Nematode specimens collected from the faeces of the Indian one horned Rhinoceros (Rhinoceros unicornis) in the Alipore Zoological Garden, Calcutta, India are diagnosed as a new species under genus Probstmayria and named as Probstmayria bengalensis n. sp. The new species is distinguished from the other valid species of the genus in the presence of 3 pairs of postanal papillae and unequal, similar spicules (42.9–49.5 μm for left and 26.4–29.7 μm for the right spicule), with a spicular ratio of 1:1.62–1.66 and a small gubernaculum (13.2–16.5 μm long), that bents towards dorsal surface.