An arrangement in which a diffuser may be utilised to provide full field speckle illumination in a fluorescence microscope is described. The design requires that the conventional image of the speckle-illuminated object and a separate image of the speckle pattern are recorded or processed in real time. An alternative design could utilise a light valve. An analysis of the performance of the proposed microscope is given which shows that with adequate averaging the microscope response is equivalent to that of a scanning confocal arrangement. A computer simulation is used to demonstrate the enhanced lateral resolution and depth discrimination and also to assess the amount of averaging required for high fidelity imaging.
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