Changes In Spectral Signatures Research Articles

Interpreting chemical enhancements of surface-enhanced Raman scattering

Surface-enhanced Raman scattering (SERS) provides orders of magnitude of enhancements to weak Raman scattering. The improved sensitivity and chemical information conveyed in the spectral signatures make SERS a valuable analysis technique. Most of SERS enhancements come from the electromagnetic enhancement mechanism, and changes in spectral signatures are usually attributed to the chemical enhancement mechanism. As the electromagnetic mechanism has been well studied, we will give an overview of models related to the chemical mechanism, which explain the Raman response in terms of electronic transitions or induced electron densities. In the first class of models based on electronic transitions, chemical enhancements are attributed to changes in transitions of the molecule and new charge transfer transitions. The second class of models relate chemical enhancements to charge flows near the molecule–metal interface by partitioning the induced electron density of the SERS system in real space. Selected examples will be given to illustrate the two classes of models, and connections between the models are demonstrated for prototypical SERS systems.

Exploring plant responses to abiotic stress by contrasting spectral signature changes.

In this study, daily changes over a short period and diurnal progression of spectral reflectance at the leaf level were used to identify spring wheat genotypes (Triticum aestivum L.) susceptible to adverse conditions. Four genotypes were grown in pots experiments under semi-controlled conditions in Chile and Spain. Three treatments were applied: i) control (C), ii) water stress (WS), and iii) combined water and heat shock (WS+T). Spectral reflectance, gas exchange and chlorophyll fluorescence measurements were performed on flag leaves for three consecutive days at anthesis. High canopy temperature ( H CT ) genotypes showed less variability in their mean spectral reflectance signature and chlorophyll fluorescence, which was related to weaker responses to environmental fluctuations. While low canopy temperature ( L CT ) genotypes showed greater variability. The genotypes spectral signature changes, in accordance with environmental fluctuation, were associated with variations in their stomatal conductance under both stress conditions (WS and WS+T); L CT genotypes showed an anisohydric response compared that of H CT , which was isohydric. This approach could be used in breeding programs for screening a large number of genotypes through proximal or remote sensing tools and be a novel but simple way to identify groups of genotypes with contrasting performances.

Snow covered with dust after Chamoli rockslide: inference based on high-resolution satellite data

ABSTRACT The high-resolution multi-temporal PlanetScope image of 7 February 2021 clearly shows the fall of a large part of the Nanda Ghunti glacier (Uttarakhand) down in the base of the valley from a height of about 2000 m. The recorded seismic signals at the local seismic networks, close to the Joshimath station, show the occurrence of the fall of the first glacier block followed by another block which corresponds to the seismic signal recorded the second time. The timings of signals recorded from the seismic station are related to the visual sign of local dust in the valley after the fall of the glacier blocks at 05:01 AM and 05:28 AM UTC on 7 February 2021. In the present paper, we carried out the changes in spectral signatures of PlanetScope imageries and backscattering coefficients from Sentinel-1 synthetic aperture radar (SAR) data at six different locations. Our analysis suggests pronounced changes at all locations based on spectral signatures and backscattering coefficients due to deposition of snow dust due to the fall of the glacier blocks. Changes in surface wetness are evident after the melting of snow due to the deposition of dust in the valley.

Visualizing Polymer Damage Using Hyperspectral Imaging.

Silicone rubbers (SIRs) are common industrial materials which are often used for electrical insulation including weather sheds on non-ceramic insulators (NCIs). While SIRs are typically resilient to outside environments, aging can damage SIRs’ favorable properties such as hydrophobicity and electrical resistance. Detecting SIR aging and damage, however, can be difficult, especially in service. In this study we used hyperspectral imaging (HSI) and previously investigated aging methods as a proof of concept to show how HSI may be used to detect various types of aging damage in different SIR materials. The spectral signature changes in four different SIRs subjected to four different in-service aging environments all occurred between 400––650 nm. Therefore, remote sensing of NCIs using HSI could concentrate on bands below 700 nm to successfully detect in service SIR damage.

Investigation of Salt Stress in Rosemary (Rosmarinus officinalis L.) with the Remote Sensing Technique

Abstract: In the present work, the effect of different salt concentrations on growth and quality of rosemary (Rosmarinus officinalis L.) was investigated using ground-based remote sensing techniques under greenhouse conditions in 2018. The experiment was carried out in a randomized complete block design with three replications and lasted 8 weeks in total. Spectroradiometer measurements were performed on the leaves of rosemary plants to monitor changes in spectral signatures due to salt stress. Spectrophotometer and chlorophyll meter measurements were also taken from the leaves of the plants to investigate the reactions to salt stress at the end of 4th and 8th weeks simultaneously with all other measurements. According to the obtained data, there was a significant difference in the chlorophyll, brightness and colour values of the leaves in response to salt stress, and a difference was observed in the reflective values of the plants in the spectral measurements taken at 4th and 8th weeks. The region with the biggest difference between reflectance values was near-infrared among different salt concentrations applied plants. In the 4th week, the most vivid color (intense green) was obtained in the S3 and S4 applications, (19.80 and 19.40, respectively). However, as the stress application time and the applied salt rate increased, small changes in plant color occurred. Besides, it was concluded that salt treatment increased the NDVI (Normalized Difference Vegetation Index) values of the plants.

