Specific Recognition Sites Research Articles

Advances in application of molecularly imprinted polymers to the detection of polar pesticide residues

极性农药包括杀菌剂、除草剂、杀虫剂等,种类丰富,成本低廉,在农业中应用广泛,其滥用易导致水资源和土壤等环境污染,人类通过间接接触动植物源性食品和环境中的极性农药残留也增加了农药暴露风险。极性农药的物理化学性质差异大,通常痕量存在于食品和环境样品等复杂基质中,这对其准确检测分析带来了挑战。分子印迹聚合物(MIPs)作为一种人工制备的选择性吸附剂,具有与模板分子在空间结构、大小尺寸和功能基团上互补的特定识别位点,且易于制备,成本低,稳定性好,重复利用率高,已被广泛用于极性农药残留的样品前处理和分析检测中。MIPs可以作为固相萃取(SPE)、固相微萃取(SPME)、磁性固相萃取(MSPE)、搅拌棒固相萃取(SBSE)等前处理方法的吸附剂,还可用于制备光、电、化学传感器,作为质谱检测的离子源基底和拉曼光谱的增强基底。目前针对极性农药残留的检测,已有许多研究报道了多种分子印迹材料用于高效分离分析各种复杂基质中的极性农药残留,但未见此方面的综述报道。该文首先介绍了MIPs的印迹策略、聚合策略,并针对传统MIPs制备和应用中存在的问题,简要概括了一些新型的分子印迹策略和制备技术;然后从极性农药残留分析的角度出发,总结归纳了分子印迹材料近年来特别是近5年来在各种极性农药残留(包括新烟碱类、有机磷类、三嗪类、唑类、脲类等)检测中的应用,并针对现存问题展望了其未来的发展方向和趋势。

A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor

Staphylococcus aureus is one of the major foodborne pathogens. Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial. Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor. The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus. For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan. The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements. To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.

Combination of bidirectional strand displacement amplification with single-molecule detection for multiplexed DNA glycosylases assay

DNA glycosylases can initiate base excision repair pathway to repair endogenous DNA base damages for the maintenance of genome stability. Multiple DNA glycosylases exhibit abnormal in various diseases, and the simultaneous measurement of different DNA glycosylases is critical to clinical diagnosis and drug discovery. Herein, we take advantage of single-molecule detection and bidirectional strand displacement amplification (SDA) to simultaneously detect uracil DNA glycolase (UDG) and human alkyladenine DNA glycosylase (hAAG). We design a partial double-stranded DNA (dsDNA) substrate modified with specific recognition sites of UDG and hAAG. The dsDNA substrate is labeled with BHQ1 and BHQ2 at the 5′-ends and then hybridizes with the Cy3/Cy5-labeled reporter probes to obtain the BHQ1/Cy3 and BHQ2/Cy5 base pairs, resulting in the quenching of Cy3/Cy5 fluorescence by BHQ1/BHQ2 via fluorescence resonance energy transfer (FRET). When UDG and hAAG are present, they can induce the base excision repair reaction and subsequently initiate the bidirectional SDA amplification process, releasing the Cy5/Cy3-labeled reporter probes from the dsDNA substrate and consequently the recovery of Cy5 and Cy3 fluorescence, which can be measured by single-molecule detection, with Cy5 indicating UDG and Cy3 indicating hAAG. This method possesses high sensitivity and good selectivity with the capability of quantifying multiple DNA glycosylases at the single-cell level. Furthermore, it can be used to simultaneously screen DNA glycosylase inhibitors and determine enzyme kinetic parameters, with the potential of sensing various DNA/RNA enzymes by simple changing the recognition sites of DNA substrates.

A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications

The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker α-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring α-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 × 10−4 to 0.60 mg mL−1 in buffer solution and between 3.0 × 10−4 to 3.0 × 10−2 mg mL−1 in human saliva) and low detection levels were achieved (LOD < 3.0 × 10−4 mg mL−1) using square wave voltammetry (SWV) as the electroanalytical technique.

Identification of a Novel Cleavage Site and Confirmation of the Effectiveness of NgAgo Gene Editing on RNA Targets.

