The development of Learner Worksheets (LKPD) in biology learning needs to be optimised for the biology learning process. LKPD can be equipped with a 21st century learning model, one of which is Problem Based Learning. The Merdeka Curriculum does not only prioritise intellectual but also spiritual attitudes, therefore, LKPD can be associated with Emotional Spiritual Quotient (ESQ). The purpose of this study was to analyse the development of PBL-based LKPD and LKPD with ESQ nuances. The research method used is the meta-analysis method, which collects similar articles from various national journals from 2019-2023. The sample used in this study was 10 articles related to the development of PBL-based LKPD and LKPD with ESQ nuances. The results of this study obtained an average validity value of 90.8% categorised as very valid while the average practicality value obtained was 90.1% categorised as very practical. Based on the results of the analysis, the development of PBL-based LKPD and LKPD with ESQ nuances meets the criteria of very valid very practical so it is feasible to use in the learning process.