Specific Mode Research Articles

Effect of deformation mode on martensitic transformation in medium Mn steel

In the present work, we have investigated the effect of deformation modes namely uniaxial loading and plane strain loading on martensite transformation in medium Mn TRIP steels, since it plays an important role in influencing formability. Interrupted deformation experiments showed that the martensitic transformation proceeds continuously with plastic strain and is influenced by the specific deformation mode applied. Uniaxially loaded samples underwent a complete austenite to martensite transformation. On the other hand, under plane strain loading, only 70 % of the austenite transformed into martensite, even after fracture had occurred. Through synchrotron and EBSD experiments, we observed that under uniaxial loading, the transformation sequence followed the γ-ε-α′ pathway, whereas, under plane strain, a direct γ-α′ transformation was observed. The intermediate ε-martensite phase is known to facilitate the transformation from austenite to martensite. Consequently, the presence of ε-martensite resulted in a higher degree of austenite transformation in the uniaxial samples. We rationalize the difference in transformation sequences based on resolved shear stress calculations for the two deformation modes.

All-fiberized sorter for nondestructively splitting the orbital angular momentum modes

Orbital angular momentum (OAM) mode sorter is crucial for various OAM (de)multiplexing applications. However, it is challenging to sort the OAM mode without destroying its helical phase. Here, an all-fiber non-destructive OAM mode sorter is proposed for splitting the high purity (>90%) OAM beam with specific topological charge, polarization state and wavelength. By controlling the asymmetrical microfiber coupling region, only the specific OAM mode satisfies the phase matching condition. The all-fibered OAM sorter is optimized to splitting the third order OAM mode with the maximum coupling efficiency 91.02% and the purity 94.71%. The Multiple modes sorter can be realized through the multistage cascaded structure. The proposed all-fiber OAM sort will be a prospective component to expand the OAM beam fiber system.

Detecting Cracks on Plates Using Reflections of Fundamental Symmetric and Anti-symmetric Lamb Waves from Defect

Introduction: Ultrasonic Lamb waves can achieve long-range inspection on plate-like structures and can even detect unreachable cracks and defects. There are multiple modes under a certain generation frequency with most of them dispersive. Method: The dispersion curves of Lamb wave in a steel plate were given by numerical calculation. A 2-D finite element (FE) model of a steel plate was promoted. The fundamental symmetric and antisymmetric Lamb waves were generated by uploading symmetrical and anti-symmetrical displacements on one side of the plane. The wave reflects when it comes across the defect and the edge of the plate. Mode conversion will occur after the asymmetrical discontinuities of the structure. Result: The theoretical and numerical results of the values of group velocities of S0 and A0 in a steel plate show well agreement. The maximum amplitude of the converted A0 wave from the interaction of the incident S0 wave with the cracks was observed. The amplitude will first rise then fall under any depth of the defects. The relationship between the defect reflection and the length as well as the depth of defects were simulated based on the FE model. Phase reversion and phase delay were discovered by controlling the defect size changing in one dimension. The reflection coefficient increases linearly with the defect length while the depth is constant, as well as the defect depth while the length is constant. method: Using finite element (FE) method to simulate Lamb wave motion in a plate, as well as experiments on steel plates with artificial defects. Conclusion: Experiments on steel plates with artificial defects including thorough holes and tapered holes were carried out using ISONICA STAR. The results also show that when the defect size changes in one dimension such as the thorough holes, the reflection coefficient increases linearly correspondingly. However, the testing results of the taper holes were inconsistent with the regulation as the size increases in both the length and the depth. result: The values of group velocities were calculated by time-of-flight method to identify specific modes. The results were calibrated and showed well agreement with theoretical data. Mode conversion will appear when the wave comes across asymmetric discontinuities of the structure.

