Sperm Motility Research Articles

The association between consumption of ultra-processed foods and sperm quality parameters: a cross-sectional study

BackgroundWhile recent studies suggest a correlation between unhealthy dietary patterns, oxidative stress, inflammation, and male infertility, the potential association between ultra-processed foods (UPFs) and male infertility remains underexplored. Therefore, the aim of the present study was to investigate the association between UPF intake and male infertility by evaluating sperm quality parameters.MethodsThe participants (n = 260) of the current cross-sectional study were recruited from an infertility center in Isfahan Province, Iran. Four semen parameters—such as total sperm motility, sperm concentration, sperm volume, and normal sperm morphology—were evaluated. Also, the participants’ food intake was assessed using a validated 168-item food frequency questionnaire. Moreover, the NOVA system was employed to calculate the UPF index. The association between UPFs and sperm parameters was analyzed using logistic regression.ResultsIn the crude model, no significant associations were observed between the second and last tertiles of UPFs with abnormalities in sperm concentration, total motility, and morphology (p > 0.05 for all). However, after adjusting for age, marriage duration, body mass index, physical activity, depression, anxiety, stress, energy intake, cigarette history, and mineral and vitamin supplements, a significantly higher association was identified between the second tertile of UPFs and abnormalities in sperm concentration (odds ratio (OR) = 3.962, 95% confidence interval (CI): 1.345–11.670, p = 0.013).ConclusionsIn conclusion, although the analysis did not find significant associations between UPF consumption and impaired sperm motility and morphology, it revealed significant trends linking higher UPF intake with lower sperm concentration. If future studies confirm these results, they could aid in designing interventional and preventive programs aimed at addressing infertility in men of reproductive age in the field of public health.

Protective Effect of Nerolidol on Paclitaxel-Induced Reproductive Toxicity in Rats: Oxidative Stress and Inflammation.

Paclitaxel (PAC), derived from Taxus brevifolia, is used to treat solid tumours but causes reproductive toxicity due to oxidative stress, affecting sperm quality and testicular tissue. Nerolidol (NRL), an antioxidant sesquiterpene alcohol, has not been studied for its potential to reduce PAC-induced reproductive damage. This study investigates NRL's ability to mitigate PAC-induced reproductive toxicity in rats. Forty healthy adult male Spraque Dawley rats were randomly divided into four equal groups (Control, PAC, NRL, PAC + NRL). PAC was given intraperitoneally at a dose of 2 mg/kg once a week for 4 weeks. NRL was given orally at a dose of 100 mg/kg/day for 4 weeks. Control group received PAC and NRL vehicles. After 4 weeks, testis tissue samples were collected, and parameters, including oxidants, antioxidants, sperm motility, density, abnormal spermatozoon ratios and cytokines, were measured. PAC administration increased oxidant levels and decreased antioxidant enzyme activities. Nerolidol mitigated these alterations significantly. Similarly, PAC elevated IL-1, IL-6, TNF-α levels and lowered IL-10 levels, these effects attenuated by nerolidol in the PAC + NRL group. In conclusion, it was determined that PAC induces reproductive toxicity through oxidative stress, and NRL demonstrates potential in ameliorating these effects through its antioxidant activity.

SUMO1 modification reduces oxidative stress and SUMO1ylated AKAP4 degradation affects frozen-thawed boar sperm quality.

Low-temperature injury affects normal physiological function and viability of boar sperm during cryopreservation. Small ubiquitin-like modifier (SUMO) modification of proteins after translation is related to the cell stress response but the relationship between SUMO modification and oxidative stress in freeze-thawed sperm remains unclear. A-kinase ankyrin 4 (AKAP4) and its precursor proAKAP4 are two main proteins in mammalian sperm. Although AKAP4 expression has been studied in many species, its expression in porcine sperm has not been described in detail. In this study, liquid chromatography-mass spectrometry was used to determine the differentially expressed SUMO-modified proteins in porcine sperm after freezen and thawed. The results identified 26 down-regulated SUMO-modified proteins, with AKAP4 identified as one of the target proteins of SUMO1 under sperm stress. In addition, the level of SUMO1 protein increased significantly (P < 0.001) and the level of AKAP4 protein decreased (P < 0.05) after freezing and oxidative stress treatment. Inhibition of SUMO1 modification of AKAP4 protein did not affect its degradation (P > 0.05), indicating that SUMO1 is not involved in the degradation of AKAP4. The inhibition of SUMO1 modification by sperm protein decreased sperm motility (P < 0.05), ATP content, and DNA integrity (P < 0.05). In summary, cryopreservation and oxidative stress can induce SUMO modification of porcine sperm proteins and the modification of sperm protein SUMO1 can help sperm resist oxidative stress; and its role in protecting sperm quality is not via regulating the degradation of AKAP4. These findings provide new insights into the mechanisms underlying SUMO1 modifications during sperm cryopreservation and oxidative stress.

