Spectroscopic Methods Research Articles

Generating luminescent Graphene quantum Dots from Tryptophan: Fluorosensors for hydrogen peroxide in cancer cells

Herein, we report a single step synthesis of highly fluorescent Graphene Quantum Dots (GQDs) using tryptophan and glycerol as precursors via pyrolysis. The morphological and functional characterization of the prepared GQDs was performed using PXRD, FTIR, TEM, XPS and zeta potential measurements. The prepared GQDs found their practical application in ultrasensitive detection of an emerging potential cancer biomarker, H2O2, by exploiting the fluorescence quenching behaviour of H2O2. To evaluate the detection sensitivity, a series of various concentrations of H2O2 was spiked to biomatrices like, serum and MCF-7 (human breast cancer cell line) cell lysate medium. A remarkably low limit of detection (LOD) was found in serum medium (139.5 pM) which further improved in MCF-7 cell lysate medium (LOD 61.43 pM). Moreover, the sensing capacity of the GQDs was further validated in presence of various physiological variables such as glucose, cholesterol, insulin and nitrite. Sensing assay was also carried out in HaCaT (human keratinocyte cell line) cell lysate medium to compare the performance of our prepared sensor but the non-linearity of the F0/F versus H2O2 concentration plot pointed towards the conduciveness of the MCF-7 cell lysate medium for sensitive detection of H2O2.The mechanism behind the sensing was also explored using spectroscopic methods.

Synthesis, crystal structure, theoretical calculations, and corrosion study of thiazine[1,5]benzodiazepine

A derivative of 1,5-benzodiazepine, specifically 2-methyl-6-phenyl-2,3-dihydro-1H-[1,3]thiazino[3,2-a]-1,5-benzodiazepin-1-one (TBD), was synthesized by a cyclization [3 + 3] reaction involving the 4-phenyl-1,3-dihydro-2H-1,5-benzodiazepine-2-thione and methacryloylchloride. The structure of TBD was characterized through various spectroscopic methods, including FT-IR, 1H NMR, 13C NMR, and HRMS, also it was confirmed by X-ray analysis. The corrosion inhibitory effectiveness of TBD for mild steel (MS) in 1 M HCl acid was inspected by the exploitation of electrochemical impedance spectroscopy (EIS) and potentiodynamic techniques. Monte Carlo (MC) simulations were employed to study the adsorption behavior of TBD on the Fe (110) surface, providing insights into its inhibitory efficiency against MS corrosion. The simulations were also utilized to calculate the energy of adsorption of the inhibitor onto MS. A comprehensive theoretical investigation, employing density functional theory (DFT) at the B3LYP/6–311 G (d,p) level, was carried out to validate the experimental findings regarding the inhibition properties of the synthesized product and to elucidate the corrosion inhibition mechanism.

Synthesis, bioactivity, and molecular docking of pyrazole bearing Schiff-bases as prospective dual alpha-amylase and alpha-glucosidase inhibitors with antioxidant activity

A series of pyrazole-Schiff base derivatives, 3a-i, were synthesized by reacting arylamines 2a-i with pyrazole aldehyde 1, utilizing two catalysts: ammonium chloride (catalystA) or acetic acid (catalystB). The compounds were analyzed using spectroscopic methods, including 1H and 13C NMR, along with high-resolution mass spectrometry. Their antidiabetic and antioxidant activities were assessed through various in vitro assays, encompassing evaluation of inhibitory effects on α-glucosidase, α-amylase enzymes and determination of reducing power, free radical scavenging activity. The most promising in vitro antidiabetic results were also observed with analogs 3c (α-amylase IC50 = 19.57 ± 0.07 µM, α-glucosidase IC50 = 17.13 ± 0.28 µM) and 3h (α-amylase IC50 = 22.50 ± 0.06 µM, α-glucosidase IC50 = 20.75 ± 0.17 µM). Both products 3c and 3h revealed activity near to the standard acarbose (IC50 (α-amylase) =16.28 ± 0.24 µM, IC50 (α-glucosidase) =13.19 ± 0.26 µM). Among all compounds, 3c and 3h exhibited the highest transition metal reducing activity at levels of 75.27 ± 1.99 and 78.42 ± 0.58 µM Trolox equivalents (TE), respectively, along with the most significant free radical scavenging effects at levels of 73.79 ± 0.11 and 72.98 ± 0.33 µM TE, respectively. The antioxidant results of the current set of pyrazole-Schiff base derivatives 3a–i indicated their potential benefits as free radical scavenging and metal-reducing agents, thus proving their antioxidant capability. Their higher antidiabetic and antioxidant activities compared to other compounds might be explained by the presence of a free phenolic hydroxy group in 3c and a benzothiazole motif in 3h. These findings also lend support to the multifaceted biological functions associated with their antidiabetic activity, including their antioxidant activity. In silico molecular docking studies confirm the antihyperglycemic impact, elucidating the nature of interactions between 3c and 3h and HPA/HLAG catalytic sites.