Clusters of regularly interspaced short palindromic repeats (CRISPR)/Cas systems have a powerful ability to edit DNA and RNA targets. However, the need for a specific recognition site, protospacer adjacent motif (PAM), of the CRISPR/Cas system limits its application in gene editing. Some Argonaute (Ago) proteins have endonuclease functions under the guidance of 5' phosphorylated or hydroxylated guide DNA (gDNA). The NgAgo protein might perform RNA gene editing at 37°C, suggesting its application in mammalian cells; however, its mechanisms are unclear. In the present study, the target of NgAgo in RNA was confirmed in vitro and in vivo. Then, an in vitro RNA cleavage system was designed and the cleavage site was verified by sequencing. Furthermore, NgAgo and gDNA were transfected into cells to cleave an intracellular target sequence. We demonstrated targeted degradation of GFP, HCV, and AKR1B10 RNAs in a gDNA-dependent manner by NgAgo both in vitro and in vivo, but no effect on DNA was observed. Sequencing demonstrated that the cleavage sites are located at the 3' of the target RNA which is recognized by 5' sequence of the gDNA. These results confirmed that NgAgo-gDNA cleaves RNA not DNA. We observed that the cleavage site is located at the 3' of the target RNA, which is a new finding that has not been reported in the past.

Cyclometalated Iridium(III) Complex-Sensitized NiO-Based-Cathodic Photoelectrochemical Platform for DNA Methyltransferase Assay.

This work reports an efficient [(C6)2Ir(dppz)]+PF6- (C6 = coumarin 6 and dppz = dipyridophenazine)-sensitized NiO photocathode and its application in photoelectrochemical (PEC) bioanalysis field for the first time. This dye-sensitized NiO photocathode was found to exhibit a markedly enhanced cathodic photocurrent. A sensitive cathodic PEC platform was proposed integrating the as-prepared photocathode with enzyme-free cascaded amplification strategies of the catalytic hairpin assembly (CHA) and the hybridization chain reaction (HCR) for DNA methyltransferase (MTase) assay. A hairpin DNA(HDam) with specific recognition site of Dam MTase in its stem was designed. The site of HDam was methylated in the presence of Dam MTase and then cut by endonuclease DpnI. The released loop fragment, as an initiator, triggered the CHA circuit and the follow-up HCR circuit, resulting in long dsDNA concatemers on the ITO electrode. Numerous [(C6)2Ir(dppz)]+PF6- were intercalated into dsDNA, and highly efficient signal amplification was realized. Benefiting from the superior iridium(III) complex-sensitized NiO photocathode and effective amplification strategy, a detection limit of 0.0028 U/mL for the determination of Dam MTase was achieved. Moreover, this work further demonstrated that these proposed tactics could be applied to screen Dam MTase activity inhibitors.

Nucleation of protein mesocrystals via oriented attachment

Self-assembly of proteins holds great promise for the bottom-up design and production of synthetic biomaterials. In conventional approaches, designer proteins are pre-programmed with specific recognition sites that drive the association process towards a desired organized state. Although proven effective, this approach poses restrictions on the complexity and material properties of the end-state. An alternative, hierarchical approach that has found wide adoption for inorganic systems, relies on the production of crystalline nanoparticles that become the building blocks of a next-level assembly process driven by oriented attachment (OA). As it stands, OA has not yet been observed for protein systems. Here we employ cryo-transmission electron microscopy (cryoEM) in the high nucleation rate limit of protein crystals and map the self-assembly route at molecular resolution. We observe the initial formation of facetted nanocrystals that merge lattices by means of OA alignment well before contact is made, satisfying non-trivial symmetry rules in the process. As these nanocrystalline assemblies grow larger we witness imperfect docking events leading to oriented aggregation into mesocrystalline assemblies. These observations highlight the underappreciated role of the interaction between crystalline nuclei, and the impact of OA on the crystallization process of proteins.

(Macro)Molecular Imprinting of Proteins on PCL Electrospun Scaffolds.

Biological recognition sites are very useful for biomedical purposes and, more specifically, for polymeric scaffolds. However, synthetic polymers are not capable of providing specific biological recognition sites. To solve this inconvenience, functionalization of biological moieties is typically performed, oftentimes via peptide binding. In this sense, the main task is capturing the biological complexity of a protein. This study proposes a possible alternative solution to this challenge. Our approach is based on the combination of molecular imprinting (MI) and electrospinning processes. We propose here an alternative MI approach with polymeric structures, instead of using cross-linkers and monomers as conventionally performed. Different PCL–protein scaffolds were produced via electrospinning before performing MI. Gelatin, collagen, and elastin were used as proteins. Results evidenced that the MI process conducted with PCL electrospun membranes was carried out with ionic interactions between the desired molecules and the recognition sites formed. In addition, it has been proved that MI was more efficient when using gelatin as a template. This approach opens a new stage in the development of recognition sites in scaffolds obtained with synthetic polymers and their application for biomedical purposes.

