Spectroscopy Mode Research Articles

Observing circumplanetary disks with METIS

Context. Gaining a full understanding of the planet and moon formation process calls for observations that probe the circumplanetary environment of accreting giant planets. The mid-infrared ELT imager and spectrograph (METIS) will provide a unique capability to detect warm-gas emission lines from circumplanetary disks. Aims. We aim to demonstrate the capability of the METIS instrument on the Extremely Large Telescope (ELT) to detect circumplanetary disks (CPDs) with fundamental v = 1−0 transitions of 12CO from 4.5 to 5 μm. Methods. We considered the case of the well-studied HD 100546 pre-transitional disk to inform our disk modeling approach. We used the radiation-thermochemical disk modeling code ProDiMo to produce synthetic spectral channel maps. The observational simulator SimMETIS was employed to produce realistic data products with the integral field spectroscopic (IFU) mode. Results. The detectability of the CPD depends strongly on the level of external irradiation and the physical extent of the disk, favoring massive (~10 MJ) planets and spatially extended disks, with radii approaching the planetary Hill radius. The majority of 12CO line emission originates from the outer disk surface and, thus, the CO line profiles are centrally peaked. The planetary luminosity does not contribute significantly to exciting disk gas line emission. If CPDs are dust-depleted, the 12CO line emission is enhanced as external radiation can penetrate deeper into the line emitting region. Conclusions. UV-bright star systems with pre-transitional disks are ideal candidates to search for CO-emitting CPDs with ELT/METIS. METIS will be able to detect a variety of circumplanetary disks via their fundamental 12CO ro-vibrational line emission in only 60 s of total detector integration time.

Efficient motional-mode characterization for high-fidelity trapped-ion quantum computing

To achieve high-fidelity operations on a large-scale quantum computer, the parameters of the physical system must be efficiently characterized with high accuracy. For trapped ions, the entanglement between qubits are mediated by the motional modes of the ion chain, and thus characterizing the motional-mode parameters becomes essential. In this paper, we develop and explore physical models that accurately predict both magnitude and sign of the Lamb–Dicke parameters when the modes are probed in parallel. We further devise an advanced characterization protocol that shortens the characterization time by more than an order of magnitude, when compared to that of the conventional method that only uses mode spectroscopy. We discuss potential ramifications of our results to the development of a scalable trapped-ion quantum computer, viewed through the lens of system-level resource trade offs.

Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.

The Raman phenomenon is based on the spontaneous inelastic scattering of light, which depends on the molecular characteristics of the dispersant. Therefore, Raman spectroscopy and imaging allow us to obtain direct information, in a label-free manner, from the chemical composition of the sample. Since it is well established that the development of many brain diseases is associated with biochemical alterations of the affected tissue, Raman spectroscopy and imaging have emerged as promising tools for the diagnosis of ailments. A combination of Raman spectroscopy and/or imaging with tagged molecules could also help in drug delivery and tracing for treatment of brain diseases. In this review, we first describe the basics of the Raman phenomenon and spectroscopy. Then, we delve into the Raman spectroscopy and imaging modes and the Raman-compatible tags. Finally, we center on the application of Raman in the study, diagnosis, and treatment of brain diseases, by focusing on traumatic brain injury and ischemia, neurodegenerative disorders, and brain cancer.

Spectromicroscopy Studies of Silicon Nanowires Array Covered by Tin Oxide Layers.

The composition and atomic and electronic structure of a silicon nanowire (SiNW) array coated with tin oxide are studied at the spectromicroscopic level. SiNWs are covered from top to down with a wide bandgap tin oxide layer using a metal-organic chemical vapor deposition technique. Results obtained via scanning electron microscopy and X-ray diffraction showed that tin-oxide nanocrystals, 20nm in size, form a continuous and highly developed surface with a complex phase composition responsible for the observed electronic structure transformation. The "one spot" combination, containing a chemically sensitive morphology and spectroscopic data, is examined via photoemission electron microscopy in the X-ray absorption near-edge structure spectroscopy (XANES) mode. The observed spectromicroscopy results showed that the entire SiNW surface is covered with a tin(IV) oxide layer and traces of tin(II) oxide and metallic tin phases. The deviation from stoichiometric SnO2 leads to the formation of the density of states sub-band in the atop tin oxide layer bandgap close to the bottom of the SnO2 conduction band. These observations open up the possibility of the precise surface electronic structures estimation using photo-electron microscopy in XANES mode.

