Specific Nutritional Requirements Research Articles

Strategies to Enhance Cultivation of Anaerobic Bacteria from Gastrointestinal Tract of Chicken.

The gastrointestinal tract (GIT) of chicken is a complex ecosystem harboring trillions of microbes that play a pivotal role in the host's physiology, digestion, nutrient absorption, immune system maturation, and prevention of pathogen intrusion. For optimal animal health and productivity, it is imperative to characterize these microorganisms and comprehend their role. While the GIT of poultry holds a reservoir of microorganisms with potential probiotic applications, most of the diversity remains unexplored. To enhance our understanding of uncultured microbial diversity, concerted efforts are required to bring these microorganisms into culture. Isolation and cultivation of GIT-colonizing microorganisms yield reproducible material, including cells, DNA, and metabolites, offering new insights into metabolic processes in the environment. Without cultivation, the role of these organisms in their natural settings remains unclear and limited to a descriptive level. Our objective is to implement cultivation strategies aimed at improving the isolation of a diverse range of anaerobic microbes from the chicken's GIT, leveraging multidisciplinary knowledge from animal physiology, animal nutrition, metagenomics, feed biochemistry, and modern cultivation strategies. Additionally, we aim to implement the use of proper practices for sampling, transportation, and media preparation, which are known to influence isolation success. Appropriate methodologies should ensure a consistent oxygen-free environment, optimal atmospheric conditions, appropriate host incubation temperature, and provision for specific nutritional requirements in alignment with their distinctive needs. By following these methodologies, cultivation will not only yield reproducible results for isolation but will also facilitate isolation procedures, thus fostering a comprehensive understanding of the intricate microbial ecosystem within the chicken's GIT.

A comparative analysis of the nutrient and phytochemical richness among different varieties of quinoa in China

AbstractQuinoa is a nutrient‐dense pseudocereal that has garnered global attention for its potential to bolster food security and nutrition. Despite its celebrated status, the detailed nutritional profiles of various quinoa varieties remain poorly understood, which poses a significant barrier to the strategic cultivation and utilization of quinoa's genetic diversity to combat malnutrition. The impetus for this research lies in the urgent need to identify superior quinoa strains that can be tailored to meet specific nutritional requirements and adapt to diverse agro‐ecological zones. Our findings reveal substantial variation in nutrient content across different quinoa varieties, highlighting the variety ZLZX‐8 as a particularly nutrient‐rich strain with the highest levels of protein, fat, essential fatty acids, amino acids, and key minerals such as Mg, K, and Zn. Moreover, ZLZX‐8's exceptional antioxidant capacity suggests it may have additional health benefits beyond its macronutrient profile. In contrast, ZLZX‐7 stands out for its dietary fiber and phenolic content, which are critical for digestive health and disease prevention, respectively. Meanwhile, ZLZX‐5, with its high starch content, could be better suited for energy production in dietary applications. Notably, the study also uncovers a correlation between grain color and nutrient profile, with colored quinoa varieties exhibiting superior fiber, inositol, phenolic content, and antioxidant activity compared to their white counterparts. This work lays the groundwork for an informed selection of quinoa varieties that can enhance dietary quality, support local and global food systems, and contribute to the fight against malnutrition.

Nutritional status and Mediterranean diet adherence of children with Down Syndrome and their siblings

Objectives This study aimed to investigate the nutritional status, eating practices, dietary intake, and dietary patterns of Down Syndrome (DS) individuals and their non-DS siblings. Methods This cross-sectional study was carried out with two groups, consisting of 34 DS individuals and 23 siblings. Socio-demographic data, anthropometric measurements, food consumption, and feeding difficulties were recorded. Dietary patterns are evaluated by the Mediterranean Diet Quality Index (KIDMED). The physical activity was measured with the Physical Activity Questionnaire for Older Children (PAQ-C). Results The rates of being overweight and obese were higher in the DS group according to the WHO BMI-z scores classification (p = 0.002). Even using Turkish DS-specific growth curves, being overweight and obese rates were higher (p = 0.041). No statistical difference was found in energy, macronutrient intake, and the mean KIDMED scores. However, most of the DS group had moderate adherence and most siblings had high adherence to the Mediterranean diet (p = 0.037). The PAQ-C scores were lower in the DS group (p = 0.039). Conclusions We conclude that DS individuals have higher rates of being overweight/obese even using DS-specific growth curves despite similar dietary patterns and nutrient intake. To prevent obesity in DS, it is important to evaluate their growth considering specific nutritional requirements.

