Preserved Specimens Research Articles

ILLUSTRATED TYPE CATALOGUE OF THE “LOST” HERPETOLOGICAL COLLECTIONS OF MUSEU DO DUNDO, ANGOLA

The herpetological collections of the Museu do Dundo in Lunda Norte Province, northeast Angola, are among the most important in southern Africa and represent one of the largest collections of Angolan amphibians and reptiles in the world. The collection comprises more than 2,750 preserved specimens, including type specimens of taxa described by Raymond F. Laurent during the 1950s and 1960s, when he was affiliated with the Musée royal de l'Afrique centrale (RMCA) in Tervuren, Belgium, and the Museum of Comparative Zoology (MCZ) at Harvard University, Cambridge, Massachusetts, where portions of these type series were also deposited. We provide details for all type specimens and summarize the history and taxonomy for each species represented in the type collection. The collections contain type specimens of 28 amphibian and reptile species, including seven snakes: Typhlops praeocularis lundensis, Dispholidus typus punctatus, Lycodonomorphus subtaeniatus, Lycophidion hellmichi, Gonionotophis brussauxi prigoginei, Prosymna ambígua brevis, and Elapsoidea decosteri huilensis; 13 lizards: Rhoptropus boultoni montanus, Rhoptropus taeniostictus, Hemidactylus nzingae, Lygodactylus tchokwe, Cordylus vittifer machadoi, Chamaesaura anguina oligopholis, Gerrhosaurus bulsi, Nucras scalaris, Ichnotropis bivittata pallida, Mabuya bayonii huilensis, Mabuya ivensii septemlineata, Trachylepis raymondlaurenti, and Eumecia anchietae major; one amphisbaenian: Monopeltis vanderysti vilhenai; and seven frogs: Hyperolius machadoi, Hyperolius marmoratus alborufus, Hyperolius vilhenai, Ptychadena grandisonae, Ptychadena loveridgei, Ptychadena perplicata, and Ptychadena upembae machadoi. The type specimens of the snake Xenocalamus bicolor machadoi were not found in the collections. A brief history of the museum and remarks on the overall herpetological collections are also provided. We also note additional information about the related type material of these taxa at the MCZ.

The Open-Specimen Movement

AbstractThe open-science movement seeks to increase transparency, reproducibility, and access to scientific data. As primary data, preserved biological specimens represent records of global biodiversity critical to research, conservation, national security, and public health. However, a recent decrease in specimen preservation in public biorepositories is a major barrier to open biological science. As such, there is an urgent need for a cultural shift in the life sciences that normalizes specimen deposition in museum collections. Museums embody an open-science ethos and provide long-term research infrastructure through curation, data management and security, and community-wide access to samples and data, thereby ensuring scientific reproducibility and extension. We propose that a paradigm shift from specimen ownership to specimen stewardship can be achieved through increased open-data requirements among scientific journals and institutional requirements for specimen deposition by funding and permitting agencies, and through explicit integration of specimens into existing data management plan guidelines and annual reporting.

A new species of the hermit crab genus Cancellus H. Milne Edwards, 1836 from a mesophotic deep bank in the northwestern Gulf of Mexico (Crustacea: Decapoda: Diogenidae).

Recent sampling on mesophotic deep banks in the northwestern Gulf of Mexico has produced a previously undescribed hermit crab assignable to the genus Cancellus H. Milne Edwards, 1836. Members of the genus are most often found to occupy cavities of eroded coral, siliceous sponges, porous calcareous rock fragments, algal concretions, or worm tubes as shelters. The present specimen was found loose as by-catch in a dredged rhodolith sample taken for algal life history studies. In situ, it likely occupied a cavity within one of the collected calcareous rhodoliths or small sponges in the by-catch. While our description is based on a single female specimen, the holotype is fully mature and intact, and it was solidly frozen in seawater until its coloration could be photographically documented and tissues extracted for sequencing. In comparison to the three other known western Atlantic species, the frontal rim of the carapace shield in the new species is continuous between the blunt lateral teeth as in C. ornatus Benedict, 1901 and C. viridis Mayo, 1873, and thus distinct from the subdivided front found in C. spongicola Benedict, 1901. The rim itself is somewhat flattened as in C. ornatus rather than inflated as in C. viridis. However, each of the ocular scales bears a pair of spines at the tip, as in C. viridis. The lower palms of the chelipeds, while distinctly rugose, do not have a separated patch of stridulating ridges comparable to those reported for C. spongicola. The yellow-orange to deep-orange pigmentation of the color pattern differs from fresh coloration in both C. ornatus and C. viridis, but that of C. spongicola is unknown for other than preserved specimens. Description of the single available specimen is in this case justified by the low likelihood for timely acquiring of additional samples from the type locality or adjacent habitats, most of which are deep banks warranting protection under pending habitat management changes. Our diagnosis includes GenBank accession numbers for COI sequences to facilitate future molecular phylogenetic comparisons.