Authentication of a Stradivarius “Petite Violin” Type from 1723

By correlating the structural–functional dimensional data with a series of archaeometric and chemometric characteristics determined by dendrochronological analysis and by three instrumental techniques (Scanning Electronic Microscopy, coupled with Energy Dispersive X-ray, µ-FTIR Spectroscopy and Thermal Analysis in Dynamic Mode), a Stradivarius violin was authenticated as having been made by one of the two Stradivari sons (Francesco or Omobono) in 1723. It should be noted that the “petite” type violin, which comes from a private collection and was recently purchased on the open market, has the original label and is in a poor preservation state. There is only one revarnishing intervention on the violin, and it is older than 80 years. There have been several attempts at sampling (all of which are under 30 years old) for the wooden support and varnish (from the top cover of the resonance box), but the existence of some analysis results is unknown. The dimensional characteristics of the structural–functional components place the violin in “petite violins”, and it is one of the more than 40 still preserved as an authentic artifact.

Early Release Science of the exoplanet WASP-39b with JWST NIRISS

The Saturn-mass exoplanet WASP-39b has been the subject of extensive efforts to determine its atmospheric properties using transmission spectroscopy1–4. However, these efforts have been hampered by modelling degeneracies between composition and cloud properties that are caused by limited data quality5–9. Here we present the transmission spectrum of WASP-39b obtained using the Single-Object Slitless Spectroscopy (SOSS) mode of the Near Infrared Imager and Slitless Spectrograph (NIRISS) instrument on the JWST. This spectrum spans 0.6–2.8 μm in wavelength and shows several water-absorption bands, the potassium resonance doublet and signatures of clouds. The precision and broad wavelength coverage of NIRISS/SOSS allows us to break model degeneracies between cloud properties and the atmospheric composition of WASP-39b, favouring a heavy-element enhancement (‘metallicity’) of about 10–30 times the solar value, a sub-solar carbon-to-oxygen (C/O) ratio and a solar-to-super-solar potassium-to-oxygen (K/O) ratio. The observations are also best explained by wavelength-dependent, non-grey clouds with inhomogeneous coverageof the planet’s terminator.

Comparison of the effects of AgNPs on the morphological and mechanical characteristics of cancerous cells.

Currently, silver nanoparticles (AgNPs) are the most produced nanoparticles in global market and have been widely utilized in the biomedical field. Here, we investigated the morphological and mechanical effects of AgNPs on cancerous cells of A549 cells and SMMC-7721 cells with atomic force microscope (AFM). The influence of AgNPs on the morphological properties and mechanical properties of cancerous cells were characterized utilizing the force-volume (FV) mode and force spectroscopy (FS) mode of AFM measurement. We mainly focus on the comparison of the effects of AgNPs on the two types of cancerous cells based on the fitting results of calculating the Young's moduli utilizing the Sneddon model. The results showed that the morphology changed little, but the mechanical properties of height, roughness, adhesion force and Young's moduli of two cancerous cells varied significantly with the stimulation of different concentrations of AgNPs. This research has provided insights into the classification and characterization of the effects of the various concentrations of AgNPs on the cancerous cells in vitro by utilizing AFM methodologies for disease therapy.