The total synthesis of Wulfenioidin F

Zika virus, an RNA virus that can cause severe damage to the human body, has long lacked an effective drug. Scientists have discovered recently that Wulfenioidin, a plant distributed in southern China, contains a compound in its root that holds the potential to become an anti-Zika drug. Through extraction, multiple kinds of Wulfenioidin were discovered and their structures were characterized through various spectroscopic methods. However, there isnt a total synthesis pathway for Wulfenioidin. This essay focuses on Wulfenioidin F and provides a full synthesis pathway for the molecule. The route begins with the synthesis of two fragments 6-(2-bromo-6-methylphenyl)-6-methoxy-2-methylhexan-2-ol and 5-(propan-2-yl)-3-(I^5-diazynylidene)-3,4-dihydro-2H-pyran-2,4-dione, which then undergo a convergent coupling to form an eight-carbon ring. An intermolecular Diels-Alder reaction combines the benzyne and pyrone together, forming the final product and completing the total synthesis.

Five anti-inflammatory compounds from the resins of Liquidambar orientalis

Five undescribed compounds, including a triterpenoid (1), three phenylpropanoids [(±)−2 and 4], and an aromatic compound (3), as well as six known analogues (5–10), were isolated from the resins of Liquidambar orientalis Mill. Their structures, including absolute configurations, were determined by using spectroscopic and computational methods, and the five new compounds displayed anti-inflammatory activities in LPS-induced RAW264.7 cells.

Effect of surfactants on the luminescence, bonding, and catalytic properties of CaWO4 spheres

BackgroundThis work focuses on improving the luminescence and catalytic properties of CaWO4 spheres by manipulating their electron density distribution. The goal is to enhance their suitability for optoelectronic applications and to increase their efficiency in degrading the industrial dye methylene blue (MB). The study utilizes a co-precipitation technique and the addition of three surfactants to synthesize phase-pure CaWO4 with a tetragonal crystal structure. MethodsThe synthesis involved a co-precipitation technique with simultaneous addition of three surfactants. Extensive characterization employed various analytical and spectroscopic methods to assess structural, morphological, luminescent, and catalytic properties. Particularly, the use of cetyltrimethylammonium bromide (CTAB) surfactant induced intense green emission around 500 nm attributed to luminescent center WO42−. Solar irradiation evaluated catalytic performance, with CaWO4: CTAB degrading methylene blue remarkably in just 40 min under natural sunlight. Significant FindingsThe research yielded a CaWO4 material, particularly when assisted by the CTAB surfactant, exhibiting intense green luminescence, making it promising for optoelectronic applications. Furthermore, this material demonstrated remarkable catalytic performance in degrading methylene blue under solar irradiation, suggesting its potential in environmental remediation. The study also delved into electron density distribution by examining differences in bond lengths and mid-bond electron density within a single unit cell, shedding light on the material's underlying properties.

The interaction mechanisms of tetrahydrocurcumin on alcohol dehydrogenase: Insights from enzymatic activity assays, spectroscopic methods and computational techniques

Tetrahydrocurcumin (THC), a major metabolite of curcumin, is renowned for its potent antioxidant and anti-inflammatory properties, including preventing liver injury from alcohol. Alcohol dehydrogenase (ADH), an essential enzyme in alcohol metabolism, is significant in the development of alcohol-induced diseases. Hence, enzymatic activity assays, spectroscopic methods, and computational techniques were employed in this study to explore their interaction. The results indicated that THC can competitively inhibit ADH activity, as inferred from the kinetic parameter values. Meanwhile, THC spontaneously entered the substrate-binding cavity of ADH to form a binary complex, and surrounded by Thr178, Leu57, Ile318, Leu141, Glu68, Leu116, Phe93, Val294, and Asp49. The Ka value of this binding was 1.724 ± 0.017 × 104 M−1 at 298 K. This binding process was driven by hydrogen bonds and van der Waals forces; additionally, the π electrons in THC were significant for the stability of the ADH-THC complex. The presence of THC led to a decrease in the α-helix content of ADH, resulting in a looser secondary structure. Furthermore, molecular dynamic simulations revealed that Glu68, Leu116, Phe93, Val294, and Asp49 made significant contributions to the binding process. The inhibition of ADH catalytic activity may be primarily attributed to the interaction between THC and the Val294 residue.