Abundance and Diversity of Snails in Various Agroecosystems in District Multan, Pakistan

Snails are the second largest group of invertebrates after insects and make a major part of the world's fauna. There are 35,000 described species of land snails (subclass: Pulmonata) from class gastropods in the world. With the present scenario of climate change, their occurrence has been observed many-fold. Despite their economic importance, population diversity and distribution of these creatures on different host crops in Punjab Pakistan is not much more studies. For this purpose, the present study was conducted to identify the snail species and their diversity on different hosts in the agroecosystems of Multan. The snails were collected from different crops like wheat, vegetable (spinach), fodder, citrus orchard, and plant nurseries. A total of 8264 specimens were collected and preserved in the form of a dry shell and 70% alcohol in Ecology Lab, MNS - the University of Agriculture, Multan for identification purpose. The preserved specimens were identified under microscope using available taxonomic keys. Ariophanta bistrialis ceylanica, Ariophanta bistrialis cylix, Ariophanta solata, Oxychilus draparnaudi, Monacha catiana, Cernuella virgata, Pupoides abilabris and Oxyloma elegans were identified during the study. The highest and lowest population of Ariophanta bistrialis cylix (1592) and Ariophanta solata (446), respectively was recorded. The population diversity was found maximum in plant nurseries followed by fodder crop (Berseem) and citrus orchard while least in vegetable (Spinach) and wheat crop. The maximum population was found in July and August, while least in March and May. In the rainy season, the population of snails was recorded maximum as compared to the dry season.

Molecular data from up to 130‐year‐old herbarium specimens do not support the presence of cherry powdery mildew in Australia

AbstractA strain of Podosphaera clandestina has been highlighted as a priority pest threat to the Australian cherry industry. Australia currently has no records of powdery mildew on cherry (Prunus avium). P. clandestina is reported to cause disease on a range of Rosaceae genera including Crataegus and Prunus; in Australia, P. clandestina has only been recorded on Crataegus. A recent species revision identified Podosphaera cerasi on P. avium as a separate species from P. clandestina. Therefore, a revision of which powdery mildew species is present in Australia on Crataegus is required to inform Australian plant biosecurity. Reference collection specimens from the Victorian Plant Pathology Herbarium (VPRI) recorded as Podosphaera spp. collected between 1889 to 2008 on cherry and three other host plant genera from Australia and overseas were sampled for DNA extraction and next‐generation sequencing (NGS). Sequence data from preserved specimens were successfully mapped to internal transcribed spacer (ITS) sequences of P. clandestina in the strict sense, P. cerasi, and Podosphaera prunicola, and chloroplast matK sequences were used to identify plant hosts. Australian specimens on Crataegus hosts were P. clandestina in the strict sense and specimens on Prunus from the USA were identified as P. cerasi and P. prunicola. The outcome of this study confirmed the powdery mildew on Australian Crataegus specimens to be P. clandestina and none of the cherry powdery mildews (Podosphaera pruni‐avium, P. cerasi, or P. prunicola) are present on Australian specimens in the VPRI collection, which suggests they are not present in Australia.

