Specific Migration Research Articles

Dynamic Monitoring of Haze Pollution Using Satellite Remote Sensing

The severe haze pollution episode from December 1 to 10, 2013 occurred in Shanghai area, and seriously threatened human health and transportation safety. As regional air pollution, it is practical significance to the rapid monitoring and comprehensive analysis for haze treatment. Based on the moderate resolution imaging spectro- radiometer (MODIS) and cloud-aerosol LiDAR with orthogonal polarization (CALIOP) data, via combing with meteorological features during the haze pollution period, the current study analyzed and explored the spatial distribution, aerosol vertical structure, source and migration path of haze pollution. The results showed that: (1) There are obvious aerosol layers over Shanghai area, aerosol optical depth (AOD) fluctuates frequently and has distinctive features such as complexity, regionality and seasonality. (2) The aerosol layer is mainly at the heights ranging from 0 to 2 km and composed mostly of spherical aerosol but there is also non-spherical aerosol. (3) The total backscattering intensity of aerosol is centralized at the heights ranging from 0 to 1 km, especially at the heights ranging from 0 to 0.5 km. (4) The haze pollution episode was affected by both specific meteorological factors and migration of the non-local particles in the surrounding areas and northern China.

Click CAR-T cell engineering for robustly boosting cell immunotherapy in blood and subcutaneous xenograft tumor

The adoptive transfer of chimeric antigen receptor-T (CAR-T) cells has shown remarkable clinical responses in hematologic malignancies. However, unsatisfactory curative results and side effects for tumor treatment are still unsolved problems. Herein we develop a click CAR-T cell engineering strategy via cell glycometabolic labeling for robustly boosting their antitumor effects and safety in vivo. Briefly, paired chemical groups (N3/BCN) are separately incorporated into CAR-T cell and tumor via nondestructive intrinsic glycometabolism of exogenous Ac4GalNAz and Ac4ManNBCN, serving as an artificial ligand-receptor. Functional groups anchored on cell surface strengthen the interaction of CAR-T cell and tumor via bioorthogonal click chemistry, further enhancing specific recognition, migration and selective antitumor effects of CAR-T cells. In vivo, click CAR-T cell completely removes lymphoma cells and minimizes off-target toxicity via selective and efficient bioorthogonal targeting in blood cancer. Surprisingly, compared to unlabeled cells, artificial bioorthogonal targeting significantly promotes the accumulation, deep penetration and homing of CAR-T cells into tumor tissues, ultimately improving its curative effect for solid tumor. Click CAR-T cell engineering robustly boosts selective recognition and antitumor capabilities of CAR T cells in vitro and in vivo, thereby holding a great potential for effective clinical cell immunotherapy with avoiding adverse events in patients.

Topical formulations containing aptamer-functionalized nanocapsules loaded with 5-fluorouracil - An innovative concept for the skin cancer therapy

New topical gel formulations based on sodium alginate and hyaluronic acid containing AS1411 aptamer-functionalized polymeric nanocapsules loaded with an antitumoral drug (5-Fluorouracil) were designed as an innovative approach for the skin cancer treatment. Several important analyses were used to characterize these obtained topical gel formulations, namely: rheological tests, permeation assays across Strat-M® artificial membrane, ex-vivo permeation assays across chicken skin membrane, haemolysis tests, skin irritation tests, in vitro cytotoxicity assay on human basal carcinoma cells and in vivo tests. Rheological tests revealed that apparent viscosity decreases with the increase of the shear rate, for analyzed samples, which demonstrates a shear thinning behavior. Low levels of hemolysis values which ranged between 0.03 and 0.55% suggested that the tested formulations did not induce red blood cell lysis.. The gel formulations containing nanocapsules loaded with 5-FU proved to be non-irritant. Furthermore, by study the ex-vivo diffusion properties across the chicken skin membrane, it was proved that nanoencapsulation enhance the permeability properties of 5-FU. In vitro cytotoxicity assay on TE 354.T (ATCC® CRL-7762™) human basal carcinoma cell line showed that the obtained formulations loaded with 5-Fluorouracil manifest an important cytotoxic effect. Finally, the presence of Langerhans CD68 cells-positive in the epidermis and epithelial sheath of dermal hair follicles suggests a specific activation, migration and retrieval of nanoparticles by these cells. Following the results obtained in this study we can appreciate that the obtained topical gel formulations have a favourable biosafety and good antitumor effects which makes them attractive for skin cancer treatment.