Tribological performance and mechanism of graphite, hBN and MoS2 as nano-additives in palm kernel oil-based lubricants: A comparative study

Layered nanoparticles have received considerable interest in tribological applications owing to their outstanding tribological performances, including in vegetable oil-based lubricants. Despite the advantages of vegetable oil-based lubricants over petroleum-based lubricants, the limited manufacturing scale of vegetable oil-based lubricants restricts their more comprehensive implementation in various industries. In order to gain further insights into the exquisite nano-additive properties of layered nanoparticles, this research was conducted to explore the tribological performance of palm kernel oil-based lubricants combined with three different layered nanoparticles, namely graphite, hexagonal boron nitride (hBN), and molybdenum disulfide (MoS2), using a four-ball tribotester. The four-ball tribotester analysis indicated that the wear scars lubricated with graphite, hBN, and MoS2 nanolubricants exhibited a lower coefficient of friction (COF) and wear scar diameter (WSD) than the base lubricant. The findings also showed that the MoS2 nanolubricant had the best friction reduction and anti-wear performance, with a 24.9% drop in COF and a 12.3% drop in WSD. It was followed by the hBN and graphite nanolubricants. To fully understand the lubricating mechanisms involved, the worn surfaces of the tested balls were characterised using various microscopic and spectroscopic methods. Accordingly, the structural changes that occurred during sliding were shown to affect the formation of protective tribofilm. In summary, the findings in this study suggest that the formulated layered nanolubricants possessed exceptional tribological performance, which was contributed by the inherent properties of the individual nanoparticles and the formation of effective tribofilms.

Development of tungsten trioxide nano-flakes intercalated on tannic acid-functionalized reduced graphene oxide for flexible acebutolol sensors

Acebutolol (ACE) is commonly used to treat hypertension and high blood pressure. Large doses of ACE can have adverse effects with potentially life-threatening consequences. It is, therefore, essential to develop a simple, low-cost, reliable, and reproducible device for detecting ACE in biofluids. This study explores the potential of unique two-dimensional nano-flakes, such as tungsten trioxide (WO3). Graphene oxide (GO) typically exhibits lower electrical conductivity than pristine graphene due to the presence of oxygen-containing functional groups that interfere with the π-conjugated structure. Functionalizing GO with tannic acid (TA) can partially reinstate the π-conjugation and limit the amount of oxygen, resulting in enhanced electrical conductivity. Ultrasonic techniques were utilized to create WO3 NFs@TA-rGO, and a range of spectroscopic and microscopic methods were applied to examine the formation of the resulting WO3 NFs@TA-rGO nanocomposites. Under optimal conditions, modified sensors resulted in lower limits of detection (0.0055 μM) and good sensitivity (0.40 μA μM−1 cm−2). They also exhibited a broad linear range spanning from 0.009 to 568.6 μM. Fabricated sensors have significant anti-interference properties with high specificity and excellent storage stability (RSD = 4.3 %), reproducibility (RSD = 3.9 %), and repeatability (RSD = 3.3 %). Ultimately, the sensor's efficacy was confirmed through the successful detection of ACE in biological samples (with recoveries ranging from 99.1 to 99.6 %). Lastly, this study highlights the substantial potential of ACE detection and extends its applications in biomedical diagnostics and pharmaceutical research.

Connecting Stories: Investigations of a Seventeenth-century Mercator Hand-painted Map

ABSTRACT A seventeenth-century hand-painted map entitled Walachia, Servia, Bulgaria, Romania, printed with the original copper plate engraved by Gerardus Mercator, was investigated using portable, non-invasive spectroscopic and imaging methods. X-ray fluorescence spectrometry, UV fluorescence imaging, and short-wave infrared hyperspectral imaging were correlated to characterize pigments, differences in materials, previous interventions, and specific types of degradation. The results showed evidence for the use of vermilion, red lead, a green copper-based pigment, a gray-blue cobalt-based pigment, a chromate-based yellow pigment, and gold leaf, and even inferred the possible use of black ferro-gallic ink, thus highlighting both original materials and the likely presence of at least one previous restoration from the nineteenth century. Hyperspectral imaging was able to discriminate the most significant coloring materials, while UV fluorescence imaging suggested the use of organic material, both as a coating for the gold leaf and as a component in the mixture to temper the vermilion and the green copper-based pigments.

