Background: The post-activation performance enhancement (PAPE) protocol is frequently utilized to enhance athletic performance through high-intensity stimuli. Despite its popularity, the effectiveness and mechanisms of PAPE remain unclear, particularly in the context of improving jump performance in sports. Methods: A systematic review was conducted following the PRISMA guidelines. A comprehensive search was conducted across databases including Scopus, Web of Science, PubMed, and EBSCO up to 31 March 2024. Results: Initially, 481 studies were identified, of which 8 were finally included, involving a varied number of participants, each focusing on the impact of PAPE on jump performance. These studies predominantly indicated that PAPE protocols can lead to short-term improvements in jump performance. Two publications demonstrated a positive effect on jump height, and one of them reported a questionable effect on jump height in the initial time period, while the remaining five publications showed a positive impact on jump performance. The average increase in height achieved based on the intervention from the publications was 4.03% (p < 0.05). Variability in effectiveness was noted depending on the type of exercises used in the activation phase, timing of performance post activation, and individual physiological characteristics. Conclusions: PAPE protocols can effectively enhance jump performance in athletic populations, although outcomes vary based on protocol specifics and individual differences. These findings support the need for individualized approaches in the application of PAPE protocols to optimize athletic performance.