Cycling fatigue evaluation using weighted-cumulated methodology

In this paper, the weighted-cumulated methodology designed for the objective muscle fatigue estimation using surface electromyography is improved and extended for cycling applications. An automatic signal burst segmentation is proposed for dynamic electromyography, which is robust to signal amplitude fluctuations and abrupt variation of the experimental protocol parameters. The developed algorithm is integrated into a single instrumental tool. To evaluate the proposed methodology, nine participants performed cycling exercise until muscular failure in three different experimental protocols. In the first protocol, angular velocity was kept constant and exercise power was increased. In the second protocol, power was kept constant and the angular velocity was increased. The third protocol is maintained constant in both power and angular velocity at relatively high values. During the tests, surface electromyography signal was acquired from vastus lateralis. Behavioral characteristics and performance comparison involving the proposed experimental protocols are shown. The interpretations associated with objective metric to estimate muscle fatigue are discussed. The experimental results, obtained using improved weighted-cumulated methodology, are shown to be sensitive enough to distinguish spectral signature changes in the cycling electromyographic signals associated with muscle fatigue. The weighted-cumulated methodology has been shown to be fully applicable in dynamic physical protocols such as cycling. However, the investigations point out that the specification of the experimental protocol is also very important for the results reliability.

Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions.

Two-dimensional fluorescence difference spectroscopy (2-D FDS) was used to determine the unique spectral signatures of zinc oxide (ZnO), magnesium oxide (MgO), and 5% magnesium zinc oxide nanocomposite (5% Mg/ZnO) and was then used to demonstrate the change in spectral signature that occurs when physiologically important proteins, such as angiotensin-converting enzyme (ACE) and ribonuclease A (RNase A), interact with ZnO nanoparticles (NPs). When RNase A is bound to 5% Mg/ZnO, the intensity is quenched, while the intensity is magnified and a significant shift is seen when torula yeast RNA (TYRNA) is bound to RNase A and 5% Mg/ZnO. The intensity of 5% Mg/ZnO is quenched also when thrombin and thrombin aptamer are bound to the nanocomposite. These data indicate that RNA–protein interaction can occur unimpeded on the surface of NPs, which was confirmed by gel electrophoresis, and importantly that the change in fluorescence excitation, emission, and intensity shown by 2-D FDS may indicate specificity of biomolecular interactions.

Lichen biomarkers upon heating: a Raman spectroscopic study with implications for extra-terrestrial exploration

AbstractLithopanspermia Theory has suggested that life was transferred among planets by meteorites and other rocky bodies. If the planet had an atmosphere, this transfer of life had to survive drastic temperature changes in a very short time in its entry or exit. Only organisms able to endure such a temperature range could colonize a planet from outer space. Many experiments are being carried out by NASA and European Space Agency to understand which organisms were able to survive and how. Among the suite of instruments designed for extraplanetary exploration, particularly for Mars surface exploration, a Raman spectrometer was selected with the main objective of looking for life signals. Among all attributes, Raman spectroscopy is able to identify organic and inorganic compounds, either pure or in admixture, without requiring sample manipulation. In this study, we used Raman spectroscopy to examine the lichen Squamarina lentigera biomarkers. We analyse spectral signature changes after sample heating under different experimental situations, such as (a) laser, (b) analysis accumulations over the same spot and (c) environmental temperature increase. Our goal is to evaluate the capability of Raman spectroscopy to identify unambiguously life markers even if heating has induced spectral changes, reflecting biomolecular transformations. Usnic acid, chlorophyll, carotene and calcium oxalates were identified by the Raman spectra. From our experiments, we have seen that usnic acid, carotene and calcium oxalates (the last two have been suggested to be good biomarkers) respond in a different way to environmental heating. Our main conclusion is that despite their abundance in nature or their inorganic composition the resistance to heat makes some molecules more suitable than others as biomarkers.

FTIR spectroscopic imaging and mapping with correcting lenses for studies of biological cells and tissues.

