Spectral Scanning Research Articles

Strain gauge based on n-pairs of chirped fiber Bragg gratings

Strain gauges based on fiber Bragg gratings are not only a well-developed field of research, but also gain a wide market due to their high sensitivity, response speed, immunity to electromagnetic radiation and flexible characteristics. In this work, we studied the strain gauge sensor based on n-pairs of chirped fiber Bragg gratings. These structures are inscribed at the same Bragg resonance wavelength within each individual pair. One grating is sensitive to applied load, the other is in the free position. Under mechanical impact, load-sensitive grating shifts to the region of long wavelengths. In this regard, the total reflection spectrum becomes wider and the reflected optical power increases. Bragg gratings are inscribed with reflection coefficients close to 100%. The experimental results to identify the dependencies between the applied mechanical stress and the reflected optical power are obtained. The proposed implementation of the sensor does not require spectral scanning of the gratings, which greatly simplifies its operation.

Ultrafast parallel single-pixel LiDAR with all-optical spectro-temporal encoding

Light detection and ranging (LiDAR) with scanning beam has been widely used in autonomous driving and large-scale manufacturing, where the fast-changing scene is of great interest. However, the acquisition rate of current LiDAR is limited by beam steering speed and the round-trip delay, hindering the development of video-rate LiDAR. Here, we uniquely combine all-optical encoders with wavelength-division multiplexing devices to implement spectro-temporal encoding on the illumination light. Parallel detection and fast spectral scanning can be achieved with only one laser and one single photodetector. Our result shows the fastest single-pixel LiDAR to date, and the detection speed can be multiplied with scalability. We demonstrate 3.56-fold improvement of speed for a maximum of 75 m detection range compared with a serial LiDAR. A record acquisition rate of 21.38 MHz for a maximum of 25 m detection range is obtained. This approach has the potential to break the limitation on current ultrafast single-pixel LiDAR and opens a new paradigm for ultrafast-frame-rate imaging systems.

Rational design and synthesis of a novel BODIPY-based probe for selective imaging of tau tangles in human iPSC-derived cortical neurons

Numerous studies have shown a strong correlation between the number of neurofibrillary tangles of the tau protein and Alzheimer's disease progression, making the quantitative detection of tau very promising from a clinical point of view. However, the lack of highly reliable fluorescent probes for selective imaging of tau neurofibrillary tangles is a major challenge due to sharing similar β–sheet motifs with homologous Amyloid-β fibrils. In the current work, we describe the rational design and the in silico evaluation of a small-size focused library of fluorescent probes, consisting of a BODIPY core (electron acceptor) featuring highly conjugated systems (electron donor) with a length in the range 13–19 Å at C3. Among the most promising probes in terms of binding mode, theoretical affinity and polarity, BT1 has been synthesized and tested in vitro onto human induced pluripotent stem cells derived neuronal cell cultures. The probe showed excellent photophysical properties and high selectivity allowing in vitro imaging of hyperphosphorylated tau protein filaments with minimal background noise. Our findings offer new insight into the structure-activity relationship of this class of tau selective fluorophores, paving the way for boosting tau tangle detection in patients possibly through retinal spectral scans.

Quantitative analysis of optical coherence tomography imaging in patients with different severities of hydroxychloroquine toxicity

PurposeTo determine the diagnostic validity of quantitative measures derived from optical coherence tomography (OCT) images in their ability to discriminate between cohorts of eyes unaffected by hydroxychloroquine (HCQ) and those...