The Palestinian Terrestrial Vertebrate Fauna Preserved at the Biology Exhibitions of the Universities of the Gaza Strip

The Gaza Strip (365 km2 ) of Palestine (27,000 km2 ) is home to a wealth of terrestrial vertebrate fauna. Some of these faunistic species find their ways to preservation at the local universities. Hence, the current study comes to document the Palestinian terrestrial vertebrate fauna acquired by the biology exhibitions (BEs) of Al-Azhar University, Islamic University of Gaza and Al-Aqsa University that are located at the Gaza City of the Gaza Strip. The amphibians, reptiles, birds and mammals preserved at BEs of the universities in question were surveyed and scientifically classified during a three-month period extending from January to March, 2012. The study showed that all BEs of local universities are underdeveloped, lacking attention and suffer from specimen scarcity and good preservation. The BE at Al-Azhar University is the best in the arrangement and preservation of bird specimens. A total number of 200 specimens belonging to 54 terrestrial vertebrate fauna species, 39 families and 17 orders was recorded at BEs. Reptiles constituted 40.7% of the total species recorded, followed by birds (38.9%), mammals (14.8%) and amphibians (5.6%). The Islamic University of Gaza was considered the best in terms of the number of preserved species (39.8%), followed by Al-Azhar University (36.3%) and Al-Aqsa University (23.9%). The Common Toad (Bufo viridis) was the most preserved among the amphibian species recorded. Squamata was the biggest reptilian order, comprising 20 species (8 lizards and 12 snakes), with the Syrian Black Snake (Coluber jugularis asianus) was the commonest. The Palestine Viper (Vipera palaestinae) is endemic to Palestine and most venomous and dangerous to human health. The Great White Pelican (Pelecanus onocrotalus) was the largest Palestinian bird preserved at BE of Al-Azhar University. The Egyptian Mongoose (Herpestes ichneumon) and the Common Badger (Meles meles) were the biggest mammalian specimens preserved, while the Palestine Mole-rat (Spalax leucodon ehrenbergi) was the only Palestine endemic species encountered among the preserved mammals. Finally, the improvement of BEs of local universities and the construction of a Central Museum of Natural History is highly recommended in order to change the Palestinians’ attitudes toward a sustainable ecological conservation in the Gaza Strip.

A new tale of lost tails: Correlates of tail breakage in the worm lizard Amphisbaena vermicularis.

Predator–prey interactions are important evolutionary drivers of defensive behaviors, but they are usually difficult to record. This lack of data on natural history and ecological interactions of species can be overcome through museum specimens, at least for some reptiles. When facing aggressive interactions, reptile species may exhibit the defensive behavior of autotomy by losing the tail, which is also known as “urotomy”. The inspection of preserved specimens for scars of tail breakage can reveal possible ecological and biological correlates of urotomy. Herein, we investigated how the probability of urotomy in the worm lizard Amphisbaena vermicularis is affected by sex, body size, temperature, and precipitation. We found higher chances of urotomy for specimens with larger body size and from localities with warmer temperatures or lower precipitation. There was no difference in urotomy frequency between sexes. Older specimens likely faced – and survived – more predation attempts through their lifetime than smaller ones. Specimens from warmer regions might be more active both below‐ and aboveground, increasing the odds to encounter predators and hence urotomy. Probability of urotomy decreased with increased precipitation. Possibly, in places with heavier rainfall worm lizards come more frequently to the surface when galleries are filled with rainwater, remaining more exposed to efficient predators, which could result in less survival rates and fewer tailless specimens. This interesting defensive behavior is widespread in squamates, but yet little understood among amphisbaenians. The novel data presented here improve our understanding on the correlates of tail breakage and help us to interpret more tales of lost tails.

First occurrence of <em>Lindenia tetraphylla</em> (Vander Linden, 1825) for Northern Lazio (Odonata, Gomphidae)

[The presence of the Bladetail, Lindenia tetraphylla, is reported in the Mignone River Valley, Tarquinia (VT) (ZSC IT6010035 “River Mignone - lower course”) reconfirming this gomphid for Lazio. This rare dragonfly is localized in Italy and is reported in a few locations in Tuscany, Campania, Umbria, Molise, Abruzzo, Puglia, Sicilia and Sardinia. Lindenia tetraphylla was previously reported for a locality in southern Lazio (Lago di Fondi, LT) on the basis of two preserved specimen in the Entomological Collection A. Costa of the Museum of Zoology of the Naples University in and dating back to the midnineteenth century. The habitat of the species is made up of the gravelly shore and the curtain of willows, poplars and tamarisks of one of the best-preserved rivers in Lazio]. 
 [Article in Italian]

Scope and Features of Museum Setup Techniques in Oral Pathology

The preservation of pathological specimens has become very significant as it is a potential teaching tool and important source of data. Museum has been made compulsory in medical and dental schools by various countries as it is not a mere storage of old and rare specimens, but a vital learning aid in the field of medicine. The procedure of museum setup requires both scientific and technical skills. The term ‘Museum technology’ together constitutes the method of collection, preservation and storage of specimens. This article aims to review the basics in setting up a museum. Emerging advancements in museum techniques are also highlighted.

