Complete faunistics was compiled in countries surrounding the Czech Republic and Slovakia. The latest checklist of the bee fauna from the then Czechoslovakia being published well over a decade ago, the aim of this paper is to up-date Kocourek’s checklist (hereinafter referred to as only ”the List”) from 1989.In the present paper, inaccuracies occurring in Kocourek’s checklist (e.g. using of junior synonyms or homonyms, spelling of names, incorrect species distribution, etc.) are corrected. In addition, new records according to literature data, findings communicated by colleagues, or own records, are summarised.The compilation of this faunistic list required checking of over 750 names of the species group and more than 140 supraspecific names to be used valid and available names. The faunistic revision results in the following findings.In total, 431 bee species range the Bohemian territory (occurrence was approved); of that, the following species are newly included: 4 species according to records (captured or checked specimen(s)) and 3 species as per literature data. Five species are conditionally removed from the Bohemian fauna, ten species are missing, eleven species are removed from the checklist, and the occurrence of one species is potential.Altogether 543 species range the Moravian territory; in that number, the following species are newly included: 17 species according to records, and 6 species according to literature data. Eight species are conditionally removed from the Moravian fauna, one species is missing, six species are removed from the checklist, and the occurrence of five species is potential.A total of 625 species range the Slovak territory; of that, the following species are newly included: 17 species according to records, and 14 species according to literature data. Seventeen species are conditionally removed from the Slovak fauna, no species is missing, ten species are removed from the checklist, and the occurrence of thirteen species is potential. Therefore, more intensive faunistic research is recommended in the Slovak territory.Altogether 655 species range the Czech Republic and Slovakia; of that number, 555 species occur in the Czech Republic, and 625 species occur in Slovakia. It results in finding that the diversity of the bee fauna in the Czech Republic and Slovakia can be regarded as comparablely equal with their neighbouring countries regarding the structure and geographic position of the both republics.The most important records of species are as follows: Hylaeus trinotatus (Pérez, 1895) [Slovakia], Andrena pontica Warncke, 1972 [Moravia], Halictus resurgens Nurse, 1903 [S], H. smaragdulus Vachal, 1895 [M, S], Lasioglossum bavaricum (Blüthgen, 1930) and L. cupromicans (Pérez, 1903) [S], L. lissonotum (Noskiewicz, 1926) [M, S], Sphecodes majalis Pérez, 1903 and Melitta wankowiczi (Radoszkowski, 1891) [S], Megachile flabellipes Pérez, 1895 [M], Coelioxys alata Förster, 1853 [S], Anthidium septemspinosum Lepeletier, 1841 and Melecta aegyptiaca Radoszkowski, 1876 [M], Pyrobombus (Cullumanobombus) semenoviellus (Skorikov, 1910) [Bohemia] and P. (Melanobombus) sicheli (Radoszkowski, 1859) [M, S].Pseudoanthidium scapulare (Latreille, 1809) – nom. et sp. rev. – is not conspecific with P. lituratum (Panzer, 1801). Eucera polonica Ruszkowski, 1994 is a junior subjective synonym to E. pollinosa Smith, 1854.