Mass production-enabled computational spectrometers based on multilayer thin films

Multilayer thin film (MTF) filter arrays for computational spectroscopy are fabricated using stencil lithography. The MTF filter array is a 6 × 6 square grid, and 169 identical arrays are fabricated on a single wafer. A computational spectrometer is formed by attaching the MTF filter array on a complementary metal–oxide–semiconductor (CMOS) image sensor. With a single exposure, 36 unique intensities of incident light are collected. The spectrum of the incident light is recovered using collected intensities and numerical optimization techniques. Varied light sources in the wavelength range of 500 to 849 nm are recovered with a spacing of 1 nm. The reconstructed spectra are a good match with the reference spectra, measured by a grating-based spectrometer. We also demonstrate computational pinhole spectral imaging using the MTF filter array. Adapting a spectral scanning method, we collect 36 monochromatic filtered images and reconstructed 350 monochromatic images in the wavelength range of 500 to 849 nm, with a spacing of 1 nm. These computational spectrometers could be useful for various applications that require compact size, high resolution, and wide working range.

Spectroscopic confirmation of a gravitationally lensed Lyman-break galaxy at z[C ii] = 6.827 using NOEMA

ABSTRACT We present the spectroscopic confirmation of the brightest known gravitationally lensed Lyman-break galaxy in the Epoch of Reionization (EoR), A1703-zD1, through the detection of [C ii] 158 $\mu$m at a redshift of z = 6.8269 ± 0.0004. This source was selected behind the strong lensing cluster Abell 1703, with an intrinsic luminosity and a very blue Spitzer/Infrared Array Camera (IRAC) [3.6]–[4.5] colour, implying high equivalent width line emission of [O iii] + Hβ. [C ii] is reliably detected at 6.1σ cospatial with the rest-frame ultraviolet (UV) counterpart, showing similar spatial extent. Correcting for the lensing magnification, the [C ii] luminosity in A1703-zD1 is broadly consistent with the local $L_{\rm [C\, {\small II}]}$–star formation rate (SFR) relation. We find a clear velocity gradient of 103 ± 22 km $\rm s^{-1}$ across the source that possibly indicates rotation or an ongoing merger. We furthermore present spectral scans with no detected [C ii] above 4.6σ in two unlensed Lyman-break galaxies in the Extended Groth Strip (EGS)-Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey (CANDELS) field at z ∼ 6.6–6.9. This is the first time that the Northern Extended Millimeter Array (NOEMA) has been successfully used to observe [C ii] in a ‘normal’ star-forming galaxy at z > 6, and our results demonstrate its capability to complement the Atacama Large Millimeter/submillimeter Array (ALMA) in confirming galaxies in the EoR.

Discovery of Kinetic Effect in a Valence Tautomeric Cobalt-Dioxolene Complex.

Two isostructural valence tautomeric (VT) complexes with different critical temperatures were prepared and fully investigated through a series of magnetic, structural, spectral, and differential scanning calorimetry evidence. The kinetic effect in the VT complex was observed for the first time through scan-rate-dependent studies and further validated by annealing tests.

NOEMA confirmation of an optically dark ALMA–AzTEC submillimetre galaxy at z = 5.24