Novel Molecular Organic Framework Composite Molecularly Imprinted Nanofibrous Membranes with a Bioinspired Viscid Bead Structure for Selective Recognition and Separation of Atrazine.

In this work, novel atrazine (ATZ) molecularly imprinted nanofibrous membranes (A-MNMs) with a molecular organic framework (MOF)-based viscid bead structure were developed based on a natural spider-web-inspired strategy for selective separation of ATZ. Poly(vinylidene fluoride)/poly(vinyl alcohol) (PVDF/PVA) blended nanofibrous membranes as the basal membrane were synthesized by electrospinning technology combined with a chemical cross-linking procedure. The most critical design is that MOF nanocrystals as the matrix of the viscid bead structure were assembled on the PVDF/PVA blended nanofibrous membrane surface and the specific recognition sites were efficiently constructed on the surface and pores of the MOF-based viscid bead structure by a surface imprinting strategy. Significantly, the as-synthesized MOF-based viscid bead structure has an enhanced specific surface area, which helps to form abundant specific recognition sites in A-MNMs. Therefore, the A-MNMs with a spider-web-like structure presented an enhanced rebinding capacity (37.62 mg g-1) and permselectivity (permselectivity factors β were 4.21 and 4.31) toward ATZ. Moreover, the A-MNMs display strong practicability in separation of ATZ from simulated environmental water samples. The presented work has shown tremendous potential for preparing natural spider-web-like molecularly imprinted membranes (MIMs) for selective separation of environment pollutants.

Reduced Graphene Oxide Fabricated Molecular Imprinted Polymeric Layers for Improved Recognition of Ciprofloxacin

Introduction Ciprofloxacin is a broad spectrum synthetic antibiotic that is frequently used against gram negative and gram positive bacteria. The increasing use of ciprofloxacin by humans has also results its release in environment as it is detected in different water bodies [1]. The presence of antibiotics in aquatic environment could result the development of antibiotic resistant strains [2], which is a serious concern. The current analytical tools for its sensitive detection are mainly based on spectrometric or chromatographic techniques. Nevertheless these methods are time consuming and require extensive sample preparation steps. It is imperative to design sensitive and robust sensor setups for its rapid detection in water bodies. In this study, we designed molecular imprinted polymeric layers incorporated with graphene derivatives for electrochemical sensing of ciprofloxacin using interdigitated capacitors as transducer. These devices offer rapid response and have low fabrication costs. The combination of reduced graphene oxide (rGO) with molecular imprinted polymer (MIP) has shown enhanced sensitivity and selectivity thus, developing smart sensor devices for straightforward detection of ciprofloxacin. Experimental Graphene oxide (GO) was synthesized by oxidation of graphite powder following modified Hummer’s method. For preparing reduced graphene oxide (rGO), the as-prepared GO was dispersed in distilled water and treated with sodium borohydride as reducing agent at 70°C for about 12 hours. The resultant product was subjected to several washings and dried overnight at 65°C. Molecular imprinted polymer (MIP) was prepared using methacrylic acid (MAA) and ethyleneglycol dimethacrylate (EGDMA) along with ciprofloxacin as template structure in dimethylformamide (DMF). The as-prepared GO/rGO powder was added to above pre-polymer mixture to prepare respective composite coatings. Azoisobutyrnitrile (AIBN) was used to carry out thermally initiated free radical polymerization at 60 °C for 30 minutes. The resultant polymer mixtures were integrated with IDCs by spin coating. The non-imprinted polymer was prepared under same manner without adding template. Moreover, its composite layers with GO/rGO were prepared under similar conditions and integrated with IDCs. Results and Conclusion The sensor response as capacitance shift for rGO-MIP, GO-MIP, MIP and their corresponding non-imprinted layers i.e., rGO-NIP, GO-NIP and NIP was measured for varying concentrations of ciprofloxacin as shown in figure 1. Comparing the response of imprinted layers, it can be noticed that rGO-MIP coated IDCs showed highest sensor shift i.e. 43 µF, while GO-MIP is second best (34 µF) and finally, the pristine MIP is at third place (25 µF). This suggests that incorporation of rGO in MIP [3] enhances the sensor shifts and thus, is a suitable strategy for improving the sensitivity. On other hand, all the non-imprinted layers i.e., rGO-NIP, GO-NIP and pristine NIP showed much lower sensor shifts. Moreover, these layers showed similar sensor shifts indicating that these layers does not possess any specific recognition sites and thus, can be taken as control surface.The selectivity of rGO-MIP sensor is tested by exposing the device against structurally analogous antibiotic drugs. The sensor shifts of rGO-MIP for ciprofloxacin, levofloxacin and moxifloxacin in the concentration range 10-50 ppm is shown in figure 2. It is evident that sensor response for ciprofloxacin is at least 3 times higher than levofloxacin and moxifloxacin. The significantly higher sensor shifts for ciprofloxacin is due to the presence of complementary interaction sites in MIP layer. While levofloxacin and moxifloxacin despite of their similar structures to ciprofloxacin showed much lower sensor shifts. The results suggested that the incorporation of rGO to MIP not only improves sensitivity but also contribute in enhanced selectivity as well.