Description of two new miniature species of Hylopanchax Poll & Lambert, 1965 (Cyprinodontiformes: Procatopodidae), from northeastern Gabon, with an updated diagnosis of the genus based on morphology, colouration and osteology.

Two new species of the lampeye genus Hylopanchax are described from the Ivindo River basin in the Ogowe River drainage. Hylopanchax multisquamatus, new species, and Hylopanchax thysi, new species, differ from congeners by the presence of a hyaline urogenital male papilla with small black spots and a dark-brown reticulate pattern on the flanks of both males and females in preserved specimens. Hylopanchax multisquamatus is distinguished from congeners by the number of scales on the mid-longitudinal series (27-30 vs. 19-26, respectively) and by the relative anterior/posterior flank scale depth ratio (140%-150% vs. 170%-220%). Hylopanchax thysi is distinguished from all other congeners, except Hylopanchax paucisquamatus, by the presence of vertebrae (30 vs. 31-33) and is further distinguished from H. multisquamatus by the presence of a deeper caudal peduncle and much larger anterior flank scales. It is distinguished from H. paucisquamatus by the presence of a hyaline urogenital male papilla with small black spots and a dark-brown reticulate pattern on the flanks of both males and females in preserved specimens. Osteological data of Hylopanchax are presented for the first time, and an updated diagnosis based on external morphology, colouration pattern and osteology is provided. An osteological comparison with closely related species belonging to the genera Procatopus, Hypsopanchax and "Hypsopanchax" is presented. (a) A truncate and slightly downward-directed anterior process of the angulo-articular and (b) a guitar-shaped lachrymal with both its anterior and posterior margins sharply curved are here considered as diagnostic features of Hylopanchax.

<p><strong>Two new Mesozoic species of Tipulomorpha (Diptera) from the Teete locality, Russia </strong></p>

In Siberia, there are numerous Jurassic and Cretaceous locations abundant in fossil insects. The Teete outcrop (renamed Kempendyay) located in the eastern Siberia (Russia) is an important site due to its age estimated to beginning of the Lower Cretaceous as well as its high taxa diversity and very good specimen preservation. However, it is poor in fossils of the Limoniidae. Study of the rare Tipulomorpha material from the Teete locality (= Kempendyay) is presented herein and two new species are described and illustrated. One of them belongs to the family Pediciidae, Prearchitipula ryszardi sp. nov., and the second to the family Limoniidae, Eotipula wieslawi sp. nov. Both species are dedicated to two well-known Polish specialists of fossil Diptera Nematocera.

Redescription and molecular characterization of Crenidens macracanthus Günther, 1874 (Pisces: Sparidae), and first record of C. indicus Day, 1873 in the eastern Indian Ocean

AbstractCrenidens macracanthus was originally described in 1874 based on a single specimen collected from Chennai (Madras), south-east coast of India. In 1875, the species was synonymized with C. indicus without citing any valid reason. Since then, no taxonomic studies have been attempted for the genus Crenidens, except in 2013 the species was resurrected from synonymy and redescribed as a valid species based on the holotype and non-type specimen. In view of the fact that C. macracanthus is a poorly known species, it is redescribed based on examination of 30 additional specimens of 105.8–162.2 mm SL, collected from Puri, Odisha, north-east coast of India (Bay of Bengal) from 2017–2019, using morphological and molecular examinations. Our study provides a detailed morphological description, first colour photographs and phylogenetic analysis using COI barcodes of the species. The study has expanded the range in several morpho-meristic characters in comparison with the type and non-type specimens described earlier. The species in fresh condition can be easily distinguished from its two congeners (C. crenidens and C. indicus) by the yellowish tip of the lower caudal-fin lobe. Our study has also extended the distribution range of C. indicus (previously known only from the north-eastern Arabian Sea) to the eastern Indian Ocean, based on examination of a preserved specimen collected from Tuticorin, Tamil Nadu.