Comparative investigation on beam-to-beam connection with different types of connecting plates

The spliced glued laminated timber (glulam) beams have extensive applications in large-span timber structures since the length of the long solid wood beams is limited by transportation and construction. This paper investigated the flexural performance of glulam beam-to-beam connections with connecting plates and self-trapping screws. The influence of connecting plate types (steel plate, bamboo scrimber, and plybamboo plate) on the flexural performance of the spliced beams was studied. A total of 12 beams were designed, including the intact beam group (3 specimens) and the spliced beam group (9 specimens). The failure modes, ultimate bearing capacity, stiffness, moment-rotation curves, and carbon emissions of spliced beams with different connecting plates were studied. The test results showed that the failure mode of the spliced beams mainly included bending failure of glulam and fracture of the connecting plates. The average bearing capacity of the spliced beams with bamboo scrimber plates and those with steel plates could reach the intact beams. The average stiffness of the spliced beams with bamboo scrimber plates was higher than that of the splice beams with steel plates, reaching 97.6% of the intact beams. The average bearing capacity and stiffness of the splice beams with plybamboo plate reached 80.9% and 92.7% of the intact beams, respectively. A theoretical model of the spliced beams with bamboo scrimber plates was proposed and verified by tests data. Comparisons of the analytical and the test results reveal that the model can predict the moment capacity of the spliced beams with bamboo scrimber plates corresponding specific failure mode in this test.

A Novel Non-Destructive Rapid Tool for Estimating Amino Acid Composition and Secondary Structures of Proteins in Solution.

Amino-acid protein composition plays an important role in biology, medicine, and nutrition. Here, a groundbreaking protein analysis technique that quickly estimates amino acid composition and secondary structure across various protein sizes, while maintaining their natural states is introduced and validated. This method combines multivariate statistics and the thermostable Raman interaction profiling (TRIP) technique, eliminating the need for complex preparations. In order to validate the approach, the Raman spectra are constructed of seven proteins of varying sizes by utilizing their amino acid frequencies and the Raman spectra of individual amino acids. These constructed spectra exhibit a close resemblance to the actual measured Raman spectra. Specific vibrational modes tied to free amino and carboxyl termini of the amino acids disappear as signals linked to secondary structures emerged under TRIP conditions. Furthermore, the technique is used inversely to successfully estimate amino acid compositions and secondary structures of unknown proteins across a range of sizes, achieving impressive accuracy ranging between 1.47% and 5.77% of root mean square errors (RMSE). These results extend the uses for TRIP beyond interaction profiling, to probe amino acid composition and structure.

Does it matter how we ship the good apples out? On specific tariffs, transport modes, and agricultural export prices

AbstractFree‐on‐board (FOB) export prices for identical products from the same origin often differ across destinations, even when accounting for the trade costs and attributes of the destination country. One explanation for this observed price difference is per‐unit trade costs, and the ability of exporters to vary their markups and/or product quality. Using a novel dataset that details trade flows between countries by mode of transport, we estimate the transport mode‐specific effect of a per‐unit trade cost, specifically specific tariffs, on the FOB export prices of agricultural products. We find an elasticity of specific tariffs to export prices of 1.8%. However, the estimates are heterogeneous across modes of transport. The elasticity of specific tariffs to export prices is 2% for air transport, 5% for road transport, and .3% for sea cargo. Since the observed positive export price effect can reflect product quality differences or markups, we account for the quality element and find that for a given product quality, markups increase with increasing specific tariffs. This form of price discrimination is less pronounced for higher‐quality products that are predominantly shipped by air.

Examining the impact and response to an outbreak of carbapenemase-producing Enterobacterales in a neonatal unit in the United Kingdom: An outbreak report.

