Iron fulfillment refers to an attempt to achieve the ideal conditions of every human body’s metabolism stability, so it is crucial, especially for women considering the monthly cycle that reduces it. Dual role existence as an additional burden for working women also makes female workers vulnerable to work stress and job burnout. The nursing sector at Sultan Agung Islamic Hospital Semarang, which is dominated by women with an increasingly workload due to being one of the referral centers for health facilities on the north coast and locations that often experience flooding, is very close to excessive job fatigue. This study aims to analyze iron tablet supplementation’s effect on female nurses’ fatigue levels in an inpatient unit. This research is quantitative with a quasi-experimental method and one group pre-test and post-test design. This study populations were female nurses in the inpatient unit at Sultan Agung Islamic Hospital Semarang, with 40 people as a sample using the purposive sampling method. The dependent variable in this study was work fatigue level measured using reaction time application to determine reaction speed, while the independent variable was iron tablet supplementation for 4 weeks with 2 consumptions each week. The results showed that there was an increase in the reaction speed of respondents with an average of 44.8 milliseconds. The statistical test used was a Paired T-test test with measurement results showing a p-value of 0.0001 (p<0.05), so it is known that there is a difference before and after supplementation. It can be concluded that iron tablet supplementation has an effect in increasing the reaction speed of respondents which means a decrease in work fatigue level.
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