dipeptide, regardless of concentration, did not induce a marked blastogenic response in vitro, as occurs with adjuvants such as bacterial lipo-polysaccharides. These results suggest that the nonspecific immunostimulatory properties of a low-molecular-weight substance such as the muramyl dipeptide tested, which is commonly found in bacterial cell walls, may not be dependent upon mitogenicity.