Carbapenemase-producing Enterobacterales (CPE) are a group of Gram-negative bacteria causing global concern due to their resistance to carbapenems. In this report, we detail the learning points from a CPE outbreak in a tertiary neonatal unit (NU) in the UK. Routine surveillance screening (rectal swabs) of babies on the NU identified a potential cluster of CPE carriage. Samples were sent to a reference laboratory for confirmatory testing. Environmental screening and cot mapping were undertaken to determine movements of babies within the unit. Regular audits of cleaning standards, hand hygiene, and maternal hygiene when expressing breast milk were carried out. The outbreak lasted 19weeks. During the outbreak, there were 360 admissions, with 11 babies being colonised with the outbreak strain. Once the outbreak was declared, there were enhanced Infection Prevention and Control (IPC) precautions (including increased environmental and equipment cleaning frequency). CPE screening frequency was increased and cot capacity was reduced. Hand hygiene compliance improved from 92% at the start of the outbreak to 100% by its close. Cleaning standards remained compliant. Maternal hygiene standards varied from 78% to 100%, but no cross-infection links were identified. Environmental screening was negative. No route of cross-infection was identified. Notably, no babies developed invasive CPE infection. This is the first report of a CPE outbreak in a UK NU. Although no specific mode of cross-transmission was identified and the outbreak's end cannot be attributed to any single intervention, the bundle of interventions proved successful after a 5-month period.

Density Functional Theory Calculation May Confirm Arsenic-Thiol Adhesion as the Primary Mechanism of Arsenical Toxicity.

Previously, it was believed that methylation was the body's primary method to detoxify inorganic arsenic. However, recent research has shown that the metabolized intermediate known as MMAIII is more toxic than arsenite and arsenate, contradicting a previous understanding. Another important question arises: is arsenical toxicity truly caused by arsenic binding to proteins through arsenic thiol adhesion? Based on the toxicity order of the experiment, with MMAIII being the most toxic, followed by arsenite, arsenate, DMAV, and MMAV, density functional theory (DFT) calculations can provide a straightforward assessment of this issue. Our practice captures all the transition states associated with a specific imaginary-frequency vibration mode, including proton transfer and simultaneous departure of leaving group. We have obtained the energy barriers for five arsenicals reacting with thiol, alcohol, and amine separately. In addition to energetic favorability, the following are the energy barriers for arsenic's reaction with thiol ranked from low to high: MMAIII (25.4 kcal/mol), arsenite (27.7 kcal/mol), arsenate (32.8 kcal/mol), DMAV (36.2 kcal/mol), and MMAV (38.3 kcal/mol). Results show that the toxicity of arsenicals is mainly caused by their reaction with thiol rather than with alcohol or amine, as supported by the trend of decreasing toxicity and increasing energy barriers. Thus, this DFT calculation may confirm the paradigm that arsenic-thiol adhesion is the primary cause of arsenic toxicity in the body.

Systematic Review of Household Transmission of Strep A: A Potential Site for Prevention That Has Eluded Attention.

Although Streptococcus pyogenes (Strep A) is the sixth-most common infectious disease globally, its transmission within the household remains an understudied driver of infection. We undertook a systematic review to better understand the transmission of Strep A among people within the home, while highlighting opportunities for prevention. A search strategy was applied to 5 databases between September 2022 and March 2023. Results were limited to articles published between January 2000 and March 2023. Texts were reviewed by 2 authors and the following data extracted: article details (title, author, year), study type, transmission year, country, participant age, infection status, molecular testing, and transmission mode. Funding was provided by the Australian National Health and Medical Research Council (GNT2010716). The final analysis comprised 28 texts. Only 7 studies (25.0%) provided sufficient detail to identify the Strep A transmission mode: contact (n = 4), vehicle (bedding, clothing, other fabric, and medical equipment; n = 2), and contact with animals (n = 1). All others were classified as household (specific mode unascertainable). Most articles reported outbreaks involving invasive Strep A infections. There is limited literature regarding household transmission of Strep A. Understanding transmission in this setting remains imperative to guide control methods.