Sex differences in foraging ecology of a zooplanktivorous little auk Alle alle during the pre-laying period: insights from remote sensing and animal-tracking

BackgroundEnergy and time allocation in seabirds differ between consecutive stages of breeding given various requirements of particular phases of the reproductive period. Theses allocations may also be sex-specific considering differential energetic or nutritional requirements of males and females and/or sexual segregation in foraging niches and/or areas. In this study we investigated the foraging ecology of an Arctic, zooplanktivorous seabird, the little auk Alle alle during the pre-laying period using remote sensing of the environment and GPS-TDR loggers deployed on birds. We compared foraging trips range and habitats of birds with other stages of the breeding period and between sexes.ResultsWe found that little auks during the pre-laying period foraged exclusively in cold sea surface temperature zones (with temperatures < 5 ºC) but in various sea depth zones. They dived to similar depths ranging from -4.0 to -10.9 m, exploring various thermal microhabitats (with mean temperatures values ranging from 2.2 °C in Shelf sea depth zone to 5.9 °C in Deep sea depth zone). The majority of foraging trips and dives characteristics were similar to subsequent phases of breeding. However, home ranges during the pre-laying trips were wider compared to the incubation period. As expected, females exhibited wider foraging niches compared to males (wider range of sea surface temperature and sea depth in foraging locations), which could be explained by sex specific energetic and/or nutritional requirements (females producing an egg). We also delineated local foraging areas important for little auks during their whole breeding season. Protection of these areas is crucial for sustaining the local marine biodiversity.ConclusionsWe found that little auks females during the pre-laying period explored wider foraging niches compared to males. These differences may be attributed to sex-specific nutritional or/and energetical constraints at this stage of breeding. The results of this study also emphasize the importance of shelf Arctic-type water masses as the foraging areas for little auks during successive stages of breeding.

Growth monitoring and nutritional outcome of severe acute malnutrition children in the presence and absence of edema: a facility based retrospective study

Background: Severe acute malnutrition (SAM) is the leading cause of death in children. SAM affects approximately 20 million preschool children, mainly in Africa and South Asia. Children with severe acute malnutrition and severe edema (+++) have an increased risk of death compared with children with severe acute malnutrition and no edema. This study evaluated the nutritional outcome of SAM patients in the presence and absence of edema. Methods: This retrospective study was conducted in malnutrition treatment centre, JK Lon Hospital attached with Govt Medical College, Kota from January 2021 to December 2022 on children of age 6 months to 59 months, with clinical diagnosis of severe acute malnutrition. Ethics committee approval was not required as the study was a retrospective observational study. Patients were divided into three categories, viz. recovered, defaulted and non-responders. Results: A total of 270 patients were selected for this study on the basis of date made available through past records. Of the total patients studied, 32.2% (87) had bilateral edema while the rest (183) were non edematous. From edematous arm, 79.9% children recovered, 9.1% defaulted, and 11% were non-respondents. Conversely, of those without oedema 55.7% recovered, 7.0% defaulted, and 37.3% were non-respondents. Conclusions: Severe acute malnutrition is a preventable and treatable cause of childhood morbidity and mortality. The determinants of SAM include illiteracy, low socio-economic status, malnourished mother, low birth weight, ignorance about specific nutritional requirements of infants and young children, repeated infections, etc. In this study, we documented that the edematous children in the study area had a better likelihood of recovery as compared to those with severe wasting.

Effects of AM/PM Diets on Laying Performance, Egg Quality, and Nutrient Utilisation in Free-Range Laying Hens