We have obtained deep 1 and 3 mm spectral-line scans towards a candidate z ≳ 5 ALMA-identified AzTEC submillimetre galaxy (SMG) in the Subaru/XMM-Newton Deep Field (or UKIDSS UDS), ASXDF1100.053.1, using the NOrthern Extended Millimeter Array (NOEMA), aiming to obtain its spectroscopic redshift. ASXDF1100.053.1 is an unlensed optically dark millimetre-bright SMG with S1100 μm = 3.5 mJy and KAB > 25.7 (2σ), which was expected to lie at z = 5–7 based on its radio–submillimetre photometric redshift. Our NOEMA spectral scan detected line emission due to 12CO(J = 5–4) and (J = 6–5), providing a robust spectroscopic redshift, zCO = 5.2383 ± 0.0005. Energy-coupled spectral energy distribution modelling from optical to radio wavelengths indicates an infrared luminosity LIR = 8.3−1.4+1.5 × 1012 L⊙, a star formation rate SFR = 630−380+260 M⊙ yr−1, a dust mass Md = 4.4−0.3+0.4 × 108 M⊙, a stellar mass Mstellar = 3.5−1.4+3.6 × 1011 M⊙, and a dust temperature Td = 37.4−1.8+2.3 K. The CO luminosity allows us to estimate a gas mass Mgas = 3.1 ± 0.3 × 1010 M⊙, suggesting a gas-to-dust mass ratio of around 70, fairly typical for z ∼ 2 SMGs. ASXDF1100.053.1 has ALMA continuum size Re = 1.0−0.1+0.2 kpc, so its surface infrared luminosity density ΣIR is 1.2−0.2+0.1 × 1012 L⊙ kpc−2. These physical properties indicate that ASXDF1100.053.1 is a massive dusty star-forming galaxy with an unusually compact starburst. It lies close to the star-forming main sequence at z ∼ 5, with low Mgas/Mstellar = 0.09, SFR/SFRMS(RSB) = 0.6, and a gas-depletion time τdep of ≈50 Myr, modulo assumptions about the stellar initial mass function in such objects. ASXDF1100.053.1 has extreme values of Mgas/Mstellar, RSB, and τdep compared to SMGs at z ∼ 2–4, and those of ASXDF1100.053.1 are the smallest among SMGs at z > 5. ASXDF1100.053.1 is likely a late-stage dusty starburst prior to passivisation. The number of z = 5.1–5.3 unlensed SMGs now suggests a number density dN/dz = 30.4 ± 19.0 deg−2, barely consistent with the latest cosmological simulations.

Enzymatic Biodegradation, Kinetic study, and Detoxification of Reactive Red-195 by Halomonas meridiana Isolated from Marine Sediments of Andaman Sea, India

Azo dyes are a significant class of hazardous chemicals that are extensively utilized in diverse industries. Industries that manufacture and consume reactive azo dyes generate hyper-saline wastewater. The ability of halotolerant bacteria to thrive under extreme environmental conditions thus makes them a potential candidate for reactive azo dye degradation. An efficient halotolerant bacterium (isolate SAIBP-6) with the capability to degrade 87.15% of azo dye Reactive Red 195 (RR-195) was isolated from sea sediment and identified as Halomonas meridiana SAIBP-6. Strain SAIBP-6 maintained potential decolorization under a wide range of environmental conditions viz. 35–45 ℃ temperature, 50–450 mg/L RR-195, pH 7–9, and 50–150 g/L NaCl. However, maximum decolorization occurred at 40 ℃, 200 mg/L RR-195 dye, pH 9, and 50 g/L NaCl, under static conditions. Tyrosinase and azoreductase were responsible for dye degradation. The reaction catalyzed by these enzymes followed zero-order kinetics. The maximum velocity (Vmax) of the enzymatic reaction was 4.221 mg/(L.h) and the Michaelis constant (Km) was 517.982 mg/L. Strain SAIBP-6 also efficiently decolorized Reactive Black-5 and Reactive Yellow-160 dye. The biodegradation process was further studied with the help of UV-Vis spectral scan, ultra-high performance liquid chromatography (UPLC), fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) analysis. Finally, cytogenotoxicity assay conducted with the meristematic root tip cells of Allium cepa and phytotoxicity assay conducted with the seeds of Vigna mungo led to the inference that strain SAIBP-6 significantly reduced the toxicity of RR-195 after biodegradation.

High-Resolution Simulation of Externally Injected Lasers Revealing a Large Regime of Noise-Induced Chaos

We present comprehensive numerically simulated scans of the spectral evolution of the output from a single-mode semiconductor laser diode undergoing external light injection. The spectral scans are helpful to understand the different regimes of operation as well as the system evolution between each state: i.e., locked state, four-wave mixing, pulsations, chaos. We find that, when under strong injection, when the injected power equals about half of the laser power, two distinct regions of chaotic behaviour are observed. One of the chaotic regions arises due to the usual period-doubling route to chaos; the other chaotic region is a blurring of what would be higher-order period pulsations whose periodicity is broken by spontaneous emission and the laser spectrum is chaotic. Eliminating spontaneous emission in our simulations confirms the latter chaotic region becomes a region with higher-order pulsations.