The impact of water rights trading on water resource use efficiency: Evidence from China's water rights trading pilots

With the increasing conflict between the supply and demand of water resources, enhancing water resource use efficiency has gradually become a focus of attention. As a new way to manage water resources, water rights trading pilots have been implemented in China. It is important to explore the impact of water rights trading on regional water resource use efficiency and possible impact paths. This paper employs a global nonradial directional distance function (GNDDF) model to measure water resource use efficiency, which is more accurate and reliable. To discern the effect of China's water rights trading pilot policy on enhancing regional water resource use efficiency, we apply the regression control method (RCM) for counterfactual analysis and test the possible pathways through which the water rights trading policy affects water resource use efficiency. This article draws the following conclusions. (i) China's overall water resource use efficiency has not reached a high level over the past decade, and water resource use efficiency has exhibited substantial regional disparities. (ii) There is some evidence to suggest that the water rights trading policy may have a positive impact on improving water resource use efficiency in the pilot provinces. However, the improvement effect exhibits regional variations, which are likely attributed to the initial water resource use levels in the pilot provinces and the specific implementation modes of water rights trading. (iii) Water rights trading policies can enhance water resource use efficiency through two pathways, including facilitating reclaimed water reuse and promoting water use restructuring.

Shift of ambidexterity modes: An empirical investigation of the impact of artificial intelligence in customer service

To manage the trade-offs between exploration and exploitation, customer service organizations typically choose a specific ambidexterity mode, such as structural separation or behavioral integration, that suits their context. However, recent research suggests that organizations need to dynamically change their ambidexterity modes to better align with changing environmental and organizational contexts. This study examines the impact of AI in facilitating the shift across various modes of ambidexterity in customer service organizations. Our findings reveal that achieving shifts in ambidexterity modes is challenging, and organizations must overcome technology, business process, and stakeholder related barriers. Based on our findings, we develop a set of propositions for further investigation. Our study contributes to research on the dynamic perspective of ambidexterity by shedding light on how customer service organizations shift from one ambidexterity mode to another and by articulating the important roles played by AI in achieving such shifts.

Removal of Interference from Electromyogram based on Empirical Mode Decomposition and Correlation Coefficient Thresholding

Introduction:: Electromyography (EMG) signals are contaminated by various noise components. These noises directly degrade the EMG processing performance, thereby affecting the classification accuracy of the EMG signals for implementing various hand movements of the prosthetic arm from the amputee’s residual muscle. Methods:: This study mainly aims to denoise the EMG signals using the empirical mode decomposition (EMD) and correlation coefficient thresholding (CCT) methods. The noisy EMG signal is obtained from NinaPro Database 2. Then, EMD is used to decompose it into intrinsic mode functions. Each hand movement noise is identified within specific modes and removed separately using correlation coefficient–dependent thresholding and wavelet denoising. The performance metrics signal-to-noise ratio (SNR) and root mean square error (RMSE) were used to evaluate the noise removal performance from the EMG signals of five intact subjects. The proposed method outperforms the wavelet denoising method in terms of noise interference removal. In this method, the SNR is obtained in the 17-22 dB range with a very low RMSE. Results:: The experimental results illustrate that the proposed method removes noise from six repetitions of six movements performed by five subjects. This study explores the special characteristics of EMD and demonstrates the possibility of using the EMD-based CCT filter for denoising EMG signals. Conclusion:: The proposed filter is more efficient than wavelet denoising in removing noise interference. It can also be used in any application that requires EMG signal filtering at the preprocessing stage, such as EMG pattern recognition.

Active stabilization of an open-access optical microcavity for low-noise operation in a standard closed-cycle cryostat

Open-access optical microcavities are Fabry–Perot type cavities consisting of two micrometer-size mirrors, separated by an air (or vacuum) gap typically of a few micrometers. Compared to integrated microcavities, this configuration is more flexible as the relative position of the two mirrors can be tuned, allowing for easy changes in parameters such as cavity length and mode volume and the selection of specific transverse cavity modes. These advantages come at the expense of the mechanical stability of the cavity itself, which is particularly relevant in noisy closed-cycle cryostats. Here, we show an open-access optical microcavity based on scanning-probe microscope design principles. When operated at 4 K in a tabletop optical closed-cycle cryostat without any dedicated mechanical low-pass filter, we obtain stabilities of 5.7 and 10.6 pm rms in the quiet and full periods of the cryocooler cycle, respectively. Our device has free-space optical access, essential, for instance, for full polarization control.