The Lockman-SpReSO project

Context. Extragalactic surveys are a key tool for better understanding the evolution of galaxies. Both deep and wide-field surveys serve to provide a clearer emerging picture of the physical processes that take place in and around galaxies, and to identify which of these processes are the most important in shaping the properties of galaxies. Aims. The Lockman Spectroscopic Redshift Survey using Osiris (Lockman-SpReSO) aims to provide one of the most complete optical spectroscopic follow-ups of the far-infrared (FIR) sources detected by the Herschel Space Observatory in the Lockman Hole (LH) field. The optical spectroscopic study of the FIR-selected galaxies supplies valuable information about the relation between fundamental FIR and optical parameters, including extinction, star formation rate, and gas metallicity. In this article, we introduce and provide an in-depth description of the Lockman-SpReSO project and of its early results. Methods. We selected FIR sources from Herschel observations of the central 24 arcmin ×24 arcmin of the LH field with an optical counterpart up to 24.5 RC(AB). The sample comprises 956 Herschel FIR sources, plus 188 additional interesting objects in the field. These are point X-ray sources, cataclysmic variable star candidates, high-velocity halo star candidates, radio sources, very red quasi-stellar objects, and optical counterparts of sub-millimetre galaxies. The faint component of the catalogue (RC(AB) ≥ 20) was observed using the OSIRIS instrument on the 10.4 m Gran Telescopio Canarias in multi-object spectroscopy (MOS) mode. The bright component was observed using two multi-fibre spectrographs: the AF2-WYFFOS at the William Herschel Telescope and the HYDRA instrument at the WYIN telescope. Results. From an input catalogue of 1144 sources, we measured a secure spectroscopic redshift in the range 0.03 ≲ z ≲ 4.96 for 357 sources with at least two identified spectral lines. In addition, for 99 sources that show only one emission or absorption line, a spectroscopic redshift was postulated based on the line and object properties, and photometric redshift. In both cases, properties of emission and absorption lines were measured. Furthermore, to characterize the sample in more depth with determined spectroscopic redshifts, spectral energy distribution (SED) fits were performed using the CIGALE software. The IR luminosity and the stellar mass estimations for the sample are also presented as a preliminary description.

Single molecule localizations of voltage-gated sodium channel NaV1.5 on the surfaces of normal and cancer breast cells.

Voltage-gated sodium channels (VGSCs) are widely expressed in various types of tumor and cancer cells, and NaV1.5 is overexpressed in highly metastatic breast cancer cells. There may be positive relations between the expression levels of NaV1.5 and breast cancer recurrence and metastasis. Herein, NaV1.5 was detected and localized on the surfaces of normal and cancer breast cells by the single molecule recognition imaging (SMRI) mode of atomic force microscopy (AFM). The results reveal that NaV1.5 was irregularly distributed on the surfaces of normal and cancer breast cells. The NaV1.5 has an area percentage of 0.6% and 7.2% on normal and cancer breast cells, respectively, which indicates that there is more NaV1.5 on cancer cells than on normal cells. The specific interaction forces and binding kinetics in the NaV1.5-antibody complex system were investigated with the single molecule force spectroscopy (SMFS) mode of AFM, indicating that the stability of the NaV1.5-antibody on normal breast cells is higher than that on cancer breast cells. All these results will be useful to study the interactions of other ion channel-antibody systems, and will also be useful to understand the role of sodium channels in tumor metastasis and invasion.

The Colors of the Butterfly Wings: Non-Invasive Microanalytical Studies of Hand-Coloring Materials in 19th-Century Daguerreotypes

The public expected color from the early photographic images, yet, daguerreotypes—the first commercially available photographic process—failed to register the natural colors. Daguerreotypists developed several coloring methods to solve this inconvenience after 1840. Scientific analyses of the hand-colored daguerreotypes are limited, and the primary information sources available are manuals and patents. This study aims to contribute to the knowledge of hand-coloring techniques, which impacts conservation practices, mainly the cleaning procedures and complements technical art history investigations. We studied nine colored daguerreotypes with a non-invasive methodology based on three spectroscopic techniques: X-ray fluorescence (XRF), micro-Raman spectroscopy (μ-Raman), and micro-Fourier-transform infrared spectroscopy in reflection mode (μ-rFTIR). The results revealed the different colorants and some of their mixtures employed by the colorist. It also adds information regarding the photographic production of three studios: Désiré François Millet, active in Paris between 1840 and 1868; Antoine Claudet (1797–1867), active in London; and the James E. McClees and Washington Lafayette Germon studio, operative between 1846 and 1855 in Philadelphia, USA. These technical details constitute a helpful comparison to future studies on Daguerreian studios and colored daguerreotypes.

Перспективы реализации наблюдательных программ ГАО РАН в обсерватории Ассы-Тургень