The impact of mercury exposure on male reproduction: Mechanistic insights.

Mercury is a pervasive environmental toxin with significant negative effects on human health. In occupational settings, incidents such as the Minamata and Niigata disease in Japan and the large-scale methylmercury poisoning in Iraq have highlighted the severe health impacts of mercury exposure. It is widely accepted that all forms of mercury including methylmercury and mercuric chloride have the potential to induce toxic effects in mammals, and there is increasing concern about the impact of environmentally relevant levels of mercury on reproductive functions. This review summarizes current knowledge on the mechanisms of mercury toxicity, focusing specifically on its impact on male reproductive health across species. We searched the literature and found that mercury exposure is associated with testicular degeneration, altered spermatogenesis, and Leydig cell deformation. In addition, mercury can disrupt sperm motility, steroidogenesis and interfere with the hypothalamic-pituitary-gonadal axis by generation of reactive oxygen species, inducing mitochondrial dysfunction, epigenetic changes, and DNA damage. At the molecular level, mercury has been found to dysregulate the expression of key steroidogenic and spermatogenic genes, significantly reducing overall fertility potential. However, specific mechanisms of action remain to be fully elucidated. Similarly, comprehensive data on the potential transgenerational effects of paternal mercury exposure are lacking. In this review, we discuss both animal and human studies, and highlight the need for further research due to lack of standardization and control for variables such as lifestyle, immune system function, and exposure concentrations.

Seminal plasma metabolomics and sperm lipidomics profiles of bull semen with different total progressive motile sperm count.

Total progressive motile sperm count (TPMSC) is a reliable index of fecundity evaluation of bull semen. It is an important determinant frozen semen yield and conception rate of females artificially inseminated. Seminal plasma metabolites and sperm lipids are closely related to sperm survival and motility, but their relationship with TPMSC is not well known. In the present study, Simmental bulls with higher (H, n = 6) or lower (L, n = 6) TPMSC (P < 0.01) were selected from a cohort of 100 animals aged 2 to 5 years based on semen quality. Analysis of semen quality and biochemical markers of seminal plasma revealed that H bulls had greater ejaculate volume (P < 0.05), sperm motility, plasma membrane integrity rate (P < 0.01), seminal plasma neutral α-glucosidase (P < 0.05), alkaline phosphatase, acid phosphatase, cortisol and phosphatidylcholine (P < 0.01), and lower sperm malformation rate (P < 0.05) and reactive oxygen species (ROS) (P < 0.01). Semen metabolites and sperm liposome profiles of H and L groups were compared using LC-MS/MS analysis. A total of 120 differentially abundant metabolites (VIP > 1; P < 0.05) and 59 differentially abundant lipids (VIP > 1; P < 0.05) were identified between H and L groups. Oxidative stress, sperm motility and sperm plasma membrane integrity were among the enriched biological pathways. Cyclic ADP-ribose (cADPR), up-regulated in H bulls, is associated with energy for sperm motility and maintenance of membrane stability. Thymidineglycol (Tg), levanbiose, thymidine (Thd), and CE (3M5) were down-regulated in H bulls, and may have negatively affected sperm motility. Correlation analyses revealed that TPMSC and sperm motility were significantly positively correlated with cADPR, while Tg, Levanbiose, Thd and CE (3M5) were significantly negatively correlated with TPMSC and sperm motility. Thus, we speculate that these molecules may be exploited as potential biomarkers for non-invasive evaluation of TPMSC in bull semen.