LC-MS/MS analysis of BADGE, NOGEs, and their derivatives migrated from food and beverage metal cans

ABSTRACT A simple and novel method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated to determine the levels of 10 compounds (bisphenol A diglycidyl ether (BADGE), novolac-glycidyl ethers (NOGEs), and their related compounds) migrated from food and beverage cans into food simulants. Method validation showed acceptable linearity, precision, and accuracy. The detection limits ranged from 0.28 to 14.8 μg L−1, and the quantification limits ranged from 0.94 to 49.3 μg L−1. Water, 4% acetic acid, 50% ethanol, and n-heptane were employed as food simulants for the migration tests, and the developed LC-MS/MS method was applied to 104 epoxy-coated beverage and food metal cans. Only BADGE∙2H2O and BADGE were detected; the levels were below the specific migration limit. Based on the obtained migration results, the estimated daily intakes (EDIs) of BADGE∙2H2O and BADGE were calculated. Exposure assessments were conducted to compare the EDI with the tolerable daily intake (TDI), with a relatively low percentage of the TDI being reported. NOGE and its related compounds were not detected in the monitored cans. Long-term storage tests were also conducted at 60°C over 30 days. Only BADGE∙2H2O was detected in all food simulants, except for n-heptane, and the maximum amount detected was 114.6 μg L−1 in 50% ethanol.

Plasticisers and preservatives in commercial milk products: A comprehensive study on packages used in the Spanish market

A comprehensive study on packaging used in commercially available milk products from Spanish markets has been presented. Concentrations of four phthalates, seven parabens and BPA were determined in forty-two milk products. Eleven brands and five types of packaging (metallic aluminium bag, carton, high-density polyethylene, metal pail and polyethylene terephthalate) were included in the study. BPA showed the lowest concentrations (8.3 pg/g f.w.), far below those of phthalates (6431 pg/g f.w.) and parabens (6234 pg/g f.w.). Metallic aluminium bags were the least migrating packaging (considering plasticisers and monomers) followed by HDPE bottles, in the case of phthalates. Parabens showed their highest concentrations for fresh-milk samples. Levels found were far below the specific migration limits established by the EU and the cumulative hazard index was lower than 1, indicating that adverse health effects were not expected. In general, the results found in Spanish samples were lower than those reported in other countries.

Changes of thermal properties and microstructure of nano‐ZnO/polylactic acid composite films during Zn migration

In this study, polylactic acid (PLA) films with different nano‐ZnO concentration (0, 1, 3, 5, 7, 9, and 15%wt) were obtained by casting method. Overall migration values and specific migration values were studied in different food simulants (10% [v/v] ethanol and isooctane). The results demonstrated that overall migration of the composite films and specific migration of Zn2+ was higher in 10% (v/v) ethanol solution than in isooctane solution. The release of Zn2+ was affected by the percentage of nano‐ZnO in the composite films and food simulants. The migration of Zn in this study was mainly caused by polymer softening and degradation leading to the dissolution of Zn. Scanning electron microscope analysis indicated that with the migration of Zn2+, the structure of the composite films changed greatly. Differential scanning calorimeter analysis indicated that the thermal stability was decreased and the glass transition temperature and crystallization temperature were reduced.

Colliding in the Shadows of Giants: Planetesimal Collisions during the Growth and Migration of Gas Giants

Abstract Giant planet migration is an important phenomenon in the evolution of planetary systems. Recent works have shown that giant planet growth and migration can shape the asteroid belt, but these works have not considered interactions between planetesimals. We have calculated the evolution of planetesimal disks, including planetesimal–planetesimal collisions, during gas giant growth and migration. The numbers, locations, and impact velocities of these collisions depend on the specific growth and migration path. We find that giant planet growth alone has little effect on impact velocities, and most of the planetesimals scattered by growing giants do not undergo collisions. In contrast, we find that giant planet migration induces large numbers of high-velocity collisions between planetesimals. These impacts have sufficient velocities to cause shock-induced vaporization for both water ice and silicate components of planetesimals and to cause disruption of the bodies. New bodies may form from impact debris. Collisional evolution reduces the efficiency of planetesimal implantation into the asteroid belt. A small fraction of the largest planetesimals implanted into the asteroid belt would have been processed via collisions. We identify important consequences of planetesimal collisions that have not been considered in planet accretion models. The prevalence of high-velocity collisions during giant planet migration, and their potential links to the properties of meteorites and the formation of chondrules, makes impact vaporization a critically important phenomenon. The consequences of vaporizing planetesimal constituents require further detailed study. New collision outcome models for impacts within the nebula, and models for new planetesimal formation are needed.