The progress of the observational programs of the Pulkovo observatory involves the transfer of some of them to regions with conducive astroclimatic conditions. The ability of the Assy-Turgen observatory (MPC = 217, h = 2700 m) operated by the Fesenkov Astrophysical Institute (AFIF), (Almaty, Republic of Kazakhstan) causes great interest in this context. The 40-cm telescope MEADE LX200 (Pulkovo Observatory) was transported to this site in 2022. In addition, it became possible to claim part of the observational time of the 1.5-meter AZT-20 telescope, which provides imaging both in the photometric mode in the griz bands and in the spectroscopic mode. The test observations were made with these instruments in August 2022. The present paper discusses the results of these observations and the prospects for further implementation of the Pulkovo observational programs at the Assy-Turgen observatory. It is shown that a positional accuracy better than 50 mas was reached with the 40-cm telescope measurements of the distant satellites of Jupiter and Saturn. Photometric measurements with this telescope allow us to obtain an accuracy of 0.02m. These are competitive values for many types of observations which will be significantly improved after the necessary deep alignment of the telescope optics. The astrometric accuracy of less than 20 mas is reachable with the AZT-20 telescope of this observatory (according to the internal convergence of astrometric measurements observations of the Uranian satellites). Spectral observations of the 61 Cygnus binary system were performed with this instrument in order to develop the method for determining the relative radial velocities. This is necessary to improve the accuracy of determining the orbital parameters of the binary star systems and searching for low-mass components and exoplanets.

Low-loss and temperature stable (1-x)Ba3P2O8-xMg2B2O5 composite ceramics with low sintering temperature

In this study, the Ba3P2O8 and Mg2B2O5 were fabricated by the solid-state reaction method separately, and the (1-x)Ba3P2O8-xMg2B2O5 (x = 0.2–0.4) low-temperature co-fired ceramic (LTCC) materials were obtained in the sintering temperature range of 880–960 °C. The phase compositions, microstructures, elemental compositions, and microwave dielectric properties of the (1-x)Ba3P2O8-xMg2B2O5 composite ceramics were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and TE01δ mode dielectric resonator method, respectively. The results revealed that the Mg2B2O5 phase and Ba3P2O8 phase could coexist well in the (1-x)Ba3P2O8-xMg2B2O5 composite ceramics without formation of any new phases. The abnormal grain growth of Ba3P2O8 grains was inhibited by the addition of Mg2B2O5. In addition, through composition of Ba3P2O8 and Mg2B2O5, the temperature coefficient of resonant frequency (τf) and quality factor (Q×f) were effectively optimized, and the sintering temperature was reduced to 880–960 °C. The optimal performance of 0.8Ba3P2O8-0.2Mg2B2O5 composite ceramic was achieved at a sintering temperature of 920 °C, τf = −1.9 ppm/°C, Q×f = 61,250 GHz, and a low permittivity εr = 10.7. The chemical compatibility test demonstrated that the composite ceramic could coexist well with silver, which indicated that the 0.8Ba3P2O8-0.2Mg2B2O5 composite ceramic is a candidate LTCC material with wide application prospects.

Optical metrology on primary mirror of visible emission line coronagraph on board Aditya-L1 mission

The visible emission line coronagraph (VELC) on board the Aditya-L1 mission is an internally occulted reflective coronagraph. It is capable of simultaneous observations of the solar corona in imaging, spectroscopic, and spectropolarimetric modes very close to the solar limb, to 1.05 R ⊙ (R ⊙ – solar radius). Primary mirror (M1) of the VELC receives the light from both the solar disk and the corona up to 3 R ⊙ . In the VELC, occultation happens at the focus of the M1. Secondary mirror (M2) with a central hole size equal to 1.05 R ⊙ is mounted at the focal plane of M1 and serves the purpose of an internal occulter. To meet the proposed science goals of the payload, M1 surface should be super polished with good imaging characteristics. This results in stringent requirements of the surface figure and microroughness on the mirror surface. M1 is an off-axis parabola, so achieving the demanding requirements is quite challenging. At the same time, testing of M1 after development is crucial for evaluating its performance. This paper provides the details of the optical metrology tests carried out on M1 along with the results obtained and their implications on the performance of the VELC.

Cell-Specific Response of NSIP- and IPF-Derived Fibroblasts to the Modification of the Elasticity, Biological Properties, and 3D Architecture of the Substrate