The impact of vitamin E supplementation on sperm analysis in varicocelectomy patients: a triple-blind randomized controlled trial

ObjectiveTo evaluate the impact of vitamin E supplementation on sperm analysis results in patients post-varicocelectomy.Martials and methodThis single-center, triple-blind, randomized controlled trial was conducted at Imam Reza Hospital, Mashhad, Iran. Ninety male patients, aged 15–25 years, with infertility and varicocele grade 2 or 3, were randomized into two groups. The intervention group received 400 units of vitamin E daily for 3 months, while the control group received a placebo. Sperm analysis was conducted before and 3 months after the intervention. Statistical analyses were performed using SPSS version 23, with significance set at P < 0.05.ResultsA total of 90 patients were enrolled and equally randomized into two groups (n = 45 per group). The mean age was 30.68 ± 6.31 years. Post-intervention, the improvement in sperm motility was significantly higher in the vitamin E group compared to the placebo group (P = 0.03). Both groups showed significant improvements in sperm motility, count, and morphology from pre- to post-intervention (P < 0.001).ConclusionVitamin E supplementation post-varicocelectomy is associated with improved sperm parameters, suggesting potential benefits in the management of male infertility related to varicocele. However, varicocelectomy alone also results in significant improvements.Trial registrationThis study is registered at the Iranian Registry of Clinical Trials (IRCT20200911048689N1). Registered on October 10, 2020.

Best practices for cryopreserving sperm in Nonhuman Primates: a systematic review and meta-analysis

Nonhuman primates (NHPs) are in increasing demand as models for preclinical research. The availability of a highly efficient cryopreservation protocol for use with NHP sperm has potential to significantly alleviate this demand and contribute to rapid increases in overall number of NHPs available for biomedical research. Thus, this systematic review assesses the state-of-the-art in NHP sperm cryopreservation and uses meta-analyses to describe the impact of varied cryopreservation approaches on NHP sperm. We searched the literature deposited in PubMed, Scopus, and Web of Science databases through June 2024 to identify data relevant to the effect of sperm cryopreservation on quality of thawed sperm compared to fresh sperm across NHP species. All original NHP studies reporting sperm parameters both before cryopreservation and after thawing were included for analysis. In total, 32 articles were included for qualitative analysis and not surprisingly, all 32 studies demonstrated negative effects of cryopreservation on sperm parameters, Specifically, motility and viability were significantly decreased in thawed NHP sperm. While the method of sperm collection did not have a significant impact on recovery of motile sperm, significant variability was evident between species. Importantly, recovery of motile NHP sperm was greatest following rapid sperm freezing methods. This analysis provides critical insights into future research directions to enhance and optimize cryopreservation protocols in NHPs.

Chlorantraniliprole (Coragen® 20% SC) exposure induced reproductive toxicity mediated by oxidative stress, apoptosis, and sperm quality deficient in male Wistar rats.

Pesticides can adversely affect reproduction by causing congenital abnormalities, fetal demise, and infertility. The reproductive toxicity of coragen, a modified ryanodine receptor-targeting insecticide with chlorantraniliprole concentrations of 20%, was examined in male rats. Twenty-one healthy male rats were randomly assigned to one of three groups: the control group, two orally administered with low (500mg/kg) and high (1000mg/kg) doses of coragen for 8weeks. Exposure to coragen resulted in significant, dose-dependent changes in male reproductive hormones, steroidogenic enzymes, and an imbalance in the oxidant-antioxidant system. The treated groups revealed significantly higher lipid peroxidation levels than the control group. The effects were accompanied by damage to testicular tissue, modified testicular lactate dehydrogenase, reduced sperm motility and viability, and heightened sperm abnormalities. Elevated levels of pro-apoptotic proteins (caspase-3 and Bax) and decreased levels of anti-apoptotic protein (Bcl-2) provided evidence of apoptosis in both treatment groups. Moreover, coragen induced substantial DNA damage in the testicular tissue. The results indicate that the reproductive impairment caused by coragen may be ascribed to oxidative stress, hormonal disturbance, apoptosis, and damage to testicular DNA and finally might result in infertility and compromised reproductive function.