Histopathology of tissue samples is used to determine the progression of cancer usually by staining and visual analysis. It is recognised that disease progression from healthy tissue to cancerous is accompanied by spectral signature changes in the mid-infrared range. In this work, FTIR spectroscopic imaging in transmission mode using a focal plane array (96 × 96 pixels) has been applied to the characterisation of Barrett's oesophageal adenocarcinoma. To correct optical aberrations, infrared transparent lenses were used of the same material (CaF2) as the slide on which biopsies were fixed. The lenses acted as an immersion objective, reducing scattering and improving spatial resolution. A novel mapping approach using a sliding lens is presented where spectral images obtained with added lenses are stitched together such that the dataset contained a representative section of the oesophageal tissue. Images were also acquired in transmission mode using high-magnification optics for enhanced spatial resolution, as well as with a germanium micro-ATR objective. The reduction of scattering was assessed using k-means clustering. The same tissue section map, which contained a region of high grade dysplasia, was analysed using hierarchical clustering analysis. A reduction of the trough at 1077 cm(-1) in the second derivative spectra was identified as an indicator of high grade dysplasia. In addition, the spatial resolution obtained with the lens using high-magnification optics was assessed by measurements of a sharp interface of polymer laminate, which was also compared with that achieved with micro ATR-FTIR imaging. In transmission mode using the lens, it was determined to be 8.5 μm and using micro-ATR imaging, the resolution was 3 μm for the band at a wavelength of ca. 3 μm. The spatial resolution was also assessed with and without the added lens, in normal and high-magnification modes using a USAF target. Spectroscopic images of cells in transmission mode using two lenses are also presented, which are necessary for correcting chromatic aberration and refraction in both the condenser and objective. The use of lenses is shown to be necessary for obtaining high-quality spectroscopic images of cells in transmission mode and proves the applicability of the pseudo hemisphere approach for this and other microfluidic systems.

Losing control under ketamine: suppressed cortico-hippocampal drive following acute ketamine in rats.

Systemic doses of the psychotomimetic ketamine alter the spectral characteristics of hippocampal and prefrontal cortical network activity. Using dynamic causal modeling (DCM) of cross-spectral densities, we quantify the putative synaptic mechanisms underlying ketamine effects in terms of changes in directed, effective connectivity between dorsal hippocampus and medial prefrontal (dCA1-mPFC) cortex of freely moving rats. We parameterize dose-dependent changes in spectral signatures of dCA1-mPFC local field potential recordings, using neural mass models of glutamatergic and GABAergic circuits. Optimizing DCMs of theta and gamma frequency range responses, model comparisons suggest that both enhanced gamma and depressed theta power result from a reduction in top-down connectivity from mPFC to the hippocampus, mediated by postsynaptic NMDA receptors (NMDARs). This is accompanied by an alteration in the bottom-up pathway from dCA1 to mPFC, which exhibits a distinct asymmetry: here, feed-forward drive at AMPA receptors increases in the presence of decreased NMDAR-mediated inputs. Setting these findings in the context of predictive coding suggests that NMDAR antagonism by ketamine in recurrent hierarchical networks may result in the failure of top-down connections from higher cortical regions to signal predictions to lower regions in the hierarchy, which consequently fail to respond consistently to errors. Given that NMDAR dysfunction has a central role in pathophysiological theories of schizophrenia and that theta and gamma rhythm abnormalities are evident in schizophrenic patients, the approach followed here may furnish a framework for the study of aberrant hierarchical message passing (of prediction errors) in schizophrenia-and the false perceptual inferences that ensue.

Lateral heterogeneity in the convecting mantle

Thermally induced lateral heterogeneity in the earth's mantle has been studied in terms of idealized two‐dimensional, constant viscosity, numerical models of mantle convection. Steady model solutions of the temperature and velocity fields were analyzed with respect to both the spatial and spectral signatures of their inherent lateral heterogeneity. By examining the changes in spectral signature produced by varying the Rayleigh number, the degree of internal heating, and the depth in the convection cell, it has been possible to identify those features of the spectra of lateral heterogeneity which are most indicative of these variations. Particular attention has been paid to the spectral characteristics of thermal boundary layers within the convecting layer. The spectral analysis of known temperature fields represents the forward problem corresponding to the inverse problem of inferring the thermal structure of the mantle from the spectral components of its lateral heterogeneity as determined by seismic tomography. The forward problem presented here illustrates a new approach in convection modeling which may ultimately provide important diagnostic criteria for the interpretation of recent tomographic data.

A crop moisture stress index for large areas and its application in the prediction of spring wheat phenology

Crop phenology models are used in remote sensing programs for interpreting changes in spectral signatures during the growing season, in yield modeling, and in early warning of episodic events that affect crop production over large areas. An agrometeorological crop phenology model for spring wheat that incorporates the photothermal concept suggested in the Robertson crop calendar model is presented and modified to incorporate a crop moisture stress index as an additional model parameter. The crop stress index parameter is derived from comparison of crop water use under unlimited water conditions. The index may be used in other applications such as yield predictions. The influence of moisture stress on phenological development was derived from the literature. Development in general is retarded when moisture stress occurs early in the vegetative phase. Moisture deficit conditions after flowering hasten senescence, possibly through an increase in leaf temperature. Validity of the model was tested in the 1980 spring wheat areas in north Dakota, South Dakota, Minnesota and Montana. The model is flexible to incorporate remotely sensed multispectral data as inputs.