Laying hen nutrient requirements change throughout the day, due to the cyclic nature of egg formation. Generally, more energy and protein are required in the morning when the albumen is deposited around the yolk, and more calcium is required in the evening when the eggshell is formed. The aim of this study was to determine if feeding laying hens diets tailored to their specific nutritional and physiological requirements throughout the day, by feeding higher levels of protein and energy in the morning and higher levels of calcium in the evening, is more beneficial than feeding one diet all day. Hy-Line Brown laying hens (n = 360) were housed in free-range floor pens (18 pens with 20 hens/pen) from 34 to 53 weeks of age (WOA). Half of the birds (n = 180, nine replicate pens) were fed a conventional layer hen diet all day (control) and the remaining birds (n = 180, nine replicate pens) were fed an AM diet from 08:00 h to 16:00 h and PM diet from 16:00 h to 08:00 h (AM/PM). From 39 WOA, hens were given access to an outdoor range from 09:00 h to 18:00 h via pop holes. Egg weight and hen-day egg production were measured daily, and feed consumption and the feed conversion ratio (FCR) were measured weekly. Hen weight and egg size uniformity were determined at 43 and 53 WOA, and egg quality was measured at 53 WOA. A total of 72 hens (4 hens/pen, 36 hens/treatment) were euthanised at 53 WOA to determine ileal apparent energy and nitrogen digestibility. A cost–benefit analysis for the study period, based on feed costs and egg mass, was calculated. Overall, the results showed that the AM/PM treatment increased egg mass by 2.15% (60.4 vs. 59.1 g/hen/day, p = 0.086) and improved feed efficiency by 8.34% (2.231 vs. 2.436 kg feed/kg egg, p = 0.030) compared with the control. A higher yolk colour score was observed in eggs from hens on the AM/PM treatment (p = 0.002), but no other significant effects of the treatments on egg quality were observed. Ileal digestible energy and digestible nitrogen coefficient were lower in hens on the AM/PM treatment compared with the control treatment (both p &lt; 0.001). However, the AM/PM treatment was attributed to a lower feed cost to egg mass compared with the control treatment (p &lt; 0.001). In conclusion, using an AM/PM feeding strategy was found to be economically beneficial.

The emerging role of medical foods and therapeutic potential of medical food-derived exosomes.

Medical food is consumed for the purpose of improving specific nutritional requirements or disease conditions, such as inflammation, diabetes, and cancer. It involves partial or exclusive feeding for fulfilling unique nutritional requirements of patients and is different from medicine, consisting of basic nutrients, such as polyphenols, vitamins, sugars, proteins, lipids, and other functional ingredients to nourish the patients. Recently, studies on extracellular vesicles (exosomes) with therapeutic and drug carrier potential have been actively conducted. In addition, there have been attempts to utilize exosomes as medical food components. Consequently, the application of exosomes is expanding in different fields with increasing research being conducted on their stability and safety. Herein, we introduced the current trends of medical food and the potential utilization of exosomes in them. Moreover, we proposed Medi-Exo, a exosome-based medical food. Furthermore, we comprehensively elucidate various disease aspects between medical food-derived exosomes (Medi-Exo) and therapeutic natural bionanocomposites. This review highlights the therapeutic challenges regarding Medi-Exo and its potential health benefits.

Genetic Engineering of Agrobacterium Increases Curdlan Production through Increased Expression of the crdASC Genes.

Curdlan is a water-insoluble polymer that has structure and gelling properties that are useful in a wide variety of applications such as in medicine, cosmetics, packaging and the food and building industries. The capacity to produce curdlan has been detected in certain soil-dwelling bacteria of various phyla, although the role of curdlan in their survival remains unclear. One of the major limitations of the extensive use of curdlan in industry is the high cost of production during fermentation, partly because production involves specific nutritional requirements such as nitrogen limitation. Engineering of the industrially relevant curdlan-producing strain Agrobacterium sp. ATTC31749 is a promising approach that could decrease the cost of production. Here, during investigations on curdlan production, it was found that curdlan was deposited as a capsule. Curiously, only a part of the bacterial population produced a curdlan capsule. This heterogeneous distribution appeared to be due to the activity of Pcrd, the native promoter responsible for the expression of the crdASC biosynthetic gene cluster. To improve curdlan production, Pcrd was replaced by a promoter (PphaP) from another Alphaproteobacterium, Rhodobacter sphaeroides. Compared to Pcrd, PphaP was stronger and only mildly affected by nitrogen levels. Consequently, PphaP dramatically boosted crdASC gene expression and curdlan production. Importantly, the genetic modification overrode the strict nitrogen depletion regulation that presents a hindrance for maximal curdlan production and from nitrogen rich, complex media, demonstrating excellent commercial potential for achieving high yields using cheap substrates under relaxed fermentation conditions.