Impact of Particle Size of Nanoliposomes on the Biological Response of Frozen-Thawed Sperm in Holstein Bulls.

Nanoliposomes could improve the delivering profile of protecting substances in sperm-freezing extenders. This study aimed to assess the impact of nanoliposomes with varying particle sizes and containing 2.5 mM glutathione (GSH) on the post-thaw quality of bull sperm. Ejaculates were obtained weekly from six mature Holstein bulls for six weeks using an artificial vagina. Semen was pooled and mixed with the lecithin-based extender. Semen was pooled and diluted with lecithin-based extenders containing 2.5 mM encapsulated GSH in nanoliposomes with particles in the size range of <50, 50-100, or 100-200 nm and Andromed (control). Intracellular GSH was 4.8±0.14 nmol/108 sperm pre-freezing, decreasing by 58% to 2.8±0.14 nmol/108 sperm post-thawing in control (no differences among treatments). Sperm motility, viability, membrane functionality, apoptotic status, mitochondrial activity, lipid peroxidation, and DNA fragmentation were assessed after cryopreservation. Compared to the control, the 50-100 nm group showed significantly higher viability (72.7%±2.7 vs. 61.2%±2.7) and plasma membrane integrity (82.0%±1.6 vs. 73.3%±1.6). Conversely, the 100-200 nm group showed lower acrosomal integrity (P<0.05 vs. others) and increased DNA fragmentation (P<0.05 vs. 50-100 nm and control). Supplementing the lecithin-based freezing extender with 50-100 nm nanoparticles loaded with 2.5 mM GSH could enhance bull semen cryopreservation.

Clinicopathological and in Silico Insights into Male Infertility: Agerelated Trends and Anti-sperm Antibodies Post COVID-19 Vaccination.

Infertility was often considered a female issue, but male infertility emerged significantly after the Covid-19 pandemic. Hence, assessments are crucial for planning policies on health care and family planning and reasons thereof post vaccinations. The present study was a case-control, dual-centers, prospective study with normal sperm parameters. Semen samples collected by masturbation for idiopathic reasons were conducted at King Abdulaziz University with 133 samples, followed by molecular modeling via interaction between IZUMO1, Alpha2A adrenergic receptor, and Fibroblast growth factor receptor 2 protein with IgA antibody produced post vaccination/infection. The infertile males under 30 (21%), 31-40 (50%), 41-50 (24%) and over 50 (5%), with altered sperm motility grades are A (8.45%), B (11.1%), C (15.8%), and D (59.8%) were reported. Liquefaction times range from 36 to 30 minutes by age, with abnormal sperm percentages between 43.85% and 46.33%. Protein molecular interaction between IZUMO1, Alpha2A adrenergic receptor, and Fibroblast growth factor receptor 2 protein with IgA antibody shows cumulative length of 25.354 Å, 39.049 Å, and 41.999 Å, respectively, with significant interaction between atoms chain, amino acid, marked variation in bond length. Male infertility peaks at 31-40 years, with lowering in men aged 41-50 years, IgA antibody reduced sperm motility, causing immunogenic infertility exacerbated post-Covid-19 vaccination or infection. Interaction of IgA and various receptors produced stable interactive molecules of IgA and proteins on sperm, affecting motility, aliquefication, and abnormal sperm percentage disturbing the normal dynamics of sperm cells opening a new dimension of infertility among males.

HPV-51 or HPV-52 Infection Could Impair Sperm Quality in Infertile Patients: A Preliminary Study on Our Experience from North-Western Italy

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections in all genders worldwide. Its association with male infertility is deeply investigated, although there are conflicting data on the role of the virus in the impairment of semen quality and reduced reproductive outcomes. In this study, we considered 335 semen samples of males (age: 37.63 ± 6.02 years) belonging to infertile couples who did not conceive a pregnancy after 12 months of unprotected intercourse. Residual semen samples, after routine sperm analysis, were used to amplify and type viral DNA. Positive or negative HPV semen samples were compared. In total, 42.51% (139/327) were positive for at least one HPV genotype, and in 54.68% (76/139), positivity was due to a high-risk (HR) genotype. The most prevalent was HPV-16 (16.55%) followed by HPV-52 (10.07%) and HPV-51 (7.91%). Overall, no significant differences emerged in terms of sperm concentration, sperm motility, and morphology between the two groups. However, a considerable reduction in sperm motility was found in the presence of HPV-51 or HPV-52. These data point to the importance of HPV screening in semen analysis to evaluate patients that might have a higher risk of infertility according to the type of HPV genotype.