Research on optimization of supply chain inventory system under contingency conditions

A solution combining state observer (SOB) with Discrete Sliding Mode Control (DSMC) is presented to solve the problem of inventory shortage or backlog in upstream and downstream enterprises of the supply chain caused by contingency conditions. Firstly, based on the specific sales mode of the supply chain system and the logical relationship of inventory parameters, the corresponding dynamic model of the inventory system is established under reasonable assumptions, and relevant parameters are introduced to represent the impact of contingencies, and the dynamic model of inventory system under contingencies is established with the bullwhip effect. Then, the discrete sliding mode control (SOB-DSMC) based on the state observer is designed to compensate for the impact of emergencies on the supply chain inventory system adaptively while ensuring the stability of the system. Finally, based on market research and reasonable assumptions, simulation experiments are carried out. The simulation results show that the designed control system can better cope with emergencies in different situations, and can significantly improve the supply chain within 5 days after emergencies occur. The inventory situation of upstream and downstream enterprises in the system has reduced the maximum inventory fluctuations of manufacturers, distributors, and retailers by 77.13%, 60.67%, and 44.61% respectively, and fully restored to normal operation within 20 days, effectively solving the inventory backlog of upstream and downstream enterprises in the supply chain and out-of-stock issues. It can be seen that the SOB-DSMC method can guide the upstream enterprises in the supply chain to formulate reasonable production, shipping, and purchasing strategies, and provide theoretical reference and coping methods for the supply chain system to smoothly survive emergencies.

Analysis of the inter-domain orientation of tandem RRM domains with diverse linkers: connecting experimental with AlphaFold2 predicted models.

The RNA recognition motif (RRM) is the most prevalent RNA binding domain in eukaryotes and is involved in most RNA metabolism processes. Single RRM domains have a limited RNA specificity and affinity and tend to be accompanied by other RNA binding domains, frequently additional RRMs that contribute to an avidity effect. Within multi-RRM proteins, the most common arrangement are tandem RRMs, with two domains connected by a variable linker. Despite their prevalence, little is known about the features that lead to specific arrangements, and especially the role of the connecting linker. In this work, we present a novel and robust way to investigate the relative domain orientation in multi-domain proteins using inter-domain vectors referenced to a stable secondary structure element. We apply this method to tandem RRM domains and cluster experimental tandem RRM structures according to their inter-domain and linker-domain contacts, and report how this correlates with their orientation. By extending our analysis to AlphaFold2 predicted structures, with particular attention to the inter-domain predicted aligned error, we identify new orientations not reported experimentally. Our analysis provides novel insights across a range of tandem RRM orientations that may help for the design of proteins with a specific RNA binding mode.

Hijacking and rewiring of host CircRNA/miRNA/mRNA competitive endogenous RNA (ceRNA) regulatory networks by oncoviruses during development of viral cancers.

A significant portion of human cancers are caused by oncoviruses (12%-25%). Oncoviruses employ various strategies to promote their replication and induce tumourigenesis in host cells, one of which involves modifying the gene expression patterns of the host cells, leading to the rewiring of genes and resulting in significant changes in cellular processes and signalling pathways. In recent studies, a specific mode of gene regulation known as circular RNA (circRNA)-mediated competing endogenous RNA (ceRNA) networks has emerged as a key player in this context. CircRNAs, a class of non-coding RNA molecules, can interact with other RNA molecules, such as mRNAs and microRNAs (miRNAs), through a process known as ceRNA crosstalk. This interaction occurs when circRNAs, acting as sponges, sequester miRNAs, thereby preventing them from binding to their target mRNAs and modulating their expression. By rewiring the host cell genome, oncoviruses have the ability to manipulate the expression and activity of circRNAs, thereby influencing the ceRNA networks that can profoundly impact cellular processes such as cell proliferation, differentiation, apoptosis, and immune responses. This review focuses on a comprehensive evaluation of the latest findings on the involvement of virus-induced reprogramming of host circRNA-mediated ceRNA networks in the development and pathophysiology of human viral cancers, including cervical cancer, gastric cancer, nasopharyngeal carcinoma, Kaposi's sarcoma, hepatocellular carcinoma, and diffuse large B cell lymphoma. Understanding these mechanisms can improve our knowledge of how oncoviruses contribute to human tumourigenesis and identify potential targets for developing optimised therapies and diagnostic tools for viral cancers.