Starting with Sustainability: Utilizing food Wastes as Nursery Feed for Black Solder Fly Neonates

With black soldier fly (BSF) larvae being recognized as effective waste convertors, the global market for BSF industry is projected to grow substantially in the coming decade. However, using non-waste materials in the bioconversion process increases the environmental footprint of the BSF industry [e.g.: chicken feed (CF) as primary neonatal nursery meal]. In this study, we first tested if industrial food wastes (i.e., okara, biscuit meal, dried fish, coconut.) can be substitute diets for BSF neonates. Next, we tested three formulated meals based on the nutritional properties of the well-performing nursery diets from the first test/previous literatures. In the first test, we observed significant differences in neonatal growth when food wastes were directly used as nursery meals, and the initial weight of neonates influenced the development time of the larvae, with smaller neonates exhibiting prolonged larval duration. In contrast, the formulated meals performed well in promoting the growth of neonates, and the larval development showed no significant difference by day eight in the post-nursery rearing. In summary, our findings indicate that by meeting specific nutritional requirements, formulated diets made from industrial food waste could serve as a viable replacement for CF as nursery meals, resulting in a significant boost in the sustainability of the BSF industry.Graphical

Establishment of a Single Temperature Incubation Approach for Environmental Monitoring Samples with Focus on Mold Recoveries.

A robust environmental monitoring program is essential to properly estimate and identify microorganisms in cleanrooms, ensuring that microbial contamination remains acceptably low, and a good state of control is maintained in the manufacturing areas. The incubation conditions are important to support optimal microbial recoveries, considering that there is no single culture medium, temperature and incubation time that can recover all microorganisms. Particularly molds are quite sensitive microorganisms, and some species may have very specific nutritional and environmental needs. In this study, a two-phase approach was used to identify a single incubation-temperature approach that could recover most of the cleanroom microbial flora with focus on molds. Phase 1 included a growth promotion study performed in the laboratory using pharmacopeial and in-house strains, comparing different media (SDA and TSA) at single or dual incubation-temperature approaches for 5 or 6 days. Phase 2 was based on an in situ study where sampling was performed in different areas of a pharmaceutical facility, comparing the recoveries at different incubation conditions. In addition, extension studies of phase 1 and phase 2 were performed to get a better understanding of growth requirements for in-house molds. The results show that an incubation on Tryptone Soy Agar (TSA) at 25-30° for 3-4 days was able to recover most tested microorganisms in phase 1 and a large variety of microorganisms in phase 2, indicating that the single incubation-temperature is an optimal approach for the recovery of microorganisms in cleanrooms. Exceptions were noted for one strain of the species Cutibacterium acnes, a microaerophilic bacterium for which anaerobiosis and higher temperatures were needed, and two mold strains (Sistotrema brinkmannii and Stereum hirsutum), indicating that those molds required a specific media (Sabouraud Dextrose Agar, SDA) for their proliferation. The results showed that TSA incubated at single or dual incubation-temperature approach cannot compensate for the absence of SDA for some environmental molds that may be atypical in cleanrooms. Therefore, in addition to TSA, certain monitoring with SDA at e.g., cleanroom entrance points may be beneficial to recover molds with very specific nutritional requirements.

A review of fermentation and the nutritional requirements for effective growth media for lactic acid bacteria

Lactic acid bacteria are useful microorganisms that are well-known to have probiotic effects and provide foods with unique sensory qualities such as aroma and taste (flavor). Probiotic bacteria such as Lactobacillus delbrueckii subsp. bulgaricus are found in many fermented food products and confer several human health benefits. Probiotic strains help to strengthen and boost the human immune system, increasing the body's resistance to a wide range of disease conditions. The food industry’s effort to meet customers' sensory and health demands in dairy and fermented food items has boosted the need for probiotic starter cultures with superior performance and health-beneficial qualities. One of the crucial dairy starter cultures for producing fermented dairy products such as yogurt and cheese is lactic acid bacteria, particularly L. bulgaricus. An enhanced fermentation media improves the generation of essential metabolites, such as lactic acid and the sensory attribute of fermented food. Therefore, this review aims to present an overview and the importance of lactic acid bacteria in fermentation. The review also presents information on specific nutritional requirements of growth media for fermentation purposes as well as new classifications and views on the present commercial applications of these healthy bacteria.

Investigating Owner Use of Dietary Supplements in Dogs with Canine Cognitive Dysfunction.