3D printed hybrid bone constructs of PCL and dental pulp stem cells loaded GelMA.

Fabrication of scaffolds using polymers and then cell seeding is a routine protocol of tissue engineering applications. Synthetic polymers have adequate mechanical properties to substitute for some bone tissue, but they are generally hydrophobic and have no specific cell recognition sites, which leads to poor cell affinity and adhesion. Some natural polymers, have high cell affinity but are mechanically weak and do not have the strength required as a bone supporting material. In the present study, 3D printed hybrid scaffolds were fabricated using PCL and GelMA carrying dental pulp stem cells (DPSCs), which is printed in the gaps between the PCL struts. This cell loaded GelMA was shown to support osteoinductivity, while the PCL provided mechanical strength needed to mimic the bone tissue. 3D printed PCL/GelMA and GelMA scaffolds were highly stable during 21 days of incubation in PBS. The compressive moduli of the hybrid scaffolds were in the range of the compressive moduli of trabecular bone. DPSCs were homogeneously distributed throughout the entire hydrogel component and exhibited high cell viability in both scaffolds during 21 days of incubation. Upon osteogenic differentiation DPSCs expressed two key matrix proteins, osteopontin and osteocalcin. Alizarin red staining showed mineralized nodules, which demonstrates osteogenic differentiation of DPSCs within GelMA. This construct yielded a very high cell viability, osteogenic differentiation and mineralization comparable to cell culture without compromising mechanical strength suitable for bone tissue engineering applications. Thus, 3D printed, cell loaded PCL/GelMA hybrid scaffolds have a great potential for use in bone tissue engineering applications.

The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity.

Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII. TkoI is encoded by TK1460 while TkoII is encoded by TK1158. Bioinformative analysis suggests both R-M enzymes are large, fused methyltransferase (MTase)-endonuclease polypeptides that contain both restriction endonuclease (REase) activity to degrade foreign invading DNA and MTase activity to methylate host genomic DNA at specific recognition sites. In this work, we demonsrate T. kodakarensis strains deleted for either or both R-M enzymes grow more slowly but display significantly increased competency compared to strains with intact R-M systems, suggesting that both TkoI and TkoII assist in maintenance of genomic integrity in vivo and likely protect against viral- or plasmid-based DNA transfers. Pacific Biosciences single molecule real-time (SMRT) sequencing of T. kodakarensis strains containing both, one or neither R-M systems permitted assignment of the recognition sites for TkoI and TkoII and demonstrated that both R-M enzymes are TypeIIL; TkoI and TkoII methylate the N6 position of adenine on one strand of the recognition sequences GTGAAG and TTCAAG, respectively. Further in vitro biochemical characterization of the REase activities reveal TkoI and TkoII cleave the DNA backbone GTGAAG(N)20/(N)18 and TTCAAG(N)10/(N)8, respectively, away from the recognition sequences, while in vitro characterization of the MTase activities reveal transfer of tritiated S-adenosyl methionine by TkoI and TkoII to their respective recognition sites. Together these results demonstrate TkoI and TkoII restriction systems are important for protecting T. kodakarensis genome integrity from invading foreign DNA.