Age-Related Dynamics in the Conventional, Non-Conventional, and Bacteriological Characteristics of Fresh and Liquid-Stored Porcine Semen

This study strove to investigate the effect of boar age on conventional and non-conventional quality traits of fresh and liquid-stored semen. Sixty boars were allocated into 3 groups: 8–12 months (young); 24–36 months (adult); and 48–60 months (senior). Ejaculates were divided into two parts; the first one was assessed in native state while the second one was extended in the Androstar Plus extender containing gentamycin, stored at 5 °C and evaluated following 72 h. Young animals presented with a significantly lower sperm motility (p &lt; 0.01), membrane and acrosome integrity (p &lt; 0.0001), and mitochondrial activity (p &lt; 0.0001) against adult boars. Significantly higher levels of free radicals and tumor necrosis factor alpha (p &lt; 0.001), interleukin 1 and 6 (p &lt; 0.0001) were found in young boars in comparison to adult boars. The assessment of liquid-stored semen revealed a significantly lower sperm motility, membrane, and acrosome integrity (p &lt; 0.0001) in young boars when compared to adult boars. Moreover, Clostridium difficile, Escherichia coli, Pseudomonas aeruginosa, and Rothia nasimurium remained in liquid-stored semen obtained from young boars, while Corynebacterium sp. and Escherichia coli continued to be identified in samples collected from adult boars. In conclusion, age contributes to the overall quality of fresh as well as liquid-stored boar semen.

Acupuncture mediates the “gut–testis axis” to improve asthenozoospermia

BackgroundAsthenozoospermia is a common cause of male infertility. Studies have shown that sperm quality and motility are affected by the gut–testis axis that can regulate testicular metabolism and function through the gut microbiota and its metabolites. Acupuncture is an important modality of complementary and alternative medicine. It can improve sperm motility, but it remains unclear whether acupuncture can enhance sperm vitality by influencing the gut–testis axis.MethodsIn this study, sperm quality, testicular pathology, and serum hormone levels were assessed using a cyclophosphamide-induced mouse model. Real-time PCR, a western blot analysis, and immunofluorescence techniques were used to assess the effects of acupuncture on the gut barrier and blood–testis barrier functions. In addition, gut microbiome and metabolomics were used to study the impact of acupuncture on the gut microbiota structure, serum, and testicular metabolites in asthenozoospermic mice. Further validation was obtained by performing a fecal microbiota transplantation (FMT).ResultsAcupuncture improved the sperm quality; ameliorated testicular pathology; increased serum testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels; and repaired gut and blood–testis barrier damage in asthenozoospermic mice. The abundances of Bacteroidota, Firmicutes, Faecalibaculum, and Dubosiella were associated with sperm motility, as shown by a gut microbiome analysis. Serum metabolomics revealed that differentially expressed metabolites (DEMs), such as cytosine and N-oleyl-leucine, were closely related to sperm motility. Testicular metabolomics analysis revealed DEMs, such as 5-fluorouridine and 1-acetylimidazole, were also associated with sperm motility. Furthermore, reproductive function improvements in asthenozoospermic mice through acupuncture were achieved via an FMT.ConclusionAcupuncture may alleviate asthenozoospermia symptoms by modulating the gut–testis axis and repairing the gut–testis barrier.

Polylactic Acid Micro/Nanoplastic Exposure Induces Male Reproductive Toxicity by Disrupting Spermatogenesis and Mitochondrial Dysfunction in Mice.