Release of volatile compounds from cooking plastic bags under different heating sources

Abstract The use of packaging materials at very high temperatures, such as in-pack cooking, could lead to the formation of odorous compounds and also to the transference of migrants to food. In this work, the effect of high temperature or microwave cooking has been studied for 2 different cooking bags. The study was performed by gas-chromatography coupled to mass spectrometry and to olfactometric detection (GC-MS-O). The results showed that the bags heated in a conventional oven had a higher effect on the increment of odor impact compounds from the packaging than those heated in microwaves. Aldehydes and ketones were the major responsible for the odors detected in the olfactometry. Migration experiments to different food simulants (ethanol 10%, vegetable oil and ethanol 95%) and food samples (chicken) were also performed. In migration to food simulants, migrants were only detected in ethanol 10 %: 1-nonanol was detected below the specific migration limit established in the European Regulations and nonanal and decanal were below 10 ng/g. In migration to chicken a total of 27 compounds, mostly aldehydes, were transferred to it under the worst case migration conditions.

Abstract 4917: Estrogen receptor D538G mutation promotes cell migration via hyperactivation of Wnt signaling pathway

Abstract Background: Hotspot estrogen receptor-α (ERα/ESR1) mutations occur in 30-40% endocrine resistant ER+ breast cancer, with Y537S and D538G as the two most frequent hotspot mutations. A mostly unanswered question is and how these mutations facilitate metastatic processes. Methods: Frequencies of different ESR1 hotspot mutations were compared within three publicly available cohorts. Y537S and D538G genome-edited MCF7 and T47D cell models were used for in vitro characterization. Wound scratching, spheroid collective migration, chemotaxis assays were applied to examine different metastatic properties. Tail vein injection in nude mice followed by human CK19 lung section immune staining was used to characterize in vivo metastasis. Canonical Wnt pathway activity was studied using top-flash reporter assays, immunoblotting, and overexpression of dominant negative TCF4. ChIP-seq and ATAC-seq were utilized to profile ER global binding patterns and chromatin accessibility in cell models, respectively. Porcupine inhibitor-LGK974 was used to evaluate the efficiency of Wnt-targeted therapy. Results: D538G mutations were more frequent in ER+ metastatic lesions compared to Y537S among three metastatic breast cancer cohorts with unbiased SNV detection. In line with this, T47D-D538G ESR1 mutant cells showed strongly enhanced cell migration in vitro which was not observed in Y537S cells. We also observed increased lung micro-metastasis in vivo after tail vein injection of the D538G cells. Cell line transcriptomic analysis revealed uniquely hyperactivated Wnt pathway in D538G mutant cells, which was further confirmed in vitro by top-flash reporter and immunoblot. Suppression of canonical Wnt pathways blocked T47D-D538G specific cell migration. Combination treatment of fulvestrant and LGK974 synergistically inhibited D538G specific migration. Mechanistically, multiple Wnt regulator genes were found uniquely upregulated in D538G cells. Interestingly, none of these targets gained ER binding peaks at proximal regulatory regions, suggesting potential epigenetic regulation, which was confirmed in ATAC-seq results. Knockdown of FOXA1, a well-characterized pioneer factor, decreased canonical Wnt activity and abrogated the D538G-unique cell migration in short-term. Conclusion: T47D-D538G cells showed uniquely enhanced cell migration via Wnt hyperactivation, potentially mediated via epigenetic remodeling. These findings suggest the further study of potential combinatorial targeting of Wnt and ER signaling in D538G ESR1 mutant tumors. Citation Format: Zheqi Li, Kevin M. Levine, Spencer Arneson, Kristofer C. Berrett, Nolan M. Priedigkeit, Amir Bahreini, Jian Chen, Li Zhu, Jason S. Carroll, George C. Tseng, Peter C. Lucas, Jennifer M. Atkinson, Jason Gertz, Adrian V. Lee, Steffi Oesterreich. Estrogen receptor D538G mutation promotes cell migration via hyperactivation of Wnt signaling pathway [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 4917.