Single-shot multiwavelength digital holography using Bragg diffraction of light by several ultrasound waves [Invited

We report on wavelength-multiplexed digital holographic imaging based on simultaneous Bragg diffraction of wideband light by several ultrasound waves of different frequencies in crystalline media. This technique is easy to implement, avoids spectral scanning, and is applicable in various digital holography schemes. It also enables single-shot acquisition of a few spectral fringe patterns by a single monochrome sensor and wavelength demultiplexing of the resulting interferogram. We have assembled a Mach-Zehnder interferometer with an acousto-optical tunable filter operating in the multifrequency mode and have validated the proposed technique by dual-, three-, and four-wavelength quantitative phase imaging of the test samples.

A Qualitative Examination of the Iron Boomerang and Trends in Spectral Metrics across Iron Ore Deposits in Western Australia

There are two major types of iron ore deposits in the Pilbara Province of Western Australia—banded iron formation (BIF)-hosted iron ore deposits and bedded iron deposits (BID), respectively, named martite–goethite and martite–microplaty hematite and the channel iron deposits (CID). These deposits consist mainly of iron oxides such as magnetite, hematite and goethite; the latter have been subdivided into vitreous and ochreous goethite. Combining spectral scanning of diamond drill core, drill chips and pulps collected from these deposits provides a rapid and relatively inexpensive means of assessing the potential mineral makeup within a deposit to make informed qualitative decisions. Additionally, the full width half maximum (FWHM) of the 900 nm 6A1à4T1 crystal field absorption feature within the goethite-dominated region is shown to be related to the type of goethite, namely ochreous and vitreous. The assessment capabilities of the combined metrics are presented in a visual format named as the iron boomerang because of its distinctive manifold. This provides the identification of at least two spectral endmembers comprised of hematite and vitreous goethite, the identification of samples that are moving from a pure hematite to mixed hematite/goethite and lastly into a goethite-dominant-driven regime.

Solid-state FMCW LiDAR with in-fiber beam scanner.

The beam scanner is a predominant part in the light detection and ranging (LiDAR) system to achieve three-dimensional (3D) imaging. The solid-state beam-steering device has emerged as a promising candidate technology for a beam scanner with the advantages of robustness, stability, and high scanning speed. Here we propose a frequency modulated continuous wave (FMCW) LiDAR system with an in-fiber solid-state beam scanner. A 45° tilted fiber grating (TFG) is first employed to achieve in-fiber solid-state spectral scanning in the LiDAR system. A maximum output efficiency of 93.7% is achieved with proper polarization control. A single-mode fiber is then used to fabricate a 2-cm 45° TFG, which significantly reduces the size and the cost of the beam scanner in the LiDAR system. We experimentally realize 3D imaging of targets placed at a distance of 1.2 m based on our proposed LiDAR system. In addition, the system can achieve a detection distance of 6 m with a ranging precision of 24 mm.

A Robust Microbead Occlusion Model of Glaucoma for the Common Marmoset.

PurposeTo establish a robust experimental model of glaucoma in the common marmoset (Callithrix jacchus), a New World primate, using an intracameral microbead injection technique.MethodsElevated intraocular pressure (IOP) was induced by an injection of polystyrene microbeads. Morphologic changes in the retina and optic nerve of glaucomatous eyes were assessed and electroretinogram (ERG) recordings were performed to evaluate functional changes.ResultsMicrobead injections induced a sustained IOP elevation for at least 10 weeks in a reproducible manner. At the end of the 10-week experimental period, there was significant loss of retinal ganglion cells (RGCs) in all quadrants and eccentricities, although it was more prominent in the mid-peripheral and peripheral regions. This was consistent with a thinning of the Retinal nerve fiber layer (RNFL) seen in spectral domain optical coherence tomography scans. Surviving RGCs showed marked changes in morphology, including somatic shrinkage and dendritic atrophy. Retinas also showed significant gliosis. The amplitude of the ERG photopic negative response, with subsequent a- and b-wave changes, was reduced in glaucomatous eyes. The optic nerve of glaucomatous eyes showed expanded cupping, disorganization of the astrocytic matrix, axonal loss, and gliosis.ConclusionsWe developed a robust and reproducible model of glaucoma in the marmoset. The model exhibits both structural and functional alterations of retina and optic nerve characteristic of glaucoma in humans and animal models.Translational RelevanceThe glaucoma model in the marmoset described here forms a robust method to study the disease etiology, progression, and potential therapies in a nonhuman primate, allowing for more effective translation of animal data to humans.