The reproduction of human pathology specimens using three-dimensional (3D) printing technology for teaching purposes

The teaching of medical pathology has undergone significant change in the last 30–40 years, especially in the context of employing bottled specimens or ‘pots’ in classroom settings. The reduction in post-mortem based teaching in medical training programs has resulted in less focus being placed on the ability of students to describe the gross anatomical pathology of specimens. Financial considerations involved in employing staff to maintain bottled specimens, space constraints and concerns with health and safety of staff and student laboratories have meant that many institutions have decommissioned their pathology collections. This report details how full-colour surface scanning coupled with CT scanning and 3 D printing allows the digital archiving of gross pathological specimens and the production of reproductions or replicas of preserved human anatomical pathology specimens that obviates many of the above issues. With modern UV curable resin printing technology, it is possible to achieve photographic quality accurate replicas comparable to the original specimens in many aspects except haptic quality. Accurate 3 D reproductions of human pathology specimens offer many advantages over traditional bottled specimens including the capacity to generate multiple copies and their use in any educational setting giving access to a broader range of potential learners and users.

First record of Thamnodynastes almae Franco & Ferreira, 2002 (Serpentes, Dipsadidae, Xenodontinae) in the state of Piauí, northeastern Brazil, and updated distribution map

We report for the first time the snake Thamnodynastes almae Franco & Ferreira, 2002 in Piauí, northeastern Brazil. Our record is based on two specimens and comprises the 17th known locality for the species. The new record represents the northernmost and westernmost locality, at the limit between the Caatinga and the Maranhão Babaçu Forest ecoregions, and extends this species’ geographic distribution 495 km from Milagres, state of Ceará. Updated distribution maps and images of preserved specimens are provided.

Revision of Botrychiopsis plantiana: A Key Species of the Gzhelian–Cisuralian in Westernmost Gondwana

Botrychiopsis is an important biostratigraphic index taxon for Carboniferous–early Permian plant fossil successions of Gondwana. Its species have wide geographic distributions, restricted chronostratigraphic ranges, and are associated with diverse groups of plant associations. They have been described from sedimentary sequences of Australia, India, South Africa, Brazil, and Argentina. A whole-frond reconstruction of Botrychiopsis plantiana is presented based on a study of very well preserved specimens from the Gangamopteris flora (Ghzelian–Asselian) of the Bajo de Véliz Formation (Paganzo Basin, Argentina). The material consists of impressions of complete fronds and basal fragments, which are rarely represented in the Gondwanan upper Paleozoic fossil record.

Recent confirmed records of Galictis vittata in the department of Sucre, Caribbean region of Colombia

Galictis vittata is a small carnivore distributed in all the natural regions of Colombia, but it is considered as a “demographic rarity” due to its low density throughout its range in the national territory. This note documents new records with some ecological data and compiles the existing evidence about the local distribution of G. vittata in the department of Sucre, Colombian Caribbean. The new records are the product of fieldwork aiming at evaluating mammal diversity in the region, among other ecological aspects, by applying camera-trapping and interviews. In addition, other confirmed records of the species in the department were compiled based on the available information in three online data portals. The new records of G. vittata presented include a camera-trap record in a disturbed area of tropical dry forest and one related to an event of attack by domestic dogs. The local distribution of the species is extended to the sub-regions of Golfo de Morrosquillo and San Jorge, based on that three preserved specimens confirm its presence in the Montes de Maria sub-region. This is among the first data compilation of G. vittata focused specifically to the department of Sucre, also confirming its current presence in disturbed zones of tropical dry forest. The new records contribute evidence to previous statements about the potential ecological tolerance of the species to landscape modifications and provide novel information on possible threats and habitat use, specially about the potential importance of some elements in fragmented landscapes.

Aegean monkeys and the importance of cross-disciplinary collaboration in archaeoprimatology: a reply to Urbani and Youlatos.