A Salting-out Liquid-Liquid extraction (SALLE) for the analysis of caprolactam and 2,4-di-tert butyl phenol in water and food simulants. Study of the salinity effect to specific migration from food contact materials

Caprolactam and 2,4-di-tert-butyl phenol (2,4-DTBP) are substances typically found in some food contact materials (FCMs). They are known to often migrate into food, and are difficult to analyse in liquid food simulants using GC. In this work a simple salting-out Liquid-Liquid Extraction (SALLE) for the analysis of both substances in water and the official food simulant A (10 % v/v ethanol, Regulation (EU) No. 10/2011) is presented. The method, which included analytical determination by GC-MS, was optimized and validated to ensure sufficient analytical quality.The method’s LOQs allowed the proper quantification of caprolactam at its EU legislative limit (15 mg kg−1). For 2,4-DTBP the method also revealed good sensitivity, although no official limits have been established yet. Linear regression coefficients (R2) were in all cases higher than 0.999, and recoveries ranged from 87 % and 95% for caprolactam and 2,4-DTBP, respectively. Precision was also acceptable, with the RSDs (%) below 12 %. The method proved to be adequate to be used for routine analysis.The presence of salt during migration of caprolactam and 2,4-DTBP was also investigated in this work. Polyamide/polyethylene FCM multilayer films have been tested with water and simulant A, containing different amounts of NaCl (up to 15 % m/v), and applying different migration conditions (temperature and time). The results indicated that salinity plays an important effect on the migration of caprolactam, with the presence of salt reducing its migration in case of water and increasing it in case of simulant A. These preliminary results seem to indicate that migration testing should consider not only the well-known fatty content of a food, but also its salinity content, as it may end up affecting drastically the migration of polar substances.

The Effects of Severe Plastic Deformation and/or Thermal Treatment on the Mechanical Properties of Biodegradable Mg-Alloys

In this study, five MgZnCa alloys with low alloy content and high biocorrosion resistance were investigated during thermomechanical processing. As documented by microhardness and tensile tests, high pressure torsion (HPT)-processing and subsequent heat treatments led to strength increases of up to 250%; as much as about 1/3 of this increase was due to the heat treatment. Microstructural analyses by electron microscopy revealed a significant density of precipitates, but estimates of the Orowan strength exhibited values much smaller than the strength increases observed. Calculations using Kirchner’s model of vacancy hardening, however, showed that vacancy concentrations of 10−⁵ could have accounted for the extensive hardening observed, at least when they formed vacancy agglomerates with sizes around 50–100 nm. While such an effect has been suggested for a selected Mg-alloy already in a previous paper of the authors, in this study the effect was substantiated by combined quantitative evaluations from differential scanning calorimetry and X-ray line profile analysis. Those exhibited vacancy concentrations of up to about 10−3 with a marked percentage being part of vacancy agglomerates, which has been confirmed by evaluations of defect specific activation migration enthalpies. The variations of Young’s modulus during HPT-processing and during the subsequent thermal treatments were small. Additionally, the corrosion rate did not markedly change compared to that of the homogenized state.

Specific migration of caprolactam and infrared characteristics of a polyamide/polyethylene composite film for food packaging under conditions of long‐term storage before use

Caprolactam is the monomer typically used for the production of polyamide 6 films. Originating from incomplete polymerization reactions, caprolactam can remain part of the final food packaging and has the potential to migrate from the plastic packaging into the packed good. For film and food producers as well as for end users, it is of great interest that the conformity of a film with migration limits is ensured over its lifetime and that environmental conditions do not affect the migration behavior inadequately. In this work, we have investigated the long‐term storage characteristics of a polyamide/polyethylene (PA/PE) film over a storage period of 40.5 months with respect to the specific migration rate of caprolactam. Within statistical indicators, this rate is stable over the complete storage period. Different exposure conditions were applied to the same PA/PE film to accelerate the aging process. Alterations under forced aging conditions (elevated temperature and ultraviolet radiation) were studied by Fourier transform infrared (FTIR) spectroscopy to detect possible chemical changes in the polymer bulk and, by the use of the attenuated total reflection method, on the sample surface. The film was subjected to an extraction study to verify the results obtained using FTIR. Additionally, mechanical tensile tests were carried out. Indication for alterations regarding caprolactam under the different storage conditions were found. The results clearly show that photo‐oxidative conditions led to changes in the chemical and mechanical properties of the film. New chemical species occurred caused by degradation of the polymers and their contribution to chemical migration is discussed.