The fibrotic fibroblasts derived from idiopathic pulmonary fibrosis (IPF) and nonspecific interstitial pneumonia (NSIP) are surrounded by specific environments, characterized by increased stiffness, aberrant extracellular matrix (ECM) composition, and altered lung architecture. The presented research was aimed at investigating the effect of biological, physical, and topographical modification of the substrate on the properties of IPF- and NSIP-derived fibroblasts, and searching for the parameters enabling their identification. Soft and stiff polydimethylsiloxane (PDMS) was chosen for the basic substrates, the properties of which were subsequently tuned. To obtain the biological modification of the substrates, they were covered with ECM proteins, laminin, fibronectin, and collagen. The substrates that mimicked the 3D structure of the lungs were prepared using two approaches, resulting in porous structures that resemble natural lung architecture and honeycomb patterns, typical of IPF tissue. The growth of cells on soft and stiff PDMS covered with proteins, traced using fluorescence microscopy, confirmed an altered behavior of healthy and IPF- and NSIP-derived fibroblasts in response to the modified substrate properties, enabling their identification. In turn, differences in the mechanical properties of healthy and fibrotic fibroblasts, determined using atomic force microscopy working in force spectroscopy mode, as well as their growth on 3D-patterned substrates were not sufficient to discriminate between cell lines.

Low-temperature filamentary plasma for ignition-stabilized combustion

In this work, an experimental study investigating dielectric barrier discharge (DBD) plasma-assisted ignition in methane-air flows at near-atmospheric pressure is presented. Discharges are produced using 10 ns duration high voltage pulses at maximum 3 kHz pulse repetition rate. The goal is to check the feasibility of plasma as an ignition source for ignition-stabilized combustion in a wide range of equivalence ratio, pressure, and flow speed conditions. To that end, four important characteristics are investigated: 1) Ignition dynamics, 2) Minimum number of pulses required for ignition, 3) Plasma energy per pulse, gas temperature, and the effective reduced electric field, and 4) Plasma NOx production. In continuous plasma mode, we observe an elongated flame outside of the discharge region when a methane-air mixture flows through the discharge. The elongated flame is due to repetitively ignited kernels moving downstream with the flow. High-speed intensified imaging is used to explore plasma morphology and ignition dynamics. Plasma has a filamentary nature which is perceived as moving with the flow due to pulse-to-pulse memory effects. For fuel-air mixtures, ignition is followed by kernel expansion and splitting, flame propagation, and consecutive ignition. The time delay between consecutive ignition events is found to be a function of flow speed, because ignition is achieved only when a previously ignited kernel moves out of the plasma region, which happens faster at higher flow speeds. We performed optical emission spectroscopy in burst mode to further characterise plasma and ignition parameters. In air, plasma gas temperature and hence the reduced electric field increases because of pulse-to-pulse effects. By comparing the air plasma temperature with the methane auto-ignition temperature, we conclude that the observed ignition is low-temperature ignition. Parametric studies of NOx measurements suggest that plasma NOx emissions are higher for low flow speeds, high pulse repetition rates, and low-pressure conditions. Overall, our DBD filamentary plasma is found to be a fast, repetitive, and low-temperature ignition source that can be used for ignition-stabilized combustion at near atmospheric conditions.

High-Quality SiO2/O-Terminated Diamond Interface: Band-Gap, Band-Offset and Interfacial Chemistry.

Silicon oxide atomic layer deposition synthesis development over the last few years has open the route to its use as a dielectric within diamond electronics. Its great band-gap makes it a promising material for the fabrication of diamond-metal-oxide field effects transistor gates. Having a sufficiently high barrier both for holes and electrons is mandatory to work in accumulation and inversion regimes without leakage currents, and no other oxide can fulfil this requisite due to the wide diamond band-gap. In this work, the heterojunction of atomic-layer-deposited silicon oxide and (100)-oriented p-type oxygen-terminated diamond is studied using scanning transmission electron microscopy in its energy loss spectroscopy mode and X-ray photoelectron spectroscopy. The amorphous phase of silicon oxide was successfully synthesized with a homogeneous band-gap of 9.4 eV. The interface between the oxide and diamond consisted mainly of single- and double-carbon-oxygen bonds with a low density of interface states and a straddling band setting with a 2.0 eV valence band-offset and 1.9 eV conduction band-offset.

A new approach to time-resolved electrochemical impedance spectroscopy using the Impedance Camera to track fast hydration processes in cement-based materials

In this paper, usefulness and accuracy of the new Impedance Camera working in the time-resolved electrochemical impedance spectroscopy (TR-EIS) mode was demonstrated. Flash setting monophase hydraulic binder material Ca7ZrAl6O18 (C7A3Z), and traditional both Ordinary Portland Cement (OPC) and calcium aluminate cement (CAC)-based alumina spinel castables (CMAS castables) were selected and evaluated. The TR-EIS measurements were conducted in the frequency range from 100 Hz to 1 MHz with 17 point per decade. TR-EIS results were correlated with the hydration heat release determined by calorimetry, and thermal behavior (DSC-TG-EGA(MS)) and phase composition (XRD) of the C7A3Z cement paste. In this work, a new approach to divide the curve of speed impedance changes into few characteristic periods of hydration, different than presented so far as the periods on the heat flow curve obtained by isothermal calorimetry was implemented.