Canine cognitive dysfunction (CCD) is becoming increasingly recognized in veterinary medicine, as dogs live longer and with CCD being highly prevalent among the elderly dog population. Various studies have shown that diet and dietary supplementation can positively influence the clinical signs of CCD, especially if given at an early stage. The aim of this study was to investigate owner use of dietary supplements (DSs) in dogs with age-related behavioral changes. An observational study based on an online questionnaire for owners of dogs with age-related behavioral changes was performed. Out of a total of 394 owners who completed the survey, after noticing age-related behavioral changes, over half of the dogs received DSs (54%), whereas only 8% reported changing their dog's base diet. The most used DS was fish oil (48%). The use of DSs should be discussed with and monitored by veterinary surgeons since many geriatric patients have multi-morbidities, may have specific nutritional requirements and receive multi-faceted medications.

Debottlenecking and reformulating feed media for improved CHO cell growth and titer by data-driven and model-guided analyses.

Designing and selecting cell culture media along with their feeding are a key strategy to maximize culture performance in biopharmaceutical processes. However, the sensitivity of mammalian cells to their culture environment necessitates specific nutritional requirements for their growth and the production of high-quality proteins such as antibodies, depending on the cell lines and operational conditions employed. In this regard, previously we developed a data-driven and in-silico model-guided systematic framework to investigate the effect of growth media on Chinese hamster ovary (CHO) cell culture performance, allowing us to design and reformulate basal media. To expand our exploration for media development research, we evaluated two chemically defined feed media, A and B, using a monoclonal antibody-producing CHO K1 cell line in ambr15 bioreactor runs. We observed a significant impact of the feed media on various aspects of cell culture, including growth, longevity, viability, productivity, and the production of toxic metabolites. Specifically, the concentrated feed A was inadequate in sustaining prolonged cell culture and achieving high titers when compared to feed B. Within our framework, we systematically investigated the major metabolic bottlenecks in the tricarboxylic acid cycle and relevant amino acid transferase reactions. This analysis identified target components that play a crucial role in alleviating bottlenecks and designing highly productive cell cultures, specifically the addition of glutamate to feed A and asparagine to feed B. Based on our findings, we reformulated the feeds by adjusting the amounts of the targeted amino acids and successfully validated the effectiveness of the strategy in promoting cell growth, life span, and/or titer. This article is protected by copyright. All rights reserved.

Food exchange list based on macronutrients: adapted for the Ecuadorian population.

Food exchange lists allow health professionals to generate healthy eating plans adapted to individual or population needs. The objective of this study was to develop the first food exchange list based on the macronutrients and energy provided by the various food groups of the Ecuadorian diet. The list of Ecuadorian food exchanges was constructed by going through the following phases: (1) Selection of household measurements; (2) Selection of tables and databases of the nutritional composition of food items; (3) Definition of food groups and quantities; (4) Determination of the average energy and macronutrient values of each group; and (5) Photographic record. For the definition of food quantities, statistical criteria were applied according to a standard deviation of ±2SD; thus, for carbohydrates: ±5 g, total fat: ±2 g, and protein: ±3 g. To ensure the inclusion of the food items in the groups, a coefficient of variation of less than 30% and a Z value of ±2 were also considered. The list of food exchanges is presented with eight general groups according to the predominant nutrient (carbohydrates, proteins, or fats), and, where necessary, subgroups are included according to the second predominant nutrient. The list includes 404 food items with their photographic record, represented by their net weights and corresponding household measurement. All food items met the statistical criteria that help to reduce the variability of the nutritional composition of the food items in each group. This is the first list of Ecuadorian food exchanges based on statistical criteria. It represents a novel tool for public health professionals as well as researchers. Resulting healthier eating plans may improve daily dietetic practice, facilitate better clinical trial designs and help establish guidelines according to Ecuador's cultural and dietary patterns. The described methodology can further be used to develop other food exchanges lists for patients with specific nutritional requirements.

Unlocking the Power of Late-Evening Snacks: Practical Ready-to-Prescribe Chart Menu for Patients with Cirrhosis.

The efficacy of the late-evening snack (LES) has been extensively studied due to the impact of the longest intermeal duration occurring at night in patients with cirrhosis. While actual clinical guidelines on nutrition in chronic liver disease recommend an LES, no specific nutritional compositions have been reported by the European Association for the Study of the Liver (EASL) and the European Society for Clinical Nutrition and Metabolism (ESPEN). Late-evening snacks vary greatly among studies, including natural foods and/or nutritional supplements, yet oral supplements still need to fully meet the LES's nutritional composition. In addition, many hepatologists need to gain experience in nutritional approaches and have access to registered dieticians who can help them manage patients with liver disease. Therefore, this review study aims to summarise evidence regarding using LESs and the mechanisms behind long starvation in patients with cirrhosis. It also provides a practical nutritional guide with several LES options based on common natural foods tailored to special patients' nutritional requirements and geographical backgrounds. In preventing accelerated starvation and related protein malnutrition and sarcopenia in patients with cirrhosis, the nutritional composition of LESs is essential. The proper and straightforward application of the LES's rational nutrition is an advantage to cirrhotic patients and should be carried out by healthcare professionals to enhance the overall liver function and nutritional status of patients with cirrhosis.