DISCRIMINATION OF EYES WITH PRIMARY OPEN-ANGLE GLAUCOMA FROM NORMAL USING MACULAR GC-IPL THICKNESS

Objective: Primary open-angle glaucoma (POAG), a neuro-ophthalmological disease, is a condition of slowly progressive loss of retinal ganglion cells which are evident as characteristic optic nerve head vicissitudes and visual field defects, only after death of more than 40% of cells. This huge loss when witnessed is started to treat only if the patient approaches an ophthalmologist with a significant complaint, leading to optic nerve head examination, which means being an asymptomatic disease, it is usually left undiagnosed until advanced. An additional system of diagnosis of the disease earlier in the stage can aid reduce the burden of the blindness of POAG. Methods: Among a total of 62 eyes of 31 subjects, 40 eyes of 20 POAG subjects, and 22 eyes of 11 age-matched healthy subjects were recruited. All of them underwent Spectral Domain Optical Coherence Tomography macular and optic disk scans to note macular ganglion cell–inner plexiform layer (mGCIPL) and retinal nerve fiber layer (RNFL) thicknesses. Statistical analysis was did using an unpaired t-test and calculating a two-tailed p value, in which the significance was indicated by p<0.05. Results: Similar to peripapillary RNFL thickness, the mGCIPL parameters, that is, minimum GCIPL thickness and GCIPL thickness in all sectors decreased significantly in POAG eyes to be able to discriminate them from normal. mGCIPL and RNFL thickness decreased as the severity of glaucoma increased. Conclusion: The minimum GCIPL thickness and that in all sectors can discriminate POAG eyes from healthy eyes significantly and has comparable performance to that of peripapillary RNFL thickness.

Searching for high-z DSFGs with NIKA2 and NOEMA

As the possible progenitors of passive galaxies at z=2-3, dusty starforming galaxies (DSFGs) at z>4 provide a unique perspective to study the formation, assembly, and early quenching of massive galaxies in the early Universe. The extreme obscuration in optical-IR makes (sub)mm spectral scans the most universal and unbiased way to confirm/exclude the high-z nature of candidate dusty star-forming galaxies. We present here the status of the NIKA2 Cosmological Legacy Survey (N2CLS), which is the deepest wide-area singledish survey in the millimeter searching for high-z DSFGs. We also introduce a joint-analysis method to effciently search for the spectroscopic redshift of high-z DSFGs with noisy spectra and photometric data and present its success in identifying the redshift of DSFGs found in NIKA2 science verification data.

A Prototype of a Large Tunable Fabry–Pérot Interferometer for Solar Spectroscopy

Large Fabry–Pérot Interferometers (FPIs) are used in a variety of astronomical instrumentation, including spectro-polarimeters for 4 m class solar telescopes. In this work we comprehensively characterize the cavity of a prototype 150 mm FPI, sporting a novel, fully symmetric design. Of particular interest, we define a new method to properly assess the gravity effects on the interferometer’s cavity when the system is used in either the vertical or horizontal configuration, both typical of solar observations. We show that the symmetric design very effectively limits the combined effects of pre-load and gravity forces to only a few nm over a 120 mm diameter illuminated surface, with gravity contributing ∼2 nm peak-to-valley (∼0.3 nm rms) in either configuration. We confirm a variation of the tilt between the plates of the interferometer during the spectral scan, which can be mitigated with appropriate corrections to the spacing commands. Finally, we show that the dynamical response of the new system fully satisfies typical operational scenarios. We conclude that large, fully symmetric FPIs can be safely used within solar instrumentation in both, horizontal and vertical position, with the latter better suited to limiting the overall volume occupied by such an instrument.

