Spectrophotometric Determination Of Acetaminophen Research Articles

Application of molecularly imprinted polymer pipette tip micro-solid phase extraction of nalidixic acid and acetaminophen from pills and seawater samples and their determination by spectrophotometry

A molecularly imprinted polymer micro-solid phase extraction in pipette tip was employed for selective extraction and spectrophotometric determination of acetaminophen and nalidixic acid in pills and seawater samples. Parameters affecting microextraction, including pH of the sample, sample volume, type and elution solvent, number of cycles of extraction and desorption and amount of molecular imprinted polymer, were optimized utilizing both response surface methodology and one-variable-at-a-time methods. Four parameters in three levels were applied for response surface methodology experimental design. The proposed method indicated good linear range of 1.0–150.0 µg L−1 for nalidixic acid and 1.0–100.0 µg L−1 for acetaminophen by low detection limits of 0.2 and 0.3 µg L−1, respectively, for nalidixic acid and acetaminophen. Found recoveries were between 88.7 and 99.8% for real samples, with relative standard deviations less than 5.8%. The suggested protocol was successfully utilized to the separation, preconcentration and determination of nalidixic acid and acetaminophen in commercial pills and seawater samples.

Application of AgNPs for Simple and Rapid Spectrophotometric Determination of Acetaminophen and Gentamicin in Real Samples

Application of AgNPs for Simple and Rapid Spectrophotometric Determination of Acetaminophen and Gentamicin in Real Samples

Spectrophotometric Determination of Acetaminophen by Reduction of Molybdenum (VI)

Abstract A new spectrophotometric method for the determination of Acetaminophen (Paracetamol) (ACT) in pure form is described. The procedure is based on the blue colour developed, when the ACT reacts with molybdatophosphoric acid (MPA) in acidified solution under heat treatment. The absorbance of this coloured solution is measured at λ=695 nm and the Lambert-Beer's law is obeyed for 5.0 – 50.0 μg ACT per ml. The measured solution has a molar absorptivity of 1.37×103 1 mol−1cm−1 and a Sandell's sensitivity of about 110 ng cm−2 at 695 nm. The precision and the accuracy are satisfactory, since the over-all relative standard deviation of twenty (20) determinations, the mean standard analytical error and the correlation coefficient were calculated to be 3.8, 0.32 and 0.9999 respectively. The recommended procedure is easily applied to the assay of ACT in pure form. A mean value of t = 0.255 was obtained in a Student's t-test showing the absence of a systematic error in the method, as the corresponding tabulated...

Second Derivative Spectrophotometric Determination of Acetaminophen and Phenacetin in Presence of Their Degradation Products

A rapid and accurate method for determining acetaminophen and phenacetin in presence of their degradation products is presented. Solutions of these drugs in 0.1N HCl were analyzed by measuring their second derivative spectral response at 295 nm where the degradation products do not interfere. The mean percent recoveries for mixtures of acetaminophen and/or phenacetin with the corresponding degradation products were 100.2 +/- 0.6 and 100.6 +/- 1.1, respectively. The method can be used for assessing the stability of the 2 drugs. The proposed method is also applied to the determination of acetaminophen in tablets and syrups.

Spectrophotometric determination of acetaminophen, oxyphenbutazone and salicylamide by nitration and subsequent complexation reactions.

A simple and sensitive spectrophotometric method for the assay of three antipyretic drugs through their nitration and subsequent complexation with an nucleophilic reagent is proposed. The experimental conditions leading to optimum chromagen stability and intensity were studied. The results of the application of the method to the assay of the test compounds in unit doses were compared statistically with those obtained by official methods, and demonstrated good accuracy and precision.

Spectrophotometric Determination of Acetaminophen in Tablets, Sirups, and Suppositories

A differential spectrophotometric method and Glenn's method of orthogonal function are described for the assay of acetaminophen in tablets, sirups, and suppositories. Interference from excipients in the formulations is thereby avoided. Accuracy of the analyses is greater with the proposed methods than with the British Pharmacopoeia method.

Spectrophotometric determination of acetaminophen, salicylamide and codeine phosphate in tablets

An accurate and simple method is proposed for the analysis of a three-component mixture composed of acetaminophen, salicylamide and codeine phosphate, without the necessity for the previous separation of any component. The first two components are determined directly by independent spectrophotometric measurements, based on the pH-induced spectral changes. Codeine phosphate is assayed by the formation of an ion pair with methyl orange. The procedure has been applied successfully to the analysis of known mixtures and commercial tablets.

Spectrophotometric Determination of Acetaminophen and Dichloralantipyrine in Capsules

A rapid method for the routine determination of acetaminophen and dichloralantipyrine in capsules is reported. The determination of acetaminophen is based on the ability of its hydrolytic product, p‐aminophenol, to produce an intensive yellow color with vanillin. The determination of dichloralantipyrine is based on the fact that it, as well as its major metabolite chloral hydrate, produces a blue color with quinaldine ethiodide. No interferences were encountered, and good recovery and precision data were obtained.

Studies on Analysis of Pharmaceutical Preparations. XXIX. Colorimetric Determination of Acetaminophen in Anti-cold Pharmaceutical Preparations

Spectrophotometric Determination of acetaminophen in pharmaceutical preparations is described. Acetaminophen was hydrolysed by refluxing with perchloric acid to produce p-aminophenol which was reacted with p-dimethyl aminocinnamaldehyde in dilute trichloracetic acid solution to give a red color, which showed absorption maximum at 520 nm. Interference by the presence of phenacetin and sulpyrine can be eliminated by their extraction with chloroform in basic medium or extraction with ethyl acetate in acidic medium.

Spectrophotometric Determination of Acetaminophen, Phenylephrine Hydrochloride, Codeine Phosphate, and Pyrilamine Maleate in Tablets or Powder

An ultraviolet spectrophotometric method has been developed for the determination of acetaminophen, phenylephrine hydrochloride, codeine phosphate, and pyrilamine maleate after a partial separation of them by means of column chromatography using alginic acid; codeine phosphate and phenylephrine hydrochloride are both eluted with 0.01 N HCl and determined simultaneously while acetaminophen and pyrilamine maleate are determined separately.