Single-mode nanolasers based on FP-WGM hybrid cavity coupling

As an idealized light source, semiconductor nanowire (NW) lasers have been extensively studied due to its potential applications in many fields such as optoelectronics, nanophononics, optical communication, signal processing, and displays. In this letter, we proposed a novel approach to realize a single-mode nanolaser by forming an Fabry–Perot whispering gallery mode (FP-WGM) hybrid nanocavity between two cross-contact CdS NWs, i.e. x and y-NW. In our method, x-NW supports the regular FP oscillation in the axis direction while the cross section of y-NW provides a ultrasmall WGM nanocavity with a higher Q-factor and mode election which confirms the specific single mode can be excited. Experimentally, single-mode lasing emission centered at 517 nm was obtained with full width at half maximum of 0.08 nm and lasing threshold of ∼50 kW cm−2. The suggested designing skills projected a general strategy for lasing mode regulation and single-mode realization. The single-mode low-threshold lasing strategy in coupled NWs may open a new avenue for practical applications of NW lasers and further trigger other photonic devices at a visible range.

Securing Supply Chains in Open Source Ecosystems: Methodologies for Determining Version Numbers of Components Without Package Management Files

In the case of supply chain security detection research, determining the component version number is a crucial task for the open source components of package-free management files. This paper aims to explore the new perspective of the determination of component version numbers based on various methods and to propose an effective method. First, by analyzing the source code of the component, you can try to determine the version number of the component by a specific mode, function, or variable in the code. This approach requires in-depth study and analysis of the source code to extract key code snippets that may contain version information. Second, the submission history of the component can be used to track the change of the version number. The modification content and update information for each version is obtained by viewing the submission records of the components in the version control system. Such an approach is relatively feasible for those components with a canonical versioning history. In addition, the metadata or metadata information of the component can be used to determine the version number. Some open-source components may contain version-related information in their code or documentation, such as release date, release instructions, version labels, etc. By parsing and extraction of these metadata, the version number of the components is obtained. In addition, the version number of the component can be obtained through communication with the community or the developer. Participate in the relevant open source community or contact component developers to consult them for information about the component version. This approach may require more time and resources, but is a viable option for those components that are difficult to determine the version number through other means. To sum up, the determination of the version number of open source components without package management files is an important link in supply chain security detection.

Characteristics of Hepatitis B Virus, Hepatitis C Virus, and Syphilis Coinfection in People With HIV/AIDS Contracted Through Different Sources: Retrospective Study.