Chirality and pH Influence the Self-Assembly of Antimicrobial Lipopeptides with Diverse Nanostructures.

Chirality plays a crucial role in the self-assembly of biomolecules in nature. Peptides show chirality-dependent conformation and self-assembly. Lipidation of peptides occurs in vivo and has recently been exploited in designed conjugates to drive self-assembly and enhance bioactivity. Here, a library of pH-responsive homochiral and heterochiral lipidated tripeptides has been designed. The designed lipopeptides comprise homochiral C16-YKK or C16-WKK (where all the amino acids are l-isomers), and two heterochiral conjugates C16-Ykk and C16-Wkk (where the two lysines are d-isomers). The self-assembly of all the synthesized lipopeptides in aqueous solution was examined using a combination of spectroscopic methods along with cryogenic-transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS). Interestingly, it was observed that at acidic pH all the lipopeptides self-assemble into micelles, whereas at basic pH the homochiral lipopeptides self-assemble into nanofibers, whereas the heterochiral lipopeptides self-assemble into nanotapes and nanotubes. A pH switch was demonstrated using a thioflavin T fluorescence probe of β-sheet structure present in the extended structures at pH 8. We demonstrate that both chirality and pH in lipopeptides influence the self-assembly behavior of the model tripeptides, which also show promising bioactivity. Good cytocompatibility is observed in hemolytic assays and antimicrobial activity against both Gram-negative and Gram-positive bacteria is shown through the determination of minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values and live/dead bacteria staining assay.

Synthesis, electrochemical, biological and catalytical studies of half-sandwich Ru(II)-NSHC complexes bearing benzothiazol-2-ylidene

The half-sandwich ruthenium(II)-NSHC complexes (2a-l) bearing benzothiazol-2-ylidene were synthesized by in situ deprotonation of N-substituted benzothiazolium salts (1a-l) with Ag2O and of [(p-cymene)Cl2Ru]2, in dichloromethane under mild conditions. However, 1m and 1n under the same conditions gave N-coordinated Ru(II) complex (3). The structures of the compounds were elucidated by NMR, FT-IR, UV–Vis, HR-MS spectroscopic methods and elemental analysis. In addition, the structures of 2k and 3 were studied by X-ray crystallography. The electrochemical properties of the complexes were investigated. Ru(II)-NSHC complexes have been used as catalysts in the transfer hydrogenation of carbonyls to secondary alcohols in the presence i-PrOH/KOH. Additionally, the effects of structural differences arising from different alkyl groups on the nitrogen atom in the benzothiazole skeleton in Ru(II)-NSHC complexes on anticancer, antifungal and antimicrobial activity were also examined.

New prospective insecticidal agents based on N-(arylcarbamothioyl)arylmide derivatives against Spodoptera frugiperda: Design, Synthesis, Toxicological, Biological, Biomedical Studies and Antibacterial Activity.

In this work, a novel series of N-(arylcarbamothioyl)arylmide) 2-11 were synthesized by treating One-Pot three-multicomponent of Aroyl chloride ammonium isothiocyanate and amine compounds under refluxing conditions. Using spectroscopic methods, the chemical structure of the novelty developed compounds were investigated. After five days, the proposed derivatives' insecticidal bioassay was assessed using the median lethal concentration (LC50) against the second & fourth larvae of Spodoptera frugiperda as toxicity agents. The findings showed that, to varying degrees, every tested substance exerted insecticidal effects on S. frugiperda larvae in both of their instars. Compound 9 was the most poisonous of them all, having an LC50 against larvae in their second and fourth instars of 60.45 and 123.21 mg/L, respectively. Additionally, a few biological and biochemical characteristics of the substances that were generated in a lab setting were also looked at. Furthermore, this work discusses how to discover novel compounds that may one day be employed as insecticidal agents. Finally, all the designed components were monitored for their antibacterial effectiveness toward both Gram-positive & Gram-negative bacteria.