Ordinary Places of Postmigrant Societies: Dealing with Difference in West and East German Neighbourhoods

The starting point of the contribution is the question of how the dynamics of social encounters in the city are shaped by specific migration histories, local discourses, economies and policies. Against this background, the article analyses the perceptions and localised practices in dealing with social difference and diversity in a comparison of East and West German neighbourhoods. However, this is not about a hierarchizing evaluation, but about understanding urban encounters in the migration society as contestations of social and class recognition, which are played out at different levels and in specific urban places. Based on narrative interviews and field observations, it is shown how urban coexistence is experienced and negotiated in everyday settings between routines and new conflicts. A postmigrant perspective—as a heuristic point of entry—aims to take hegemonic understandings of societal belonging and exclusion under migration-related conditions into question.

Active packaging for table grapes: Evaluation of antimicrobial performances of packaging for shelf life of the grapes under thermal stress

Abstract The paper reports the formulation of an active packaging based on PET coated with a Layered Double Hydroxide (LDH) hosting 2-acetoxybenzoic anion (salicylate) as antimicrobial molecule. The release of the molecule anchored to the LDH, compared to the molecule free dispersed into the coating, appeared much slower. Permeability of carbon dioxide and oxygen through the packaging at different temperatures was evaluated, as well as the capability of the active material to inhibit Pseudomonas, Listeria and Lactobacillus. Table grape was stored in thermal stress conditions (i.e. 10 °C) into the active packaging and the total mesophilic aerobic count and yeasts and moulds population was evaluated up to 14 days of storage. The experimental results were used for a theoretical prediction of shelf life of the packed grapes and compared with the same fruit packed into untreated material. Global and specific migration of salicylic acid from the active packaging demonstrated, in compliance with the migration limits of the EU regulation, the suitability of the considered material for food contact.

Completeness of mortality reports in the vital statistics system in the Colombian Orinoquia, 2017

Estimate the integrity, in terms of completeness, of the mortality records in the vital statistics information system of the Orinoquia and its departments. A descriptive study was carried out, with a secondary source of information, from the records of non-fetal deaths reported in the vital statistics systems of each department, using methods of distribution of the mortality of Brass, Preston-Coale, Hill and Bennet-Hourichi to estimate the integrity of the records. Completeness was found in the mortality records of women and men (≥95%) in Meta, and in the men of Orinoquia and Arauca (≥95%), in contrast to the records of the rest of the territorial units, where under-registration is presented. Both demographic dynamics and migration influenced the results obtained from the integrity of the mortality records, therefore, also in the selection of the most appropriate method to estimate integrity; when the population is neither stable nor closed, methods based on two censuses with specific growth rates and migration adjustment should be applied; if there is no information on migration, the Hill method is recommended.

Políticas de control de la movilidad humana. Las expulsiones colectivas

En este artículo se desarrollará un análisis, delimitado al contexto europeo, sobre cómo las expulsiones colectivas representan una vulneración de derechos humanos, cuál es su impacto y cuál su tratamiento jurídico y jurisprudencial. Para ello se analizarán tres aspectos concretos: en primer lugar, el devenir de la “securitización” de las políticas de control migratorio y su externalización. En segundo lugar, se realizará un estudio de las expulsiones en general, como política de control migratorio, para analizar el caso específico de las expulsiones colectivas. Y, en tercer lugar, se analizará la política migratoria concretada a partir de la firma de convenios de readmisión de la UE o los Estados miembros con países de tránsito y origen, haciendo una breve mención al convenio bilateral firmado por España con Marruecos, con especial atención a la puesta en práctica y la regulación normativa de las devoluciones en caliente.

The migration of bisphenols from beverage cans and reusable sports bottles.

A precise and accurate GC-MS/MS method with ng L-1 LLOQs, acceptable recovery (78-107%) and estimated uncertainty (U > 20%, except at LLOQ) was developed following the Eurachem guidelines. We established the migration and stability of twelve bisphenols in two food simulants (C: 20% ethanol, and B: 3% acetic acid) from beverage cans (n = 16) and reusable metal and plastic sports bottles (n = 51). Bisphenols were stable in dried (eight weeks, -20 °C) and derivatised extracts (seven days, 21 °C). Cans leached BPA (<5865 ng L-1), three BPF isomers (8.2-1286 ng L-1) and BPAP (1.6 ng L-1), while bottles leached BPA (<222 ng L-1) and BPF, BPE, BPB and BPZ (1.1-4.6 ng L-1). Simulant C was more aggressive than simulant B, and concentrations of bisphenols decreased with consecutive exposure to simulants. Levels of BPA migrating from cans did not exceed the specific migration limits.