The burden of hepatitis B virus (HBV), hepatitis C virus (HCV), and syphilis coinfections remains disproportionately high among people living with HIV/AIDS. Hubei province is located in central China, where there are distinct regional characteristics of the distribution of people living with HIV/AIDS acquired via diverse transmission routes and the AIDS epidemic itself. We aimed to estimate the magnitude of HBV, HCV, or syphilis coinfections among people living with HIV/AIDS with blood-borne transmission, which includes former paid blood donors, contaminated blood recipients, and intravenous drug users, as well as among people with sex-borne HIV transmission (including heterosexual people and men who have sex with men) and people with mother-to-child HIV transmission. From January 2010 to December 2020, people living with HIV/AIDS were tested for hepatitis B surface antigen (HBsAg), HCV antibodies, and syphilis-specific antibodies. The positive patients were further tested for HBV markers, HBV DNA, and HCV RNA, and received a rapid plasma reagin circle card test. All people living with HIV/AIDS were first divided into transmission groups (blood, sex, and mother-to-child); then, people with blood-borne HIV transmission were divided into former paid blood donors, contaminated blood recipients, and intravenous drug users, while people with sex-borne HIV transmission were divided into heterosexual people and men who have sex with men. Among 6623 people living with HIV/AIDS, rates of chronic HCV infection were 80.3% (590/735) in former paid blood donors, 73.3% (247/337) in intravenous drug users, 57.1% (444/777) in contaminated blood recipients, 19.4% (21/108) in people with mother-to-child HIV transmission, 8.1% (240/2975) in heterosexual people, and 1.2% (21/1691) in men who have sex with men. Chronic HBV infection rates were similar among all people with blood-borne HIV transmission. However, compared to heterosexual people, the chronic HBV infection rate was greater in men who have sex with men (213/1691, 12.6% vs 308/2975, 10.4%; χ21=5.469; P=.02), although HBV exposure was less common (827/1691, 48.9% vs 1662/2975, 55.9%; χ21=20.982; P<.001). Interestingly, the combination of HBsAg and hepatitis B e antigen (HBeAg) was found in 11 patients with sex-borne HIV transmission, but in 0 people with blood-borne HIV transmission (11/196, 5.6% vs 0/521, 0%; χ21=29.695, P<.001). In people with sex-borne HIV transmission, the proportions of patients with a syphilis titer ≥1:16 and neurosyphilis were 8.6% (105/1227) and 7.8% (37/473), respectively, whereas these values were 0 in people with blood-borne HIV transmission. In people living with HIV/AIDS, HCV transmission intensity was significantly associated with specific exposure modes of blood or sexual contact. The rate of chronic HBV infection among men who have sex with men was higher than in any other population. Attention should be paid to the high prevalence of neurosyphilis in people living with HIV/AIDS who contract HIV by sexual intercourse.

Safety and feasibility of a functional electrical stimulation cycling-based muscular dysfunction diagnostic method in mechanically ventilated patients.

A nonvolitional diagnostic method based on FES-Cycling technology has recently been demonstrated for mechanically ventilated patients. This method presents good sensitivity and specificity for detecting muscle dysfunction and survival prognosis, even in unconscious patients. As the clinical relevance of this method has already been reported, we aimed to evaluate its safety and feasibility. An observational prospective study was carried out with 20 critically ill, mechanically ventilated patients. The FES-cycling equipment was set in a specific diagnostic mode. For safety determination, hemodynamic parameters and peripheral oxygen saturation were measured before and immediately after the diagnostic protocol, as well as venous oxygen saturation and blood lactate. The creatine phosphokinase level (CPK) was measured before and 24, 48, and 72 h after the test. The time taken to carry out the entire diagnostic protocol and the number of patients with visible muscle contraction (capacity of perceptive muscular recruitment) were recorded to assess feasibility. Heart rate [91 ± 23 vs. 94 ± 23 bpm (p = 0.0837)], systolic [122 ± 19 vs. 124 ± 19 mm Hg (p = 0.4261)] and diastolic blood pressure [68 ± 13 vs. 70 ± 15 mm Hg (p = 0.3462)], and peripheral [98 (96-99) vs. 98 (95-99) % (p = 0.6353)] and venous oxygen saturation [71 ± 14 vs. 69 ± 14% (p = 0.1317)] did not change after the diagnostic protocol. Moreover, blood lactate [1.48 ± 0.65 vs. 1.53 ± 0.71 mmol/L (p = 0.2320)] did not change. CPK did not change up to 72 h after the test [99 (59-422) vs. 125 (66-674) (p = 0.2799) vs. 161 (66-352) (p > 0.999) vs. 100 (33-409) (p = 0.5901)]. The time taken to perform the diagnostic assessment was 11.3 ± 1.1 min. In addition, 75% of the patients presented very visible muscle contractions, and 25% of them presented barely visible muscle contractions. The FES cycling-based muscular dysfunction diagnostic method is safe and feasible. Hemodynamic parameters, peripheral oxygen saturation, venous oxygen saturation, and blood lactate did not change after the diagnostic protocol. The muscle damage marker (CPK) did not increase up to 72 h after the diagnostic protocol.