Although considered an "eco-friendly" biodegradable plastic, polylactic acid (PLA) microplastic (PLA-MP) poses a growing concern for human health, yet its effects on male reproductive function remain underexplored. This study investigated the reproductive toxicity of PLA in male mice and its potential mechanisms. To this end, our in vivo and in vitro experiments demonstrated that after degradation in the digestive system, a significant number of PLA-MP-derived nanoparticles could penetrate the blood-testis barrier (BTB) and localize within the spermatogenic microenvironment. Mice exposed to PLA-MPs for a long time exhibited significant reproductive toxicity, evidenced by decreased sperm concentration and motility, increased sperm deformity rates, and disrupted sex hormone levels. Further analysis revealed that PLA impaired BTB, induced mitochondrial dysfunction in the testes, and triggered oxidative stress through excessive ROS production from mitochondria, leading to further testicular damage. Notably, PLA nanoplastics internalized in the mitochondrial sheath and disrupted the mitochondrial structure of sperm, causing dose-dependent impairments in mitochondrial function. Transcriptome analyses further indicated that PLA-MPs disrupted spermatogenesis by inhibiting the expression of key mRNA involved in this process. Collectively, our findings highlight the reproductive toxic effect of biodegradable PLA by damaging BTB and impairing mitochondrial function, which provides insights into the toxicological implications of biodegradable microplastics for mammalian fertility.

Порушення іонтранспортувальних процесів у сперматозоїдах неплідних чоловіків

During the study of the plasma membrane of spermatozoa and its organelles, a great variety of specifically localized ion channels, exchangers, and ATPases was discovered. Although the activity of ion channels and membrane transporters has been studied in detail, their involvement in the mechanisms leading to the dysfunction of male germ cells remains insufficiently elucidated. Numerous scientific works show that the absence of certain transport systems of the plasma membrane due to genetic mutations or their low activity leads to a decrease or loss of sperm motility, morphological changes that worsen the quality of sperm, and is the cause of male infertility. The review examines some ion transport systems that maintain resting membrane potential and ion homeostasis in spermatogenesis. It notes the possibility of using ion channels and membrane transporters as markers to establish the functionality of spermatozoa or as molecular targets for drugs in the treatment of male infertility.

Bisphenol F (BPF) exposure impairs sperm quality and offspring development in male zebrafish.

Bisphenol F (BPF), a substitute for bisphenol A (BPA), is widely used in consumer products, increasing the potential for environmental exposure. Our study investigated the reproductive effects of BPF on adult male zebrafish and explored its toxicological mechanisms, as well as its intergenerational effects. Adult male zebrafish were exposed to BPF concentrations of 0, 50, 500, 2500, and 5000 nM for 21 days. We evaluated sperm cell quantity and quality, hormonal markers testosterone (T) and vitellogenin (VTG), gene expression profiles related to hormone synthesis, metabolism, apoptosis, cell cycle, sexual behavior, and offspring health metrics including survival, development and locomotion. BPF exposure did not significantly affect body weight or gonadal index. However, at 500 and 2500 nM, a significant reduction in sperm count was observed. BPF exposure led to decreased serum T and increased hepatic VTG levels, indicating hormonal disruption. At 50 nM, BPF initiated sperm apoptosis, and at higher doses, it disrupted sperm meiosis, affecting cell distribution. This exposure negatively impacted sperm quality, reduced offspring survival rates, and altered sperm motility in adult fish. Offspring from BPF-exposed groups showed developmental issues, including increased mortality, delayed developmental stages, abnormal tail coiling and heart rate, which correlated with paternal sperm count and quality changes, alterations in T and VTG levels, and cell cycle phase distributions. Our study demonstrated that BPF exposure significantly impacted sperm quality, characterized by reduced sperm count and altered motility patterns, leading to developmental anomalies in offspring. These novel findings highlight the need for further research into BPF's reproductive and developmental toxicity, emphasizing the potential risks to aquatic ecosystems and human health. The observed effects on sperm quality, hormonal balance, and offspring development provide new insights into the reproductive toxicity profile of BPF.