Boronate affinity-modified magnetic β-cyclodextrin polymer for selective separation and adsorption of shikimic acid

Polymer of cyclodextrin (CD) is still of great sustainable interest in the scientific research attributing to its excellent physicochemical properties. Owing to its water solubility and superior binding capacity, CD is usually modified through various strategies to exploit its application area. Herein, for the first time, magnetic β-cyclodextrin (β-CD) polymer is endowed with boronate affinity property via mild chemical modifications to specifically separate natural drug raw material shikimic acid (SA). The uniform morphology, magnetic response ability and composition analysis of the porous adsorbent were highly supported by the characterizations. The capture process towards SA depending on the pH value is explored in detail by adsorption kinetics, equilibrium, selectivity and reusability. It is found that specific recognition sites decorated on the surface of magnetic β-CD provides strength support for selective adsorption and separation of shikimic acid. The prepared adsorbent shows high adsorption quantity (Q, mg g−1) of 31.60 mg g−1 at 25 °C, high specific recognition and good adsorption capacity (above 83.7%) and stability after five cycles. This study gives a new sight on the utilization of porous β-CD-based material by grafting functional boric acid group for specific capture of natural cis-diol-containing compounds.

Red-emitting fluorescent turn-on probe with specific isothiocyanate recognition site for cysteine imaging in living systems

Cysteine (Cys) is an effective biomarker in life systems and is closely related to a variety of diseases, so developing a specific and efficient detection method for Cys is of great significance. To date, extensive work has been undertaken toward this goal. However, the differentiation of Cys from other biothiols still represents a challenge from an experimental point of view. Toward this end, a selective and sensitive red-emitting probe (TMN-NCS) with an isothiocyanate (ITC)-based structure was proposed in this paper. A large Stokes shift (210 nm) was observed upon addition of Cys to a solution of TMN-NCS. In addition, TMN-NCS showed low toxicity, a low detection limit (120 nM), and excellent cell permeability. The results suggested that TMN-NCS holds great promise for biological applications.

Fluorescent sensing system based on molecularly imprinted phase-change microcapsules and carbon quantum dots for high-efficient detection of tetracycline

Aiming at enhancing the detection efficiency and identification accuracy of tetracycline under a high-temperature condition, this study focuses on an innovative fluorescent sensing system (MIP@CQD-PCM) based on molecularly imprinted phase-change microcapsules along with the carbon quantum dots (CQDs) embedded in their shell. This system was fabricated by microencapsulating n-eicosane as a phase change material (PCM) core within a CQDs-embedded SiO2 shell, followed by coating a tetracycline-templated molecularly imprinted polymer (MIP) layer onto the surface of the SiO2 shell. The specific recognition sites to tetracycline molecules were finally achieved by removal of tetracycline template from the MIP layer. Comprehensive characterizations and investigations on the structure and performance of the fluorescent sensing system were given to confirm its successful fabrication in accordance to our design strategy. The resultant MIP@CQD-PCM exhibits a satisfactory thermal storage capacity and phase-change cycle stability for temperature regulation and thermal management applications under a phase-change enthalpy of over 162 J/g. Most of all, a typical fluorescence-quenching effect was obtained from the combination of the CQDs embedded in the SiO2 shell and the tetracycline molecules adsorbed in the MIP layer. This makes the MIP@CQD-PCM achieve an enhanced capability for the fluorescence identification of tetracycline in a high-temperature environment through the in situ thermal management of its PCM core. The MIP@CQD-PCM also displays high selectivity and good reusability for tetracycline detection in industrial applications. This work provides a promising strategy for the design and development of fluorescent sensing systems with high recognition efficiency and identification accuracy in the detection of hazardous substances.

A novel composite of conductive metal organic framework and molecularly imprinted poly (ionic liquid) for highly sensitive electrochemical detection of bisphenol A

A novel composite of conductive metal organic framework and molecularly imprinted poly (ionic liquid) (CMOF-MIPIL) was prepared for highly sensitive detection of bisphenol A (BPA). Ni-based CMOFs with various organic ligands, i.e., 2,3,6,7,10,11-hexaiminotriphenylene, hexa-aminobenzene, and 2,3,6,7,10,11-hexahydroxytriphenylene were synthesized. The electrochemical properties of the obtained Ni-based CMOFs were investigated. The results demonstrated that Ni3(HITP)2 had the highest conductivity and largest effective surface area. CMOF-MIPIL was then prepared with Ni3(HITP)2 as supporter, BPA as template, and ILs as monomer and cross-linker. Under optimal conditions, CMOF-MIPIL sensor exhibited a linear range of BPA detection from 0.005 to 5.0 μM. The detection limit was 4.0 nM. Compared with MIPIL, Ni3(HITP)2, and CMOF-NIPIL-based sensors, the performance of this sensor for BPA detection was superior due to the excellent conductivity and the presence of specific recognition sites. In addition, CMOF-MIPIL sensor had excellent selectivity for BPA. The proposed assay was used for detection of BPA in environmental water samples, plastic bottle and fresh liquid milk samples with success.