In their reply to our article "A new identification of the monkeys depicted in a Bronze Age wall painting from Akrotiri, Thera" [Primates 61(3), 2019], Urbani and Youlatos (Primates https://doi.org/10.1007/s10329-020-00825-2 , 2020) argue for the traditional identification of the monkeys depicted on the north and west walls of Room 6 of Building Complex Beta at Akrotiri, Thera, as vervet monkeys (Fig.1). Their argument is based largely on previous scholarship and their analysis of monkey morphology as it appears in this Bronze Age artwork. Here, after clarifying some misconceptions and misquotations, we thoroughly contextualize the wall painting in question, emphasizing the importance of collaboration between disparate disciplines for a multifaceted and rigorous approach. The nature of the item in question is key in this reply: it is an artwork. Because the artwork in question is a cultural representation of monkeys rather than a study of live primates or preserved specimens, consideration of artistic choice, color conventions, and the agency of the artist, which are important facets of material culture, is important when answering the questions raised by Urbani and Youlatos, and should stimulate further cross-disciplinary discussions.

Osteology of the Italian endemic spectacled salamanders, Salamandrina spp. (Amphibia, Urodela, Salamandridae): selected skeletal elements for palaeontological investigations.

Salamandrina perspicillata, from Northern and Central Italy, and Salamandrina terdigitata, from Southern Italy, represent a unique case of endemism among the amphibians of Europe. Some efforts were made to study the biology and ecology of these species, but only few studies focused on their comparative osteology. In particular, detailed descriptions of isolated skeletal elements and comparisons with other European urodeles are not available in literature hindering the identification of their fossils that testify for an ancient much broader range. The correct identification of fossil remains, mostly based on careful osteological descriptions, is fundamental to study the evolution of the range of this genus through time and the origin of the current endemic condition. This work is focused on the description of selected skeletal elements (occipito-otic complex, limb bones, atlas, trunk, sacral, caudosacral and caudal vertebrae) of dry-prepared skeletons and CT-scans of wet preserved specimens, which are the most common in the fossil record. We provide osteological comparison of Salamandrina with other genera of Europe, yielding diagnostic characters which allow the identification of fossils at a generic level. No significant differences between the two species S. perspicillata and S. terdigitata were found in the described skeletal elements, thus, the identification of skeletal elements at the species level is, at the moment, impossible.

Fool's gold, fool's paradise? Utilising X-ray micro-Computed Tomography to evaluate the effect of environmental conditions on the deterioration of pyritic fossils

Natural history museums hold hundreds of thousands of mineral and fossil specimens containing iron sulfides, such as pyrite, all of which may be at risk from deterioration. Oxidation of these minerals causes cracking, crystal growth and powdering which, if unchecked, can eventually lead to the complete loss of specimens. This article reports the findings of an experiment which utilised X-ray micro-Computed Tomography (μCT) to generate 3D time lapse imaging over 15 months to compare the deterioration of pyritic ammonites stored within micro-environments created with varying levels of oxygen and relative humidity. The study concludes that the optimum storage parameters for the preservation of sulfide-bearing specimens are 40% relative humidity and 0% oxygen.

Wildlife collection for scientific purposes.

Illegal transfer of wildlife has 2 main purposes: trade and scientific research. Trade is the most common, whereas scientific research is much less common and unprofitable, yet still important. Biopiracy in science is often neglected despite that many researchers encounter it during their careers. The use of illegally acquired specimens is detected in different research fields, from scientists bioprospecting for new pharmacological substances, to taxonomists working on natural history collections, to researchers working in zoos, aquariums, and botanical gardens. The practice can be due to a lack of knowledge about the permit requirements in different countries or, probably most often, to the generally high level of bureaucracy associated with rule compliance. Significant regulatory filters to avoid biopiracy can be provided by different stakeholders. Natural history collection hosts should adopt strict codes of conduct; editors of scientific publications should require authors to declare that all studied specimens were acquired legally and to cite museum catalog numbers as guarantee of best practices. Scientific societies should actively encourage publication in peer-reviewed journals of work in which specimens collected from the wild were used. The International Commission on Zoological Nomenclature could require newly designated types based on recently collected specimens to be accompanied by statements of deposition in recognized scientific or educational institutions. We also propose the creation of an online platform that gathers information about environmental regulations and permits required for scientific activities in different countries and respective responsible governmental agencies and the simplification of the bureaucracy related to regulating scientific activities. This would make regulations more agile and easier to comply with. The global biodiversity crisis means data need to be collected ever faster, but biopiracy is not the answer and undermines the credibility of science and researchers. It is critical to find a modus vivendi that promotes compliance with regulations and scientific progress.