Fabrication of Stiffness Gradients of GelMA Hydrogels Using a 3D Printed Micromixer.

Many properties in both healthy and pathological tissues are highly influenced by the mechanical properties of the extracellular matrix. Stiffness gradient hydrogels are frequently used for exploring these complex relationships in mechanobiology. In this study, the fabrication of a simple, cost-efficient, and versatile system is reported for creation of stiffness gradients from photoactive hydrogels like gelatin-methacryloyl (GelMA). The setup includes syringe pumps for gradient generation and a 3D printed microfluidic device for homogenous mixing of GelMA precursors with different crosslinker concentration. The stiffness gradient is investigated by using rheology. A co-culture consisting of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and human umbilical cord vein endothelial cells (HUVECs) is encapsulated in the gradient construct. It is possible to locate the stiffness ranges at which the studied cells displayed specific spreading morphology and migration rates. With the help of the described system, variable mechanical gradient constructs can be created and optimal 3D cell culture conditions can be experientially identified.

Regulating and intervening act of Cr chemical speciation effect on the electrokinetic removal in Cr contaminated soil in arid area

Heavy metals chemical speciation significantly effects the electrokinetic removal of heavy metals contaminated soil, but how to regulate and intervene the chemical speciation for improving the removal efficiency in the electrokinetic remediation of the contaminated soil is still an opened problem. In this study, we used increasing citric acid electrolyte concentration and soil pretreatment as regulating measures and the anolyte circulation and reduction wells as intervening act to explore the detailed transformation and migration of Cr speciation over time during electrokinetic remediation, the effect of the transformation and migration of Cr speciation in arid area contaminated soil on the removal efficiency. Based on the results of sequential extraction, the regulating measures can significantly improve the soil pH and the transformation of Cr speciation in arid areas, but the detailed transformation occurs in the early stage of remediation. The material flows showed that the transformation of other speciation to exchangeable (EXC) was higher than its migration to the electrode chamber and the EXC was not completely removed throughout the experiment. By adding HCl to the soil prior to the electrokinetic remediation, more Cr (III) can migrate to the cathode region. Regulating measure for pretreatment of real contaminated soil in arid area based on high concentration of citric acid and intervening act for the anolyte circulation could significantly reduce the bound to Fe/Mn oxides (FM), but the overall removal efficiency of the real contaminated soil (from 44.86% to 44.90%) was not improved, while the removal efficiency of artificially contaminated soil was higher. Furthermore, the multivariate linear regression analysis of the link between the five fractions and removal efficiency of Cr also shows that their correlation was much higher in artificially contaminated soils than in real contaminated soils. The scale effect would affect the transformation of the speciation, and the smaller the scale of the experiment is, the larger the influence on the migration and transformation of Cr speciation will be. The combination of regulating measures and intervening act provide a valuable reference for the specific transformation and migration of heavy metals speciation and the improvement of removal efficiency during EKR of contaminated soil in arid area.

Chemical Migration from Beverage Packaging Materials—A Review

The packaging of a beverage is an essential element for customer convenience and the preservation of beverage quality. On the other hand, chemical compounds present in the packaging materials, either intentionally added or non-intentionally, may be transferred to the food. With a huge variety of materials used in the production, beverage packaging requires safety assessments with respect to the migration of packaging compounds into the filled beverages. The present article deals with potential migrants from different materials for beverage packaging, including PET bottles, glass bottles, metal cans and cardboard multilayers. The list of migrants comprises monomers and additives, oligomers or degradation products. The article presents a review on scientific literature and summarizes European food regulatory requirements. The review shows no evidence of critical substances migrating from packaging into beverages. Testing the migration in real beverages during and at the end of the shelf life shows compliance with the specific migration limits. Accelerated testing using food simulants, however, shows higher migration in some cases, especially at high temperatures in ethanolic simulants. For some migrants, more realistic testing conditions should be applied in order to show compliance with their specific migration limits.