Recent Advances of Molecularly Imprinted Polymers Based on Cyclodextrin.

Molecular imprinting polymers (MIPs), generally considered as artificial mimics that are comparable to natural receptor, are polymers with tailor-made specific recognition sites complementary to the template molecules in shape and size. As a class of supramolecular compounds, cyclodextrins (CDs) are flourishing in the field of molecular imprinting with their unique structural properties. This review presents recent advances in application of MIPs based on CDs during the past five years. The discussion is grouped according to the different role of CDs in MIPs, that is, functional monomer, carrier modifier, etc. Main focus is the application of CD-based MIP on sample preparation, detection, and sensing. Additionally, drug delivery with CD-based MIP is also briefly discussed. Finally, challenges and future prospects of application of CDs in MIP are elaborated.

Molecularly imprinted fluoroprobes doped with Ag nanoparticles for highly selective detection of oxytetracycline in real samples

A molecularly imprinted polymer (MIP), which is synthesized by a nanomolding process around a template, has emerged as a promising analytical tool for environmental quality monitoring and food safety test. In this work, a fluoroprobe with Ag-doped MIP nanolayer (16 nm thickness) is successfully prepared for the highly selective detection of oxytetracycline (OTC) in real samples (i.e. Yangtze River water, swine urine). In the MIP nanolayer, two functional monomers (i.e. 4-(2-acrylamidoethylcarbamoyl)-3-fluorophenylboronic acid, methacrylic acid) synergistically constitute the specific recognition sites. Meanwhile, the doped Ag enhances the detection sensitivity (with a detection limit of 5.38 nM) and accelerates the detection rate (within 2.5 min) even in real samples. Therefore, the present study paves the way for the preparation of MIP-based fluoroprobes, showing great prospects in environmental quality and food safety tests.

Detection of Melamine by Using Fluorescent Nanocomposites with Specific Recognition Sites

AbstractIn this study, the novel nanocomposite was prepared by the coating of the semiconductor quantum dots (QDs) with the molecularly imprinted shell by mini‐emulsion polymerization to form the specific recognition sites on the surface for the detection of melamine. After the characterization of nanocomposites, the detection performance of the nanosensor was investigated by fluorescence measurements in aqueous solutions and milk samples. The aqueous solutions of melamine were used for the detection of melamine and the optimum conditions were determined as pH 5.0, melamine concentration (100 ppm), interaction time (6 min), and temperature (40 °C). The competitor molecules such as aniline, phenol, and cyanuric acid were used to determine the selectivity of the quantum dot‐based nanosensor system. The limit of detection (LOD) was obtained as 0.14±0.08 ppm. The reusability performance was investigated for 20 cycles by using the same nanocomposites and relative standard deviation (RSD) values of the nanosensor system were calculated as 11 % after 20 recycles. According to the results, the nanosensor system has shown that it is a favorable alternative to existing systems for the melamine detection.

Deciphering the Importance of Glycosphingolipids on Cellular and Molecular Mechanisms Associated with Epithelial-to-Mesenchymal Transition in Cancer.

Every living cell is covered with a dense and complex layer of glycans on the cell surface, which have important functions in the interaction between cells and their environment. Glycosphingolipids (GSLs) are glycans linked to lipid molecules that together with sphingolipids, sterols, and proteins form plasma membrane lipid rafts that contribute to membrane integrity and provide specific recognition sites. GSLs are subdivided into three major series (globo-, ganglio-, and neolacto-series) and are synthesized in a non-template driven process by enzymes localized in the ER and Golgi apparatus. Altered glycosylation of lipids are known to be involved in tumor development and metastasis. Metastasis is frequently linked with reversible epithelial-to-mesenchymal transition (EMT), a process involved in tumor progression, and the formation of new distant metastatic sites (mesenchymal-to-epithelial transition or MET). On a single cell basis, cancer cells lose their epithelial features to gain mesenchymal characteristics via mechanisms influenced by the composition of the GSLs on the cell surface. Here, we summarize the literature on GSLs in the context of reversible and cancer-associated EMT and discuss how the modification of GSLs at the cell surface may promote this process.