Chrysosaxillins A-E, cucurbitane triterpenoid derivatives from Chrysosplenium axillare Maxim. (Yajima) and their cytotoxic activities

Chrysosplenium axillare Maxim. is used in traditional Tibetan medicine for the treatment of various human diseases, such as fever, headache, cholecystitis, acute icterohepatitis and acute liver necrosis. In this study, five new cucurbitane triterpenoid derivatives, chrysosaxillins A-E (1–5), along with three known structurally related compounds (6–8) have been isolated from whole herb of C. axillare. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR, HRESIMS, UV, IR, ECD and single-crystal X-ray diffraction. All isolates were evaluated for cytotoxic activities against four tumor cell lines including PC-3, A549, MCF-7, and HepG2. The results discovered that compound 1 possessed the most potent cytotoxicity against A549 cells with IC50 value of 0.05 μM, while compounds 2 and 4 have mild cytotoxicities against cells tested with IC50 values ranging from 8.78 to 41.72 μM. Our study suggests that C. axillare might serve as a valuable source of cucurbitane triterpenoids potentially useful for the development of new anti-tumor agents and support its use as a crop benefits to local economic.

Chemical reactivity, molecular electrostatic potential, FTIR, NMR, in vitro, and in silico studies of mannopyranoside derivatives: 3-Nitrobenzoylation leads to improve antimicrobial activity

This investigation Extensively explored the synthesis and potential therapeutic applications of derivatives derived from methyl α-d-mannopyranoside. It encompasses a wide range of evaluations, including antimicrobial assessments, molecular docking, dynamic simulations, and ADMET analysis. Spectroscopic methods (FTIR, 1H NMR, 13C NMR, mass) were used to confirm the structures of the synthesized mannopyranoside derivatives. This study assessed the antibacterial activity of the strains against a range of both gram-positive and gram-negative bacteria and revealed significant inhibitory effects. In addition, the mannopyranoside derivatives exhibited significant antifungal activity. Additionally, we studied how to improve the thermal, frontier molecular orbital (FMO), and molecular electrostatic potential (MEP) properties of mannopyranoside and its acylated analogs by using density functional theory (DFT). Molecular docking studies provided further evidence of the advantageous antibacterial effects of the mannopyranoside derivatives 3, 6, and 7 against the penicillin-binding protein PBP2a (1VQQ) from the methicillin-resistant binding protein Staphylococcus aureus. The docking results were confirmed via molecular dynamics (MD) simulations, which provided a dynamic view of protein stability. The conformational stability increased in the simulations, and the values of the solvent-accessible surface area (SASA) and radius of gyration (Rg) were found to be consistent and stable. Reduced root mean square fluctuations (RMSFs), facilitated by strong hydrogen bonding interactions, characterize ligand–protein interactions. The toxicological and pharmacokinetic profiles of the molecule were assessed using ADMET analysis. The results showed that the molecule had favorable drug-like features, making it a strong candidate for further development. Overall, these findings indicate that 3-nitobenzoylated mannopyranoside derivatives exhibit considerable potential as therapeutic agents for treating microbial infections.

Searching for a relationship between the elemental composition of archaeological bones and the occurrence of caries.

Although the macroscopic assessment of dental caries and the assessment of bone elemental composition are quite different, efforts can be made to identify commonalities in the assessment of health and nutritional quality. Both indicators are correlated with dietary habits and are dependent on taphonomic processes occurring in the postmortem substrate. However, teeth exhibit structural resilience of their hard tissues to adverse environmental factors. The aim of the study was to establish a correlation between the elemental composition of bones and the presence of carious lesions. The study material consisted of the following skeletal parts: 161 permanent teeth from 36 individuals and bridge fragments of 36 ribs. The presence of caries was assessed visually using a modified International Caries Detection & Assessment System (ICDAS II) scale. The rib samples were subjected to elemental analysis (zinc (Zn), iron (Fe), magnesium (Mg), calcium (Ca), phosphorus (P), strontium (Sr), barium (Ba)) using spectroscopic methods. The odontological and chemical analyses did not reveal any statistically significant relationships between the Ca/P diagenesis index and dental features. Postmortem tooth loss showed a weak correlation with the diagenesis index. Discoloration, cracks and flaking of the dental crown surfaces may be associated with the intensity of Ca/P diagenesis. However, no significant correlation was found between these phenomena. Among other elements, only Zn levels exhibited a correlation with the caries index.