Influence of Nutrition Knowledge on the Association between Maternal Nutrition and Birth Outcomes

Maternal undernutrition is a complex condition that arises from various factors, including social, cultural, psycho-social, biological, and ecological factors. The intergenerational consequences of chronic malnutrition, starting with mothers and their children, account for a significant proportion of infant deaths, cognitive disability, and reduced productivity in adulthood. Therefore, having nutritional knowledge throughout pregnancy is crucial for better risk assessment of undernutrition and healthy pregnancy outcomes. The study examined whether trimester-specific nutrition education and awareness could significantly impact the relationship between maternal nutrition and birth outcomes and found that this association was stronger in women with higher levels of nutrition knowledge. As a result, a food-based approach that is both low-cost and high-nutrition can help meet the specific nutritional requirements of pregnancy, alter certain nutrients that target fetal metabolic vulnerabilities, or enhance fetal growth and development in the migrant population. The review explores recent research and discusses how nutrition literacy and knowledge influence pregnancy and birth outcomes, providing an overview of the current understanding of maternal nutritional trimester-specific needs and highlighting areas that still require further study. The findings emphasize the importance of considering diet diversity and peer support during pregnancy, considering the impact that nutrition knowledge has on pregnancy outcomes.

Exploration of the nutritional and carotenoids profiles of vegetables in Thai cuisine as potential nutritious ingredients

Missing information on plant origin control and nutritional data on herbs, spices and vegetables could lead to sample quality deficit and misusage of the plant database. In this study, twenty vegetables that were collected and managed based on the recommendations of the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand, were investigated regarding their proximate mineral, vitamin and carotenoid contents using the standard procedures of the Association of Official Analytical Chemists (AOAC). The results showed that these plants (100 g dry weight) exhibited similar energy levels (337.11–420.48 kcal), which were mainly distributed from high carbohydrate content (21.01–88.17 g), while protein (3.14–66.07 g) and fat (0.00–10.33 g) levels were quite low. As a form of carbohydrate, dietary fiber was found to be high in Cymbopogon citratus (DC.) Stapf (Cy. citratus) and Solanum torvum Sw. (So. torvum) (57.00–59.54 g). Interestingly, Senegalia pennata subsp. insuavis (Lace) Maslin, Seigler & Ebinger (S. pennata) exhibited exceptionally high protein content, which was between 2.3 and 3.1 times higher than its carbohydrates. High mineral contents were detected in S. pennata, Ocimum africanum Lour. (O. africanum), Ocimum basilicum L. (O. basilicum), Ocimum gratissimum L. var. macrophyllum Briq. (O. gratissimum) and Coriandrum sativum L. (Co. sativum), while Mentha cordifolia Opiz ex Fresen (M. cordifolia) was observed to be a good source of vitamin C (381.36–547.47 mg). High carotenoids were mostly found in Eryngium foetidum L. (E. foetidum), O. gratissimum, Co. sativum and O. basilicum (75.23–119.96 mg). Interestingly, the location of sample collection seemed to have minimal effect on the nutritional and carotenoid compositions. The results of this study provide reliable information concerning the nutritional and carotenoid contents in plant sources with control of origin, which could be used in the future for food development with specific nutritional requirements.