Coupling Sub-nanoliter BDPA Organic Radical Spin Ensembles with YBCO Inverse Anapole Resonators

We report the development and test of planar microwave Inverse Anapole Resonators (IARs) made of superconducting Yttrium Barium Copper Oxide (YBCO) for electron spin resonance spectroscopy on small samples. We first characterize our resonators in zero field and then by carrying out transmission spectroscopy on a diluted $$\alpha ,\gamma $$ -bisdiphenylene- $$\beta $$ -phenylally (BDPA) organic radical spin ensemble in an applied magnetic field. These IARs allow us to carry out electron spin resonance spectroscopy both in continuous-wave and pulsed-wave mode, and to estimate the spin memory time of BDPA. The comparison with the results obtained for the same sample on typical linear coplanar resonators shows an improvement by $$\approx 2\text { - up to}\,3$$ – orders of magnitude in spin sensitivity, with effective sensing volumes below 1 nanoliter. The best sensitivity we achieved is $$S\approx \,10^{7}\,\text {spin}/\sqrt{\mathrm{Hz}}$$ in the pulsed-wave regime. These results compare well with similar experiments reported in the literature.

Scientific study of the pigments and binders used in mural painting from the 16th‐century ce St Mary's Church of Cheriapally, Kottyam, Kerala, and its relevance to conservation

AbstractThe aim of this investigation was to analyze the materials and techniques of the paintings in the 16th‐century ce St Mary's Church, to evaluate deterioration of the paintings, and to propose a suitable conservation treatment for their preservation. The material analysis of ground, pigments, binders, and plasters was undertaken using binocular microscopy, field emission scanning electron microscopy (SEM)–energy dispersive X‐ray spectroscopy, X‐ray fluorescence, Fourier transform infrared spectroscopy in reflectance (KBr) and transmittance mode (attenuated total reflection), thin‐film X‐ray diffraction, and micro‐Raman spectroscopy. Gas chromatography–mass spectrometry (GC‐MS) was used for identifying proteinaceous and lipidic materials extracted from the micro‐samples. The study generated data on two different red and green pigments used as decorative materials. The red pigments were identified as cinnabar along with traces of hematite and clay minerals, and green as malachite and the minor presence of other minerals. SEM analysis identified cotton fiber embedded in the coarse plaster layer that provided better strength to the plaster. Plant oils and proteins found as the binder in the pigment samples suggested that the painting technique was executed as tempera. Lead white and gypsum materials were used in previous restoration works.

IR nanospectroscopy to decipher drug/metal nanoparticle interactions: Towards a better understanding of the spectral signal enhancement and its distribution

In this study, detailed considerations of the interactions appearing at drug/metal nanoparticle interfaces in micro- and nanoscale were performed. Application of the well-known surface-enhanced Raman spectroscopy (SERS) reveals information of how gefitinib, a drug approved in non-small cell lung cancer therapy, connects to the silver and gold nanoparticles (AgNPs and AuNPs, respectively). Such knowledge is important in the context of using the resulting conjugates to increase the effectiveness of cancer therapy. The received data were extended by the nanoscale analysis using atomic force microscopy in combination with the surface-enhanced infrared spectroscopy (AFM-SEIRA) in tapping mode. This allows to better visualize the discussed interaction with ultra-high spatial resolution even reaching the size of the particular nanoparticle (∼15 nm). It should be noted, that the metal nanoparticle mono-layer was enough to enhance the obtained AFM-SEIRA signal, which for the conventional surface-enhanced vibrational techniques would be unattainable. Moreover, since the surface enhancement phenomenon observed in the AFM-SEIRA technique is not fully understood the comparison between this effect and the signal reinforcement in SERS was performed. The drawn conclusions suggest the complementarity of both applied techniques and try to prove the statement that the intensity AFM-SEIRA maps reveal the nature of the drug/metal nanostructure interaction.