Ganospirones A−G, seven undescribed spiro-meroterpenoids from Ganoderma lucidum and their biological activities

Ganoderma lucidum, a medicinal mushroom with a long history in traditional Chinese medicine, is widely used for chronic diseases. Ganospirones A−G (1–7), seven pairs of undescribed spiro-meroterpenoids, were isolated from the fruiting bodies of G. lucidum. Their structures including absolute configurations were characterized by using NMR spectroscopic data, ECD computational and X-ray diffraction methods. The anti-inflammatory and anti-renal fibrosis activities of the meroterpenoids 1–7 were tested, and the results revealed that (−)-2 and (+)-2 could inhibit iNOS expression in lipopolysaccharide-induced RAW264.7 cells at 20 μM.

A three-channel biosensor based on stimuli-responsive catalytic activity of the Fe3O4@cu for on-site detection of tetrodotoxin in fish

Tetrodotoxin (TTX), a lethal neurotoxin, poses a grave threat to human health. The available spectroscopic methods suffer from limitations such as complex procedures and inadequate on-site capabilities. In this study, we proposed a method using Fe3O4@Cu as a catalytic biosensor combined with SERS, colorimetry and image processing for TTX detection. Integrating the aptamer amplifies the specificity of the system and masks the catalytic activity of Fe3O4@Cu. The catalytic efficiency of Fe3O4@Cu in the H2O2-TMB reaction can quantify the concentration of TTX in the system. Consequently, oxidation of TMB (oxTMB) led to the generation and change of signals for SERS, colorimetry and image processing, enabling a three-channel quantitative detection of TTX. Under the optimal conditions, the detection limit of established SERS, colorimetry and image processing were 0.055, 2.127 and 0.243 ng/mL, respectively. This three-channel biosensor was applied to real samples, providing an accurate, stable and adaptable alternative for on-site TTX detection.

Structural insights into the binding interaction between noraucuparin and bovine serum albumin: Implications for antibiotic design and function

Noraucuparin, a biphenyl phytoalexin, emerges as a promising drug candidate. Serum albumin plays a very important role in the in vivo transportation of drugs. The investigation delves into the binding interaction between noraucuparin and BSA employing comprehensive spectroscopic methods and molecular docking technique. UV–vis and FL spectrum results indicated that noraucuparin bond with BSA, and there was a new complex produced at the binding site II in domains IIIA (Ser-453 residue). The dissociation constants Kd from The ITC analysis was found to be 4.63 × 10−5 M at 298 K and 6.67 × 10−5 M at 310 K, showing 310 K is favorable for the combination of noraucuparin and BSA. The negative enthalpy (ΔH) and entropy (ΔS) values for the interaction revealed that the binding behavior was driven by hydrogen bonds. The results obtained from 3D fluorescence and CD spectrum illustrated that noraucuparin could change the secondary structure of BSA. According to molecule docking result, the binding energy of interaction is −7.09 kcal·mol−1. These revelations furnish a nuanced molecular-level comprehension of noraucuparin's mechanism of action on serum albumin. The discernments garnered from this study bear substantial reference significance and practical implications for ongoing lead compound exploration in antibiotics, particularly in vivo contexts.

Physicochemical Properties of Warp Knitted Fabrics Colored with Madder and Gall Oak for Sportswear

ABSTRACT Polyamide/elastane warp-knitted fabrics were separately dyed with gall oak (Quercus infectoria Olivier) and madder (Rubia tinctorum L.). Chromatographic and spectroscopic methods were used to analyze dyed warp-knitted fabrics. A reserved phase high-performance liquid chromatography with the diode-array detection (RP-HPLC-DAD) method was utilized to determine the components of dyes present in the dyed fabrics. Scanning electron microscopy-energy X-ray spectroscopy (SEM-EDX) was utilized for morphological analysis. The undyed and dyed warp-knitted fabrics were characterized by FT-IR. The CIEL*a*b* values of the dyed polyamide-elastane warp-knitted fabrics were measured. Further, in physical testing, the pilling and abrasion resistance of raw and dyed fabrics were examined, and the water contact angle values of all fabrics were measured. Pilling test results were evaluated with a grade of 5 for all fabrics as the best score, and the resistance of fabrics to abrasion was very good. The formation of the fiber-metal-dye complex caused the fabric’s contact angle to drop, giving the fabric a hydrophilic character. The colorfastness characteristics of the dyed fabrics to light, rub, wash, and perspiration have been evaluated. The light fastness scores vary from 3 to 5, and they are satisfactory for using natural dyes to color textiles since they are in the good to moderate range. Overall, the dyed samples displayed a score of 5, which is excellent, on the Gray Scale for washing fastness.