Порівняльна характеристика різних способів збагачення грудного молока для дітей, які народилися передчасно. Що найкраще? (систематичний огляд)

Human milk is the preferred feeding for all infants, including those of very low birth weight and vulnerable groups. However, mother’s milk is generally likely has insufficient protein to promote appropriate growth. That's why fortification of human milk is required to meet nutrient requirements for growth and development for these preterm infants who are at high risk for growth faltering during the hospital stay. There are multiple strategies and products that may be employed to support desired growth rates. Purpose - Conduct comparative analysis the current literature on the fortification own mothers’ milk and influence of this fortification on the outcomes for preterm and sick infant’s health. Materials and methods. Search Strategy: A systematic search of the literature was conducted using search strategy was based on MeSH terms/subject headings and separate keywords. The study designs included were randomized or quasi-randomized controlled trials in the English, Ukrainian and Russian languages. Study Selection: Published literature was searched from January 2010 up to July, 1, 2022 using five databases (PubMed, Ovid Medline, Web of Science, Ovid Embase, and the Cochrane Library). The search resulted in 75 articles, and an additional article was identified by an expert in the field; 20 were used for review. Data Extraction: Data from the full-text articles was extracted into a Table. The manuscripts were organised in the table as follows: first Cochrane reviews and systematic reviews, followed by individual research studies that were not cited in any of the systematic reviews. Results and conclusions. The primary outcomes of this review were influence of different types fortification of mother's milk and time of starting fortification in short term growth parameters (length, head growth, and weight gain), feeding intolerance (defined as clinical signs only and/or cessation of feeding), length of hospital stay (number of days that the baby remained in the neonatal unit), and post menstrual age (i.e., gestational age plus chronological age) at discharge. Secondary outcomes were narcotising enterocolitis and sepsis. The study results suggested that early human milk fortification appears to positively affect growth for infants whose human milk feedings are fortified with a fortifier without adverse effects. Implications for Practice: Adequate nutrition in the early weeks of life is rarely achieved in premature infants, resulting in extrauterine growth restriction. The use of fortified human milk produces adequate growth in premature infants and satisfies the specific nutritional requirements of these infants. The use of individualised fortification is recommended but with proper protocols. No conflict of interests was declared by the authors.

Native and modified starches from underutilized seeds: Characteristics, functional properties and potential applications

Seeds represent a potential source of starch, containing at least 60–70% of total starch, however many of them are treated as waste and are usually discarded. The review aim was to analyze the characteristics, functional properties, and potential applications of native and modified starches from underutilized seeds such as Sorghum bicolor L. Moench (WSS), Chenopodium quinoa, Wild. (QSS), Mangifera indica L. (MSS), Persea americana Mill. (ASS), Pouteria campechiana (Kunth) Baehni (PCSS), and Brosimum alicastrum Sw. (RSS). A systematic review of scientific literature was carried out from 2014 to date. Starch from seeds had yields above 30%. ASS had the higher amylose content and ASS and RSS showed the highest values in water absorption capacity and swelling power, contrary to MSS and PCSS while higher thermal resistance, paste stability, and a lower tendency to retrograde were observed in MSS and RSS. Functional properties such as water solubility, swelling power, thermal stability, low retrogradation tendency, and emulsion stability were increased in RSS, WSS, QSS, and MSS with chemical modifications (Oxidation, Oxidation-Crosslinking, OSA, DDSA, and NSA) and physical methods (HMT and dry-heat). Digestibility in vitro showed that WSS and QSS presented high SDS fraction, while ASS, MSS, PCSS, and HMT-QSS presented the highest RS content. Native or modified underutilized seed starches represent an alternative and sustainable source of non-conventional starch with potential applications in the food industry and for the development of healthy foods or for special nutritional requirements.

Recomendaciones sobre la utilización de las fórmulas infantiles con proteína aislada de soya en la alimentación del lactante. Documento de posición basado en la evidencia

Soy infant formulas (SIF) have long been used as an alternative for infants with special nutritional requirements. However, the medical indications for their use, their allergenicity, safety and efficacy are still controversial. To present recommendations for the use of SIF based on the existing literature. We consulted systematic reviews and clinical practice guides, through validated search algorithms, and systems such as Appraisal of Guidelines for REsearch and Evaluation (AGREE)II and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). For the elaboration of the clinical recommendations, the most important topics were analyzed and discussed by the responsible researchers and the group of experts in the development of a position paper. The quality of the evidence was evaluated using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) system. Several reports with variable quality of evidence were found. They support SIF's efficacy in the management of cow's milk allergy and infant's digestive tolerance, as well as its low cross allergy compared to cow's milk and other formulas. We also found other studies that indicate the safety of SIFs on different nutritional, immune and developmental parameters. There is also evidence that demonstrates that SIFs have important advantages in terms of cost-benefit, palatability and effects on the intestinal microbiota, compared to other formulas. Although evidence to recommend its use in functional digestive disorders is limited, SIFs have an adequate safety profile and are still a valid option for infant feeding.