Sperm DNA fragmentation index: limited effectiveness on predicting embryo quality in assisted reproduction technology treatments

PurposePrior sperm DNA fragmentation index (DFI) thresholds for diagnosing male infertility and predicting assisted reproduction technology (ART) outcomes fluctuated between 15 and 30%, with no agreed standard. This study aimed to evaluate the impact of the sperm DFI on early embryonic development during ART treatments and establish appropriate DFI cut-off values.MethodsRetrospectively analyzed 913 couple’s ART cycles from 2021 to 2022, encompassing 1,476 IVF and 295 ICSI cycles, following strict criteria. The WHO guidelines directed the semen analysis, while the acridine orange test (AOT) determined the DFI. Male factors (age, BMI, DFI, infertility duration, sperm parameters) and female parameters (age, BMI, AMH, retrieved oocytes) were evaluated. We also assessed embryological parameters like fertilization rate, cleavage rate, and blastocyst quality. Correlations between DFI and embryo quality were examined and DFI cut-off values were established using ROC analysis.ResultsThe Sperm DFI demonstrated a positive correlation with male age and a negative correlation with sperm motility, concentration, and normal morphology, while showing no relation to BMI. No connection between DFI and embryological parameters in only IVF and ICSI groups was found, but a negative correlation with fertilization rate was seen in all ART cycles. ROC curve analysis revealed a DFI cut-off value of 21.15% having 36.7% sensitivity and 28.9% specificity in predicting high fertilization rate (≥ 80%).ConclusionSperm DFI had a negative correlation with fertilization rate, but limited predictive efficacy and no significant link to other embryological parameters. DFI assessments may improve early embryo development prediction during ART treatments, particularly in older males or those exhibiting poor sperm quality.

Fertility in transgender and gender diverse people: systematic review of the effects of gender-affirming hormones on reproductive organs and fertility.

Transgender and gender diverse (TGD) people seek gender-affirming care at any age to manage gender identities or expressions that differ from their birth gender. Gender-affirming hormone treatment (GAHT) and gender-affirming surgery may alter reproductive function and/or anatomy, limiting future reproductive options to varying degrees, if individuals desire to either give birth or become a biological parent. TGD people increasingly pursue help for their reproductive questions, including fertility, fertility preservation, active desire for children, and future options. Their specific needs certainly require more insight into the effects of GAHT on gonads, gametes, and fertility. This systematic review aims to provide an overview of the current knowledge on the impact of GAHT on gonads, gametes, fertility, fertility preservation techniques, and outcomes. This review was registered in the PROSPERO registry under number CRD42024516133. A literature search (in PubMed, Embase, and Web of Science) was performed with a medical information specialist until 15 November 2024. In all TGD people using GAHT, histological changes have been reported.Using testosterone GAHT, ovarian cortical and stromal changes were reported by various studies. In most studies, persistent activity in folliculogenesis can be concluded based on the descriptions of the follicle count, distribution, and oocyte retrieval yield. However, there may be a negative effect on the fertilization rate in the presence of testosterone. Reports of successful ovarian stimulation, fertilization, pregnancies, and live births have been published, describing cases with and without testosterone discontinuation.After using oestrogen GAHT, testes are reported to be more atrophic, including smaller seminiferous tubules with heavy hyalinization and fibrosis. Spermatogenic levels varied widely from complete spermatogenesis to meiotic arrest with spermatids, to spermatogonial arrest, Sertoli cells only, or even tubular shadows. Oestrogen and anti-androgen treatment causes higher proportions of sperm abnormalities (i.e. low total sperm count, low sperm concentration, poor sperm motility) or azoospermia. However, after cessation, this may be restored. Although knowledge of the effect of GAHT is growing, blind spots remain to be uncovered. Therefore, additional research in this specific population is needed, preferably comparing outcomes before and after the start of GAHT. This may help to reveal the pure impact of GAHT on reproductive functioning. Research suggestions also include investigations into the reversibility of the GAHT effect, especially for those who start transition at a young age. Looking carefully at the presented data on GAHT effects on gonads and gametes, the correct advice is to assess and reassess reproductive wishes and preferences repeatedly, and also to explore individual fertility preservation needs during gender-affirming treatment, given the expanding knowledge and therapy opportunities. Finally, concerns regarding long-term health outcomes and quality of life of children born by the use of gametes preserved after exposure to GAHT require prospective follow-up studies.

IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice

BackgroundAsthenozoospermia (ASZ) accounts for about 20-40% of male infertility, and genetic factors, contributing to 30-40% of the causes of ASZ, still need further exploration. Radial spokes (RSs), a T-shaped macromolecular complex, connect the peripheral doublet microtubules (DMTs) to a central pair (CP), forming a CP-RS-DMT structure to regulate the beat frequency and amplitude of sperm flagella. To date, many components of RSs and their functions in human sperm flagella remain unclear.MethodsWe recruited a cohort of 323 infertile males with ASZ between August 2019 and June 2024. Genetic mutations were identified by whole-exome sequencing. Computer-aided sperm analysis, Papanicolaou staining, and electron microscopy were applied to evaluate the motility, morphology, and ultrastructure of spermatozoa, respectively. Protein mass spectrometry, western blotting, and bioinformatic analyses were performed to identify critical components of mammalian RS1 to model its structure and explore the pathological mechanism of IQUB deficiency. Intracytoplasmic sperm injection (ICSI) was applied for the patient and Iqub−/− mice.ResultsWe identified a novel homozygous IQUB mutation [c.842del (p.L281Pfs*28)] in an ASZ male with normal sperm morphology (ANM), which resulted in the complete loss of IQUB in sperm flagella. Deficiency of RS1, but not RS2 or RS3, was observed in both IQUB842del patient and Iqub−/− mice, and resulted in the reduction of sperm kinetic parameters, indicating the critical role of IQUB in regulating mammalian RS1 assembly and sperm flagellar beat. More importantly, we identified twelve critical components of RS1 in humans and mice, among which RSPH3, RSPH6A, RSPH9 and DYDC1 constituting the head, DYDC1, NME5, DNAJB13 and PPIL6 assembling into the head-neck complex, AK8, ROPN1L, RSPH14, DYNLL1, and IQUB forming the stalk of RS1. Along with the RS1 defect, the IQUB deficiency caused significant down-regulation of the inner dynein arms of DNAH7 and DNAH12, highlighting their nearby location with RS1. Finally, ICSI can effectively resolve the male infertility caused by IQUB genetic defects.ConclusionsWe demonstrate that IQUB may serve as an adapter for sperm flagellar RS1 in both humans and mice and consolidated the causal relationship between IQUB genetic mutations and ANM, further enriching the genetic spectrum of male infertility.

Correlation between seminal α-glycerylphosphorylcholine and semen parameters in infertile patients pre- and post-sub-inguinal micro-varicocelectomy: a prospective study.

Varicocele (Vx) which is the most treatable cause of male infertility, is also associated with low sperm count, decreased sperm motility and increased sperm abnormal morphology. We aimed in the current study to evaluate the correlation between seminal α-Glycerylphosphorylcholine (αGPC) and semen parameters in infertile patients pre- and post- sub-inguinal micro-varicocelectomy. The current comparative prospective study was carried out on 20 male patients who presented to Kasr Al-AinyHospitals from March 2022 to March 2023 as well as 20 healthy controls. The participants were divided into groups as follows: group (1) included fertile normozoospermic men (n = 20) who served as controls. Group (2) included infertile oligoasthenoteratozoospermia (OAT) men with varicocele (n = 20). Patients in group (2) were followed up to 3 months after microsurgicalsub-inguinal Varicocelectomy. The examination included an assessment of Vx with scrotal Duplex. Semen analysis wasdone according to the 5thEdition of WHO manual for semenanalysis. The study demonstrates that αGPC level was significantly higher among fertile normozoospermic control group andinfertile OAT men post varicocelectomy when compared to infertile OAT men preoperative (p<0.001). Moreover, it demonstrates that on follow up of infertile OAT group 3 months after sub-inguinal micro-varicocelectomy, all semen parametersshowed significant improvement compared to the corresponding semen parameters pre-operatively among Vxs grade II andgrade III (p<0.001, p<0.001, respectively). A significant positive correlation was found between αGPC level and semen parameters including sperm normal forms, sperm count andsperm motility. Using ROC curve, αGPC protein showed a sensitivity of (100%) and a specificity of (100%) at cut off value(≤ 1.975 pg/ml) in differentiation between infertile OAT patients with Vx and control fertile normozoospermic men (p<0.001). αGPC may play an important role in infertility in men with Vx and correction of Vx improves the seminal αGPC level.