Measurement of Myopia and Normal Human Choroidal Thickness Using Spectral Domain Optical Coherence Tomography

Myopia is a common ophthalmic deficiency. The structure and function of choroid layer is assumed to be associated with myopia. In this study, a laboratory developed spectral domain optical coherence tomography scanning system is used to image human eyes. The axial resolution of the system is about 7 μm, and the acquisition rate is 100 kHz. Firstly, a cross-sectional image was acquired by averaging 100 images from imaging posterior segment of each eye. The choroid thickness was measured by 11 discrete points. The average thickness of normal human eyes was (0.296 ± 0.126) mm, whereas the average choroid thickness of myopic eyes was (0.220 ± 0.095) mm. Afterwards, the T test is used to calculate the data statistically. The analysis of the final result is based on the average thickness measured and the thickness of each measuring point. There was a significant difference in choroid thickness between myopia and normal eyes (P value < 0.01), which indicates that the choroid thickness of myopia was significantly thinner than that of normal eyes. Besides, there are findings that the choroidal thickness in nasal side is thinner than that in the fovea and temporal side in each eye. The choroidal thickness on temporal side in myopia eye has the most significant difference comparing with that in normal eye. The comprehensive evaluation of myopia and normal choroidal thickness using spectral domain optical coherence tomography may provide an important reference for the development of medical methods for diagnosis and treatment of myopia.

BIOSURFACTANT PRODUCTION BY ENDOPHYTIC ISOLATES FROM RHIZOMES OF ZINGIBER ZERUMBET

Biosurfactants (BS) or green surfactants are amphiphilic compounds that enhance the bioavailability of hydrophobic organic compounds and also exhibit anti-phytopathogenic properties. BSproducing isolates thus find applications in soil remediation and development of biocontrol agents. Endophytes that confer several benefits to host plant constitute a good resource for prospecting BSproducing isolates. The present study screened the Z. zerumbet endophytes identified in earlier studies for BS production by hydrocarbon overlay agar method, CTAB- Methylene blue agar assay and determining Emulsification Index (EI24). EI24 was observed as the most desirable parameter to determine BS production compared to hydrocarbon overlay agar and CTAB- methylene blue agar plate assay. Isolate ZzADK4 (Bacillus spp) showed highest emulsification activity (58%) determined as EI% with kerosene. UV-vis spectral scans identified the endophytes as producing LP class of BS with absorbance maxima spanning 207- 216 nm. LP class of BS producing endophytic isolates identified in the present study constitute potential solutions with applications in soil remediation and the development of biocontrol agents (BCAs).

Characterization of Commercially Available Turmeric for Use in Pharmaceutical Products and Food Supplements

We addressed some physicochemical and biological differences in commercial turmeric, acquired from both handling pharmacies and stores selling natural products for quality assessment. For this, it was determined the melting point, antioxidant activity by 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH), antimicrobial activity via the agar disk-diffusion assay, metallic contaminants by X-ray fluorescence with energy dispersion (EDXRF), spectral behaviors by Fourier transform infrared (FTIR) and ultraviolet-visible (UV-Vis) and determination of curcumin concentration by UV-Vis. The results obtained of the several commercial turmeric samples were not homogeneous, showing in fact significant differences regarding melting points, UV-Vis spectral scan profiles, and FTIR spectra, presenting toxic metals and quite low curcumin contents. This sheds light on the urge to implement adequate quality control in this type of raw material for human safe use of curcumin. Particularly, contamination with heavy metals, such as mercury, is a serious health problem, due to its high toxicity and accumulative power in the body.